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Guidesheet and Formulas Learly Label All Containers with Content Name Biological Abbreviations GuideSheet and Formulas learly label all containers with content name. Write out the complete chemical and/or biological name or use abbreviations (useful for small containers). If abbreviations are used, display this guide sheet prominently at C several locations in each lab. Extend the list with lab-specific abbreviations, as necessary. Full Name/Formula Abbreviation Full Name/Formula Abbreviation 2-(N-morpholino)ethanesulfonic acid MES Tris/borate/EDTA TBE 3-(N-morpholino)propanesulfonic acid MOPS Tris/EDTA TE 3-[(3-Cholamidopropyl) Tris/EDTA/sodium chloride TEN CHAPS dimethylammonio]-1-propanesulfonate Tris/glycine/SDS TGS 4-(2-hydroxyethyl)-1- HEPES piperazineethanesulfonic acid Tris/phosphate/EDTA TPE Bovine serum albumin BSA Tris/sodium chloride/Tween 20 TNT Dulbecco's Modified Eagle Medium DMEM Tween 20/TBS TTBS Dulbecco's phosphate-buffered saline DPBS Glycerol, Tris-HCl, EDTA, Bromphenol 6 x DNA Loading dye Blue, Xylene Cyanol FF (DNA Agarose gels) (RT) Ethylene glycol-bis(β-aminoethyl EGTA ether)-N,N,N′,N′-tetraacetic acid Protein A/G A/G (4°) Ethylenediaminetetraacetic acid EDTA Alkaline Phosphatase AP (4°) g,4-Dihydroxy-2-(6-hydroxy-1- Fetal bovine serum FBS BFA – Brefeldin A heptenyl)-4 cyclopentanecrotonic acid Solution (4°) Hanks balanced salt solution HBSS l-lactone HEPES-buffered saline solution HeBS 2-Bis(2-hydroxyethyl)amino-2- Bis-Tris (RT) (hydroxymethyl)-1,3-propanediol piperazine-N,N′-bis(2-ethanesulfonic PIPES acid) Caesium chloride (used for plasmid CsCl2 (RT) prep) Phosphate-buffered saline PBS Disodium 3-(4-methoxyspiro Phosphate-buffered saline x Tween 20 PBST {1,2-dioxetane-3,2′-(5′-chloro)tricyclo CSPD (4°) Polyethylene glycol sorbitan [3.3.1.13,7]decan}-4-yl)phenyl TWEEN monolaurate phosphate Denhardt’s solution Sodium chloride/TRIS/EDTA STE Ficoll, PVP, BSA (-20°) Sodium dodecyl sulfate SDS Digoxigenin DIG (4°) Sodium chloride/sodium citrate SSC dNTPs (including dATP, Tris (hydroxymethyl) aminomethane Tris Deoxyribonucleotide triphosphates dTTP, dCTP, dGTP, dUTP) (-20°) Tris (hydroxymethyl) aminomethane Tri-HCl hydrochloride Sodium Deoxycholate solution DOC (4°) Tris buffered saline x polysorbate 20 TBST Ficoll/ Ficoll-Paque (4- Polysucrose 25°) Tris buffered saline TBS Horseradish peroxidase HRP (4°) Tris/acetate/EDTA TAE OFFICE OF ENVIRONMENTAL HEALTH & SAFETY [email protected] | HTTP://EHS.USC.EDU | 323.442.2200 01/2019 Biological Abbreviations and Formulas Guide Sheet Page 2 Full Name/Formula Abbreviation Full Name/Formula Abbreviation Isopropyl thiogalactoside IPTG (4°) MEDIA Lipopolysaccharides (usually E. coli LPS (4°) Brain Heart Infusion (broth or agar) BHI derived) Cell culture media named after Ham’s-F12 Luciferase Luc (-20°) inventor nonyl phenoxypolyethoxylethanol NP40 Luria-Bertani (broth or agar) LB p-nitrophenyl phosphate PNPP Roswell Park Memorial Institute RPMI Medium Polyvinlypyrrolidone PVP Super Optimal Broth SOB Saline, Sodium Phosphate, EDTA SSPE Super Optimal Broth with catabolite 3,3’,5,5’ – tetramethylbenzidine TMB SOC repression N-(2-Hydroxy-1,1-bis(hydroxymethyl) Tricine Trypticase Soy Broth TSB ethyl)glycine Polyethylene glycol p-(1,1,3,3- Triton-X tetramethylbutyl)-phenyl ether Full Name Formula Tetrodotoxin TRX BUFFERS Uracil-DNA N-glycosylase UNG/UDG Na2CO2, NaHCO3, water Coating buffer (for ELISA) 5-bromo-4-chloro-3-indolyl-b-D- X-Gal galactopyranoside PBS, BSA or FBS, EDTA, sometimes with FACS Buffer – (Flow sodium azide, recipes may vary by lab Cytometry buffer) Non-denaturing: Tris HCl, NaCl, NP40, ANTIBIOTICS EDTA. Detergent free: EDTA, Sodium IP Buffer / Co-IP Buffer Ampicillin Amp or Ap azide. Recipes may vary by lab NP40, NaCl, Sodium deoxycholate, SDS, Carbenicillin Carb or Cb RIPA Buffer Tris, water Chloramphenicol Cam or Cm Geneticin G418 WESTERN BLOT BUFFERS Gentamycin Gen or Gm Bromphenol Blue, Kanamycin Kan or Km Laemmli Loading Buffer/Dye Tris-HCl, Glycerol, Neomycin Neo or Nm β-Mercaptoehtanol Penicillin Pen or Pc Running Buffer Tris Base, Glycine SDS Tris Base, Glycine, water, Rifampicin Rif, Ra or Rmp Transfer Buffer Methanol Streptomycin Strep, Str or Sm Tetracycline Tet or Tc OFFICE OF ENVIRONMENTAL HEALTH & SAFETY [email protected] | HTTP://EHS.USC.EDU | 323.442.2200 01/2019 Biological Abbreviations and Formulas Guide Sheet Page 3 Full Name Formula Full Name Formula NORTHERN BLOT BUFFERS With Mercury (Hg) SSC, Formamide, Mercury chloride, Denhardt’s solution, sodium acetate B-5 fixative Hyb and Pre-Hyb buffer SDS, Salmon Sperm DNA anhydrous, (Hyb Buffer also with formaldehyde Dextransulfate) Water, mercury chloride, Formamide, potassium dichromate, Loading/Sample buffer Helly’s fixative Formaldehyde, MOPS sodium sulfate, formaldehyde Tris, EDTA, Na- Stripping buffer pyrophosphate, Water, mercury chloride, Denhardt’s solution Zenker’s fixative potassium dichromate, glacial acetic acid SOUTHERN BLOT BUFFERS EDTA, Phosphoric acid, Lab-Specific Biological Abbreviations and Formulas Church’s buffer sodium phosphate, SDS Denaturation and blotting solution NaOH, NaCl Full Name Formula Hybridization buffer Stringency wash buffers (high, low) SSC, SDS HISTOLOGY STAINING & FIXING SOLUTIONS Without Mercury (Hg) Picric acid, Bouin’s solution formaldehyde, glacial acetic acid Ethanol, chloroform, Carnoy’s solution glacial acetic acid Ethanol, glacial acetic Clarke’s solution acid Ethanol, picric acid, Gendre’s solution formaldehyde, glacial acetic acid H&E stain Hematoxylin & Eosin Y Copper acetate, picric Hollande’s solution acid, formaldehyde, acetic acid Methanol, chloroform, Methacarn glacial acetic acid OFFICE OF ENVIRONMENTAL HEALTH & SAFETY [email protected] | HTTP://EHS.USC.EDU | 323.442.2200 01/2019.
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