Status: Development of Mapping Popula ons
Sco E. Schlarbaum, University of Tennessee and Mark V. Coggeshall, University of Missouri
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 509
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Northern red oak (Q. rubra) mapping parents
SM2 SM1
Comparative Genomics of Hardwood Tree Species 3 http://www.hardwoodgenomics.org Northern red oak mapping popula on
A total of 509 full sibs derived from crossing SM1 and SM2 at Purdue University. Open pollinated seeds collected in 2001, 2003, 2007 and 2010. Full sibs iden fied using paternity exclusion (PE). Full sibs established at Purdue and at the UT Ames Planta on in western TN.
Comparative Genomics of Hardwood Tree Species 4 http://www.hardwoodgenomics.org Northern red oak mapping popula on
In 2013, we started to gra all of the 509 NRO full sibs in Missouri. This clonal popula on will serve as a “back-up” to seedlings popula ons previously established and provide addi onal phenotyping opportuni es for the purpose of developing the first gene c linkage map for the species.
Comparative Genomics of Hardwood Tree Species 5 http://www.hardwoodgenomics.org Oak leaf morphology: QTL mapping in Q. rubra
In 2013, we re-propagated 265 Q. rubra LW full sibs, plus both parents. For each tree, we measured six different leaf NV morphology traits, using two mature, first LL flush leaves from three gra ed ramets. SW WP This dataset will allow us to map QTLs associated with leaf morphology traits in Q. rubra for the first me.
PL
Comparative Genomics of Hardwood Tree Species 6 http://www.hardwoodgenomics.org Oak leaf morphology: QTL mapping in Q. rubra
Comparative Genomics of Hardwood Tree Species 7 http://www.hardwoodgenomics.org Oak leaf morphology: QTL mapping in Quercus robur
Comparative Genomics of Hardwood Tree Species Source: Gailling 2008. QTL analysis of leaf morphological characters in a Quercus robur full-sib family 8 (Q. robur · Q. robur ssp. slavonica). Plant Biol 10:624-634. http://www.hardwoodgenomics.org Oak flowering: QTL mapping in Q. rubra
Comparative Genomics of Hardwood Tree Species 9 http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Fagales: Black walnut Magnoliales (Juglans nigra) Laurales
‘Schessler’ ‘Sparrow’ Monocots Angiosperms Proteales Vitales
Iden fica on of full sibs using paternity Malphigiales exclusion (PE) has been an integral part of Fabales
Eudicots Rosales the MU breeding program since 2009, resul ng in the iden fica on of 650+ full Fagales Myrtales sibs, including 303 ‘Sparrow’ x ‘Schessler’ eudiocts Brassicales Rosids Malvales seedlings (as of summer 2013).
Core Sapindales Cornales
Lamiales Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Fagales: Black Walnut (Juglans nigra) Mapping Popula on
Established in spring 2011, with addi onal seedlings planted in spring 2012.
Total of 303 full sibs successfully established to date. Total of 370 full sibs represented as 2011 DNA samples in ND library.
The size of this mapping popula on will be increased in 2014 with the addi on of ~30 full sibs from the Carlson lab.
Addi onal o.p. seeds were collected in September 2012 for future paternity 2012 exclusion, if needed.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Proteales: Sweetgum Amborellales (Liquidambar styraciflua) Magnoliales Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales Eudicots
Rosales
eudiocts Fagales Myrtales Brassicales Rosids Malvales Core Sapindales Cornales
A new seed-bearing SWG mother tree (MT) was selected in 2011 and seedlings produced Lamiales in 2013. Stress tes ng at PSU and CU will be conducted in 2014. Asterids Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Lamiales: Green ash Magnoliales (Fraxinus pennsylvanica) Laurales
Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales
Fagales Myrtales The development of a GRA mapping eudiocts Brassicales Rosids Malvales popula on was part of the original NSF
Core Sapindales proposal related to the current project. Cornales
However, PE of the 700+ (1+1) seedlings currently available has not been ini ated Lamiales due to funding constraints. Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Lamiales: Green ash (Fraxinus pennsylvanica) In 2012, We completed DNA sampling of the MT to facilitate gene c marker development at ND.
Completed DNA sampling of all puta ve males for future PE (if funded).
Mul ple ssue types were 2013 collected from the MT for mRNA extrac on by PSU. Supplied 100 GRA seedlings to CU for stress tes ng.
In 2013, produced 3,000+ addi onal seedlings for rootstocks
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 30 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Magnoliales: Yellow-poplar (Liriodendron tulipifera)
Open pollinated seedlings were grown from an isolated group of three trees in New Franklin, MO to serve as a back 1-2 seedlings (~300) are available at up to the UT mapping popula on, if MU for plan ng in spring 2014. needed.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ No NA 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Sapindales: Sugar maple Amborellales (Acer saccharum) Magnoliales Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales Fagales eudiocts Myrtales Brassicales Rosids Malvales
Core Sapindales Cornales
We collected seeds in fall 2012 from a campus Lamiales tree at MU, resul ng in 150+ seedlings available for stress tes ng at Penn State, Asterids beginning in spring 2014. Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303
Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No NA 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Cornales: Black gum Magnoliales (Nyssa sylva ca) Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales
Fagales Myrtales Black gum was included in an eudiocts Brassicales earlier version of this project, and Rosids Malvales
Core Sapindales efforts to develop a mapping Cornales popula on began in 2009.
In 2013, we provided ~100 Lamiales seedlings to PSU for stress tes ng. Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org