TAXONOMIC STUDIES of NITIDULIDAE (COLEOPTERA: CUCUJOIDEA) in NORTH AMERICA by COURTNEY LYNN BRISSEY (Under the Direction of Jose
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TAXONOMIC STUDIES OF NITIDULIDAE (COLEOPTERA: CUCUJOIDEA) IN NORTH AMERICA by COURTNEY LYNN BRISSEY (Under the Direction of Joseph V. McHugh) ABSTRACT The sap beetle family Nitidulidae is one of the most diverse families of beetles containing 4,500 species in 350 genera worldwide (Ślipiński, Leschen, and Lawrence, 2011). The subfamilies Carpophilinae and Epureainae contain some of the most economically and ecologically significant species which can be difficult to identify using current literature. I provide an overview of features and technical specifications used to build an interactive web-based key for identification of Carpophilinae (Coleoptera: Nitidulidae) in eastern North America. The terminal taxa list used in the key represents the most current account of carpophiline diversity known to occur in the area. I also provide redescription of the larvae and first description of the pupae of Epuraea ocularis Fairmaire (Nitidulidae), and diagnosis for the adult. Habitus and character images used for the descriptions are provided. New state records for this non-native sap beetle are reported for Georgia and California, illustrating a large range expansion across North America. INDEX WORDS: Morphology, Sap beetle, Characters, Taxonomy, Determinations, Interactive key, Identification key, Multi-entry key, Matrix key TAXONOMIC STUDIES OF NITIDULIDAE (COLEOPTERA: CUCUJOIDEA) IN NORTH AMERICA by COURTNEY LYNN BRISSEY B.S., The University of Georgia, 2012 M.F.R., The University of Georgia, 2015 A Thesis Submitted to the Graduate Faculty of The University of Georgia in Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE ATHENS, GEORGIA 2018 © 2018 Courtney Lynn Brissey All Rights Reserved TAXONOMIC STUDIES OF NITIDULIDAE (COLEOPTERA: CUCUJOIDEA) IN NORTH AMERICA by COURTNEY LYNN BRISSEY Major Professor: Joseph V. McHugh Committee: William G. Hudson Brett R. Blaauw Electronic Version Approved: Suzanne Barbour Dean of the Graduate School The University of Georgia August 2018 DEDICATION To my parents, Johnny and Lisa Brissey, for providing continuous support and always making me feel like I could do anything I set my mind to. And to my boyfriend Aaron Di’Lorenzo, who went above and beyond in facilitating my pursuit of this degree. iv ACKNOWLEDGEMENTS I am forever grateful for all of the wonderful people and organizations who supported my endeavors in completing this degree. First, I cannot thank my M.S. advisor Joseph V. McHugh enough for being a fantastic mentor, teacher, and source of continuous support and guidance. I thank the rest of my M.S. advisory committee, W.G. Hudson and B.R. Blaauw, for providing feedback for this thesis and career advice. I would also like to thank the Department of Entomology and our department head, Dr. Kris Braman, for providing opportunities to learn, through travel and research funding, and for taking a genuine interest in my career aspirations. I am thankful to the members of the McHugh lab (B. Hounkpati, T. McElrath, C. Fair, K. Murray, B. Clark, and T. Sheehan) for their support and feedback. I thank E.R. Hobeke (UGCA) and students from the Department of Entomology (C. Fair, B. Hounkpati, and C. Higashi) for beta testing the Carpophilinae-ID key. I thank my coauthors who helped in the completion of these projects as well. This work was partially supported by grants from the H.H. Ross Fund and from the Georgia Peach Council. We thank J. Grant and B. Gochnour for collecting many of the Georgia samples from outside of Clarke and Oconee County. We also thank F.W. Shockley of the Smithsonian Institution National Museum of Natural History (NMNH), T.C. McElrath of the Illinois Natural History Survey Insect Collection (INHS-INHSIC), and P.E. Skelley and K. Schnepp of the Florida State Collection of Arthropods (FSCA) for loans of authoritatively determined specimens. v TABLE OF CONTENTS Page ACKNOWLEDGEMENTS .................................................................................................v LIST OF FIGURES ......................................................................................................... viii CHAPTER 1 INTRODUCTION AND LITERATURE REVIEW ........................................1 2 CARPOPHILINE-ID: AN INTERACTIVE MATRIX-BASED KEY TO THE CARPOPHILINAE (COLEOPTERA, NITIDULIDAE) OF EASTERN NORTH AMERICA ..........................................................................................4 2.1 Abstract ..............................................................................................5 2.2 Introduction ........................................................................................5 2.3 Project description .............................................................................7 2.4 Conclusions and future work ...........................................................13 2.5 Acknowledgements ..........................................................................13 3 DESCRIPTION OF EPURAEA (HAPTONCUS) OCULARIS FAIRMAIRE (COLEOPTERA: NITIDULIDAE) LARVAE AND PUPAE, WITH A NOTE ON THEIR RANGE EXPANSION IN NORTH AMERICA .........................15 3.1 Abstract ............................................................................................16 3.2 Introduction ......................................................................................16 3.3 Materials and methods .....................................................................17 3.4 Results and discussion .....................................................................19 3.5 Acknowledgements ..........................................................................27 vi 4 CONCLUSION ................................................................................................34 REFERENCES ..................................................................................................................36 vii LIST OF FIGURES Page Figure 1: E. ocularis Mature 3rd Instar Larvae ..................................................................28 Figure 2: E. ocularis Mature 3rd Instar Larvae ..................................................................29 Figure 3: E. ocularis Mature 3rd Instar Larvae ..................................................................30 Figure 4: E. ocularis Pupa .................................................................................................31 Figure 5: E. ocularis Pupa .................................................................................................32 Figure 6: E. ocularis Pupa .................................................................................................33 viii CHAPTER 1 INTRODUCTION AND LITERATURE REVIEW The sap beetle family (Nitidulidae: Coleoptera) is one of the most diverse groups in the superfamily Cucujoidea, comprising 4,500 species in 350 genera worldwide (Slipinski, Leschen, and Lawrence 2011). The North American sap beetle fauna is represented by 165 species in 35 genera (Habeck 2002). While most sap beetles are either saprophagous or mycetophagous, other feeding strategies exhibited include feeding on flowers, pollen, over ripened fruit, fermenting juices, stored products, decaying plant matter, carrion, honey comb, and other beetles (Habeck 2002). Due to their associations with fruits and stored products, many species have been transported globally through trade routes and are now considered cosmopolitan. Two of the most prevalent, widespread, and destructive subfamilies within Nitidulidae are Carpophilinae and Epuraeinae. Many members of these subfamilies can become pests in agricultural settings, food processing facilities, and stored food products. Carpophilinae is represented by 3 genera and 21 species in eastern North America. Some carpophilines, especially those in the genus Nitops, are anthophagous, feeding on decaying flowers and pollen, and therefore have been studied as potential pollinators (Nadel and Pena 1994, Higuchi et al. 2014). Carpophilines also have been associated with the spread of pathogens, such as Ceratocystis fagacearum (Bretz) Hunt which causes oak wilt disease (Cease and Juzwik 2001). Carpophilines are most often associated with over ripened, rotting, or dried fruits and vegetables; however, many 1 species have the ability to damage healthy fruit and transmit bacterial pathogens, making them pests of fruit industries (Leschen and Marris 2005). They are able to transmit some microorganisms that produce mycotoxins (Dowd 1991, Dowd 1995) and also transport Monilinia spp., which results in brown rot of stone fruits (Kable 1969, Williams and Salles 1986). When carpophilines become pests in crops or food storage facilities, one of the most effective management techniques utilizes trap and kill bait stations (Hossain et al. 2006, Bartelt and Hossain 2006). Effective pesticides are available, however, since sap beetles do not usually arrive until crops are ripening, residues from pesticide use can remain on harvested produce. Initially trap and kill bait stations relied solely on fermenting, rotting fruit, but in the last few decades, species-specific pheromones have been incorporated. The use of fermenting fruit volatiles along with species-specific sap beetle pheromones has resulted in trap and kill bait stations becoming as effective as conventional pesticides in controlling carpophiline populations (Bartelt and Hossain 2010). Since trap and kill bait stations rely on lures containing species-specific pheromones, it is important for the user to identify the sap beetle species involved so the correct pheromone can be