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Diallylthiosulfinate (Allicin), a Volatile Antimicrobial from Garlic (Allium
molecules Article Diallylthiosulfinate (Allicin), a Volatile Antimicrobial from Garlic (Allium sativum), Kills Human Lung Pathogenic Bacteria, Including MDR Strains, as a Vapor Jana Reiter 1, Natalja Levina 2, Mark van der Linden 2, Martin Gruhlke 1, Christian Martin 3 and Alan J. Slusarenko 1,* 1 Department of Plant Physiology, RWTH Aachen University, 52056 Aachen, Germany; [email protected] (J.R.); [email protected] (M.G.) 2 German National Reference Centre of Streptococci (GNRCS), University Hospital RWTH Aachen, 52074 Aachen, Germany; [email protected] (N.L.); [email protected] (M.v.d.L.) 3 Institute of Pharmacology and Toxicology, Medical Faculty of RWTH Aachen University, 52074 Aachen, Germany; [email protected] * Correspondence: [email protected]; Tel.: +49-(0)241-802-6650 Received: 13 September 2017; Accepted: 9 October 2017; Published: 12 October 2017 Abstract: Garlic (Allium sativum) has potent antimicrobial activity due to allicin (diallylthiosulfinate) synthesized by enzyme catalysis in damaged garlic tissues. Allicin gives crushed garlic its characteristic odor and its volatility makes it potentially useful for combating lung infections. Allicin was synthesized (>98% pure) by oxidation of diallyl disulfide by H2O2 using formic acid as a catalyst and the growth inhibitory effect of allicin vapor and allicin in solution to clinical isolates of lung pathogenic bacteria from the genera Pseudomonas, Streptococcus, and Staphylococcus, including multi-drug resistant (MDR) strains, was demonstrated. Minimal inhibitory (MIC) and minimal bactericidal concentrations (MBC) were determined and compared to clinical antibiotics using standard European Committee on Antimicrobial Susceptibility Testing (EUCAST) procedures. The cytotoxicity of allicin to human lung and colon epithelial and murine fibroblast cells was tested in vitro and shown to be ameliorated by glutathione (GSH). -
Evaluation of Antibacterial Activity of Spices and Vegetables Against Bacillus Methylotrophicus Strain Kharuss 0103
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 – 6718, ISSN (Print): 2319 – 670X www.ijpsi.org Volume 2 Issue 7‖ July 2013 ‖ PP.37-42 Evaluation of Antibacterial Activity of Spices and Vegetables against Bacillus methylotrophicus strain Kharuss 0103 Khusro A1, Aarti C2, Preetamraj JP1, Kingsley SJ1 1Department of Plant Biology and Biotechnology, Loyola College, Chennai. India 2Department of Biotechnology, M.S.Ramaiah College of arts, science and commerce, Bangaluru. India ABSTRACT: In this investigation the antibacterial activity of aqueous extracts of commonly used spices and vegetables were assayed against Bacillus methylotrophicus strain Kharuss 0103 isolated from poultry farm. Garlic (Allium sativum) extract showed maximum inhibitory effect on Bacillus methylotrophicus strain Kharuss 0103. Aqueous extracts of Zingiber officinale, Allium cepa, Beta vulgaris and Momordica charantia did not inhibit the growth of tested bacteria. Allium sativum were showing zone of inhibition of 30 mm and 24 mm using Agar well diffusion method and Agar disc diffusion method respectively against this strain. These results suggest that Allium sativum is a potential spice for inhibiting the growth of this bacterial strain isolated from poultry farm. KEYWORDS: Antibacterial activity, Agar well diffusion method, Disc diffusion, Poultry farm bacteria, Spices extract, Vegetables extract. I. INTRODUCTION In recent years food safety concerns have been focused on several pathogens. Man has been using natural products of animals, plants and microbial sources for thousands of years either in the pure forms or crude extracts [1]. Vegetables, herbs and spices are an important part of the human diet. They have been used for thousands of years to enhance the flavour, colour and aroma of food. -
(Amaranthaceae) in Italy. V. Atriplex Tornabenei
Phytotaxa 145 (1): 54–60 (2013) ISSN 1179-3155 (print edition) www.mapress.com/phytotaxa/ Article PHYTOTAXA Copyright © 2013 Magnolia Press ISSN 1179-3163 (online edition) http://dx.doi.org/10.11646/phytotaxa.145.1.6 Studies on the genus Atriplex (Amaranthaceae) in Italy. V. Atriplex tornabenei DUILIO IAMONICO1 1 Laboratory of Phytogeography and Applied Geobotany, Department PDTA, Section Environment and Landscape, University of Rome Sapienza, 00196 Roma, Italy. Email: [email protected] Abstract The typification of the name Atriplex tornabenei (a nomen novum pro A. arenaria) is discussed. An illustration by Cupani is designated as the lectotype, while a specimen from FI is designated as the epitype. Chorological and morphological notes in comparison with the related species A. rosea and A. tatarica are also provided. A nomenclatural change (Atriplex tornabenei subsp. pedunculata stat. nov.) is proposed. Key words: Atriplex tornabenei var. pedunculata, epitype, infraspecific variability, lectotype, Mediterranean, nomenclatural change, nomen novum Introduction Atriplex Linnaeus (1753: 1054) is a genus of about 260 species distributed in arid and semiarid regions of Eurasia, America and Australia (Sukhorukov & Danin 2009). Several names (at species, subspecies, variety and form ranks) were described related to the high phenotipic variability of this critical genus (Al-Turki et al. 2000). As conseguence, misapplication of names and nomenclatural disorders exist and need clarification. In this paper, the identity of the A. tornabenei Tineo ex Gussone (1843: 589) is discussed as part of the treatment of the genus Atriplex for the new edition of the Italian Flora (editor, Prof. S. Pignatti) and within the initiative “Italian Loci Classici Census” (Domina et al. -
Understanding the Weedy Chenopodium Complex in the North Central States
UNDERSTANDING THE WEEDY CHENOPODIUM COMPLEX IN THE NORTH CENTRAL STATES BY SUKHVINDER SINGH DISSERTATION Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Crop Sciences in the Graduate College of the University of Illinois at Urbana-Champaign, 2010 Urbana, Illinois Doctoral Committee: Professor Patrick J. Tranel, Chair Associate Professor Aaron G. Hager Associate Professor Geoffrey A. Levin Assistant Professor Matthew E. Hudson ABSTRACT The genus Chenopodium consists of several important weed species, including Chenopodium album, C. berlandieri, C. strictum, and C. ficifolium. All of these species share similar vegetative morphology and high phenotypic plasticity, which makes it difficult to correctly identify these species. All of these weedy Chenopodium species have developed resistance to one or more classes of herbicides. An experiment was conducted to determine if there is variability in response of Chenopodium species present in the North Central states to glyphosate. Our results indicate variable responses within and among the Chenopodium species. Species such as C. berlandieri and C. ficifolium had higher levels of tolerance to glyphosate than did various accessions of C. album. In another experiment, 33 populations of Chenopodium sampled across six North Central states were screened with glyphosate. The results showed variable responses to glyphosate within and among the Chenopodium populations. In general, the Chenopodium populations from Iowa were more tolerant, but some biotypes from North Dakota, Indiana and Kansas also had significantly high tolerance to glyphosate. Given there are species other than C. album that have high tolerance to glyphosate, and there are Chenopodium populations across the North Central states that showed tolerance to glyphosate, one intriguing question was to whether the Chenopodium populations were either biotypes of C. -
Beta Vulgaris As a Natural Nitrate Source for Meat Products: a Review
foods Review Beta vulgaris as a Natural Nitrate Source for Meat Products: A Review Paulo E. S. Munekata 1,*, Mirian Pateiro 1 , Rubén Domínguez 1 , Marise A. R. Pollonio 2,Néstor Sepúlveda 3 , Silvina Cecilia Andres 4, Jorge Reyes 5, Eva María Santos 6 and José M. Lorenzo 1,7 1 Centro Tecnológico de la Carne de Galicia, Rúa Galicia No. 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain; [email protected] (M.P.); [email protected] (R.D.); [email protected] (J.M.L.) 2 Department of Food Technology, School of Food Engineering, State University of Campinas (Unicamp), Campinas 13083-862, SP, Brazil; [email protected] 3 Departamento de Producción Agropecuaria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Campus Integrado Andrés Bello Montevideo s/n, Temuco 4813067, Chile; [email protected] 4 Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CIC-PBA, 47 y 116, La Plata 1900, Argentina; [email protected] 5 Departamento de Ciencias Agropecuarias y Alimentos, Universidad Técnica Particular de Loja, Calle París, San Cayetano Alto, Loja 110107, Ecuador; [email protected] 6 Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma, Hidalgo 42184, Mexico; [email protected] 7 Área de Tecnología de los Alimentos, Facultad de Ciencias de Ourense, Universidad de Vigo, 32004 Ourense, Spain * Correspondence: [email protected] Citation: Munekata, P.E.S.; Pateiro, M.; Domínguez, R.; Pollonio, M.A.R.; Abstract: Curing meat products is an ancient strategy to preserve muscle foods for long periods. -
Evaluation of Two Sugar Beet Cultivars (Beta Vulgaris L.) for Growth and Yield Under Drought and Heat Conditions
Institute of Plant Nutrition Justus Liebig University Giessen Prof. Dr. S. Schubert Evaluation of two sugar beet cultivars (Beta vulgaris L.) for growth and yield under drought and heat conditions A thesis submitted in partial fulfillment of the requirements for the degree of Doctor in Agriculture Submitted by Fathi Mohamed Fathi Abd-El-Motagally Assiut / Egypt 2004 Approved by the examination commission Dean: Professor Dr. Dr. h.c. W. Friedt 1- Advisor: Professor Dr. S. Schubert 2- Advisor: Professor Dr. K-H. Kogel 1- Examiner: Professor Dr. B. Honermeier 2- Examiner: Professor Dr. D. Steffens To my father in spirit whom I always remember and to my mother and dear sisters for their love and to my wife Mervat who helped me to finish this work and last to my daughter Rana that I wish her a good future. 1 Introduction..............................................................................................................................................................1 2 Objectives...................................................................................................................................................................6 3 Material and Methods ......................................................................................................................................7 3.1 Soil experiments.........................................................................................................7 3.1.1 Evaluation of the effects of K+ and Na+ fertilization on growth of two sugar beet cultivars grown under -
Nutritional, Bioactive and Physicochemical Characteristics of Different Beetroot Formulations
Chapter 2 Nutritional, Bioactive and Physicochemical Characteristics of Different Beetroot Formulations Diego dos S. Baião, Davi V.T. da Silva, Eduardo M. Del Aguila and Vânia M. Flosi Paschoalin Additional information is available at the end of the chapter http://dx.doi.org/10.5772/intechopen.69301 Abstract Beetroot possesses high nutritional value and is considered one of the main dietary sources of nitrate. Nitrate has increasingly attracted the interest of the scientific commu- nity regarding new physiological, nutritional and therapeutic approaches with beneficial effects on the cardiovascular system. These effects can be explained by the possible effect of dietary nitrate in stimulating nitric oxide synthesis. Dietary nitrate can be reduced to nitrite in the oral cavity, which is then decomposed to nitric oxide and other bioac- tive nitrogen oxides in the stomach. Beetroot administration can be conducted by several types of formulations, in order to provide a convenient and alternative source of dietary beetroot, such as beetroot juice or beetroot chips and powder. The challenge in providing a product which, in addition to being rich in nitrate, is attractive and easy to administer, while also being microbiologically safe, is increased by the limited scientific information available concerning the nutritional aspects of beetroot formulations. In this chapter, a brief review on the efficiency of different beetroot formulations on health indicators is conducted, emphasizing the effects following the intake of nitrate-enriched beetroot gel. The metabolic and hemodynamic effects of beetroot formulations in healthy and non- healthy volunteers are also discussed. Keywords: beetroot formulations, nitrate, nitric oxide, phenolic compounds 1. Introduction Lifestyle and inadequate eating habits expose humans to a number of risk factors for the devel- opment of chronic non-communicable diseases (CNCDs). -
Amaranthaceae Amaranth Family
Amaranthaceae Amaranth Family Mostly ruderal annuals, there are 900 species in 65 genera. A single genus reaches Nova Scotia. Their flowers are inconspicuous, green and apetalous, subtended by papery bracts. Flowers are unisexual Page | 108 although the plants are monoecious. The terminal inflorescence is brushlike or axillary. A single lens- shaped achene is produced. Some are flowering ornamentals, such as Celosia and Love-lies-bleeding (Amaranthus caudatus) and others are used for grain. Amaranthus L. Three of 50 species have been introduced into Nova Scotia. Key to species A. A. Plants slender, branching diffusely; flowers in small axillary clusters; Amaranthus albus seeds small, <0.8mm wide. aa. Plants robust, erect; flowers in large terminal inflorescences; seeds >1mm B wide. B. Leaves green beneath; sepals pointed. A. hybridus bb. Leaves whitish beneath; sepals truncate. A. retroflexus Amaranthus albus L. Tumbleweed; amarante blanche An erect herb, its stems are freely branching. Leaves are elliptic or oblanceolate, borne on petioles. Flowers are arranged in dense axillary clusters. July to October, on disturbed soils. Uncommon and appearing as a garden weed or about railways. Collected from Truro, Wentworth, Windsor and Kentville. Ranges from western Canada to Mexico. Introduced throughout most of the continent. 3-2 Amaranthaceae Amaranthus hybridus L. Green Amaranth; amarante verte Tall and robust, its stem reaches to 2m in height, often branching freely. Stems are scaly or lightly pubescent Page | 109 especially in the inflorescence. Flowers are reddish, not showy. Leaves are elliptic and petiolate. August to October. A weed of disturbed soils and cultivated fields. It is limited to a few well-established populations: Morristown and other communities in Kings Co. -
Contrasting Nucleolar Activity in Callus of Beet and Garlic As Visualised by a New Silver Staining Technique
_??_1989 by Cytologia, Tokyo Cytologia 54: 553 -558 , 1989 Contrasting Nucleolar Activity in Callus of Beet and Garlic as Visualised by a New Silver Staining Technique S. A. Armstrong1 and B. V. Ford-Lloyd2 1 Regional Cytogenetics Unit, East Birmingham Hospital , Bordesley Green, Birmingham, UK 2 Dept. of Plant Biology, University of Birmingham , PO Box 363, Birmingham BI5 2TT, UK Accepted August 8, 1988 Successful regeneration of whole plants from callus is dependent upon species and often genotype, as well as upon the presence of growth regulators and medium composition. This is exemplified particularly in beet (Beta vulgaris L.) where the tissue or organs from which re sponsive callus is produced, and particularly the genotype governs the success of regeneration (De Greef and Jacobs 1979, Saunders and Daub 1984, Ford-Lloyd and Bhat 1986). The medium composition, particularly with respect to growth regulators has complete influence on callus production and its ultimate organogenic capacity. This situation contrasts with that of garlic (Allium sativum L.) where the potential for plant regeneration from callus is far less dependent on genotype, or the means by which callus is produced, and where callus is capable of switching to a regeneration phase after extended periods of culture confined to callus proliferation (Khadzir 1987). We have assessed the cytological activity of callus from different sources for nucleolar activity with a view to obtaining a reliable marker for regeneration potential of callus. This we have attempted by studying nucleoli in interphase nuclei of callus cells, using a simplified technique of silver staining based upon the colloidal two-stage method of Howell and Black (1980). -
Beta Vulgaris (Common Beet) Class:Magnoliopsida Order
Beta vulgaris (Common Beet) Class:Magnoliopsida Order: Caryophyllales Family: Amaranthaceae Genus: Beta Species: Beta vulgaris Beet seeds Common Varieties: Bull’s Blood, Golden, Chioggia, Detroit Dark Red How to Save Seed Beets are a biennial crop, meaning they require two years to complete their full growing cycle. However, most growers never see this second stage of life because beets are harvested for food during the first year. The second year heralds seed production. To save the seeds from beta vulgaris, the beets themselves must be overwintered. This process, unique to perennial and biennial crops, requires that the taproot of the beta vulgaris (the edible part of the beet) be stored in a protected place during the winter months. A Seed Saving Guide asserts that the optimal temperature range for winter storage is between 35-38F at 90-95% humidity. The roots may be stored in sawdust or wood shavings to minimize rot. This allows the plant to enter a period of dormancy—during this time, the plant’s energy will be diverted to the next year’s seed production. In Spring, plant the overwintered beets outside in a well-watered trench. Because beets are wind-pollinated, they should be planted in a block formation rather than a straight row to ensure proper pollination. The Seed Saver’s Exchange Seed Saving Guide specifies that the isolation distance (the distance between different varieties of beets) must be over 800 feet. Adhering to this distance is critical—without it, there is potential for varieties to cross-pollinate, meaning the genetic integrity of the beet variety will be compromised. -
Table 4: Pollen-Types in Amaranthaceae by Mittre (1963). Amaranthus-Type
Table 4: Pollen-types in Amaranthaceae by Mittre (1963). Amaranthus-type Amaranthus- type sensu stricto Cyathula-type Celosia-type Pollen Faintly to moderately granulate Prominently granulate Baculariate morphological characters: Sexine pattern Total number of 5 2 2 genera studied Genera Amaranthus spinosus L., Cyathula prostrate (L.) Blume, Celosia argentea L., C. cristata L., A. viridis L., A. tricolor L., C. capitata Moq., Allmania albida (Willd.) R.Br. ex Hook.f., A. hybridus L., Deeringia celosioides R.Br. A. nodiflora (L.) R.Br. ex Wight. A. polygamous L., A. tenuifolius Willd., A. spinosus L., Achyranthes aspera L., A. bidentata Blume, Aerva lanata (L.) Juss., A. sanguinolenta (L.) Blume, A. tomentosa Forssk., A. javanica (Burm.f.) Juss. ex Schult., A. monsoniae (L. f.) Mart., Digera arvensis Forssk., Pupalia lappacea (L.) Juss., P. atropurpurea (Lam.) Moq. 22 Table 4: Continued. Gomphrena-type Gomphrena-type sensu stricto Alternanthera-type Not included in any group Pollen reticulate several meshes, without reticulate with few meshes, without reticulate with several meshes and spinulate morphological spinules spinules characters: Sexine pattern Total number of 1 1 1 genera studied Genera Gomphrena globosa L., Alternanthera sessilis (L.) R.Br. ex DC., Psilotrichum ferrugineum (Roxb.) Moq. G. celosioides Mart. A. repens J.F.Gmel. 2 3 Table 5: Pollen-types in Centrospermae by Nowicke (1975). Type I Type II Type III Pollen morphological 3- Colpate Pantoporate Pantocolpate characters: apertures Family studied Aizoaceae, Cactaceae, Caryophyllaceae, Amaranthaceae, Cactaceae, Basellaceae, Cactaceae, Molluginaceae, Nyctaginaceae, Caryophyllaceae, Chenopodiaceae, Molluginaceae, Nyctaginaceae, Phytolaccaceae, Portulacaceae Dysphaniaceae, Nyctaginaceae, Phytolaccaceae, Portulacaceae Phytolaccaceae, Portulacaceae Genera studied in 11 Amaranthaceae Species studied in Amaranthus spinosus L., Amaranthaceae Aerva leucura Moq., Allmania nodiflora (L.) R.Br. -
Beta Vulgaris: a Systematic Review
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by shahrekord university of medical scinces Available online a t www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (19):404-409 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 Chemistry and pharmacological effect of beta vulgaris: A systematic review Sepide Miraj M.D., Gynecologist, Fellowship of Infertility, Assistant Professor, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran _____________________________________________________________________________________________ ABSTRACT Beta vulgaris is a plant native to Mediterranean, the Atlantic coast of Europe, the Near East, and India belong to Amaranthaceae, Genus Beta, and Subfamily Betoideae. The aim of this study is to overview Chemistry and pharmacological effect of beta vulgaris . This review article was carried out by searching studies in PubMed, Medline, Web of Science, and IranMedex databases up to 201 6.Among 89 found articles, 54 articles were included. The search terms were “Beta vulgaris”, “therapeutic”, and “pharmacological”, "Chemistry ". Various studies have shown that Beta vulgaris possess anti-inflammatory effect, antioxidant Properties, anti-stress effect, anti-Anxiety and anti-depressive effect, anti-cancer, antihypertensive effect, hydrophobic properties, anti-sterility effects. The result of this study have found various constituents of Beta vulgaris exhibit a variety of therapeutic