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RESEARCH DAY Book of Abstracts 2020 EPI RESEARCH DAY RESEARCH DAY EMERGING PATHOGENS INSTITUTE RESEARCH DAY The Southern HIV & Alcohol Research Consortium (SHARC), led by Robert L. Cook, MD, MPH, has a mission to reduce HIV transmission and to improve health outcomes among persons affected by alcohol and HIV in Florida. The cover image shows clusters of persons with HIV infection in Florida, who have nearly Book of Abstracts identical viral genetic sequences. The work comes from a collaboration of SHARC with the Data Intelligence Systems Laboratory, led by Mattia Prosperi, MEng, PhD, and the Florida Department of Health.” Book of Abstracts FEBRUARY 2020 2020 EPI RESEARCH DAY 2020 Table of Contents Letter from the Director ..................................................................... 2 Schedule of Events ............................................................................. 3 Keynote Speakers .............................................................................. 4 Enteric and Foodborne Pathogens ............................................... 5-23 Abstracts 1-14 Influenza and Respiratory Viruses ..............................................24-29 Abstracts 15-18 Parasitic and Fungal Diseases .....................................................30-45 Abstracts 19-32 Tuberculosis and Mycobacterial Diseases ..................................46-49 Abstracts 33-34 Vector-Borne Diseases ..............................................................50-100 Abstracts 35-79 Other Bacterial Pathogens ..................................................... 101-131 Abstracts 80-103 Other Viral Pathogens ......................................................... 132-157 Abstracts 104-123 Other Topic Areas .................................................................. 158-181 Abstracts 124-143 Index ...................................................................................... 182-194 1 EPI RESEARCH DAY 2020 Letter from the Director elcome to the thirteenth annual EPI Research Day! As you W look through the abstracts in this book, and view the associated posters, you should get a feel for the wide range of emerging pathogens-related research conducted by EPI members and collaborators at the University of Florida. In keeping with the interdisciplinary nature of EPI, authors come from seven different UF Colleges. We are also pleased to welcome a number of investigators from outside of UF, including authors/collaborators and guests from other Universities in Florida and neighboring states, and local, state, and federal agencies. This year we have the honor of introducing you to two outstanding investigators who will be providing keynote talks during our afternoon session: Dr. Kathryn Hanley is Professor of Biology at New Mexico State University. Her research is in the area of ecologic niche modeling, with a particular focus on arboviruses and their transmission, including sylvatic transmission cycles. Dr. Philippe Sansonetti, from Institute Pasteur, was originally scheduled to be our second speaker. Unfortunately, due to a family emergency, he was not able to join us today. However, I am very pleased to say that Dr. Derek Cummings, from UF’s Department of Biology, was kind enough to step in at the last minute to give the talk. Dr. Cummings is a UF Preeminence Professor and is internationally recognized for his work on transmission of infectious diseases, with a particular focus on dengue and other arboviruses. We are honored to have him and appreciate his willingness to speak on such short notice. Please visit our website, www.epi.ufl.edu, to join our list-serves, and to keep up with our news, events and seminars throughout the year. And thanks for coming! J. Glenn Morris, Jr. M.D., M.P.H. & T.M. EPI Director and Professor of Medicine 2 EPI RESEARCH DAY 2020 Schedule of Events 9:00 AM – 10:00 AM Registration, Breakfast, and Poster Setup Reitz Union – Grand Ballroom 10:00 AM – 1:00 PM Poster Session Presenters, please stand by your posters 12:00 PM – 12:45 PM Lunch Reitz Union – Grand Ballroom 12:45 PM – 1:00 PM Keynote Assembly Reitz Union – Grand Ballroom 1:00 PM – 1:10 PM Welcome Dr. David Nelson, MD Senior Vice President for Health Affairs, UF President, UF Health Introductions Dr. J. Glenn Morris Director, EPI 1:10 PM – 3:15 PM Keynote Speeches 3:15 PM – 4:00 PM Poster Removal 3 EPI RESEARCH DAY 2020 Keynote Speakers 1:10 – 2:10 Kathryn A. Hanley, Ph.D Regents’ Professor Department of Biology New Mexico State University “Waiting in the Wings: Spillover, Spread and Spillback of Mosquito- Borne Viruses” 2:10 – 3:10 Derek A.T. Cummings, Ph.D Professor, Department of Biology, University of Florida Adjunct Professor, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health “The Ecology of Dengue: Changes from Intervention, Climate and Competition” 4 EPI RESEARCH DAY 2020 Enteric and Foodborne Pathogens Abstracts 01. A LIMITED CONSORTIUM OF MOUSE GUT FLORA CONFERS RESISTANCE AGAINST CLOSTRIDIOIDES DIFFICILE INFECTION James Martin - Department of Medicine, College of Medicine, University of Florida; Daniel Marquina - College of Liberal Arts and Sciences, University of Florida; Gurjit Sidhu - Department of Medicine, College of Medicine, University of Florida; Joan Whitlock - Department of Medicine, College of Medicine, University of Florida; Gary Wang - Department of Medicine, College of Medicine, University of Florida Introduction: Clostridioides difficile infection (CDI) is the most common cause of healthcare-associated infections in US hospitals. C. difficile is responsible for nearly half a million cases of diarrhea and colitis per year resulting in 30,000 deaths. While standard antibiotic therapy is effective, 15-30% of patients will develop a subsequent recurrence. Antibiotics and gut dysbiosis render patients susceptible to CDI, but restoring the gut microbiota by fecal microbiota transplant cures CDI. However, the specific gut microbes critical for C. difficile resistance are not known. Methods: “Firmicutes” donor mice (FD) were generated in ex-GF mice whose gut microbiome was restricted to the class Clostridia. Fecal extracts obtained from FD mice were either diluted serially or passaged anaerobically on common microbiological media. Culturing attempts yielded 33 isolates, and 25 unique taxonomic units (OTUs) were confirmed according to 16S rRNA sequencing on the Illumina Mi-Seq platform. The microbiome of GF mice was then reconstituted and orally gavaged in pairs with serially diluted FD fecal in descending scale. For each FD dilution pair, one animal also received a supplement of the 33 isolates. After a period for stable colonization, they were challenged with a virulent strain of C. difficile VPI 10463. Results: Following C. difficile challenge, GF mice engrafted with the FD 1:66 dilution that were also supplemented with the 33 isolates were completely resistant to CDI, negative for both toxin and pathogen colonization. The 1:66 dilution by itself and all decreasing dilutions and 5 their isolate pairs were susceptible to CDI and unable to prevent long- term C. difficile colonization and toxin expression. Conclusion: Both the combined treatments were predominated by members of Ruminococcaceae and Lachnospiraceae, reinforcing our observation that members of these two families alone are competent to reproduce resistance. Where neither the 33 isolates, nor the 1:66 FD dilution by themselves were able to confer resistance against CDI, as a combination they conferred complete protection in a GF mouse. Future culturing efforts concentrated on the 1:66 FD subset could capture missing OTUs, which combined with the 25 OTUs already obtained, encapsulate the whole microbial element responsible for protection against CDI. A minimal consortium of isolates could then be dissected for genetic and metabolomic contributions that underlie microbial resistance to CDI. 02. CAMPYLOBACTER COLONIZATION, ENVIRONMENTAL ENTERIC DYSFUNCTION, STUNTING, AND ASSOCIATED RISK FACTORS AMONG YOUNG CHILDREN IN RURAL ETHIOPIA Dehao Chen - Department of Environmental and Global Health, Emerging Pathogens Institute, College of Public Health and Health Professions, University of Florida; Sarah L. McKune - Department of Environmental and Global Health, Center for African Studies, College of Public Health and Health Professions, University of Florida; Nitya Singh - Department of Animal Sciences, Emerging Pathogens Institute, College of Agricultural and Life Sciences, University of Florida; Arie Havelaar - Department of Animal Sciences, Emerging Pathogens Institute, College of Agricultural and Life Sciences, University of Florida Background: Undernutrition has been identified as an underlying cause in 45% of all under-five mortality. Livestock farming provides a possible mechanism by which smallholder farmers can meet their household need for animal source foods (ASF) which may reduce the risk of stunting. However, direct/indirect contacts with domestic animals may increase colonization by Campylobacter spp., which has been associated with Environmental Enteric Dysfunction (EED) and stunting. Methods: A cross-sectional study involving 102 randomly selected children between 12 and 16 months of age was conducted in rural 6 eastern Ethiopia. Data on anthropometry, EED biomarkers, Campylobacter colonization, socio-demographic variables and hygiene was collected between September and December 2018. Results: The prevalence of EED and stunting was 50% (40% - 60%) and 41% (32% - 51%), respectively. 56% of children had consumed some ASF in the last 24 hours. 47%
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