Occasional Paper 4: Blue-Green Algae
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3916 CRC OCCASIOANL PAPER 4 14/5/04 8:46 AM Page 1 C M Y CM MY CY CMY K The Cooperative Research Centre for Water Quality and Treatment is an unincorporated joint venture between: • ACTEW Corporation • Australian Water Quality Centre • Australian Water Services Pty Ltd Blue-Green Algae • Brisbane City Council CRC for Water Quality and Treatment • CSIRO Molecular Science Private Mail Bag 3 Their significance • Curtin University of Technology Salisbury SOUTH AUSTRALIA 5108 • Department of Human Services Tel: (08) 8259 0211 and management Victoria Fax: (08) 8259 0228 E-mail: [email protected] • Egis Consulting Australia Pty Ltd within water Web: www.waterquality.crc.org.au • Environmental Protection Agency Queensland supplies • Griffith University Algae Blue-Green 4: Occasional Paper • Melbourne Water Corporation • Monash University • Orica Australia Pty Ltd • Power and Water Authority • Queensland Health Pathology & Scientific Services • RMIT University • South Australian Water Corporation • South East Water Ltd • Sydney Catchment Authority • Sydney Water Corporation • The University of Adelaide • The University of New South Wales The Cooperative Research Centre (CRC) for Water Quality and • The University of Queensland Treatment is Australia's national drinking water research centre. An • United Water International Pty Ltd unincorporated joint venture between 28 different organisations from the Australian water industry, major universities, CSIRO, and • University of South Australia local and state governments, the CRC combines expertise in water • Water Corporation quality and public health. • Water Services Association of The CRC for Water Quality and Treatment is established and Australia supported under the Federal Government's Cooperative Research Centres Program. • Yarra Valley Water Ltd Occasional Paper 4 Composite CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 1 Blue-green algae: their significance and management within water supplies Research of the Cooperative Research Centre for Water Quality and Treatment CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 2 In February 2002, the CRC for Water Quality and Treatment hosted a workshop on blue-green algae in drinking water systems. Over 65 members of the Australian water supply community attended this workshop, which featured speakers from across the CRC’s cyanobacterial research program.The articles in this Occasional Paper summarise the research and issues discussed during the workshop. All articles marked * were originally presented at a workshop hosted by the American Water Works Association Research Foundation (AwwaRF) in 2001, and are copyright AwwaRF.The article authored by Ian Falconer was first printed in the journal Phycologia (Vol 40 no 3,pp228-233), and copyright is held by the International Phycological Society. Cooperative Research Centre for Water Quality and Treatment Private Mail Bag 3 Salisbury SA 5108 AUSTRALIA Telephone: +61 8 8259 0211 Fax: +61 8 8259 0228 E-mail: [email protected] Web site: www.waterquality.crc.org.au Blue-green algae: their significance and management within water supplies. CRC for Water Quality and Treatment Occasional Paper 4 ISBN 187661613X Published by the CRC for Water Quality and Treatment 2002 Papers marked * are copyright © 2001,American Water Works Association. Papers marked ** are copyright © 2001, International Phycological Society.All rights reserved. 2 CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 3 BLUE-GREEN ALGAE:THEIR SIGNIFICANCE & MANAGEMENT Table of Contents Blue-Green Algae:Their Significance and Management within Water Supplies . .5 Dennis Steffensen Toxic Cyanobacterial Bloom Problems in Australian Waters: Risks and Impacts on Human Health** . .7 Ian Falconer Cyanobacterial Toxins – The Australian Perspective on Guidelines and Management* . .15 Michael Burch Algicides for Control of Toxic Cyanobacteria* . .23 Michael Burch, Christopher Chow and Peter Hobson Ecology and Management of Cyanobacteria in Rivers and Reservoirs* . .33 Justin Brookes, Peter Baker and Michael Burch The Application of Genetics and Molecular Biology for Detecting Toxic Cyanobacteria and the Basis for their Toxicity . .43 Brett Neilan Identification of Toxic Cyanobacteria Using DNA Based Technology* . .57 Christopher Saint and Kim Fergusson Instrumental Methods for the Determination of Cyanobacterial Toxins* . .61 Brenton Nicholson and Glen Shaw Water Treatment Options for Cyanobacteria and their Toxins* . .75 Mary Drikas, Gayle Newcombe and Brenton Nicholson 3 CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 4 BLUE-GREEN ALGAE:THEIR SIGNIFICANCE & MANAGEMENT 4 CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 5 BLUE-GREEN ALGAE:THEIR SIGNIFICANCE & MANAGEMENT BLUE-GREEN ALGAE:THEIR SIGNIFICANCE AND MANAGEMENT WITHIN WATER SUPPLIES Dennis Steffensen The Australian Water Quality Centre and the CRC for Water Quality and Treatment Private Mail Bag 3, Salisbury, South Australia 5108 E-mail [email protected] Introduction In February 2002, the CRC for Water Quality and Treatment hosted a workshop on blue-green algae in Blue-green algae are a natural and widespread drinking water systems. Over 65 members of the component of most aquatic systems, including streams, Australian water supply community attended this lakes, estuaries and the sea.They are cyanobacteria but as workshop, which featured speakers from across the they have photosynthetic pigments and they look and CRC’s cyanobacterial research program. The articles in behave like algae, they are commonly called blue-green this Occasional Paper summarise the research and issues algae. discussed during the workshop. Cyanobacteria are widely recognised as sources of The research topics and the key participants are taste and odours in water supplies. Many species impart summarised in Table 1 (below).This represents the most disagreeable earthy or musty taste and odours that comprehensive research program targeted at the needs render the water unpalatable.With the identification of a of the water industry anywhere in the world. As a number of toxic cyanobacterial metabolites there is reflection of that international recognition the CRC has increasing concern for health effects to consumers. It is recently entered into joint funding agreements with the the health risks that are the focus of this workshop. American Water Works Association Research Foundation (AwwaRF) for research into analytical methods for The forms of cyanobacteria that are of most concern cyanotoxins and for the development of genetic methods are those that form dense blooms.These are favoured by for the rapid detection of toxic species. hot calm conditions and high nutrient loads. The combination of climate, the construction of large dams and the regulation of many of the rivers have resulted in Australia experiencing more difficulties with cyanobacteria than most other regions in the world. The importance of cyanobacteria to the Australian water industry is reflected in the prominence given to this issue within the research programs of the CRC for Water Quality & Treatment.The CRC began in 1995 and a bid for a second term was successful in 2001. The research priorities are based on the major concerns of the water industry namely: • Determination of the health risks. • Development of monitoring methods for both the organisms and their toxins, • Understanding of the factors which promote cyanobacterial growth leading to more effective control in source waters, • Development of effective water treatment processes to destroy or remove toxins. 5 CRC - blue greeen algae (21) 14/5/04 8:36 AM Page 6 BLUE-GREEN ALGAE:THEIR SIGNIFICANCE & MANAGEMENT Table 1. Summary of research by the CRC for Water Quality & Treatment Topic Key Researchers Students Health risks • Toxicology • Ian Falconer, • Suzanne Froscio,Adelaide Animals subjected to microcystin Adelaide University University and cylindrospermopsin • Andrew Humpage,Australian Water Quality Centre (AWQC) • Glen Shaw, National Research Centre for Environmental • Epidemiology Toxicology (NRCET) Effects from exposure by drinking • Louis Pilotto, National Centre for water & recreation Environmental and Public Health • Guidelines (NCEPH) Formulation of safe levels for • Ian Falconer, drinking water and recreation. Adelaide University • Mike Burch,AWQC Monitoring • Conventional taxonomy • Peter Baker,AWQC • Genetic/molecular techniques • Chris Saint,AWQC • Kim Fergusson, University of for identifying toxic species. • Brett Neilan, South Australia (UniSA) University of NSW (UNSW) • Melanie Kaebernick, UNSW • Carolina Beltran, UNSW • Daniel Tillett, UNSW • Michell Moffitt, UNSW Toxin Analysis • Analytical methods developed for • Brenton Nicholson,AWQC all the common toxins.Techniques • John Papageoriou,AWQC include: HPLC, Mass • Carolyn Haskard,AWQC Spectrometry, Capillary • Peter Hawkins, Sydney Water Electrophoreois, ELISA, and • Nimal Chandrasena, enzyme inhibition. Sydney Water • Jing Cao, Sydney Water Ecology/Source Water Management • Flow manipulation in rivers • Mike Burch,AWQC • Karen Westwood,Adelaide • Mixing/destratification of • Justin Brookes,AWQC • University reservoirs • Peter Hobson,AWQC • Rudi Regel, • Algicides • Peter Hawkins, Sydney Water • Adelaide University • Dennis Steffensen,AWQC • David Lewis, • Adelaide University • Hugh Wilson, • Adelaide University • David Moore, • University of Queensland Water Treatment • Sam Brooke, UniSA • Removal of cells by convention • Mary Drikas,AWQC • David Cook, UniSA treatment & membranes. • Gayle Newcombe,AWQC