The Performance of Gold-Mouth Turban Turbo Chrysostomus Larvae in Different Temperature and Salinity Media
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Jurnal Akuakultur Indonesia 20 (1), 14–23 (2021) Original article DOI: 10.19027/jai.20.1.14-23 The performance of gold-mouth turban Turbo chrysostomus larvae in different temperature and salinity media Kinerja larva siput mata bulan Turbo chrysostomus pada suhu dan salinitas media yang berbeda Aris Sando Hamzah1*, Kukuh Nirmala1, Eddy Supriyono1, Irzal Effendi1, Sigit A.P. Dwiono2 1Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia 2Central Marine Bio Industry, Indonesian Institute of Sciences (LIPI), Mataram, West Nusa Tenggara, Indonesia *Corresponding author: [email protected] (Received September 22, 2020; Accepted October 16, 2020) ABSTRACT This study aimed to evaluate the effect of temperature and salinity on the development, growth, and survival rate of gold-mouth turban T. chrysostomus larvae. The temperature was adjusted by heater and salinity was by the freshwater dilution. The temperature treatments were A1 (27 ± 0.5°C) and A2 (30 ± 0.5°C), while the salinity treatments were B1 (29 ± 0.5 g/L), B2 (32 ± 0.5 g/L), and B3 (35 ± 0.5 g/L). These treatments were repeated three times. The results showed that the pre-torsion veliger stage was formed at about 11 hours 36 minutes after fertilization or at about 3 hours after the trocophor stage. The A1B3 treatment obtained the fastest early post-torsion veliger and late post-torsion veliger stage period after fertilization. Temperature had no significant effect, while salinity had a significant effect in the specific growth rate of gold-mouth turban larvae. The highest specific growth rate at 27 ± 0.5°C (A1) was obtained in the B3 treatment and insignificantly different from the B2 treatment. Temperature and salinity had a significant effect, but the interaction between these factors had no significant effect in the survival rate of gold-mouth turban larvae. The A1B3 treatment obtained the highest percentage of survival rate and was significantly different from the A1B2 treatment. The optimum temperature and salinity ranges for maintaining the gold-mouth turban larvae are 26.5–27.5°C and 31.5–35.5 g/L, respectively. The water quality parameters obtained could still support the larvae development until the juvenile stage. Keywords: temperature, salinity, larvae, gold-mouth turban ABSTRAK Tujuan penelitian ini yaitu mengevaluasi pengaruh suhu dan salinitas terhadap perkembangan, pertumbuhan, dan kelangsungan hidup larva siput mata bulan T. chrysostomus. Suhu diatur menggunakan heater dan salinitas dengan melakukan pengenceran air tawar. Perlakuan suhu yaitu A1 (27 ± 0.5°C) dan A2 (30 ± 0.5°C), sementara salinitas yaitu B1 (29 ± 0.5 g/L), B2 (32 ± 0.5 g/L) dan B3 (35 ± 0.5 g/L) diulang sebanyak 3 kali. Hasil pengamatan menunjukan bahwa stadia veliger pra-torsi dicapai sekitar 11 jam 36 menit setelah fertilisasi atau sekitar 3 jam setelah trocophor. Perlakuan A1B3 memberikan waktu pencapaian stadia veliger pasca-torsi awal dan veliger pasca-torsi akhir tercepat setelah pembuahan. Suhu tidak berpengaruh nyata sedangkan salinitas berpengaruh nyata terhadap laju pertumbuhan harian larva siput mata bulan. Laju pertumbuhan harian tertinggi pada suhu perlakuan A1 didapatkan pada perlakuan B3 dan menunjukan nilai tidak berbeda nyata dengan perlakuan B2. Suhu dan salinitas memberikan pengaruh signifikan, tetapi interaksi keduanya tidak menunjukan pengaruh yang signifikan terhadap tingkat kelangsungan hidup larva siput mata bulan. Perlakuan A1B3 memberikan persentase tingkat kelangsungan hidup tertinggi dan tidak menunjukan nilai yang berbeda nyata dengan perlakuan A1B2. Kisaran suhu dan salinitas optimum bagi pemeliharaan larva siput mata bulan yaitu suhu 26.5–27.5°C dan salinitas 31.5–35.5 g/L. Parameter kualitas air yang diperoleh masih mendukung perkembangan larva siput mata bulan hingga mencapai stadia juvenil. Kata kunci: suhu, salinitas, larva, siput mata bulan Aris Sando Hamzah et al. / Jurnal Akuakultur Indonesia 20 (1), 14–23 (2021) 15 INTRODUCTION total energy used to balnce the osmotic pressure, the less reserved energy utilized for growth, The gold-mouth turban Turbo chrysostomus is movement, and other biological requirements a quite potential invertebral spesies due to easier (Amin et al., 2016). The giant abalone Haliotis maintenance and having a good survival ability gigantean can adapt to low salinity in a long against the environmental changes (Setyono et period and produce higher survival rate than al., 2013). Gold-mouth turban can be found in the the disc abalone (H. discus discus) (Manuel et Indo-Pacific, South-East Asia, Fiji Islands coastal al., 2019). Morash and Alter (2015) stated that regions. This snail species can also be found in larvae are more easily exposed to diseases due the Ryukyu Islands, Japan coastal region and to temperature and salinity fluctuations. Both Northern Melanesia to Southern New Caledonia parameters can influence the body immune water areas (Soekendarsi, 2018). Gold-mouth system. The optimum temperature and sainity turban often becomes the local community target, interactions in the pearl oyster larvae obtained the due to the savory taste and high nutrient contents. highest survival and growth rates by improving The gold-mouth turban also becomes a popular the protease enzyme activity and a higher CaCO3 aquarium organism. Based on this condition, content than the other treatments (Hamzah et al., gold-mouth turban (T. chrysostomus) has been 2016b). Lah et al. (2016) stated that the biotic rarely found in the Indonesian water areas factors and environmental conditions, namely, (Ubaidillah et al., 2013). Ruf (2007) also added temperature and salinity can also influence the that the fairly high price of the carved shells marine organism fatty acid composition. around IDR 258,000 (US $ 18) per kg is thought The gold-mouth turban breeding often fails to likely occur due to the drastic decline of these due to the water quality change. Temperature and organisms in Sabah water area. Moreover, the salinity can cause egg and larvae abnormalities, coral reef damage and environmental change are inhibited larvae development, and mortality. also thought to be the main factors of the declined High mortality occurs whenever during the population in nature (Seinor et al., 2020). metamorphosis stage; therefore, the fluctuative Culture can become a solution to overcome temperature and salinity in the maintenance these problems. However, the current gold-mouth media extremely influence the gold-mouth turban (T. chrysostomus) culture in Indonesia has turban production (Kimani, 1996; Setyono et al., not been commercially performed due to less 2013). This study aimed to evaluate the effect information about the culture process, specifically of temperature and salinity on the development, in the breeding stage. The larval phase often occurs growth, and survival rate of gold-mouth turban T. a high mortality, either in the early development chrysostomus larvae. (veliger) to the larval final development. The larvae phase is a transitional period, as mishandling and MATERIALS AND METHOD water condition changes can lead to a mortality that directly impacts the seed quantity and quality Experimental design produced. Temperature and salinity are the water This study contained two temperature levels quality parameters that play the important roles and three salinity levels. The temperature in supporting the gold-mouth turban breeding treatments were A1 (27 ± 0.5°C) and A2 (30 ± activity. The optimum temperature can induce 0.5°C), while the salinity treatments were B1 the larval appetite, which implicates in the (29 ± 0.5 g/L), B2 (32 ± 0.5 g/L), and B3 (35 ± larval performance. Meanwhile, the extreme 0.5 g/L), which were repeated three times. The temperature can interfere with the physiological maintenance tank contained 18 units of containers response of larvae and cause a high mortality which were randomly distributed. The container (Huo et al., 2017). In the juvenile phase, gold- tanks used were made of white colored plastic at mouth turban is also influenced by temperature, 73×52×42 cm (P × L× T) volume. which tended to grow faster at 26 ± 0.5°C and were thought to be associated with the high daily Procedures feed intake (Hamzah, 2015). Tank preparation The salinity change can cause the increased The preparatory step began by sanding energy demand, especially in balancing the the maintenance container and plate (larval osmotic pressure between body and environment, attachment medium) until quitely coarse. This which impacts on the energy sharing. The higher aimed to facilitate the Navicula sp. attachment as 16 Aris Sando Hamzah et al. / Jurnal Akuakultur Indonesia 20 (1), 14–23 (2021) a live feed. The plate used was white colored fiber salinity; therefore, to maintain the salinity at 32 ± media at 25×20 cm (P×L) size. After sanding, the 0.5 g/L and 29 ± 0.5 g/L treatments, dilution was maintenance container and plate were cleaned until performed by adding the freshwater. The dilution sterilized and dried. Three plate units were hung procedure was referred to Winanto et al. (2009): in each maintenance container, and the container was filled with the sterilized marinewater. The live feed was proliferated at about 2 weeks before larval stocking by growing Navicula sp. Note: in the larval attachment media. The live feed S = maintained salinity (g/L) grown in the attachment media were cultured by S1 = marinewater salinity (g/L) administering 1 mL/L of KW21 liquid fertilizer S2 = freshwater salinity (g/L) type equipped with a 15 W lamp to stimulate these V1 = marinewater volume (L) diatom growth. KW21 fertilizer is a commercial V2 = freshwater volume (L) fertilizer containing 49 g/L nitrogen (N), 4 g/L phosphoric acid (P), boron, manganese, iron, zinc, The water quality parameters in the cobalt, EDTA, amino acid complex, and vitamins experimental media, mainly temperature and (Mukhlis et al., 2017). For maintaining the live salinity, were monitored gradually twice a day. feed availability in the experimental media, the Moreover, to maintain the experimental water live feed was provided at 20 000 cells after water quality media, water exchange was totally exchange.