HPLC BONDED PHASES | Appendices 379 2 2 F
3 F CH ) 2 F 2 3 ) OHOH CHCH 3 2 OH 2 CH 2 (CH 2 CH + 2 F NH F OCH O N SO 2 CH 2 2 2 2 2 2 2 CH CH CH CH N(CH CH CH CH 2 CH 2 2 2 2 2 2 2 2 CH Structure CH CH CH CH COOH CH CH CH CH=NH 2 CH 2 2 2 2 2 2 2 2 2 2 2 CH CH CH CH CH CH NO CH CH CH CH CH Si Si Si Si Si Si Si Si Si Si Si Si - Linker) O Linker) 12 6 H H 6 3 (C (C 5 5 5 5 H F H H 6 6 6 6 Reversed phase material. Uses a patented Reversed phase material. hexyl linker as opposed to traditional propyl. Offers selectivity of a phenyl phase with greatly enhanced stability. C Reversed phase material. Used to separate Reversed phase material. Alter extremely polar aromatic compounds. Useful for Reversed phase material. analyzing substituted aromatic compounds. classic Alternative selectivity to phenyl-hexyl, and alkyl phases. phenyl phases, Strong Base Quaternary amine, Q, Strong anion- Ion-exchange material. exchangers (basic) are useful for separating acids. and organic nucleosides, nucleotides, Acid Strong Acid, Sulfonic S, Strong cation- Ion-exchange material. exchangers (acidic) are useful for separating organic bases. Base, Weak Diethylaminoethyl, DEAE, Polyethyleneimine PEI, anion- Weak Ion-exchange material. exchangers (acidic) are useful for analyzing and purifying acidic proteins and peptides. Acid Weak Carboxymethyl, CM, cation- Weak Ion-exchange material. exchangers (basic) are useful for analyzing and purifying basic proteins and peptides. APS, Amino, Amino Propyl Silyl Amino Propyl Amino, APS, or weak normal phase, Reversed phase, anion exchange material. Reversed phase: useful for separating carbohydrates. Normal phase: alternative selectivity to not deactivatedamounts by small silica, of water. weak anion-exchanger when Ion exchange: used with buffers; separates anions and organic acids. Nitro Separates aromatic Normal phase material. double compounds and compounds with bonds. Glycerol Diol, material. Reversed phase or normal phase ChromatographyUsed for Gel Filtration (GFC) Normal phase: of proteins and peptides. not deactivated by similar selectivity to silica, small amounts of water. C Useful for analyz- Reversed phase material. When bonded to ing aromatic compounds. this phase is useful for HIC. Å silica, 300 C native selectivity to Phenyl-hexyl and classic phenyl phases. C Description 2 2 PFP SAX SCX WAX WCX NO OH Phenyl Phenyl-Ether Phenyl-Hexyl Phase NH CN 2 CH 2 25 37 11 13 17 9 5 7 CH H H 3 2 H H H H H H 12 18 5 6 8 4 2 3 C C C C C C OH CH C C CH Structure Si Si Si Si Si Si Si Si Si Si Si - - Classic reversed phase material. Most Classic reversed phase material. Excellent for retentive for non-polar solutes. Wide applicabil- ion-pairing chromatography. steroids, nucleotides, nucleosides, ity (e.g., envi- fatty acids, vitamins, pharmaceuticals, When bonded to 300 ronmental compounds). this phase is perfect for separating Å silica, small proteins and macromolecules. Nitrile Cyanopropyl, Cyano, PCN, CPS, Reversed phase or normal phase material. unique selectivity for polar Slightly polar, compounds in both reversed phase and nor Equilibrates very rapidly, mal phase modes. Useful for suitable for gradient separations. many pharmaceutical applications (e.g., antidepressants). tricyclic Reversed phase material. Similar selectivity Reversed phase material. Wide applicability to C18 but less retentive. steroids). nucleosides, pharmaceuticals, (e.g., Dodecyl Shorter chain Reversed phase material. length provides higher bonded phase cover Reversed phase material. Useful for reversed Reversed phase material. phase separation of hydrophobic proteins More hydrolyti- Å silica. when bonded to 300 cally stable than C4. Hexyl Useful for ion- Reversed phase material. pairing chromatography. Octyl LC8, RP-8, MOS, age than most C18 columns for sharp peak shapes of non-polar and moderately polar compounds. Octadecyl LC18, RP-18, ODS, Reversed phase material. Used in Hydro- Used Reversed phase material. phobic Interaction Chromatography (HIC) of proteins and peptides. Butyl for HIC Useful Reversed phase material. Offers less and ion-pairing chromatography. retention than C8 and C18 phases for non- it Å silica, When bonded to 300 polar solutes. is an ideal phase for analyzing and purifying large proteins and macromolecules. Pentyl Classic normal phase material. Suitable Classic normal phase material. for separating polar non-ionic organic compounds. Trimethyl SAS, TMS, selectivity Unique Reversed phase material. for polar and multifunctional compounds. Dimethyl RP-2, Reversed phase material. Propyl Silica Description
CN C12 C6 C8 C18 C4 C5 C1 C2 C3
Phase Si HPLC Bonded Phases Bonded HPLC Phenomenex HPLC Material Sorbent Characteristics | Appendices 385 L1 L1 L7 L3 L1 L7 L1 L1 L1 L1 L7 L3 L8 L1 L1 L1 L1 L1 L1 L7 — — — — L15 L11 L26 L21 L11 L11 USP acking No No No No No No No No No No No No No n/a Yes Yes Yes Yes P Yes, triple Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, Yes, double Yes, End Capping ) 2 pH — — 0-14 Range 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 2.0-9.0 2.0-9.0 2.0-9.0 2.0-9.0 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 2.5-7.5 1.5-7.5 1.5-7.0 1.5-7.5 2.5-9.0 2.5-7.5 2.5-7.5 1.5-7.0 1.5-10.0 1.5-10.0 1.5-10.0 1.5-10.0 — — — — — — — — — — — — — — — — — — — — — — — — — — — No No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes End Coverage (μmole/m Partial Partial Capping Calculated* Bonded Phase Proprietary Proprietary Proprietary Proprietary ) 2 0 — — — — — — — — — — — — — — — — — — N/A 5.00 3.50 2.61 2.93 3.21 2.45 3.15 4.06 3.53 2.71 4 8 8 7 4 4 4 4 % 20 12 10 Coverage 12 10 12 7.6 7.2 5.5 5.5 2.8 1.5 1.1 1.2 9.5 5.5 4.3 (μmole/m 12.5 Calculated* Bonded Phase proprietary Carbon Load % Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric /g) 2 - 7 6 6 0 0 3 2 2, 7, 45 45 45 45 300 300 Carbon Load 160 300 160 300 180 180 300 180 180 300 180 180 180 180 180 180 180 180 180 180 180 12 12 17 19 11 12 17 19 11 — 20, 13, 10, 31, 22, 14, (m 10.0, 15.5, 12.6, 18.5, urface Area Surface /g) 2 — — — — 200 200 200 200 200 450 450 310 310 110 110 410 200 400 400 200 400 200 400 475 475 475 475 550 370 550 Area (m Surface — — — — — — — — — — — — — — — — — — — — — — — — — — — (mL/g) ore Volume P ore — — — — — — — — — — — — — — — — — — — — 1.0 1.1 1.1 P 1.05 1.05 1.05 1.05 0.80 0.75 0.80 (mL/g) Volume 70 70 95 70 95 70 80 80 70 80 80 70 80 80 80 80 80 80 80 80 80 80 80 (Å) 300 300 300 300 ore Size P ore 80 80 80 80 80 80 80 80 80 80 80 80 60 90 60 (Å) 100 100 150 300 150 300 110 110 110 100 110 100 100 100 100 Size P (μm) Spher. 5 Spher. Spher. 5,7 Spher. Spher. 3,5,7 Spher. Spher. 3,5,7 Spher. Spher. 3,5,7 Spher. Spher. 3,5,7 Spher. Spher. 3.5, 5 3.5, Spher. (μm) article Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. Spher. 3.5, 5, 7 5, 3.5, Spher. article Shape/Size article Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5 3.5, 1.8, Spher. P Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. P Spher. 1.8, 3.5, 5, 7 5, 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5, 7 5, 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5, 7 5, 3.5, 1.8, Spher. Spher. 1.8, 3.5, 5, 7 5, 3.5, 1.8, Spher. Spher. 2.5 Spher. 2.5 Spher. Spher. 2.5 Spher. Spher. 2.5 Spher. Shape/Size Spher. 5, 7 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 4, 10 4, Spher. 10 4, Spher. Spher. 4, 10 4, Spher. Spher. 4, 10 4, Spher. Spher. 3, 5, 7 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 5, 10, 15 10, 5, Spher. Spher. 5, 10, 15 10, 5, Spher. Spher. 5, 10, 15 10, 5, Spher. Spher. 3, 5, 7, 10, 15 10, 7, 5, 3, Spher.
2
2 acking Material acking Material SphereClone NH SphereClone ODS (2) SphereClone ODS (1) SphereClone C8 SphereClone C6 SphereClone Silica Prodigy Phenyl (PH-3) Prodigy ODS (3) Prodigy C8 PrimeSphere C4 Prodigy ODS(2) PrimeSphere C18-MC PrimeSphere C18-HC PrimeSphere C18-MC P Zorbax ODS PrimeSphere C8 P RP-1 PolymerX PrimeSphere Si SphereClone SAX Synergi Fusion-RP Synergi Hydro-RP SphereClone Phenyl Synergi Max-RP Synergi Polar-RP Zorbax Eclipse Plus C18 Zorbax Eclipse Zorbax C8 Synergi Fusion-RP Synergi Hydro-RP Synergi Max-RP Synergi Polar-RP Zorbax Eclipse Plus C8 Zorbax Eclipse Zorbax CN Ultracarb ODS (30) Ultracarb ODS (20) Ultracarb C8 Zorbax Eclipse XDB-C18 Zorbax Eclipse Zorbax Phenyl Zorbax Eclipse XDB-C8 Zorbax Eclipse Zorbax Eclipse XDB-Phenyl Zorbax Eclipse Zorbax TMS Zorbax Zorbax Eclipse XDB-CN Zorbax Eclipse Zorbax SB-C18 Zorbax NH Zorbax SB-C8 Zorbax SB-C3 Zorbax SB-Phenyl Zorbax SB-CN Zorbax SB-Aq Zorbax 300SB-C18 Zorbax 300SB-C8 Zorbax 300SB-C3 Zorbax 300SB-CN Zorbax Bonus-RP Zorbax Extend-C18 Zorbax 300 Extend-C18 Zorbax Rx-C18 Zorbax Rx-C8 This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed. nor in terms of their products, This selection is, neither in terms of manufacturers Other Sorbents (HP) Agilent Technologies Phenomenex Sorbents (cont'd) Phenomenex Sorbents This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed. accuracy of data is not a complete list and the products, nor in terms of their is, neither in terms of manufacturers This selection HPLC Material Sorbent Characteristics Sorbent Material HPLC a selection of a selection Phenomenex 386
Appendices | HPLC Material Sorbent Characteristics Hplc MaterialSorbentCharacteristics a selectionof Alltech Associates, Inc. Sciences) /Vydac(GraceDavisoniscovery Other Sorbents(cont'd) This selectionis,neitherintermsofmanufacturers norintermsoftheirproducts, acompletelistandtheaccuracyofdataisnotguaranteed. G.L. Science Eka Chemicals Beckman Coulter Vydac 238 TP (C18) Vydac 219 TP (Phenyl) Vydac 218TP(C18) Vydac 214TP(C4) Vydac 208TP(C8) Vydac 201TP(C18) Vydac 201SP(C18) Prevail Phenyl Prevail C8 Prevail C18 Platinum/Platinum EPS Platinum/Platinum Platinum C8-300 Platinum C8EPS Platinum C8 Platinum Platinum C4-300 Platinum Platinum C18-300 Platinum Platinum C18EPS Platinum Platinum C18 Platinum Everest Denali Apollo Phenyl Apollo Apollo C18 Apollo Apex Phenyl Apex Apex ODS-2 Apex Apex ODS-1 Apex Apex CN Apex Apex C8e Apex Apex C8 Apex Apex C2 Apex Alltima HPHILIC Alltima HPSilica Alltime HPCyano Alltima HPC8 Alltima HPC18 Amide Inertsil ODS(3) Alltima HPC18HiLoad Inertsil ODS(2) Alltima HPEPSC18 Inertsil ODSPrep Alltima HPC18 AQ Inertsil ODS Alltima HPC18 Alltima C8 Inertsil Si Kromasil NH2 Alltima C18 Kromasil C18 Alltima AQ Inertsil C4 Kromasil C8 Kromasil C4 Adsorbosphere HS Inertsil Phenyl Kromasil C1 Adsorbosphere C18 P Inertsil C8 Kromasil Si Ultrasphere acking Material Spher. 3.5, 5, 7, 10, 13, 16 Spher. 3.5, 5, 7, 10, 13, 16 Spher. 3.5, 5, 7, 10, 13, 16 Spher. 3.5, 5, 7, 10, 13, 16 Spher. 3.5, 5, 7, 10, 13, 16 Spher. 5, 7, 10, 13, 16 P Spher. 1.5, 3, 5,10 Spher. 1.5, 3, 5,10 article Shape/ize Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5,10 Spher. 3, 5,10 Spher. 3,5,10 Spher. 3, 5, 7 Spher. 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 5,10 Spher. 5,10 Spher. 5,10 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 10 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 (μm) — — P ore Size 300 300 300 300 300 300 110 110 110 300 300 300 100 100 300 100 100 300 120 100 100 100 100 100 100 100 100 100 120 100 190 190 190 100 100 150 190 100 100 190 150 100 100 100 150 100 100 100 150 100 100 150 100 (Å) 90 80 60 80 80 P ore Volume (mL/g) 0.78 0.78 0.51 0.51 0.78 0.78 0.78 0.51 0.77 0.77 0.77 0.77 0.77 0.77 0.77 0.6 0.6 0.6 0.6 0.6 0.6 0.8 0.9 0.9 0.9 0.9 0.9 0.9 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — Surface Area (m 350 450 350 350 100 100 200 200 100 100 200 200 340 340 170 170 170 170 170 170 170 230 450 200 200 200 450 450 320 200 350 450 450 200 320 340 320 340 340 350 320 340 340 200 320 340 — 90 — 90 90 90 90 90 — — — — 2 /g) 20.0, 12.0, 13.5 10.5 18.5 17.5 10.0 10.5 2.5, 4.7, 15, 7.5 16, 19, 15, 12, — — — — — — — — — — — — 15 10 12 24 15 14 20 12 Carbon Load 8, 3, 8, 7, 8, 4, 5, 6, 9, 8, 5 8 9 4 8 7 2 4 8 4 0 0 % Monomeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Calculated* BondedPhase overage (μmole/m Coverage 4.53 4.16 4.89 4.16 1.53 4.61 2.83 3.57 4.47 3.84 3.43 3.22 3.76 3.27 2.77 2.99 3.26 3.1 3.6 3.8 4.3 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — 3 0 0 2 ) Phenomenex End Capping Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes n/a n/a — — — — No No — — — — — No No HPLC Material Sorbent Characteristics | Appendices 387 — No No No No No No No No No — — No No No No — — No — — No — — — — No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes —- End Capping ) 2 0 0 0 0 0 0 — — — — — — — — — — — — — — — — — — — — — 3.8 3.9 4.2 4.0 3.5 4.2 4.2 4.1 2.5 5.3 N/A N/A N/A N/A 2.69 3.27 1.41 1.72 2.72 2.49 2.06 1.73 4 eq/g 5 meq/g 5 meq/g 0.19 meq/g 0.17 meq/g Coverage (μmole/m Calculated* Bonded Phase Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric % 1meq/g 1meq/g 0 0 0 2 0 0 0 2 3 0 0 9 4 7, Carbon Load 1 8 13 18 1 1 11 11 1 4 12 15 — — — — — — — — — — — — 16, 11, 20, 13, 25, 4.5 4.2 8.3 7.5 6.5 6.5 6.5 21.4 11.5 21.5 12.5 17.5 /g) 2 — — — — — — — — — — — — — — — — 350 350 415 340 350 300 350 360 350 300 350 350 340 200 350 300 500 350 300 200 200 200 340 100 420 650 300 100 350 550 100 100 300 420 350 350 350 350 (m urface Area Surface — — — — — — — — — — — — — — — — — 0.9 1.0 1.0 0.9 1.0 1.0 0.9 1.0 1.0 1.0 0.9 0.8 1.0 0.8 0.7 0.9 0.8 0.8 1.0 0.8 1.0 1.0 1.0 1.0 1.25 1.25 1.25 1.25 1.25 0.65 1.25 0.65 0.65 0.65 1.25 0.95 1.25 (mL/g) ore Volume P 60 80 60 60 50 — — — — — (Å) N/A N/A N/A 110 100 100 100 100 130 100 100 100 110 300 100 100 100 130 120 100 110 300 120 120 120 130 300 100 110 300 100 300 100 100 100 100 100 100 100 100 100 100 100 100 ore Size P (μm) Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 7 Spher. Spher. 7 Spher. Spher. 7 Spher. Spher. 9 Spher. 5 Spher. Spher. 10 Spher. Monolithic Monolithic Monolithic Spher. 3, 5 3, Spher. Spher. 4, 5 4, Spher. Spher. 3, 7 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Irreg. 5, 10 5, Irreg. Irreg. 5, 10 5, Irreg. Irreg. 5, 10 5, Irreg. Irreg. 5, 10 5, Irreg. Spher. 3,10 Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 3, 5, 7 5, 3, Spher. Spher. 10, 15 10, Spher. Spher. 3, 5,10 3, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 4, 5, 10 5, 4, Spher. Spher. 5, 7, 10 7, 5, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. article Shape/Size article Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. P
2 2 2
acking Material Chromolith RP-18 EC Nucleodur Gravity C18 Nucleodur P PRP-1 LiChrospher Diol LiChrospher RP-8e Nucleodur Gravity C8 Nucleodur PRP-3 Nucleosil SA Nucleosil LiChrosorb DIOL Purospher LiChrospher RP-sel B LiChrospher RP-18e Chromolith RP-8 EC Nucleosil SB Nucleosil LiChrospher CN Nucleodur Pyramid C18 Nucleodur PRP X-100 Nucleosil C4 Nucleosil Nucleosil Si Nucleosil LiChrospher Si 60 LiChrospher NH Chromolith Si PRP X-200 Nucleosil Si Nucleosil Nucleosil C18 Nucleosil Nucleosil C8 Nucleosil Nucleodur CN Nucleodur Nucleosil C4 Nucleosil LiChrospher RP-8 LiChrospher Si 100 LiChrosorb RP-sel B PRP X-300 Nucleosil C8 Nucleosil LiChrospher RP-18 LiChrosorb CN Nucleodur C18 ec Nucleodur PRP X-400 Nucleosil C18 Nucleosil LiChrosorb NH PRP X-500 Nautilus C18 Protect l PRP X-600 C18 HD Nucleosil HC-40 C8 HD Nucleosil HC-75 AB C18 Nucleosil Nucleosil Si Nucleosil Nucleosil 100 Si Nucleosil Nucleosil C8 Nucleosil Nucleosil C18 Nucleosil Nucleosil CN Nucleosil Nucleosil NH Nucleosil armstadt, Germany Darmstadt, Merck KGaA, Macherey-Nagel Other Sorbents (cont'd) Hamilton This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed. accuracy of data is not a complete list and the products, nor in terms of their is, neither in terms of manufacturers This selection HPLC Material Sorbent Characteristics Sorbent Material HPLC a selection of a selection Phenomenex 388
Appendices | HPLC Material Sorbent Characteristics HPLC MaterialSorbentCharacteristics a selectionof Supelco Restek P Other Sorbents(cont'd) This selectionis,neitherintermsofmanufacturers norintermsoftheirproducts, acompletelistandtheaccuracyofdataisnotguaranteed. Discovery Bio Discovery Wide Pore C18 Discovery HSPEG Discovery Discovery HSF5 Discovery Discovery CN Discovery Discovery C8 Discovery Discovery RP-Amide(C16) Discovery Discovery HSC18 Discovery Discovery C18 Discovery Pinnacle ll Amino Pinnacle llCyano Suplex pKB-100 Supelcosil LC-NH Pinnacle llPhenyl Supelcosil LC-CN Pinnacle llC8 Supelcosil LC-3DP Pinnacle llC18 Supelcosil LC-DP Ultra PFP Supelcosil LC-304 Supelcosil LC-18-T Ultra Amino Ultra Cyano Supelcosil LC-308 Supelcosil LC-18-S Ultra Phenyl Supelcosil LC-8-DB Ultra C4 Supelcosil LC-318 Supelcosil LC-18-DB Ultra C8 Supelcosil LC-PAH Ascentis RP-Amide Ultra Aqueous C18 PL-SCX 1000 Ascentis C18 Ultra C18 Discovery Bio Discovery Wide Pore C5 Supelcosil LC-SCX PL-SAX 1000 Allure PFPPropyl PLRP-S 300 ABZ+plus Allure Basix Discovery Bio Discovery Wide Pore C8 Supelcosil LC-SAX1 Allure Acidix ABZ Allure C18 PLRP-S 100 P olymer Labs acking Material 2 P article Shape/ize Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 5, 8, 10 Spher. 8, 10 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 5, 8 Spher. 3, 5 Spher. 3, 5 Spher. 5, 8 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 Spher. 5 (μm) P ore Size 1000 1000 120 120 180 180 180 120 180 110 120 110 110 300 120 110 120 110 300 100 120 100 300 120 100 100 300 120 100 120 100 120 100 100 300 120 100 100 120 300 120 300 120 100 300 120 (Å) 60 60 60 60 P ore Volume (mL/g) 1.0 1.0 1.0 1.0 0.6 0.5 0.6 0.6 0.5 0.6 0.5 0.6 0.5 0.6 0.6 0.6 0.6 0.6 0.6 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — Surface Area (m 300 300 200 200 200 300 200 170 170 170 170 170 170 170 170 450 100 170 450 170 100 170 170 550 100 170 — — — — — — — — — — — — — — — — — — — — 75 75 75 75 2 /g) Carbon Load 12.5 12.3 19.5 4.5 7.5 2.7 3.5 3.5 9.2 12 12 — — — — — 11 20 12 13 11 10 11 12 25 20 12 17 12 12 27 % 2 4 6 4 4 7 6 7 2 8 6 6 9 0 5 0 9 0 3 Calculated* BondedPhase overage (μmole/m Coverage 3.8 3.5 3.4 2.6 3.8 3.0 3.6 3.5 2.4 5.2 4.1 3.6 3.2 3.1 3.4 3.4 5.1 — — — — — — — — — — — — — — — — — — — — — — — — — — — 4 4 4 0 0 4 2 ) Phenomenex End Capping Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No — — — No — — — — — No 0 0 HPLC Material Sorbent Characteristics | Appendices 389 No — — — No No — — — No No No — Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes, polar Yes, End Capping ) 2 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — 2.5 2.5 3.6 3.6 3.2 3.5 4.8 3.2 2.51 2.52 1.08 2.05 5.29 1.56 3.85 3.85 2.84 5.24 2.40 2.40 3.55 3.55 Coverage (μmole/m Calculated* Bonded Phase Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric Monomeric Monomeric Polymeric Monomeric Monomeric Polymeric Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric % 6 3 7 0.3 meq/g 0.3 meq/g 7 6 7 5 9 5 4 5, Carbon Load 12 10 11 13 12 13 20 12 12 — — — — — — — — — — — — 15, 15, 22, 22, 4.0, 5.0, 7.0, 1.9, 2.5, 2.5, 6.5, 7.0, 6.5, 2.0, 3.3, 5.0, 5.0, 4.0, 4.0, 100 16.0 10.0 11.0, 13.5, 10.0, /g) 2 80 80 — — — — — — — — — — — 220 220 220 170 170 170 170 170 180 320 220 170 170 220 180 320 170 170 170 180 170 180 170 120 180 220 300 180 170 170 300 170 300 170 200 310 200 200 200 200 200 310 310 100 100 100 (m urface Area Surface - — — — — — — — — — — — — — — — — — — — — — — — — 0.5 0.5 0.5 0.7 1.0 1.0 0.7 0.7 0.5 1.0 1.0 0.7 0.7 0.7 1.0 0.7 0.6 1.0 0.7 0.6 1.0 0.7 1.0 0.7 0.7 0.7 0.7 0.7 0.7 1.0 0.65 0.65 0.65 0.65 (mL/g) ore Volume P 80 80 80 80 80 80 80 80 80 — — (Å) 130 130 130 130 120 180 250 100 175 120 120 180 100 120 120 120 180 120 300 180 120 300 250 180 100 180 120 120 100 120 100 120 190 100 150 150 150 150 150 100 100 300 300 300 100 1000 1000 1000 ore Size P (μm) Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 5 Spher. Spher. 10 Spher. Spher. 10 Spher. Spher. 10 Spher. Spher. 10 Spher. Spher. 3, 5 3, Spher. 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 5, 7 5, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 3, 5, 7 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 3, 5, 10 5, 3, Spher. Spher. 5, 10, 20 10, 5, Spher. Spher. 5, 10, 20 10, 5, Spher. Spher. 5, 10, 20 10, 5, Spher. Spher. 8, 12, 15 12, 8, Spher. Spher. 8, 12, 15 12, 8, Spher. Spher. 8, 12, 15 12, 8, Spher. article Shape/Size article P
s m (APS-2) 2
2 Phenyl-5PW ® acking Material MetaSil ODS MetaSil Phenyl AQ MetaSil MetaSil C8 TSKgel P Hypersil GOLD C18 Hypersil C1 (SAS) Hypersil BDS CN Hypersil BDS Phenyl Hypersil BDS C18 Hypersil BDS C8 Hypersil SAX TSKgel DEAE-5PW Si-A Polaris TSKgel ODS-80T TSKgel ODS-120T MetaSil Basic Hypersil C8 (MOS) Hypersil Green PAH TSKgel SP-5PW Taxsil Pursuit C18 TSKgel ODS-120A Polaris C18-A Polaris Hypersil C8 (MOS-2) Hypersil Green ENV TSKgel TMS-250 TSKgel Hypersil C4 (Butyl) Pursuit C8 TSKgel ODS-80T Polaris C8-A Polaris Hypersil C18 (ODS) Hypersil C8 (Octyl) Polaris Amide C18 Polaris Hypersil (ODS-2) Hypercarb HyperPrep HS Silica Polaris C8-Ether Polaris Hypersil Phenyl HyperPrep HS C8 Polaris NH Polaris Hypersil Phenyl-2 Hypersil CN (CPS) HyperPrep HS C18 Hypersil CN (CPS-2) HyPURITY C18 Hypersil NH Aquasil C18 BetaBasic C18 BetaBasic C8 BetaBasic C4 BetaBasic Phenyl BetaBasic CN Betasil C18 Betasil C8 BioBasic C18 BioBasic C5 BioBasic C4 Prism RP Varian Tosoh Corporation Tosoh Other Sorbents (cont'd) Thermo Fisher Scientific Inc. This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed. accuracy of data is not a complete list and the products, nor in terms of their is, neither in terms of manufacturers This selection Material Sorbent Characteristics Sorbent Material Hplc a selection of a selection Phenomenex 390
Appendices | HPLC Material Sorbent Characteristics Hplc MaterialSorbentCharacteristics a selectionof Waters Other Sorbents(cont'd) This selectionis,neitherintermsofmanufacturers norintermsoftheirproducts, acompletelistandtheaccuracyofdataisnotguaranteed. Sunfire C8 Sunfire C18 μBondapak NH μBondapak Spherisorb SAX, SAX μBondapak CN μBondapak XTerra RP8 Spherisorb OD/CN μBondapak Phenyl μBondapak Spherisorb Phenyl μBondapak C18 μBondapak XTerra RP18 Spherisorb Amino Spherisorb Nitrile Atlantis HILICSilica XBridge BEH300C18 Spherisorb C1 Spherisorb C6 Atlantis dC18 XBridge BEH130C18 Spherisorb C8 Spherisorb ODSB Atlantis T3 C18 XBridge C6Phenyl Spherisorb ODS Spherisorb ODS2 ACQUITY UPLCHSS T3 C18 XBridge ShieldRP18 Spherisorb Silica Resolve Silica Resolve CN nanoACQUITY BEH300C18 XBridge C8 Resolve C8 Resolve C18 XBridge C18 Porasil Silica nanoACQUITY BEH130C18 Symmetry300 C4 Symmetry300 μPorasil Silica Nova-Pak Silica Symmetry300 C18 Symmetry300 Nova-Pak CNHP ACQUITY UPLCBEHHILIC Nova-Pak Phenyl SymmetryPrep C8 SymmetryPrep Nova-Pak C8 Nova-Pak C18 ACQUITY UPLCBEHPhenyl Delta-Pak C18 SymmetryPrep C18 SymmetryPrep Delta-Pak C4 ACQUITY UPLCBEHShieldRP18 Delta-Pak C18 Symmetry ShieldRP18 Symmetry Delta-Pak C4 ACQUITY UPLCBEHC8 Symmetry ShieldRP8 Symmetry Bondapak C18 Bondapak Symmetry C8 Symmetry Bondapak C8 Bondapak ACQUITY UPLCBEHC18 Symmetry C18 Symmetry Bondapak C18 Bondapak Sunfire Silica AccQ_Tag UltraC18 P acking Material 2 Spher. 2.5, 3.5, 5, 10 Spher. 2.5, 3.5, 5, 10 Spher. 2.5, 3.5, 5, 10 P Spher. 2.5, 3.5, 5 Spher. 2.5, 3.5, 5 Spher. 2.5, 3.5, 5 article Shape/ize Spher. 3.5, 5, 10 Spher. 3.5, 5, 10 Spher. 3.5, 5, 10 Spher. 3.5, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3,5,10 Spher. 3,5,10 Spher. 3.5, 5 Spher. 3.5, 5 Spher. 3.5, 5 Spher. 3.5, 5 Spher. 3.5, 5 Spher. 5, 10 Spher. 5, 10 Spher. 5, 10 Irreg. 15-20 Spher. 5, 15 Spher. 5, 15 Spher. 5, 15 Spher. 5, 15 Irreg. 15-20 Irreg. 15-20 Irreg. 15-20 Spher. 5, 10 Spher. 3,5 Spher. 1.8 Spher. 1.7 Spher. 1.7 Spher. 1.7 Spher. 1.7 Spher. 1.7 Spher. 1.7 Spher. 1.7 Spher. 4, 6 Spher. 4, 6 Spher. 1.7 Spher. 10 Irreg. 10 Irreg. 10 Irreg. 10 Irreg. 10 Irreg. 10 Spher. 5 Spher. 5 Spher. 5 Spher. 4 Spher. 4 Spher. 7 Spher. 4 Spher. 7 Spher. 5 (μm) P ore Size 100 100 125 125 125 125 125 100 300 100 135 100 135 100 135 300 135 135 135 125 300 125 135 300 100 135 100 300 135 300 100 100 135 100 100 300 135 100 300 100 125 135 100 125 (Å) 80 80 80 80 80 80 80 80 80 80 80 80 90 90 90 90 60 60 60 60 60 P ore Volume (mL/g) 0.9 0.9 0.7 0.5 1.0 0.5 1.0 0.7 0.5 1.0 0.5 1.0 0.5 0.5 1.0 0.7 0.5 0.5 1.0 0.7 0.5 0.5 0.7 0.7 0.5 0.5 0.7 0.5 0.5 0.5 0.7 0.7 0.5 1.0 0.8 1.0 0.7 0.8 0.3 0.3 0.9 0.3 0.7 0.3 0.9 1.0 0.3 0.7 1.0 0.9 1.0 0.7 1.0 0.9 1.0 0.7 0.9 1.0 0.9 1.0 0.7 0.9 1.0 — — Surface Area (m 340 175 200 330 200 330 175 200 330 200 330 200 200 330 185 200 200 330 185 200 200 230 185 200 200 185 200 200 200 185 185 200 330 110 330 185 110 120 120 335 120 185 120 335 125 120 185 125 335 300 185 300 335 100 185 335 100 335 330 185 340 330 340 — — 2 /g) Carbon Load 13.5 15.0 17.5 11.5 17.5 11.5 10.2 11.7 19.1 17.0 17.0 15.0 11.7 19.1 10.0 11.5 4.0 6.0 5.0 9.3 2.5 9.8 1.9 3.1 2.2 4.7 5.8 6.2 3.0 5.1 2.8 8.5 3.0 4.6 4.0 6.8 7.3 2.6 n/a 7.3 3.5 6.0 4.0 — — — — — — — 16 12 — 14 15 11 13 17 18 15 17 13 17 17 % 0 Calculated* BondedPhase overage (μmole/m Coverage 1.15 1.72 2.64 3.29 2.97 3.36 3.12 2.98 1.49 2.98 Yes 3.0 3.3 3.2 3.1 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — 2 ) Phenomenex End Capping Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes n/a n/a n/a n/a n/a n/a n/a n/a No No No — No No No — No No No — No HPLC Material Sorbent Characteristics | Appendices 391 No Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes, polar Yes, Yes, polar Yes, Yes, polar Yes, 3 x 104 6 x 105 4 x 106 1 x 107 1 x 103 4 x 106 1 x 104 3 x 104 6 x 105 4 x 106 1 x 107 2 x 107 7 x 104 4 x 105 4 x 106 2 x 107 1 x 104 2 x 107 2 x 104 2 x 107 5 x 107 2 x 108 2 x 108 2 x 104 6 x 104 4 x 105 4 x 106 4 x 107 4 x 108 4 x 108 Limit*** Exclusion 1.5 x 103 End Capping ) 2 40 (Å) N/A N/A N/A N/A N/A N/A N/A N/A N/A 104 105 106 103 250 500 104 105 106 107 1500 100Å 500Å Mixed Mixed Mixed Mixed 1 x 103 1 x 104 1 x 105 1 x 106 ore Size** P — — — — — — — — — — — — — — — — — — — Coverage (μmole/m Calculated* Bonded Phase (μm) Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. 7 Spher. 7 Spher. 7 Spher. 7 Spher. Spher. 7 Spher. Spher. 10 Spher. 10 Spher. 10 Spher. 10 Spher. 10 Spher. 10 Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 13 5, Spher. Spher. 14, 17 14, Spher. Spher. 14, 17 14, Spher. Spher. 14, 17 14, Spher. Spher. 14, 17 14, Spher. article Shape/Size article P Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric %
9, 7, 7, 7, /g. Carbon Load 12 22, 14, 17, 12, 17, 17, 14, 2 10, 16, 10, 22, 15.5 12.0 12.0 1000H /g) ® 2 — — 175 175 510 175 510 510 335 175 100 335 335 300 200 335 335 335 300 (m acking Material urface Area Surface Ultrastyragel 104 Å Ultrastyragel 104 Å Ultrastyragel 105 Å Ultrastyragel 106 Ultrastyragel Linear Ultrastyragel 103 Å Ultrastyragel 103 P Showa Denko (cont'd) KF-806M TSKgel TSKgel 2000H μStyragel 100 Å μStyragel 100 Å μStyragel 500 Å μStyragel 1 x 103 Å μStyragel 1 x 104 Å μStyragel 1 x 105 Å μStyragel 1 x 106 HT-803 HT-804 HT-805 HT-806 HT-806M TSKgel 2500H UT-802.5 UT-806M UT-806MS UT-807 UT-807 TSKgel 3000H TSKgel 4000H TSKgel 5000H TSKgel 6000H TSKgel 7000H TSKgel GMH6 Non-Aqueous SEC/GPC Materials (cont'd) Waters Tosoh Corporation Tosoh — — — — — — 0.7 0.7 0.7 1.1 1.0 0.9 1.1 1.1 1.0 1.1 1.1 1.1 1.1 (mL/g) ore Volume P 3 x 103 6 x 103 1 x 104 7 x 104 5 x 105 1 x 106 1 x 107 5 x 103 2 x 104 7 x 104 4 x 105 4 x 106 2 x 107 2 x 108 1 x 103 1 x 107 4 x 103 1 x 105 3 x 104 5 x 105 6 x 104 1 x 107 2 x 104 2 x 107 2 x 108 6 x 105 6 x 106 1 x 107 6 x 107 Limit*** Exclusion 1.5 x 103 80 80 80 80 (Å) 125 120 125 125 120 200 300 120 120 120 200 120 120 120 120 ore Size P 50 50 50 (Å) 100 500 103 104 105 106 100 500 103 104 105 106 107 100 500 103 103 106 107 104 105 106 Mixed Mixed Mixed Mixed Mixed ore Size** P (μm) is the number of carbon atoms in the bonded silane molecule, is the number of carbon atoms in the bonded silane molecule, c Spher. 4 Spher. Spher. 4 Spher. Spher. 4 Spher. Spher. 5 Spher. Spher. 5 Spher. 1 S Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3, 5 3, Spher. Spher. 3.5, 5 3.5, Spher. Spher. 3, 5, 10 5, 3, Spher. article Shape/Size article Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 7, 10 7, 5, 3, Spher. Spher. 3, 5, 10, 15 10, 5, 3, Spher. Spher. 3, 5, 10, 15 10, 5, 3, Spher. P Spher. 2.5, 3.5, 5, 10 5, 3.5, 2.5, Spher. Spher. 2.5, 3.5, 5, 10 5, 3.5, 2.5, Spher. (μm) Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. N/A Spher. N/A Spher. N/A Spher. (M-1) Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. Spher. 5, 10 5, Spher. c c P Spher. 5, 10, 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 20 10, 5, Spher. 6 article Shape/Size article - P P c 10 1200n
) = 2
= percent carbon of bonded phase, n = percent carbon of bonded phase, c acking Material acking Material olymer Labs KF-801 PL-Gel 50 Å PL-Gel 50 P Å Phenogel 50 Å Phenogel 100 Å Phenogel 500 Å Phenogel 103 Å Phenogel 104 Å Phenogel 105 Å Phenogel 106 Phenogel Linear KF-802 KF-802.5 KF-803 KF-804 KF-805 KF-806 KF-807 P MS C18 XTerra Hydrosphere C18 PL-Gel 100 Å PL-Gel 100 Phenogel MXL XTerra MS C8 XTerra J'sphere ODS-H80 PL-Gel 500 Å PL-Gel 500 Phenogel MXM XTerra Phenyl XTerra PL-Gel 103 Å PL-Gel 103 J'sphere ODS-M80 Phenogel MXH PL-Gel 104 Å PL-Gel 104 UT-103 Å UT-103 Å UT-106 Å UT-107 PL-Gel 105 Å PL-Gel 105 J'sphere ODS-L80 PL-Gel 106 Å PL-Gel 106 PL-Gel Mixed ODS-A ODS-A ODS-A ODS-AL ODS-AM ODS-AQ ODS-AQ Pro C18 Pro C8 Pro C4 Pro C18 RS YMC-Basic where N(μmol/m M is the molecular weight of the bonded silane molecule, and S is the specific surface area of the bonded silica in m and S is the specific surface area of the bonded M is the molecular weight of the bonded silane molecule, *As per Sander, L.C., and Wise, S.A., Anal. Chem. 1984, 56, 504-510, 56, 1984, Chem. Anal. S.A., Wise, and L.C., *As per Sander, Showa Denko P Phenomenex Non-Aqueous SEC/GPC Materials Waters (continued) Waters YMC This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed. accuracy of data is not a complete list and the products, nor in terms of their is, neither in terms of manufacturers This selection (cont'd) Other Sorbents NOTE: Phenomenex has not verified above values experimentally, and does not guarantee their accuracy. Above specifications without prior notice. subject to change and does not guarantee their accuracy. Phenomenex has not verified above values experimentally, NOTE: and P HPLC Material Sorbent Characteristics Sorbent Material HPLC a selection of a selection Phenomenex 392
Appendices | HPLC Material Sorbent Characteristics HPLC MaterialSorbentCharacteristics a selectionof speaking however, theexclusionlimitismore accurately areflection ofthehydrodynamic volumeoccupied bythesolvatedcompound. ***Exclusion Limitisexpressed indaltons(themolecularweight)ofthespecifiedcompound excludedfrom thepores ofthebasematerial.Practically surface oftheparticle.RefertoTable 1foracross reference ofpore sizevalueswithseparatedmolecularweightrange. weight ofcompoundsthatcanbeseparatedonagivenSECpacking;thesevalues donotindicatetheactualsize(diameter)ofpores onthe P Table 1. **Pore Sizeisexpressed inAngstroms (10 Showa Denko P Phenomenex Aqueous SEC/GFCMaterials This selectionis,neitherintermsofmanufacturers norintermsoftheirproducts, acompletelistandtheaccuracyofdataisnotguaranteed. PolySep-GFC-P Linear PolySep-GFC-P 6000 PolySep-GFC-P 5000 PolySep-GFC-P 4000 PolySep-GFC-P 3000 PolySep-GFC-P 2000 10 10 10 10 500 100 50 P OHpak Q-802 OHpak Q-801 OHpak SB-80M OHpak SB-806 OHpak SB-805 OHpak SB-804 OHpak SB-803 OHpak SB-802.5 OHpak SB-802 OH60 PL aquagel OH50 PL aquagel OH40 PL aquagel BioSep-SEC-S 2000 Material P PolySep-GFC-P 1000 BioSep-SEC-S 4000 BioSep-SEC-S 3000 olymer Labs ore Sizevs. SeparatedMolecularWeight Range ore Size(Å) acking 6 5 4 3 sample concentrationasgiveninTable 2. temperature tobetween40and80°C,ormore reliably, reduce the viscosity, there are twopossibleoptions:Eitherincrease therunning result toward alowermolecularweight.To minimizethe effects of weight, itsretention timeinSECwillincrease tobiasthecalculated Note: Astheviscosityofapolymerincreases withmolecular MW Range(daltons) 20,000-300,000 5000-40,000 100,000-10 500-10,000 50-1000 10 1-100 6 -10 Shape/ize Spher. 10 Spher. 10 Spher. 10 7 Spher. 5 Spher. 5 Spher. 5 P Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. Spher. article (μm) 6 -10 polymers (very highMW) polymers (very highMW) polymers (very Compound lassification polymers (mediumMW) Size** Mixed monomers (smallMW) P 145 500 290 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A meters).Thisisactuallyaconventionusedbymanufacturers toindicatetheapproximate molecular polymers (smallMW) (Å) polymers (highMW) ore small polymers 10 x107(PEG) 10 x106(PEG) 20 x105(PEG) 20 x104(PEG) 50 x103(PEG) 9 x103(PEG) 2 x103(PEG) 1.8 x103 Exclusion Limit*** 5 x103 2 x107 2 x107 4 x106 4 x105 1 x105 1 x104 4 x103 2 x107 1 x106 1 x105 3 x105 1 x106 7 x105 Tosoh Corporation Compensate forViscosityEffect Table 2. DilutionofP Waters Aqueous SEC/GFCMaterials(cont'd) TSKgel G-OLIGO-PW TSKgel G-DNA-PW TSKgel GMPW TSKgel PW6000 TSKgel PW5000 TSKgel PW4000 TSKgel PW3000 TSKgel PW2500 TSKgel PW2000 TSKgel G4000SWXL TSKgel G3000SWXL TSKgel G2000SWXL TSKgel SuperSW3000 TSKgel SuperSW2000 TSKgel SW4000 TSKgel SW3000 TSKgel SW2000 Material P >2.5 M 500 Kto2.5M 50-500 K 50 K Molecular Weight (daltons) Ultrahydrogel DP Ultrahydrogel Linear Ultrahydrogel 2000 Ultrahydrogel 1000 Ultrahydrogel 500 Ultrahydrogel 250 Ultrahydrogel 120 acking Spher. 13, 17 Spher. 13, 25 Spher. 10, 17 Spher. 10, 13 Spher. 6, 13 Spher. 6, 13 Spher. 8-10 Spher. 8-10 Spher. 8-10 Spher. 8-10 Spher. 8-10 Spher. 8-10 Spher. 8-10 Shape/ize Sample Concentration(percent) olymer Sampleto Spher. 10 Spher. 10 Spher. 10 Spher. 10 Spher. 10 Spher. 6 Spher. 8 Spher. 5 Spher. 5 Spher. 4 Spher. 4 P article (μm) 0.01 0.05 0.25 0.1 >1000 Size** Mixed Blend <200 4000 1000 2000 1000 P 125 500 200 125 450 250 125 250 125 450 240 130 120 500 250 120 (Å) ore 1.5 x105(Protein) 1.5 x105(Protein) 7 x106(Protein) 5 x105(Protein) 5 x105(Protein) 7 x106(Protein) 5 x105(Protein) 1 x105(Protein) Phenomenex 3 x103(PEO) 5 x103(PEO) 7 x106(PEO) 7 x106(PEO) 1 x106(PEO) 4 x105(PEO) 8 x104(PEO) 5 x103(PEO) 5 x107(PEG) 8 x106(PEG) 1 x106(PEG) 3 x105(PEG) 5 x104(PEG) 2 x103(PEG) 8 x106(PEG) 3 x103(PEG) Exclusion Limit***