Sustainable Conservation of Endangered Alpine Medicinal and Aromatic Plant Nardostachys Grandiflora DC. Through Top Edge Cutting
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International Journal for Environmental Rehabilitation and Conservation ISSN: 0975 6272 XI (SP2): 64 — 74 www.essence-journal.com Original Research Article Sustainable conservation of endangered alpine medicinal and aromatic plant nardostachys grandiflora DC. through top edge cuttings using plant growth regulators (PGR’s) Dobhal, Pradeep; Purohit, Vijay Kant and Chauhan, Jaidev High Altitude Plant Physiology Research Centre (HAPPRC), H.N.B. Garhwal University (A Central University), Srinagar, Garhwal, Uttarakhand Corresponding Author: [email protected] A R T I C L E I N F O Received: 4 July 2020 | Accepted: 20 August 2020 | Published Online: 30 September 2020 EOI: 10.11208/essence.20.11.SP2.128 Article is an Open Access Publication This work is licensed under Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by/4.0/) ©The Authors (2020): Publishing Rights @ MANU—ICMANU and ESSENCE—IJERC. A B S T R A C T Nardostachys grandiflora DC. (Jatamansi) is a small herbaceous species of family Caprifoliaceae (previously Valerianaceae) commonly known as Jatamansi, Indian nard or spikenard. It is a perennial, dwarf, hairy, rhizomatous medicinal and aromatic herb, grows in steep, moist, rocky, undisturbed grassy slopes in higher Himalayan region. The causative aim of our designed study was preparative and conservative point of view, prior producing the large number of plants using the top edge cuttings obtained from plants at uprising senescence and investigating the effect on N. grandiflora, of different concentrations were tried to stimulate sprouting and rooting of IAA, IBA NAA and BAP respectively. The cuttings were dipped in different concentration of PGRs for 24 hours and planted in Styrofoam trays at Greenhouse condition (21.5 ± 3.30°C while night temperature was 8.60 ± 2.45 °C and the relative humidity was between 65-80% during the experiment) containing mixture of Soil, Sand, Farm Yard Manure (FYM), and Forest Litter (FL) at the ratio of 1:1:1:1. The study reveals that IBA (50 µM) showed better result compared to other PGRs. Experiments indicated that the large number of N. grandiflora plants can be obtained through using top edge cuttings (upper part of shoot portion) without damaging the plants before senescence period. K E Y W O R D S Nardostachys grandiflora DC | Top edge cuttings | Vegetative propagation | Plant Growth Regulators | Conservation C I T A T I O N Dobhal, Pradeep; Purohit, Vijay Kant and Chauhan, Jaidev (2020): Sustainable Conservation of Endangered Alpine Medicinal and Aromatic Plant Nardostachys grandiflora DC. Through Top Edge Cuttings using plant growth regulators (PGR’s). ESSENCE Int. J. Env. Rehab. Conserv. XI (SP2): 64 — 74. https://eoi.citefactor.org/10.11208/essence.20.11.SP2.128 64 ESSENCE—IJERC | Dobhal et al. (2020) | XI (SP2): 64 — 74 Introduction of rhizomes for medicinal and aromatic use, habitat degradation and other biotic Nardostachys grandiflora DC. One of the interferences, the species has become highly economical species commonly known critically endangered depending on habitats as jatamansi. It is a perennial rhizomatous (Airi et al., 2000; Nautiyal et al., 2003). medicinal and aromatic herb growing in Hence emphasis was given on its subalpine/alpine areas of the north western conservation and multiplication by several Himalayan region of Pakistan, India, Nepal, workers (Chauhan and Nautiyal, 2005; China and Tibet, mostly grows in immerse, Chauhan et al., 2008). Effective conservation, moist, stony, slopes between 3000-5000 m asl management and recovery of rare and in higher Himalayan region. It is traditionally endangered species can be deliberated used for its incense, perfume and in ethno through variability analysis (Chauhan et al., medicine as well as modern medicine 2011). A single plant species generally (Chauhan and Nautiyal, 2005). In different consists of genetically differentiated parts of India. The species has been declared populations that are outcomes of the as ‘Endangered’ in Arunachal Pradesh, interactions among natural selection, gene Sikkim and Himachal Pradesh, ‘Critically flow and genetic drift (Suichi, 2003). There Endangered’ in Uttarakhand and Kashmir are several reports available on distribution district, (Kaul, 2001) and ‘Vulnerable’ in pattern, medicinal properties (Purohit et al., Nepal during Conservation Assessment and 2017), etc. The Governments is making Management Plan (CAMP) workshop held in serious effort to promote conservation and the year 2001 (Bhattarai et al., 2002). It is cultivation of this species through different listed in CITES (Convention on International promotional schemes. In this context, effect Trade in Endangered Species of Wild Flora of various growth regulators was assessed for and Fauna) appendix II and the Red Data effective propagation of this species. Book of Indian Plants (Nayar and Sastry, Vegetative propagation assists in sustaining 1988; Mulliken, 2000). It is widely used for genetic identity of medicinal plants and safe the hair related problems like hair loss, guarding the populations from destruction and greyness, growth and nervous system deterioration (Kharkwal et al., 2008). Plant disorders i.e., insomnia, epilepsy, learning growth regulators are known their role in and memory disorders (Yadav et al., 2011; growth and other lively activities in plants. Rahman et al., 2011; Singh et al., 2013). In Auxins are the group of hormones (IBA, IAA, Ayurveda it is used for cure various elements NAA etc), which stimulates the sprouting and related disorder as Medhya (brain tonic), rooting in cuttings, promotes cell elongation Rasayana (Rejuvenative to the mind), and growth by synthesized in young shoot tips Nidrajnana (promotes sleep) and and transported to different tissues and are Manasrogaghna (alleviates mental diseases) widely used in vegetative propagation of (Sharma et al., 2001). It is also useful in plants. Whereas, cytokinins play an important antibacterial, antifungal, nematicidal, anti- role in cell division. In this experiment auxins malarial and mosquito repellent activity group of hormones (IBA, IAA and NAA) and (Singh et al., 2013). Due to over-exploitation 65 ESSENCE—IJERC | Dobhal et al. (2020) | XI (SP2): 64 — 74 BAP were used and stock solutions (mg/mL) the cuttings were treated with different of Indole-3-acetic acid (M.W. 175.2), Indole- concentrations of PGRs i.e. IAA, IBA NAA 3-butyric acid (M.W. 203.2), and and BAP (50, 100, 150 and 200 µM) for 24 Naphthalene acetic acid (M.W. 186.2) and hours treated cuttings were planted in Benzylaminopurine (M.W. 225.3) were Styrofoam trays containing mixture of soil, prepared. Hormone solutions such as 50 µM sand, FYM, and FL at the ratio of 1:1:1:1. All 100 µM, 150 µM and 200 µM were prepared the Styrofoam trays containing cuttings were from the stock solutions. In this context, effect kept inside the greenhouse condition at of various growth regulators was assessed on Srinagar (550 m asl) for further observations. vegetative propagation for effective Recording of cuttings parameters conservation of this species. Statistical analysis Material and Methods Data analysis: One-way ANOVA was Plant materials performed to find out the variance among the Top edge cuttings of N. grandiflora were treatments, further Duncan Multiple Range collected from nursery of Tungnath (TN): an Test (DMRT) was applied to find out the alpine zone (3400 m asl) located between variance within groups. Regression plots were 30◦14' N latitude and 79◦13' E longitude in plotted for all PGRs, different concentrations Rudraparyag district of Garhwal, Uttarakhand of PGRs used as predictor and growth Himalaya, India. After collection top edge percentage of morphological parameters as cuttings was tested to propagate the species response variable. Growth performance was under polyhouse conditions at Srinagar calculated for number of leaves, plant height, Garhwal (550 m asl), Uttarakhand (29◦26’-31 stem diameter, leaf area and fresh biomass 28’ N and 77 49’-80 6’ E), India. using the following formula: Preparation of top edge cuttings (Last observation- First The top edge cuttings (7.11 ±1.69 cm Length; % observation) = 4.34 ±1.59 mm Diameter; 2.93±1.29gm Growth weight), prepared from five-year-old plants Last observation×100 grown in nursery at Tungnath. The top edge or above ground biomass which totally dries during winter months was used for formation First observation was taken after 30 DAP and of new plants. The top edge (twigs) collected last observations after 150 DAP. Readings after complete seed formation and collection were taken on the monthly basis (30 days’ at the level of soil were brought to Srinagar in interval). Dry weight, number of roots, length the first week of December. After that the of roots and diameter of roots were recorded application was composed of 3 replications only once so growth rate for these variables (45 number in each treatment) were washed was not calculated. carefully with tap water to remove soil particles followed by distilled water after that 66 ESSENCE—IJERC | Dobhal et al. (2020) | XI (SP2): 64 — 74 Sprouting Percentage Number of cutting survive Survival The total number of top edge cuttings that Percenta = sprouted under each treatment were counted Total number of cutting ge (%) and expressed as sprouting percentage: planted ×100 Number of cutting sprouted Sprouting percentage was taken after 30 DAP Sprouting cuttings and survivability and rooting Percentage = Total number of cutting percentage was calculated after 150 DAP. Dry (%) planted ×100 weight of cuttings was recorded after drying them in electric oven for 48 hours at 80°C and Rooting Percentage weight was taken by electronic weighing The total number of top edge cutting within balance SARTORIUS (CPA224), in the each treatment which showed the growth of laboratory. Thus eight parameters were root (≥ 1mm) were counted and expressed in determined at the end of the experiment, percentage: namely: Plant height (PH), number of leaves (NL), Leaf area (LA), stem diameter (SD), Number of cutting rooted Number of Roots (NR), Length of Roots Rooting (LR), Diameter of Roots (DR), and Dry Percentage = total number of cutting Biomass of Cuttings (DB).