Received: 26 May 2020 | Revised: 23 July 2020 | Accepted: 24 July 2020 DOI: 10.1111/jfd.13243 SHORT COMMUNICATION A comparison of the use of different swab materials for optimal diagnosis of amoebic gill disease (AGD) in Atlantic salmon (Salmo salar L.) Carolina Fernandez-Senac1 | Sophie Fridman1 | Jadwiga Sokolowska1 | Sean J. Monaghan1 | Teresa Garzon2,3 | Monica Betancor1 | Giuseppe Paladini1 | Alexandra Adams1 | James E. Bron1 1Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, UK Abstract 2Mowi Scotland, Blar Mhor Industrial Estate, Routine gill swabbing is a non-destructive sampling method used for the downstream Fort William, UK qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causa- 3PatoGen, The Moorings, Suite 7, Malin House European Marine Science Park, tive agent of amoebic gill disease (AGD). Three commercially available swabs were Dunbeg, Oban, UK compared aiming their application for timelier AGD diagnosis (Calgiswab® (calcium ® Correspondence alginate fibre-tipped), Isohelix DNA buccal and cotton wool-tipped). Calcium algi- Carolina Fernandez-Senac, Institute of nate is soluble in most sodium salts, which potentially allows the total recovery of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, biological material, hence a better extraction of target organisms’ DNA. Thus, this Scotland, UK. study consisted of (a) an in vitro assessment involving spiking of the swabs with Email:
[email protected] known amounts of amoebae and additional assessment of retrieval efficiency of Funding information amoebae from agar plates; (b) in vivo testing by swabbing of gill arches (second, third European Union’s Horizon 2020 research, Grant/Award Number: 634429 and fourth) of AGD-infected fish.