Accomplishments Report (2001-2005)
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Study Guide Entomology & Nematology Department
STUDY GUIDE ENTOMOLOGY & NEMATOLOGY DEPARTMENT DPM COMPREHENSIVE EXAMINATIONS The Entomology & Nematology Comprehensive Examinations consist of 3 sections: pest identification (30%), pest biology and management (40%), and core concepts and synthesis (30%). These examinations are limited to information about invertebrate animal pests, principally insects and nematodes, but also plant feeding mites and terrestrial molluscs. A. Pest identification Students will be presented with insects, mites, molluscs, and nematodes that they must identify. Some may be recognizable by sight, but others may require keys for identification. Students will be provided with identification aids (keys), where necessary, and be expected to use them to identify the subjects accurately. The unknowns will be selected from the list of important insect, mite, mollusc, and nematode pests (Table 1) though we will emphasize those with a single or double asterisk [* or **]), as these normally are the more important pests. Included in this list are some that pose a threat but are not currently found in Florida. B. Pest biology and management Students will answer 8-10 questions on insect, mite, mollusc, and nematode pest biology (sampling, distribution, life cycle, damage) and management. The animals for which students are responsible to know biology and management are listed in Table 1 (preceded by double asterisk [**]). C. Core Concepts and Synthesis Section: Students will answer 3 or 4 questions that cover core areas of Entomology/Nematology and demonstrate knowledge of core areas, but also analysis and problem solving. Suggested reference/reading material is listed in Table 2. You might want to read through these in preparation for the Comprehensive Examinations. -
Status of Aphanomyces Root Rot in Wisconsin
STATUS OF APHANOMYCES ROOT ROT IN WISCONSIN C.R. Grau1 Introduction Alfalfa is the primary forage crop in Wisconsin and is a key element in the state’s dairy industry. The yield of new varieties is greater than that of Vernal and other older varieties due to genetic gains made by breeders over the years. Much of the yield advantage of new varieties may be attributed to efforts to breed for resistance to a wide variety of major pathogens of alfalfa such as Verticillium, Phytophthora, and Aphanomyces. Although the yield gap has widened between new varieties and Vernal, yield of all varieties has declined steadily in Wisconsin during the past 30 years (Wiersma et al., 1997). There are several possible explanations for this situation including changing climate and the difficulties inherent in dealing with such a genetically diverse crop such as alfalfa. From a pathologist’s perspective, new disease-causing organisms or new strains of previously described pathogens may also play a role in limiting yield gains for Wisconsin alfalfa growers. At the University of Wisconsin-Madison, we are conducting research on alfalfa diseases, particularly those caused by organisms that have not been studied previously with respect to their presence and influence on the alfalfa crop. Aphanomyces root rot is one relatively new disease of alfalfa that has been studied in this regard. Overview of Aphanomyces Root Rot Although the cause, the fungus Aphanomyces euteiches, was reported to infect alfalfa in the 1930’s, this organism was considered a minor pathogen of alfalfa. In contrast, Phytophthora root rot, caused by Phytophthora medicaginis, was considered the only significant cause of root disease of alfalfa in wet soil situations. -
~~ 8Ibliografia Del Salivazo De Los Pastos Y La Superfamilia
R~P r ~~ 8IBLIOGRAFIA DEL SALIVAZO DE LOS PASTOS ?~ Y LA SUPERFAMILIA CERCOPOIDEA c,.j Junio de 2001 (671 referencias) csomu Centro Internacional de Agricultura Tropical Internacional Center for Tropical Agriculture U\l ; :--,. ¡· !(t ~ ~~ e.CIO N Y L U l'r'il.\1 ACIUN u 1 DI~. 'uu; lf uO j~ Babhografía del Sahvazo de los Pastos y la Superfamaha Cercopoadea Jun1o de 2001 (671 referencias) Compilada por Dan1el C Peck B1oecolog1a/MIP del Sahvazo Proyecto de Grammeas y Legummosas Trop1cales CIAT Cah Colomb1a Portada Zulta carbonarta Z carbonarta Mahanarva andtgena y Z pubescens(en el sent1do de las manec1llas del reloJ) Fotos por Franc1sco Lopez 1 P ALADRAS CLAVES UTILIZADAS REGION CULTIVO MANEJO BABITAT BIOLOGIA CLASSIFICACION Australia lnd11 lndoncsaa New Zealand axonomy Colombta Ecuador Pero enes S 0 ght synd ome V cnc::zuela orghum asture assessmcnt K. lrawbmy asnm: pests S tylosanthes esbc1dcs uprean hytoto enu ñgrass enmls ro In CODIGOS ASIGNADOS AOI Cerc6p1dos asocl3dos con grammeas C02 Control qWIDJco A02 Otra Cercopmdea C03 Control cultural C04 Res1stenc13 vanetal BOO BIOECOLOOIA C05 Impacto BOl Comportanuento C06 Mane¡o mtegrado de plagas B02 BIO(Ogl3 C07 Cria BOl 013pausa C08 Muestreo & Momtm:eo B04 Eco logia B05 Ománnca poblac!onal 000 HOSPEDEROS B06 Taxonoiillll & S!Stemállca 001 Grammeas fona¡eras 002 Otras grammeas coo MANEJO 003 Caña de azucar COl Control bmlógtco & EnCI1llgos naturales 004 Hospederos no-grammeas 2 Abclui-Nour U., aad L. Labo d.l9!15. RtviSlon du s=e Ph laenus Sial 1864 au Liban aV<Cia descnpuon d\me auvelle~ P mlan1 n sp (Homuplm Auchenoll'hyncha, Cen:o¡ndae) Nouveau Revue d'Entomol"SSe 12(4) 297 303 Key wprds laXonomy Cmopcndea, Phtlaenus As1a, Aphrophondae, A02 ,...-'&<1111 ~ J J J A. -
Alfalfa Seedling and Root Diseases Gary P
Integrated Crop Management News Agriculture and Natural Resources 4-8-2002 Alfalfa seedling and root diseases Gary P. Munkvold Iowa State University, [email protected] Follow this and additional works at: http://lib.dr.iastate.edu/cropnews Part of the Agricultural Science Commons, Agriculture Commons, and the Plant Pathology Commons Recommended Citation Munkvold, Gary P., "Alfalfa seedling and root diseases" (2002). Integrated Crop Management News. 1703. http://lib.dr.iastate.edu/cropnews/1703 The Iowa State University Digital Repository provides access to Integrated Crop Management News for historical purposes only. Users are hereby notified that the content may be inaccurate, out of date, incomplete and/or may not meet the needs and requirements of the user. Users should make their own assessment of the information and whether it is suitable for their intended purpose. For current information on integrated crop management from Iowa State University Extension and Outreach, please visit https://crops.extension.iastate.edu/. Alfalfa seedling and root diseases Abstract Soil conditions so far this spring are drier than normal, which means fewer problems with seedling disease in early alfalfa plantings. But things can change quickly, because the fungi causing these diseases are sensitive to surface soil moisture. With rain events around the time of planting, seedling diseases can appear rapidly. The most important fungi attacking alfalfa seedlings are Aphanomyces euteiches,Phytophthora medicaginis, and several species of Pythium. Keywords Plant Pathology Disciplines Agricultural Science | Agriculture | Plant Pathology This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/cropnews/1703 10/26/2015 Alfalfa seedling and root diseases Alfalfa seedling and root diseases Soil conditions so far this spring are drier than normal, which means fewer problems with seedling disease in early alfalfa plantings. -
Department of Plant Pathology Periodic Program Review October
Department of Plant Pathology Periodic Program Review October 2015 Self Study Department of Plant Pathology Periodic Program Review 2009–2015 Self Study Submitted to: Dean Nancy Cox College of Agriculture, Food and Environment Submitted by: Christopher L. Schardl, Chair Department of Plant Pathology September 30, 2015 Department of Plant Pathology Self-Study Report Checklist: College of Agriculture, Food and Environment Unit Self-Study Report Checklist Page Number Academic Department (Educational) Unit Overview: or NA 1 Provide the Department Mission, Vision, and Goals Pg. 7 2 Describe centrality to the institution’s mission and consistency with state’s goals: A program should adhere to the role and scope of the institution as set forth in its mission statement and as complemented by the institutions’ strategic plan. There should be a Pg. 7 clear connection between the program and the institutions, college’s and department’s missions and the state’s goals where applicable. 3 Describe any consortial relations: The SACS accreditation process mandates that we “ensure the quality of educational programs/courses offered through consortial relationships or contractual agreements and that the institution evaluates the consortial Pg. 9 relationship and/or agreement against the purpose of the institution.” List any consortium or contractual relationships your department has with other institutions as well as the mechanism for evaluating the effectiveness of these relationships. 4 Articulate primary departmental/unit strategic initiatives for the past three years and the department’s progress towards achieving the university and college/school initiatives (be Pg. 7, 35, 41 sure to reference Unit Strategic Plan, Annual Progress Report, and most recent Implementation Plan) 5 Department or unit benchmarking activities: Summary of benchmarking activities including institutions benchmarked against and comparison results: number of faculty Pg. -
Chickpea Package
chickpea INTEGRATED PEST MANAGEMENT INNOVATION LAB ICRISAT chickpea package Chickpea (Cicer arietinum L.) (Fabaceae) is an annual legume (pulse crop) of the family Fabaceae. It is commonly known as garbanzo bean, Egyptian WHAT IS IPM? pea, and gram, or Bengal gram. Based on seed color, chickpea is also Integrated pest management classified as ‘Desi’ or ‘Kabuli’ types. Desi chickpea has a pigmented (tan to (IPM), an environmentally-sound black) seed coat and small seed size (greater than 100 seeds/oz), whereas and economical approach to pest Kabuli or garbanzo bean has white to cream-colored seed coats and size control, was developed in response ranges from small to large (50–100 seeds/oz). Chickpea originated in the to pesticide misuse in the 1960s. Middle East and got domesticated in Southeast Asia. Currently, chickpea is Pesticide misuse has led to pesticide grown in about 57 countries in Asia, Australia, Middle East, North America, resistance among prevailing pests, a South America, Africa, and Europe. Major producers of Chickpea are India, resurgence of non-target pests, loss Australia, Myanmar, Ethiopia, Turkey, Russia, Pakistan, Iran, Canada, USA, of biodiversity, and environmental Mexico, Malawi, Morocco, and Syria. In 2019, India shared 70% of global and human health hazards. Lab (IPM IL) Management Innovation Pest Integrated chickpea production. The chickpea plant is a self-pollinating, small bush with a height ranging from 30 to 60 cm. Chickpea crop performs best with the long, warm growing season and is usually grown as a rainfed, cool- WHAT ARE season crop in semiarid regions. Well-draining, sandy loam soils with a pH IPM PACKAGES? 5.0–7.0, and annual rainfall of 600–1000 mm are best for this crop. -
Species of Phytophthora Associated with a Native Ecosystem in Gauteng
Species of Phytophthora associated with a native ecosystem in Gauteng by Jan Hendrik Nagel BSc Genetics (Hons) Submitted in partial fulfilment of the requirements for the degree Magister Scientiae In the Faculty of Natural & Agricultural Sciences, Department of Genetics, University of Pretoria, Pretoria September 2012 Supervisor: Dr. Marieka Gryzenhout Co-supervisors: Prof. Bernard Slippers Prof. Michael J. Wingfield i Declaration I, Jan Hendrik Nagel, declare that the thesis/dissertation, which I hereby submit for the degree Magister Scientiae at the University of Pretoria, is my own work and has not previously been submitted by me for a degree at this or any other tertiary institution. SIGNATURE: ___________________________ DATE: ________________________________ ii TABLE OF CONTENTS ACKNOWLEDGEMENTS 1 PREFACE 2 CHAPTER 1 4 DIVERSITY , SPECIES RECOGNITION AND ENVIRONMENTAL DETECTION OF Phytophthora spp. 1. INTRODUCTION 6 2. THE OOMYCETES 7 2.1. OOMYCETES VERSUS FUNGI 7 2.2. TAXONOMY OF OOMYCETES 7 2.3. DIVERSITY AND IMPACT OF OOMYCETES 9 2.4. IMPACT OF Phytophthora 11 3. SPECIES RECOGNITION IN Phytophthora 13 3.1. MORPHOLOGICAL SPECIES CONCEPT 14 3.2. BIOLOGICAL SPECIES CONCEPT 14 3.3. PHYLOGENETIC SPECIES CONCEPT 15 4. STUDYING Phytophthora IN NATIVE ECOSYSTEMS 17 4.1. ISOLATION AND CULTURE OF Phytophthora 17 4.2. MOLECULAR DETECTION AND IDENTIFICATION TECHNIQUES FOR Phytophthora 19 4.3. RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) 19 4.4. SINGLE -STRAND CONFORMATION POLYMORPHISM (SSCP) 20 4.5. PCR DETECTION WITH SPECIES -SPECIFIC PRIMERS 21 4.6. QUANTATIVE PCR 22 4.7. DNA HYBRIDIZATION BASED TECHNIQUES 23 4.8. SEROLOGICAL TECHNIQUES 24 4.9. PCR AMPLIFICATION WITH GENUS SPECIFIC PRIMERS 25 5. -
Single Plant Selection for Improving Root Rot Disease (Phytophthora Medicaginis) Resistance in Chickpeas (Cicer Arietinum L.)
Euphytica (2019) 215:91 https://doi.org/10.1007/s10681-019-2389-2 (0123456789().,-volV)(0123456789().,-volV) Single plant selection for improving root rot disease (Phytophthora medicaginis) resistance in Chickpeas (Cicer arietinum L.) John Hubert Miranda Received: 19 December 2017 / Accepted: 1 March 2019 Ó The Author(s) 2019 Abstract The root rot caused by Phytophthora results also indicated certain level of heterozygosity- medicaginis is a major disease of chickpea in induced heterogeneity, which could cause higher Australia. Grain yield loss of 50 to 70% due to the levels of susceptibility, if the selected single plants disease was noted in the farmers’ fields and in the were not screened further for the disease resistance in experimental plots, respectively. To overcome the advanced generation/s. The genetics of resistance to problem, resistant single plants were selected from the PRR disease was confirmed as quantitative in nature. National Chickpea Multi Environment Trials (NCMET)—Stage 3 (S3) of NCMET-S1 to S3, which Keywords Polygenic Á Back cross Á Disease were conducted in an artificially infected phytoph- management Á Interspecific crosses Á Disease thora screening field nursery in the Hermitage resistance Á Breeding method Á Selection technique Á Research Station, Queensland. The inheritance of Heterozygosity Á Heterogeneity resistance of these selected resistant single plants were tested in the next generation in three different trials, (1) at seedling stage in a shade house during the off- season, (2) as bulked single plants and (3) as individual Introduction single plants in the disease screening filed nursery during the next season. The results of the tests showed Chickpea is an important cash crop of Australia, that many of the selected single plants had higher level grown during winter for its grain and agronomic value. -
Molecular Detection of Phytophthora Sojae in Glycine Max Through Conventional and Real-Time PCR J
Molecular detection of Phytophthora sojae in Glycine max through conventional and real-time PCR J. C. Bienapfl, J. A. Percich, and D. K. Malvick Department of Plant Pathology, University of Minnesota, St. Paul 55108 INTRODUCTION Table 1. Isolates of Phytophthora sojae and other pathogens used to test specific primers PSOJF1- A B C PSOJR1 and results from the testing with conventional PCR (sPCR) and real-time PCR (qPCR) Phytophthora rot, caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most damaging diseases of soybean (Glycine max) in the U.S. (4). In 2005, P. sojae caused Genus and species Number of isolates sPCRa qPCRb,c an estimated yield loss of 1.1 million tons in the U.S. (7). Field diagnosis of Phytophthora Aphanomyces euteiches 1 - ND rot may be difficult due to symptoms resembling those caused by other pathogens such as Pythium or Diaporthe species. Symptoms include root rot and stem rot (Fig. 1). Methods to Diaporthe phaseolorum var. caulivora 2 - ND identify P. sojae involve direct isolation on semi-selective media. The development of a Fusarium acuminatum 2 - ND PCR-based assay for the rapid and sensitive detection of P. sojae could facilitate pathogen Fusarium equiseti 2 - ND identification and lead to more effective disease management. Fusarium graminearum 2 - ND PCR assays for rapid and specific detection of P. sojae using two sets of primers have been reported by other researchers, but they have limitations or have not been validated. PS1- Fusarium oxysporum 4 - ND PS2 were developed in China and PSOJF1-PSOJR1 were developed at Michigan State Fusarium proliferatum 2 - ND Fig. -
Biological Control of Phytophthora Root Rots on Alfalfa and Soybean with Streptomyces
Biological Control 23, 285–295 (2002) doi:10.1006/bcon.2001.1015, available online at http://www.idealibrary.com on Biological Control of Phytophthora Root Rots on Alfalfa and Soybean with Streptomyces Kun Xiao,* Linda L. Kinkel,* and Deborah A. Samac*,† *Department of Plant Pathology and †USDA-ARS Plant Science Research, University of Minnesota, St. Paul, Minnesota 55108 Received May 11, 2001; accepted November 16, 2001 sojae was the second-most important yield-suppressing A collection of 53 antibiotic-producing Streptomyces disease in the north-central region of the United isolated from soils from Minnesota, Nebraska, and States. In 1994, an estimated 560,300 metric tons of Washington were evaluated for their ability to inhibit soybean were lost to P. sojae in the United States, plant pathogenic Phytophthora medicaginis and Phy- making it third among yield-suppressing diseases tophthora sojae in vitro. Eight isolates having the (Doupnik, 1993; Wrather et al., 1997). greatest pathogen-inhibitory capabilities were subse- Phytophthora root rots are difficult to control effec- quently tested for their ability to control Phytoph- tively. A variety of methods have been studied for use thora root rots on alfalfa and soybean in sterilized vermiculite and naturally infested field soil. The in control of Phytophthora root rots of alfalfa and soy- Streptomyces isolates tested significantly reduced root bean. Plant resistance is currently the major control rot severity in alfalfa and soybean caused by P. medi- method for Phytophthora root rots on both crops (Er- caginis and P. sojae, respectively (P < 0.05). On alfalfa, win and Ribeiro, 1996). Unfortunately, none of the isolates varied in their effect on plant disease severity, currently available resistant alfalfa cultivars is im- percentage dead plants, and plant biomass in the pres- mune to the disease, especially at high pathogen inoc- ence of the pathogen. -
Anthropod Community Associated with the Webs of the Subsocial Spider Anelosimus Studiosus
Georgia Southern University Digital Commons@Georgia Southern Electronic Theses and Dissertations Graduate Studies, Jack N. Averitt College of Fall 2008 Anthropod Community Associated with the Webs of the Subsocial Spider Anelosimus Studiosus Sarah Natalie Mock Follow this and additional works at: https://digitalcommons.georgiasouthern.edu/etd Recommended Citation Mock, Sarah Natalie, "Anthropod Community Associated with the Webs of the Subsocial Spider Anelosimus Studiosus" (2008). Electronic Theses and Dissertations. 702. https://digitalcommons.georgiasouthern.edu/etd/702 This thesis (open access) is brought to you for free and open access by the Graduate Studies, Jack N. Averitt College of at Digital Commons@Georgia Southern. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Digital Commons@Georgia Southern. For more information, please contact [email protected]. THE ARTHROPOD COMMUNITY ASSOCIATED WITH THE WEBS OF THE SUBSOCIAL SPIDER ANELOSIMUS STUDIOSUS by SARAH N. MOCK (Under the Direction of Alan Harvey) ABSTRACT Anelosimus studiosus (Theridiidae) is a subsocial spider that has a diverse arthropod fauna associated with its webs. From south Georgia, I identified 1006 arthropods representing 105 species living with A. studiosus , and 40 species that were prey items from 250 webs. The arthropods seen in A. studiosus webs represented a distinct community from the arthropods on the tree. I found that Barronopsis barrowsi (Agelenidae) and Frontinella pyramitela was similar to A. studiosus in web structure and that B. barrowsi webs contained multiple arthropods. Also, previously known as asocial, B. barrowsi demonstrated sociality in having multiple adults per web. Lastly, the inquiline communities in the webs of A.studiosus and B.barronopsis contained many different feeding guilds, including herbivores, omnivores, generalist predators, kleptoparasites, and aranievores. -
Medicago Truncatula Resistance to Oomycetes
Medicago truncatula handbook version November 2006 Medicago truncatula resistance to Oomycetes Anne Moussart1, Bernard Tivoli1, Deborah Samac2, Nola D’Souza3 1 INRA/AGROCAMPUS Rennes, Unité Mixte de Recherche BiO3P, BP 35327, 35653 Le Rheu Cedex, France. 2 USDA-ARS-Plant Science Research, 1991 Upper Buford Circle, 495 Borlaug Hall, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108 U.S.A. 3 Australian Centre for Necrotrophic Fungal Pathogens (ACNFP), School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch WA 6150, Australia. Table of contents 1. Resistance to A. euteiches 2. Resistance to P. medicaginis Two main oomycetes are considered: Aphanomyces euteiches which causes root rot disease on pea, alfalfa, lentils and Phytophthora medicaginis (formerly P. megasperma f. sp. medicaginis) which causes Phytophthora root rot on alfalfa (Hansen and Maxwell, 1991), annual medic species (DeHann et al., 1996) and chickpea (Cicer arietinum), which is a more susceptible host (Irwin et al 1995). 1. Resistance to A. euteiches (Anne Moussart, Bernard Tivoli) This test published by Moussart et al. (2006) is a modified version of the test developed for the evaluation of pea resistance to A. euteiches by Moussart et al. (2001). Plant material Seeds were allowed to germinate on moist filter paper for 48 to 72 h at 25°C in the dark and were then transferred to 500 ml plastic pots containing unsterilized vermiculite (VERMEX, M). Five seeds of a single accession were sown per pot, with each pot constituting a replicate. There were four replicates per accession. Pots were arranged in a completely randomized design, in a controlled environment chamber under constant conditions (thermoperiod: 25/23°C and 16 h photoperiod).