2017 Poster Abstracts
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Abstracts for Poster Presentations Session 001: Oil Spill Response and Mitigation Agents Evaluation of the Oil Removal Efficiency of Three Shoreline Cleaner Products E. Wirth1, E. Pisarski2, B. Shaddrix2, M. Fulton1 1NOAA, Charleston, SC, 2JHT, Inc., Charleston, SC Oil spill mitigation protocols allow for responders to implement multiple technologies during spills. Surface washing agents (or shoreline cleaners, SLC) are chemicals applied in specific situations where it is desired to remove stranded oil from substrates such as shorelines, seawalls, or vegetation. There are a number of products that are classified as SLC products, yet few studies have characterized the oil removal efficiency of individual products. The objective of this study was to determine the oil removal efficiency of three SLC products (Accell Clean SWA, Cytosol™ and PES-51). The products were selected to represent different chemical constituents. Accell Clean SWA is a surface washing agent containing a combination of surfactants with non-enzymatic proteins. CytoSol is a surface washing agent derived from vegetable oil and animal fat methyl esters. PES-51 is listed as a miscellaneous oil spill control agent and consists primarily of d-Limonene. Artificial substrates in the form of ceramic tiles were selected to represent hard substrates. Multiple ceramic tiles were oiled using Louisiana Sweet Crude oil and allowed to weather in a greenhouse. A baseline measurement for the total oil mass on the tiles was determined. Shoreline cleaners were then applied to oiled tiles, allowed to soak for 30 minutes, and then rinsed with seawater. Oil removal efficiency was measured by solvent extraction (hexane and dichloromethane) of the tiles followed by quantification of Total Extractable Hydrocarbons (mg/tile) using gas chromatography mass spectrometry. Removal efficiency was determined by comparing to oiled- unwashed tiles and oiled-seawater alone tiles. The results of this study provide new data on oil removal efficiencies for three SLC products. Understanding SLC effectiveness is an important facet of oil spill response and results from this study will assist response managers in making environmentally sensitive decisions. Water Oil Spills Cleanup Using Oleophilized Peanut Hulls as Sorbent H. Solis Envigeo, Montreal, QC, Canada The suggested remediation process is based on the utilization of Oleophilized Peanut Hulls (OPH 28) as an organic sorbent. A natural biodegradable organic sorbent is prepared from raw peanut hulls. Physical characteristics of the sorbent are detailed, in addition to preliminary laboratory tests under different conditions. The industrial process to produce the sorbent is disclosed. A pressurized underwater injection or aerial dispersion of a water/sorbent mixture is proposed. The possible utilization of the sorbent as a physical dispersant is discussed based on laboratory tests results that reproduce the evolution of the oil/sorbent mass into sea water throughout time. The possible application of the sorbent as complementary tool of the in-situ burning cleanup methodology is also proposed. Finally, the sorbent behavior in front of heavy oils is assessed as a precursor in the formation of tarballs that allow the handling and collection of the pollutant as semi-solid mass offshore. 2 * Student Presenter Pipe Cutting and Plugging Device H. Solis Envigeo, Montreal, QC, Canada This paper refers to a device for cutting and plug subsea fluid conductors under high pressure. The device can be used as an emergency plugging tool to stop leaking fluid into the environment from a damaged oil well. The device works by fixing its housing parallel to the pipe axis with hydraulic clamps. A cutting saw on the end of a rotating arm is activated to cut transversely to the pipe axis. A conical plug concentric with the cutting saw is fixed to the hollow shaft of the hydraulic motor that rotates the saw. After cutting, the plug can be centered by the arm with respect to the axis of the pipe and the plug moved along that axis and inserted into the cut pipe. The fluid drains through the hollow shaft of the hydraulic motor and a swivel to a hose connected to a tank vessel at the surface. Toxic effects of oil and dispersant on growth of the toxic dinoflagellate Karenia brevis M. Moison, D. L. Erdner, E. J. Buskey UTMSI, Port Aransas, TX Karenia brevis is a toxic dinoflagellate species commonly associated with harmful algal blooms in the Gulf of Mexico. The potential effects of oil spills on toxic dinoflagellate blooms are not well understood. We examined the effects of dispersed crude oil on the growth of K. brevis. The Texas Gulf coast experienced a bloom of this toxic dinoflagellate in late summer of 2015. Samples of this natural plankton community were exposed to low and high concentrations of dispersed crude oil and incubated in the laboratory for 7 days. Two culture methods using closed Erlenmeyer’s flasks or open beakers were tested in the same condition and for all treatments. The toxic effects of the dispersant/ crude oil mixture on the growth of K. brevis were measured by direct cell counts. In addition, potential morphological changes were observed using a continuous imaging flow cytometer (FlowCAM). Growth of K. brevis was markedly inhibited by the dispersant/ oil mixture at the highest concentration tested (50ppm). The low concentration treatment (10 ppm) using open beakers showed results similar to the control experiment - the use of open beakers reduces the exposure to the volatile fraction of the crude oil. In contrast, high growth inhibition effects were observed for 10 ppm exposure using closed Erlenmeyer’s flasks. However, morphological changes were observed in the 10 ppm treatment in open beakers; the size of the organism increased and the general shape of the cells were more convoluted, diverging from the typical, more circular shape. Overall, the results demonstrated significant differences depending on the open or closed culture method, and that higher concentrations of dispersed crude oil inhibits the growth of K. brevis, while lower concentrations only affect cell growth in closed containers that retain volatile compounds. Sublethal Effects of Crude Oil Dispersants on a Tidal Creek Crustacean C. P. Nguyen* University of Maryland, College Park, College Park, MD Oil dispersants are often used to mitigate the environmental damage of oil spills with over 2 million gallons used alone during the Deepwater Horizon oil spill in 2010. The growth of oil dispersant use has raised concerns with their long-term effects on the environment and crucial marine organisms and has led to increased research on these issues. In this study, the concentrations of two biomarkers in larvae of the grass shrimp Palaemonetes pugio were measured as a function of oil dispersant concentration 3 * Student Presenter and exposure time. P. pugio play an important role in transporting energy via the food chain in salt marshes and is commonly consumed by economically important fish. The larvae were exposed to two different oil dispersants, Corexit® EC9500A and Finasol® OSR52, in research experiments for 24 hours and 96 hours at various concentrations below lethal levels. The biomarkers of interest were the hormone 20-hydroxyecdysone (20-HE) and the enzyme acetylcholinesterase (AChE), which were measured using the enzyme-linked immunosorbent (ELISA) assay and the acetylcholinesterase assay respectively. These assays gave absorbance values that were then used to calculate concentration and enzyme activity. The hormone 20-HE regulates molting in arthropods. AChE facilitates neurotransmission by cleaving the neurotransmitter acetylcholine but also functions in a developmental role by regulating cell growth and differentiation. Data collected from the 20-HE assay indicated a generally declining trend of 20-HE concentration in P. pugio larvae after 96 hours exposure to oil dispersants, suggesting negative effect on growth. The AChE assay did not indicate any significant effect with exposure to dispersants. Capture and Densification of Floating Crude Oil by Granular Materials D. Boglaienko*, B. Tansel Florida International University, Miami, FL Densification and submergence of floating crude oil in the water column reduces the impact radius of a spill and its mobility, preventing direct contamination of beaches, coastal flora and fauna. Surface application of dry granular materials (quartz sand, limestone) on top of the floating oil layer increases the density of the floating oil phase and leads to formation of relatively large and stable aggregates with a significant amount of oil. The aggregates separate from the floating hydrophobic phase and settle by gravity. The granular densification process has been studied by our research group during the last three years and the summary of the main findings will be presented. The objectives of the series of experiments conducted were to determine the oil removal efficiencies and optimal particle to oil ratio, effect of salinity and the application rates, as well as to study the effects of particle size and particle porosity. In addition to crude oil (South Louisiana crude, MC 252) we analyzed aggregation of quartz sand with other hydrophobic liquids (alkanes and aromatics) to investigate wetting characteristics and relation of aggregation rates to physical properties of liquids, and to investigate dissolution rate constants of the submerged hydrophobic liquids. Classification of the main types of particle-oil aggregates was developed based