Oncogene (2002) 21, 5188 – 5192 ª 2002 Nature Publishing Group All rights reserved 0950 – 9232/02 $25.00 www.nature.com/onc

Deficiency in BRCA2 leads to increase in non-conservative

Florence Larminat*,1, Maryse Germanier1, Efterpi Papouli1 and Martine Defais1

1Institut de Pharmacologie et de Biologie Structurale, UMR 5089, C.N.R.S., 205, Route de Narbonne, 31077 Toulouse Cedex 4, France

The BRCA2 tumor suppressor has been implicated in the al., 1999) and increased sensitivity to genotoxic agents maintenance of genomic integrity through a function in such as X-rays and cross-linking agents (Patel et al., cellular responses to DNA damage. The BRCA2 1998; Yu et al., 2000). Little is known, however, about its directly associates with Rad51, that is essential for precise function. The BRCA2 protein directly associates repair of double-strand breaks (DSBs) by homologous with Rad51 (Sharan et al., 1997), a mammalian recombination (HR). In this report, we study the homologue of the bacterial protein RecA essential for BRCA2-defective Chinese hamster cell mutant V-C8 repair of double-strand breaks (DSBs) by homologous for its ability to perform -directed repair recombination (HR) (Sonoda et al., 1998). Mammalian (HDR) between repeated sequences. V-C8 cells were cells can repair DNA DSBs by both non-homologous recently shown to be defective in Rad51 foci formation in end-joining (NHEJ) and homology-directed repair response to DNA damage. Strikingly, we find that these (HDR) (for review, Baumann and West, 1998). HDR BRCA2 mutant cells exhibit a strong stimulation of can occur through two main mechanisms: conver- HDR activity compared to the V79 parental cells, which sion, that is Rad51-dependent and can take place with or harbor a wild-type BRCA2. Furthermore, molecular without a crossing-over and single – strand annealing characterization of the HDR products shows that loss (SSA), that is Rad51-independent (Lambert and Lopez, of BRCA2 in V-C8 cells leads to significant reduction in 2000). Gene conversion is the preferred repair pathway Rad51-dependent gene conversion but strong enhance- for DSBs by HDR and maintains chromosomal stability ment of Rad51-independent single-strand annealing (Johnson and Jasin, 2000). In response to treatment with (SSA) events frequency. These data imply that, when DNA damaging agents, Rad51 localizes into nuclear foci HDR by conservative gene conversion is impaired, DSBs at sites of DNA damages (Haaf et al., 1995; Tashiro et usually repaired by this pathway are instead resolved by al., 2000). Consistent with a role in DNA repair, other non-conservative HDR subpathways. Therefore, BRCA2-deficient cells exhibit impaired g-rays induced high chromosomal instability in BRCA2-deficient cells Rad51 foci formation (Yuan et al., 1999; Yu et al., 2000) presumably results from enhancement of error-prone and decreased repair of DSBs by gene conversion repair mechanisms, such as SSA. (Moynahan et al., 2001). In addition, BRCA2 appears Oncogene (2002) 21, 5188 – 5192. doi:10.1038/sj.onc. to be necessary for the assembly of Rad51 complexes 1205659 upon DNA damages, by regulating both the intracellular localization and DNA binding activity of Rad51 (Davies Keywords: BRCA2; homologous recombination; DNA et al., 2001). repair; DNA interstrand crosslink; Rad51; single- The radiosensitive Chinese hamster cell mutant V-C8 strand annealing has been recently shown to be defective in the BRCA2 gene (Kraakman-van der Zwet et al, 2002). This cell line shows extreme sensitivity towards DNA cross- linking agents such as mitomycin C (MMC), increased Germ-line mutations in the human BRCA2 breast sensitivity to X-rays (Overkamp et al., 1993) and cancer suppressor gene confer susceptibility to breast display high chromosomal instability (Overkamp et al., and ovarian cancers (Tavtigian et al., 1996; Wooster et 1993; Kraakman-van der Zwet et al, 2002). V-C8 cells al., 1994). The large, nuclear phosphoprotein encoded by have an impaired capacity of repair of DNA DSBs BRCA2 has been implicated in cellular responses to following g-irradiation (Verhaegh et al., 1995) and are DNA damage and in maintaining genomic integrity defective in Rad51-foci formation after DNA damage (Connor et al., 1997; Patel et al., 1998). Several studies (Kraakman-van der Zwet et al, 2002), suggesting that have demonstrated that cells deficient for BRCA2 show they are defective in Rad51-dependent recombination extensive spontaneous chromosomal instability (Tutt et pathways. This prompted us to test the effect of BRCA2 inactivation in V-C8 cells on the frequency of recombinational events. Here we report that BRCA2 *Correspondence: F Larminat; E-mail: fl[email protected] deficiency leads to a general increase of HDR, most Received 18 February 2002; revised 17 April 2002; accepted 10 likely due to enhanced frequency of SSA events, while May 2002 gene conversion events are reduced. Our findings Deficiency in BRCA2 leads to increase in non-conservative HR F Larminat et al 5189 provide evidence for a critical role of BRCA2 in maintaining genome stability. The V-C8 cell line with nonfunctional BRCA2 status is derived from Chinese hamster V79 fibroblasts (Zdzienicka et al., 1990; Overkamp et al., 1993; Kraakman-van der Zwet et al, 2002). To investigate whether the frequency and proportions of HR events are modulated in V-C8 cells, we examined the ability of these cells to perform recombination between two intrachromosomal sequences. Wild-type V79 and mutant V-C8 cells were transfected with the retroviral pNeoA vector (Meyn, 1993), that contains a hygro- mycin resistance gene (hph) for selection of stable transfectants surrounded by two tandemly arranged inactive encoding neomycin resistance (neo)as recombination substrates (Figure 1). Gene conversion, reciprocal exchange and SSA between direct repeats of Figure 2 Rates of spontaneous and MMC-induced HR in V79 mutant neo genes lead to reconstitution of a wild-type and V-C8 cell lines transfected with pNeoA vector. To determine gene and expression of G418 resistance (G418R). spontaneous HR frequency, 104 to 107 hphR cells were seeded per plate and grown in the presence of 400 mg/ml G418, without Recombination rates are calculated from the total 5 R hygromycin. To determine MMC-induced HR frequency, 10 to number of G418 clones and the total number of cells 107 exponentially growing cells per plate were first treated with screened by means of fluctuation analysis tables equitoxic MMC concentrations for 1 h at 378C before growing provided by Capizzi and Jameson (1973). in selection medium containing 400 mg/ml G418, 24 h after drug Following stable integration, spontaneous HR treatment. MMC concentrations used to give 5 to 10% cell survi- between the two neo genes was measured in five val were 5 mg/ml for V79 clones and 0.05 mg/ml for V-C8 clones. A minimum of six plates per seeding density and two different independent transfectants derived from each of V79 seeding densities were used per data point. In parallel, cells were and V-C8 cell lines. All clones were shown to be grown without G418 to determine cloning efficiency and MMC sensitive to G418 at the time of cloning. As shown in cytotoxicity and to estimate the total number of viable cells screened. Recombination rates were derived from the total num- Figure 2, spontaneous HR frequency was low in V79 R 76 ber of G418 colonies obtained and the total number of viable transfectants, averaging 4.75610 event/cell genera- cells screened by means of fluctuation analysis and correspond tion. In contrast, all V-C8 transfectants yielded a to the mean values+s.e. from five independent experiments. Each spontaneous rate of HR whose average was 11161076 experiment was performed with five independent clones derived event/cell generation, 24 times more frequent than in from each cell line. The total number of independent cultures the V79 parental cells (P=0.006, Student t-test) for each cell line was 25 (Figure 2). Our results indicate that BRCA2 mutant cells exhibit a strong stimulation of spontaneous HR activity. These data were not corrected for pNeoA To further characterize HDR events in parental and copy number, that does not significantly modulate BRCA2 cell lines, we examined the molecular products recombination rates between clones (Vispe´ et al., 1998). of the spontaneous reaction leading to the reconstitu- tion of a wild-type neo gene. Representative neoR clones from each hphR transfectant were isolated and analysed by Southern blotting to evaluate the proces- sing of transfected sequences (Figure 3). Gene conversion without crossing-over involves a non- reciprocal transfer of sequence from one neo copy to another and preserves the neo duplication. By contrast, gene conversion with crossing-over and SSA involve loss of the duplication and deletion of sequence between the points of exchange, leading to hygromycin sensitivity. An example of clones demonstrating each type of processing is shown in Figure 3. The molecular Figure 1 Map of the pNeoA recombination vector. The pNeoA analysis of V79 clone 6 illustrates a gene conversion vector contains the hygromycin (hph) resistance gene used to se- event without crossing-over: both neo copies were lect for stable transfectants flanked by two inactive copies of neo- visualized following KpnI restriction in the G418- mycin (neo) resistance gene. The mutations in the neo genes were obtained by insertion of a BamHI linker at a different site in each sensitive (S) clone and in the subsequent G418-resistant gene. The first insertion mutation was introduced into the neo (R) clone; however, one of the neo copy has become gene at the unique NaeI site (3’ neo mutant) and the second at BamHI resistant in this G418-resistant clone (R). The the unique EagI site (5’ neo mutant). Before transfection, pNeoA analysis of V-C8 clone A8 shows a SSA or a crossover was linearized at its unique SspI site to facilitate integration. Re- combination between the two defective genes can lead to a func- event: after KpnI digestion, only one neo copy was tional neo gene and resistance to G418. Restriction sites are K, observed in the G418-resistant (R) clone, which was KpnI and B, BamHI BamHI resistant. Additional BamHI bands are seen in

Oncogene Deficiency in BRCA2 leads to increase in non-conservative HR F Larminat et al 5190 this clone, which are probably due to multiple neo dent crossing-over events. Thus, our data suggest that integrations (Figure 3). The molecular characterization loss of BRCA2 in V-C8 cells leads to significant of independent G418R recombinants is indicated in reduction in gene conversion but strong enhancement Table 1. All V79 clones (15 out of 15) achieved G418 of SSA events frequency, resulting in an overall resistance via gene conversion. Eight out of 14 V-C8 increase in HDR. However, we found that gene clones showed evidence for gene conversion, while 6 conversion still can occur despite the absence of out of 14 have undergone a crossover event or SSA functional BRCA2. By contrast, inhibition of func- and lost hygromycin resistance (Table 1). Our results tional Rad51 by expression of a dominant negative clearly show that the ratio of conservative gene Rad51 protein strongly decreases gene conversion conversion to exchange events and/or SSA is signifi- frequency (Lambert and Lopez, 2000). cantly diminished in V-C8 cells, as compared to V79 Since V-C8 cells are hypersensitive to cross-linking cells. Since V-C8 cells have been shown to be defective agents, we then tested whether MMC stimulates HDR in Rad51-foci formation following DNA damage between the two tandem repeat neo sequences and (Kraakman-van der Zwet et al., 2002) and HR repair whether BRCA2 deficiency affects MMC-induced by exchange events requires Rad51 (Baumann and HDR. MMC interstrand cross-links are known to West, 1998), a plausible hypothesis is that deletions of induce SCE by the Rad51-dependent pathway (Sonoda hygromycin resistance are rather due to the Rad51- et al., 1999). Twenty-four hours before selection of independent SSA pathway and not to Rad51-depen- G418R clones, hphR transfectants from V79 and V-C8 cell lines were treated with equitoxic MMC concentra- tions, until survival was reduced to 5 – 10% of untreated cells. As expected and shown in Figure 2, MMC treatment resulted in a significant increase in the frequency of G418-resistant recombinants in all V79 clones studied. HR frequency was increased by factor of six from 4.7561076 to 28.861076 event/cell generation (P50.0001, Student’s t-test) (Figure 2). By contrast, only a weak induction as compared to the untreated cells value was observed after MMC treatment in all V- C8 clones analysed (Figure 2). Yet, the MMC-induced HR frequency remains consistently higher in the BRCA2-deficient cells than in the wild-type cells. We have obtained evidence demonstrating that loss of wild-type BRCA2 increases spontaneous HDR in V- C8 cells, most likely due to stimulation of SSA events, while gene conversion frequency is significantly decreased when compared with the wild-type V79 cell line. These data are consistent with failure of normal MMC-induced Rad51 foci formation in V-C8 cells Figure 3 Processing of pNeoA to achieve G418-resistance. (Kraakman-van der Zwet et al., 2002), suggesting that Genomic DNA from representative G418-sensitive (S) and Rad51 functions are impaired in those cells. Rad51 has G418-resistant (R) clones were digested with KpnIorBamHI been shown to have a role in gene conversion, but not and hybridized with a 32P-labeled probe consisting of the 1.2-kb KpnI-fragment of the pNeoA vector. Gene conversion without in SSA pathway (Lambert and Lopez, 2000) and to crossing-over products yield 3.2- and 1.23 kb KpnI-fragments interact with BRCA2, which affects its subcellular and 1.2- and 0.95- or 0.8-kb BamHI-fragments, as shown with localization (Davies et al., 2001). Loss of wild-type the V79-6 clone. Crossovers and/or SSA events result in the loss BRCA2 in V-C8 cells would then impair Rad51 of the duplication and release a single KpnI-fragment which is nucleoprotein filament formation necessary for BamHI resistant, as shown with the VC8-A8 clone Rad51-dependent HR events, such as gene conversion. The results presented here are consistent with this notion. Accordingly, BRCA2-deficient human Table 1 Spontaneous frequency of gene conversion in G418R CAPAN-1 or mouse ES cells show a reduction in the recombinants frequency of HR repair due to gene conversion Cell Clones Gene Exchange/ % of gene (Moynahan et al., 2001). On the other hand, line tested conversion SSA conversion inactivation of wild-type BRCA2 in V-C8 cells greatly enhances the frequency of SSA events, which certainly V79 15 15 0 100 VC8 14 8 6 57 contributes to the increase in overall HDR that we observe in those cells. Such phenotype has been Each spontaneous recombinant was isolated from an independent recently reported by Tutt et al. (2001), who demon- subpopulation derived from fluctuation tests. Gene conversion strated that the conditional loss of BRCA2 increases preserves the neo duplication and is detected as 2 KpnI fragments; crossing-overs and SSA events result in a single wild-type copy of the HDR repair by SSA in mouse ES cells. This study and neo gene and are detected as a single KpnI-fragment, which is BamHI our data imply that, when HDR by gene conversion is resistant disrupted, DSBs usually repaired by this pathway are

Oncogene Deficiency in BRCA2 leads to increase in non-conservative HR F Larminat et al 5191 instead resolved by other HDR subpathways such as processing of DNA interstrand cross-links. Although SSA. Increase of HDR by SSA may cause deletions interstrand cross-link repair mechanism is not yet well and chromosomal translocations (Richardson and characterized in mammalian cells, this pathway is Jasin, 2000). Our findings are consistent with the thought to involve a sequential action of excision extreme chromosomal instability displayed by V-C8 repair and HR (for review, Dronkert and Kanaar, cells (Overkamp et al., 1993). Spontaneous chromoso- 2001). DSBs are presumably produced as repair mal exchanges in V-C8 are mainly of chromatid type intermediates and are repaired by HR involving the and the majority of spontaneous mutations in V-C8 Rad51-related XRCC2 and XRCC3 (De Silva cells at the hprt locus are deletions (Kraakman-van der et al., 2000; Liu et al., 1998). Our study suggests that, Zwet et al., 2002), while in wild-type V79 cells mainly in absence of wild-type BRCA2, interstrand cross-links base substitutions are found (Zhang et al., 1992). In could be repaired by non-conservative SSA rather than summary, the inactivation of BRCA2 leads to genetic conservative gene conversion, leading to high chromo- instability, which presumably results from enhance- somal instability. Thus, the ability of antitumor drugs ment of error-prone repair mechanisms, such as SSA. to induce interstrand cross-links may be of particular This suggests that BRCA2 is more likely to be a interest in the treatment of BRCA2-deficient cancer regulator of HR levels, rather than an effector, such as patients. Rad51. V-C8 cells are extremely sensitive to DNA cross- linking agents (Overkamp et al., 1993) and display increased apoptotic response to DNA damages induced Acknowledgments by MMC (Papouli et al., 2000). Hypersensitivity to We thank Dr MZ Zdzienicka for generously providing us DNA cross-linking agents, such as MMC has also been with the V-C8 cell line and Dr S Meyn for providing the observed in BRCA2-deficient mouse cells (Yu et al., pNeoA vector. This work was supported by the Associa- 2000). This suggests that BRCA2 is involved in tion pour la Recherche sur le Cancer grant 9238.

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