MAP3K12 (Human) Recombinant Protein

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MAP3K12 (Human) Recombinant Gene Alias: DLK, MUK, ZPK, ZPKP1 Protein Gene Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This Catalog Number: P5535 kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The Regulation Status: For research use only (RUO) phosphorylation state of this kinase in synaptic terminals Product Description: Human MAP3K12 (NP_006292.3, was shown to be regulated by membrane depolarization 1 a.a. - 520 a.a.) partial recombinant protein with GST via calcineurin. This kinase forms heterodimers with tag expressed in baculovirus infected Sf21 cells. leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and Host: Insect MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. [provided Theoretical MW (kDa): 86 by RefSeq] Applications: Func, SDS-PAGE (See our web site product page for detailed applications information) Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols Form: Liquid Preparation Method: Baculovirus infected insect cell (Sf21) expression system Purification: Glutathione sepharose chromatography Purity: 55 % by SDS-PAGE/CBB staining Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM. Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol) Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 7786 Gene Symbol: MAP3K12 Page 1/1 Powered by TCPDF (www.tcpdf.org).

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MEK-7 Polyclonal Antibody Catalog No : YT2725 Reactivity : Human,Mouse,Rat Applications : WB,ELISA Gene Name : MAP2K7 Protein Name : Dual specificity mitogen-activated protein kinase kinase 7 Human Gene Id : 5609 Human Swiss Prot O14733 No : Mouse Gene Id : 26400 Mouse Swiss Prot Q8CE90 No : Rat Gene Id : 363855 Rat Swiss Prot No : Q4KSH7 Immunogen : The antiserum was produced against synthesized peptide derived from human MAP2K7. AA range:241-290 Specificity : MEK-7 Polyclonal Antibody detects endogenous levels of MEK-7 protein. Formulation : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source : Rabbit Dilution : Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. Purification : The antibody was affinity-purified from rabbit antiserum by affinity- chromatography using epitope-specific immunogen. Concentration : 1 mg/ml 1 / 3 Storage Stability : -20°C/1 year Molecularweight : 47485 Observed Band : 47 Cell Pathway : MAPK_ERK_Growth,MAPK_G_Protein,ErbB_HER,Toll_Like,T_Cell_Receptor, Fc epsilon RI,Neurotrophin,GnRH, Background : mitogen-activated protein kinase kinase 7(MAP2K7) Homo sapiens The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell
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Microtopographical Cues Promote Peripheral Nerve Regeneration Via Transient Mtorc2 Activation ⇑ Suzanne E
Acta Biomaterialia 60 (2017) 220–231 Contents lists available at ScienceDirect Acta Biomaterialia journal homepage: www.elsevier.com/locate/actabiomat Full length article Microtopographical cues promote peripheral nerve regeneration via transient mTORC2 activation ⇑ Suzanne E. Thomson a,b, , Chloe Charalambous a, Carol-Anne Smith a, Penelope M. Tsimbouri a, Theophile Déjardin a,c, Paul J. Kingham d, Andrew M. Hart a,b,d, Mathis O. Riehle a a The Centre for Cell Engineering, College of Medical, Veterinary and Life Sciences, University of Glasgow, University Avenue, Hillhead, Glasgow G12 8QQ, UK b Canniesburn Plastic Surgery Unit, Glasgow Royal Inﬁrmary, 84 Castle Street, Glasgow G4 0SF, UK c Institut Jacques Monod CNRS UMR 7592, Université Paris Diderot, Paris, France d Dept. of Integrative Medical Biology, Umeå University, SE-901 87 Umeå, Sweden article info abstract Article history: Despite microsurgical repair, recovery of function following peripheral nerve injury is slow and often Received 31 March 2017 incomplete. Outcomes could be improved by an increased understanding of the molecular biology of Received in revised form 4 July 2017 regeneration and by translation of experimental bioengineering strategies. Topographical cues have been Accepted 20 July 2017 shown to be powerful regulators of the rate and directionality of neurite regeneration, and in this study Available online 25 July 2017 we investigated the downstream molecular effects of linear micropatterned structures in an organotypic explant model. Linear topographical cues enhanced neurite outgrowth and our results demonstrated that Keywords: the mTOR pathway is important in regulating these responses. Microtopography mTOR gene expression peaked between 48 and 72 h, coincident with the onset of rapid neurite out- Peripheral nerve Tissue engineering growth and glial migration, and correlated with neurite length at 48 h.
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PRODUCTS and SERVICES Target List
PRODUCTS AND SERVICES Target list Kinase Products P.1-11 Kinase Products Biochemical Assays P.12 "QuickScout Screening Assist™ Kits" Kinase Protein Assay Kits P.13 "QuickScout Custom Profiling & Panel Profiling Series" Targets P.14 "QuickScout Custom Profiling Series" Preincubation Targets Cell-Based Assays P.15 NanoBRET™ TE Intracellular Kinase Cell-Based Assay Service Targets P.16 Tyrosine Kinase Ba/F3 Cell-Based Assay Service Targets P.17 Kinase HEK293 Cell-Based Assay Service ～ClariCELL™ ～ Targets P.18 Detection of Protein-Protein Interactions ～ProbeX™～ Stable Cell Lines Crystallization Services P.19 FastLane™ Structures ～Premium～ P.20-21 FastLane™ Structures ～Standard～ Kinase Products For details of products, please see "PRODUCTS AND SERVICES" on page 1～3. Tyrosine Kinases Note: Please contact us for availability or further information. Information may be changed without notice. Expression Protein Kinase Tag Carna Product Name Catalog No. Construct Sequence Accession Number Tag Location System HIS ABL(ABL1) 08-001 Full-length 2-1130 NP_005148.2 N-terminal His Insect (sf21) ABL(ABL1) BTN BTN-ABL(ABL1) 08-401-20N Full-length 2-1130 NP_005148.2 N-terminal DYKDDDDK Insect (sf21) ABL(ABL1) [E255K] HIS ABL(ABL1)[E255K] 08-094 Full-length 2-1130 NP_005148.2 N-terminal His Insect (sf21) HIS ABL(ABL1)[T315I] 08-093 Full-length 2-1130 NP_005148.2 N-terminal His Insect (sf21) ABL(ABL1) [T315I] BTN BTN-ABL(ABL1)[T315I] 08-493-20N Full-length 2-1130 NP_005148.2 N-terminal DYKDDDDK Insect (sf21) ACK(TNK2) GST ACK(TNK2) 08-196 Catalytic domain
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Xo PANEL DNA GENE LIST
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Downloaded from the GDSC Database 76
bioRxiv preprint doi: https://doi.org/10.1101/2020.06.18.158907; this version posted November 18, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A regularized functional regression model enabling transcriptome-wide dosage-dependent association study of cancer drug response Biomarker detection for revealing anticancer drug dynamics Evanthia Koukouli1*, Dennis Wang 2,3, Frank Dondelinger4Y, Juhyun Park1Y 1 Department of Mathematics and Statistics, Fylde College, Lancaster University, Bailrigg, Lancaster, UK 2 Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK 3 Department of Computer Science, University of Sheffield, Sheffield, UK 4 Centre for Health Informatics and Statistics, Lancaster Medical School, Lancaster University, Bailrigg, Lancaster, UK YThese authors contributed equally to this work. *[email protected] (EK) Abstract Cancer treatments can be highly toxic and frequently only a subset of the patient population will benefit from a given treatment. Tumour genetic makeup plays an important role in cancer drug sensitivity. We suspect that gene expression markers could be used as a decision aid for treatment selection or dosage tuning. Using in vitro cancer cell line dose-response and gene expression data from the Genomics of Drug Sensitivity in Cancer (GDSC) project, we build a dose-varying regression model. Unlike existing approaches, this allows us to estimate dosage-dependent associations with gene expression. We include the transcriptomic profiles as dose-invariant covariates into the regression model and assume that their effect varies smoothly over the dosage levels.
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Computer Vision Profiling of Neurite Outgrowth Dynamics Reveals Spatiotemporal Modularity of Rho Gtpase Signaling
Published January 4, 2016 JCB: ArticleTools Computer vision profiling of neurite outgrowth dynamics reveals spatiotemporal modularity of Rho GTPase signaling Ludovico Fusco,1* Riwal Lefort,2* Kevin Smith,3* Fethallah Benmansour,3 German Gonzalez,3 Caterina Barillari,4 Bernd Rinn,4 Francois Fleuret,2 Pascal Fua,3 and Olivier Pertz1 1Department of Biomedicine, University of Basel, 4058 Basel, Switzerland 2Institut Dalla Molle d'Intelligence Artificielle Perceptive (IDI AP Research Institute), 1920 Martigny, Switzerland 3Computer Vision Laboratory, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland 4Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule, 4058 Basel, Switzerland Rho guanosine triphosphatases (GTPases) control the cytoskeletal dynamics that power neurite outgrowth. This process consists of dynamic neurite initiation, elongation, retraction, and branching cycles that are likely to be regulated by Downloaded from specific spatiotemporal signaling networks, which cannot be resolved with static, steady-state assays. We present Neu- riteTracker, a computer-vision approach to automatically segment and track neuronal morphodynamics in time-lapse datasets. Feature extraction then quantifies dynamic neurite outgrowth phenotypes. We identify a set of stereotypic neurite outgrowth morphodynamic behaviors in a cultured neuronal cell system. Systematic RNA interference perturba- tion of a Rho GTPase interactome consisting of 219 proteins reveals a limited set of morphodynamic phenotypes. As proof of concept, we show that loss of function of two distinct RhoA-specific GTPase-activating proteins (GAPs) leads to jcb.rupress.org opposite neurite outgrowth phenotypes. Imaging of RhoA activation dynamics indicates that both GAPs regulate differ- ent spatiotemporal Rho GTPase pools, with distinct functions. Our results provide a starting point to dissect spatiotempo- ral Rho GTPase signaling networks that regulate neurite outgrowth.
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ATAP01752-Recombinant Human MAP2K7 Protein
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Dema and Faust Et Al., Suppl. Material 2020.02.03
Supplementary Materials Cyclin-dependent kinase 18 controls trafficking of aquaporin-2 and its abundance through ubiquitin ligase STUB1, which functions as an AKAP Dema Alessandro1,2¶, Dörte Faust1¶, Katina Lazarow3, Marc Wippich3, Martin Neuenschwander3, Kerstin Zühlke1, Andrea Geelhaar1, Tamara Pallien1, Eileen Hallscheidt1, Jenny Eichhorst3, Burkhard Wiesner3, Hana Černecká1, Oliver Popp1, Philipp Mertins1, Gunnar Dittmar1, Jens Peter von Kries3, Enno Klussmann1,4* ¶These authors contributed equally to this work 1Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert- Rössle-Strasse 10, 13125 Berlin, Germany 2current address: University of California, San Francisco, 513 Parnassus Avenue, CA 94122 USA 3Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Strasse 10, 13125 Berlin, Germany 4DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Oudenarder Strasse 16, 13347 Berlin, Germany *Corresponding author Enno Klussmann Max Delbrück Center for Molecular Medicine Berlin in the Helmholtz Association (MDC) Robert-Rössle-Str. 10, 13125 Berlin Germany Tel. +49-30-9406 2596 FAX +49-30-9406 2593 E-mail: [email protected] 1 Content 1. CELL-BASED SCREENING BY AUTOMATED IMMUNOFLUORESCENCE MICROSCOPY 3 1.1 Screening plates 3 1.2 Image analysis using CellProfiler 17 1.4 Identification of siRNA affecting cell viability 18 1.7 Hits 18 2. SUPPLEMENTARY TABLE S4, FIGURES S2-S4 20 2 1. Cell-based screening by automated immunofluorescence microscopy 1.1 Screening plates Table S1. Genes targeted with the Mouse Protein Kinases siRNA sub-library. Genes are sorted by plate and well. Accessions refer to National Center for Biotechnology Information (NCBI, BLA) entries. The siRNAs were arranged on three 384-well microtitre platres.
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Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM 014911.2 KIAA1048, Dkfzp686k16132 AP2 Associated Kinase 1
Gene Symbol Accession Alias/Prev Symbol Official Full Name AAK1 NM_014911.2 KIAA1048, DKFZp686K16132 AP2 associated kinase 1 (AAK1) AATK NM_001080395.2 AATYK, AATYK1, KIAA0641, LMR1, LMTK1, p35BP apoptosis-associated tyrosine kinase (AATK) ABL1 NM_007313.2 ABL, JTK7, c-ABL, p150 v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1) ABL2 NM_007314.3 ABLL, ARG v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2) ACVR1 NM_001105.2 ACVRLK2, SKR1, ALK2, ACVR1A activin A receptor ACVR1B NM_004302.3 ACVRLK4, ALK4, SKR2, ActRIB activin A receptor, type IB (ACVR1B) ACVR1C NM_145259.2 ACVRLK7, ALK7 activin A receptor, type IC (ACVR1C) ACVR2A NM_001616.3 ACVR2, ACTRII activin A receptor ACVR2B NM_001106.2 ActR-IIB activin A receptor ACVRL1 NM_000020.1 ACVRLK1, ORW2, HHT2, ALK1, HHT activin A receptor type II-like 1 (ACVRL1) ADCK1 NM_020421.2 FLJ39600 aarF domain containing kinase 1 (ADCK1) ADCK2 NM_052853.3 MGC20727 aarF domain containing kinase 2 (ADCK2) ADCK3 NM_020247.3 CABC1, COQ8, SCAR9 chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1) ADCK4 NM_024876.3 aarF domain containing kinase 4 (ADCK4) ADCK5 NM_174922.3 FLJ35454 aarF domain containing kinase 5 (ADCK5) ADRBK1 NM_001619.2 GRK2, BARK1 adrenergic, beta, receptor kinase 1 (ADRBK1) ADRBK2 NM_005160.2 GRK3, BARK2 adrenergic, beta, receptor kinase 2 (ADRBK2) AKT1 NM_001014431.1 RAC, PKB, PRKBA, AKT v-akt murine thymoma viral oncogene homolog 1 (AKT1) AKT2 NM_001626.2 v-akt murine thymoma viral oncogene homolog 2 (AKT2) AKT3 NM_181690.1
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Determining Mechanisms of Response to Polo-Like Kinase 1 Inhibition in Non-Small Cell Lung Cancer
The Texas Medical Center Library DigitalCommons@TMC The University of Texas MD Anderson Cancer Center UTHealth Graduate School of The University of Texas MD Anderson Cancer Biomedical Sciences Dissertations and Theses Center UTHealth Graduate School of (Open Access) Biomedical Sciences 8-2016 Determining Mechanisms of Response to Polo-like Kinase 1 Inhibition in Non-Small Cell Lung Cancer Ruchitha Goonatilake Follow this and additional works at: https://digitalcommons.library.tmc.edu/utgsbs_dissertations Part of the Medicine and Health Sciences Commons Recommended Citation Goonatilake, Ruchitha, "Determining Mechanisms of Response to Polo-like Kinase 1 Inhibition in Non- Small Cell Lung Cancer" (2016). The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access). 700. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/700 This Thesis (MS) is brought to you for free and open access by the The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences at DigitalCommons@TMC. It has been accepted for inclusion in The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access) by an authorized administrator of DigitalCommons@TMC. For more information, please contact [email protected]. DETERMINING MECHANISMS OF RESPONSE TO POLO-LIKE KINASE 1 INHIBITION IN NON-SMALL CELL LUNG CANCER by Ruchitha Goonatilake, BS APPROVED: ______________________________
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Anti-MAP3K12 Antibody
D121305 Anti-MAP3K12 antibody Cat. No. D121305 Package 25 μl/100 μl/200 μl Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Product overview Description A n ti-MAP3K12 rabbit polyclonal antibody Applications ELISA, IHC Immunogen Fusion protein of human MAP3K12 Reactivity Human, Mouse, Rat Content 0 .2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification Target information Symbol MAP3K12 Full name mitogen-activated protein kinase kinase kinase 12 Synonyms DLK; MUK; ZPK; ZPKP1; MEKK12 Swissprot Q12852 Target Background This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins haSve beeangonn described. Biotech Applications Immunohistochemistry Predicted cell location: Cytoplasm Predicted cell location: Cytoplasm Positive control: Human colon cancer Positive control: Human thyroid cancer Recommended dilution: 25-100 Recommended dilution: 25-100 The image on the left is immunohistochemistry of paraffin-embedded The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D121305(MAP3K12 Antibody) at Human thyroid cancer tissue using D121305(MAP3K12 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original dilution 1/30, on the right is treated with fusion protein.
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