Data Sheet (Cat.No.T4464)

Irosustat Chemical Properties

CAS No.: 288628-05-7 Formula： C14H15NO5S Molecular Weight： 309.34 Appearance： N/A Storage： 0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Biological Description

Description Irosustat is a potent sulfatase inhibitor, with an IC50 of 8 nM, and exhibits anti- cancer activity. In vitro Irosustat (667 COUMATE) is a potent inhibitor, with an IC50 of 8 nM. Irosustat (667 COUMATE) inhibits steroid sulphatase (STS) activity in MCF-7 cells with an IC50 of 0.2 nM, but has no effect on the morphology or proliferation of MCF-7 cells at 10 μM In vivo Irosustat potently inhibits rat liver, with inhibition of >90% when at a 1 mg/kg concentration. Irosustat (2 mg/kg, p.o. for 5 d) blocks the uterine growth stimulated by oestrone (E1S) in ovariectomized rats. In addition, Irosustat (2, 10 mg/kg, p.o.) plus E1S dose-dependently decreases the growth of NMU-induced mammary tumors in ovariectomized rats. Irosustat (667 COUMATE; 10 mg/kg, p.o.) shows 97.9 ± 0.06% inhibition on steroid sulphatase (STS) activity in rat liver

Solubility Information

Solubility DMSO: 150 mg/mL (< 1 mg/ml refers to the product slightly soluble or insoluble)

Preparing Stock Solutions 1mg 5mg 10mg

1 mM 3.233 mL 16.163 mL 32.327 mL 5 mM 0.647 mL 3.233 mL 6.465 mL 10 mM 0.323 mL 1.616 mL 3.233 mL 50 mM 0.065 mL 0.323 mL 0.647 mL Please select the appropriate solvent to prepare the stock solution, according to the solubility of the product in different solvents. The storage conditions and period of the stock solution: - 80 ℃ for 6 months; - 20 ℃ for 1 month. Please use it as soon as possible.

Reference 1. Purohit A,etal.In vivo inhibition of sulfatase activity and growth of nitrosomethylurea-induced mammary tumors by 667 COUMATE.Cancer Res. 2000 Jul 1;60(13):3394-6. 2. Raobaikady B,etal.Inhibition of MCF-7 cell proliferation and in vivo steroid sulphatase activity by 2-methoxyoestradiol- bis-sulphamate.J Steroid Biochem Mol Biol. 2003 Feb;84(2-3):351-8.

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