DOCSLIB.ORG

Serum Fatty Acid-Binding Protein 4 Is Increased in Patients with Psoriasis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Serum Fatty Acid-Binding Protein 4 Is Increased in Patients with Psoriasis Lipids DOI 10.1007/s11745-016-4211-4 ORIGINAL ARTICLE Serum Fatty Acid-Binding Protein 4 is Increased in Patients with Psoriasis A. Baran1 · M. S´widerska2 · Joanna Bacharewicz-Szczerbicka1 · H. Mys´liwiec1 · I. Flisiak1 Received: 4 August 2016 / Accepted: 2 November 2016 © The Author(s) 2016. This article is published with open access at Springerlink.com Abstract Psoriasis is associated with metabolic syndrome ml] (p 0.12), losing its basal significance. FABP4 may be = and cardiovascular disease. Fatty acid-binding proteins a marker of psoriasis, and FABP3 may be associated with (FABP) have been recognized as predictors of these sys- inflammation or liver disorders in psoriatic patients. FABP temic disorders. The aim of this study was to assess cor- do not appear to be useful for determining disease severity relations between levels of serum heart and adipocyte or the effectiveness of antipsoriatic treatment. fatty acid-binding proteins (FABP3, FABP4) and disease severity, indicators of inflammation or metabolic distur- Keywords Psoriasis · Fatty acid-binding protein 3 · bances, and topical treatment in psoriatic patients. Thirty- Fatty acid-binding protein 4 · Anthralin · Lipids · seven patients with relapse of plaque-type psoriasis and Metabolic syndrome 16 healthy volunteers were recruited. Blood samples were collected before and after 14 days of therapy. Serum FABP Abbreviations concentrations were examined by enzyme-linked immu- aP2 Adipocyte protein 2 nosorbent assay for correlation with Psoriasis Area and BMI Body mass index Severity Index (PASI), body mass index (BMI), inflam- CAD Coronary artery disease matory or metabolic parameters, and treatment used. The CRP C-reactive protein median FABP4 serum levels were significantly increased DM Diabetes mellitus (p 0.038) in psoriatic patients, while FABP3 levels did ELISA Enzyme-linked immunosorbent assay = not differ (p 0.47) compared to the controls. No sig- FA Fatty acids = nificant correlations were noted between the proteins and FABP Fatty acid-binding proteins PASI, C-reactive protein (CRP), BMI, or levels of glu- FABP1 Liver fatty acid-binding protein cose or lipids. FABP3 significantly correlated with white FABP3 Heart fatty acid-binding protein blood count (p 0.03) and aspartate aminotransferase FABP4 Adipocyte fatty acid-binding protein = (p 0.04). After topical treatment, there was no significant FABP5 Epidermal fatty acid-binding protein = change in serum FABP3 [11.5 (4.9–30.3) vs. 12.9 (3.5– HDL-C High-density lipoprotein cholesterol 30.3) ng/ml] (p 0.96), whereas FABP4 was decreased IR Insulin resistance = [27,286 (20,344–32,257) vs. 23,034 (18,320–29,874) pg/ L-FABP Liver fatty acid-binding protein LCFA Long-chain fatty acids * A. Baran LDL-C Low-density lipoprotein cholesterol [email protected] MI Myocardial infarction MS Metabolic syndrome 1 Department of Dermatology and Venereology, Medical NAFLD Non-alcoholic fatty liver disease University of Bialystok, Zurawia 14 St, 15‑540 Bialystok, Poland NB-UVB Narrow-band ultraviolet B PA-FABP Psoriasis-associated fatty acid-binding protein 2 Department of Infectious Diseases and Hepatology, Medical University of Bialystok, Zurawia 14 St, 15‑540 Bialystok, PASI Psoriasis Area and Severity Index Poland PLT Platelets 1 3 Lipids RBC Red blood count named based on the tissues in which they are prominently RBP-4 Retinol-binding protein 4 expressed or those that actively participate in lipid metabo- SR Sedimentation rate lism: liver, intestines, heart, adipose tissue, epidermis, ileus, TAG Triacylglycerol brain, myelin and testis. FABP isoforms are thought to be TNF-α Tumor necrosis factor-α useful indicators of organ damage and to play an important VEGF Vascular endothelial growth factor role in the development of a number of systemic diseases. WBC White blood cell FABP1 (liver FABP), present mainly in hepatocytes and excreted by the kidneys, indicates acute kidney damage and liver disorders, while the ileal isoform may be associated Introduction with ileitis or IR [10]. Epidermal fatty acid-binding protein 5 (FABP5), with its Psoriasis is a chronic inflammatory dermatosis with a mul- gene located at chromosome 8q21.13, is mostly expressed tifactorial pathogenesis, affecting over 2% of the world’s in epidermal cells, but also in other tissues including the population. Recently it has been considered as a systemic brain, kidney, liver, lungs, and testis and in adipose tissue. disorder closely associated with coronary artery disease Various studies have stressed its role in regulating insulin (CAD), hypertension, diabetes mellitus (DM), obesity, sensitivity, lipid homeostasis, MS, skin barrier conditions, metabolic syndrome (MS) and atherosclerosis [1, 2]. Pso- and even nerve regeneration [8, 10, 12, 13]. FABP5 is riatic patients live on average 5 years less than the general also called psoriasis-associated FABP (PA-FABP), which population, which is due to increased risk of myocardial reflects its already proven links with this dermatosis [12, infarction (MI) and thromboembolic events [3]. The risk 13]. Some studies have indicated that FABP5 modulates of developing DM, obesity and MS is more than double, the differentiation of keratinocytes and is highly over- occurring in over 40% of psoriatic patients [4]. Com- expressed in psoriatic plaques or atopic dermatitis [12, 13]. mon links between psoriasis and its comorbidities mainly Heart-type FABP (FABP3), with the encoding gene involve the chronic inflammation that emerges from meta- located on chromosome 1p33–p31, is expressed mainly bolic tissues (metaflammation), genetic basis, similar path- in the heart, skeletal muscle, brain, mammary glands and ways of immune disorders, and bioactive substances syn- brown adipose tissue. FABP3 is superior to troponin as a thesized and secreted by adipose tissue [4–6]. Numerous highly sensitive marker of acute myocardial infarction and published studies, as well as ours, have demonstrated the predictor of heart failure in patients with MS, which is potential role of various adipokines in the development of strongly associated with psoriasis [8, 10]. FABP3 also par- psoriasis and its comorbidities [5, 7]. ticipates in thermoregulation and affects glucose homeosta- Lipids, such as fatty acids (FA) and their derivatives, sis and cardiac sufficiency [9, 10]. A precise role of FABP3 perform various biological functions, including their role in other tissues and the development of certain diseases is in energy homeostasis, the formation of substrates for cell still largely unknown. membranes, and signaling molecules in inflammatory and Adipocyte fatty acid-binding protein (FABP4), also metabolic pathways [8]. Disturbances in lipid homeostasis termed adipocyte protein 2 (aP2), is an adipokine synthe- can lead to numerous common disorders including obesity, sized and released predominantly from adipocytes and, to insulin resistance (IR), DM, cardiovascular disease, and a lesser extent, produced in macrophages and endothelial even skin disorders [9, 10]. Fatty acids, which constitute cells [6, 14]. FABP4, with a molecular mass of 14.6 kDa about 15% of lipids in the stratum corneum, participate in and 132 amino acids, accounts for up to 6% of total cellu- maintaining the permeability of the epidermis, for example, lar proteins. The gene encoding this adipokine is located on by stimulating acidification of the horny layer. Research chromosome 8q21. Elevated levels of FABP4 are closely has shown that deviations in FA composition in keratino- linked with the development of obesity, IR, DM, hyper- cytes may be involved in the pathogenesis of inflammatory tension, CAD and atherosclerosis [10, 14, 15]. Studies in dermatosis such as atopic dermatitis or psoriasis [11]. FA humans have shown that a genetic variant at the FABP4 shifted to long-chain fatty acids (LCFA) are transported by locus reduced the risk for atherosclerosis, diabetes and cor- certain proteins to various tissues, where they are metabo- onary heart disease [16, 17], and reduced FABP4 expres- lized, stored or utilized [9]. Fatty acid-binding proteins sion in adipose tissue was related to lower risk for CAD (FABP), first identified in 1972, are a 14–15 kDa family and DM [16]. Several studies have proved that increased of cytosol proteins involved in active regulation of lipid circulating FABP4 levels are a promising biomarker pre- trafficking and the protection of organisms against harm- dicting heart failure, stroke, and prognosis and mortality in ful accumulation of LCFA [8, 10, 11]. To date, at least patients with end-stage renal disease, CAD or other critical nine isoforms have been identified, and they have been illnesses [6, 14, 15, 20, 21]. 1 3 Lipids Another possible role of FABP4 in psoriasis is evident in Serum Collection the influence on MS, which is strongly associated with pso- riasis. FABP4 is an independent predictive marker for the Blood samples were collected from the study and control development of MS, as was shown in a 5-year prospective groups using Vacutainer tubes, and were left for 30 min to study and in a study of Korean boys [18, 19]. allow clotting before centrifugation for 15 min at 1000g, A further link between FABP4 and psoriasis may be a after which the serum was separated and stored at 80 °C − correlation with tumor necrosis factor-α (TNF-α), which is until use. FABP3 and FABP4 levels were measured using one of the major cytokines involved in the pathogenesis of the Quantikine® enzyme immunoassay kit (R&D Sys- this dermatosis. FABP4-deficient macrophages
Load more

Recommended publications
  • Mice Subjected to Ap2-Cre Mediated Ablation of Microsomal Triglyceride Transfer Protein Are Resistant to High Fat Diet Induced Obesity Ahmed Bakillah1,2* and M
    Bakillah and Hussain Nutrition & Metabolism (2016) 13:1 DOI 10.1186/s12986-016-0061-6 RESEARCH Open Access Mice subjected to aP2-Cre mediated ablation of microsomal triglyceride transfer protein are resistant to high fat diet induced obesity Ahmed Bakillah1,2* and M. Mahmood Hussain1,2,3 Abstract Background: Microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. MTP has been shown on the surface of lipid droplets of adipocytes; however its function in adipose tissue is not well defined. We hypothesized that MTP may play critical role in adipose lipid droplet formation and expansion. Methods: Plasmids mediated overexpression and siRNA mediated knockdown of Mttp gene were performed in 3T3-L1 pre-adipocytes to evaluate the effects of MTP on cell differentiation and triglyceride accumulation. Adipose- specific knockdown of MTP was achieved in mice bybreeding MTP floxed (Mttpfl/fl) mice with aP2-Cre recombinase transgenic mice. Adipose-specific MTP deficient (A-Mttp-/-) mice were fed 60 % high-fat diet (HFD), and the effects of MTP knockdown on body weight, body fat composition, plasma and tissues lipid composition, glucose metabolism, lipogenesis and intestinal absorption was studied. Lipids were measured in total fasting plasma and size fractionated plasma using colorimetric assays. Gene expression was investigated by Real-Time quantitative PCR. All data was assessed using t-test, ANOVA. Results: MTP expression increased during early differentiation in 3T3-L1 cells, and declined later. The increases in MTP expression preceded PPARγ expression. MTP overexpression enhanced lipid droplets formation, and knockdown attenuated cellular lipid accumulation. These studies indicated that MTP positively affects adipogenesis.
    [Show full text]
  • Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals
    animals Review Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals Mohammadreza Mohammadabadi 1 , Farhad Bordbar 1,* , Just Jensen 2 , Min Du 3 and Wei Guo 4 1 Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman 77951, Iran; [email protected] 2 Center for Quantitative Genetics and Genomics, Aarhus University, 8210 Aarhus, Denmark; [email protected] 3 Washington Center for Muscle Biology, Department of Animal Sciences, Washington State University, Pullman, WA 99163, USA; [email protected] 4 Muscle Biology and Animal Biologics, Animal and Dairy Science, University of Wisconsin-Madison, Madison, WI 53558, USA; [email protected] * Correspondence: [email protected] Simple Summary: Skeletal muscle mass is an important economic trait, and muscle development and growth is a crucial factor to supply enough meat for human consumption. Thus, understanding (candidate) genes regulating skeletal muscle development is crucial for understanding molecular genetic regulation of muscle growth and can be benefit the meat industry toward the goal of in- creasing meat yields. During the past years, significant progress has been made for understanding these mechanisms, and thus, we decided to write a comprehensive review covering regulators and (candidate) genes crucial for muscle development and growth in farm animals. Detection of these genes and factors increases our understanding of muscle growth and development and is a great help for breeders to satisfy demands for meat production on a global scale. Citation: Mohammadabadi, M.; Abstract: Farm-animal species play crucial roles in satisfying demands for meat on a global scale, Bordbar, F.; Jensen, J.; Du, M.; Guo, W.
    [Show full text]
  • Original Article
    Original Article Deletion of Rictor in Brain and Fat Alters Peripheral Clock Gene Expression and Increases Blood Pressure Katja Drägert, Indranil Bhattacharya, Giovanni Pellegrini, Petra Seebeck, Abdelhalim Azzi, Steven A. Brown, Stavroula Georgiopoulou, Ulrike Held, Przemyslaw Blyszczuk, Margarete Arras, Rok Humar, Michael N. Hall, Edouard Battegay, Elvira Haas Abstract—The mammalian target of rapamycin complex 2 (mTORC2) contains the essential protein RICTOR and is activated by growth factors. mTORC2 in adipose tissue contributes to the regulation of glucose and lipid metabolism. In the perivascular adipose tissue, mTORC2 ensures normal vascular reactivity by controlling expression of inflammatory molecules. To assess whether RICTOR/mTORC2 contributes to blood pressure regulation, we applied a radiotelemetry approach in control and Rictor knockout (RictoraP2KO) mice generated using adipocyte protein-2 gene promoter–driven CRE recombinase expression to delete Rictor. The 24-hour mean arterial pressure was increased in RictoraP2KO mice, and the physiological decline in mean arterial pressure during the dark period was impaired. In parallel, heart rate and locomotor activity were elevated during the dark period with a pattern similar to blood pressure changes. This phenotype was associated with mild cardiomyocyte hypertrophy, decreased cardiac natriuretic peptides, and their receptor expression in adipocytes. Moreover, clock gene expression was reduced or phase-shifted in perivascular adipose tissue. No differences in clock gene expression were observed in the master clock suprachiasmatic nucleus, although Rictor gene expression was also lower in brain of RictoraP2KO mice. Thus, this study highlights the importance of RICTOR/mTORC2 for interactions between vasculature, adipocytes, and brain to tune physiological outcomes, such as blood pressure and locomotor activity.
    [Show full text]
  • Adenovirus-Mediated Interference of FABP4 Regulates Mrna Expression of ADIPOQ, LEP and LEPR in Bovine Adipocytes
    Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes S. Wei1,3, L.S. Zan1,2, H.B. Wang1, G. Cheng1, M. Du3, Z. Jiang3, G.J. Hausman4, D.C. McFarland5 and M.V. Dodson3 1College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China 2National Beef Cattle Improvement Center, Yangling, Shaanxi, P.R. China 3Department of Animal Sciences, Washington State University, Pullman, WA, USA 4United States Department of Agriculture, Agriculture Research Services, Athens, GA, USA 5Department of Animal Science, South Dakota State University, Brookings, SD, USA Corresponding authors: L.S. Zan / M.V. Dodson E-mail: [email protected] / [email protected] Genet. Mol. Res. 12 (1): 494-505 (2013) Received May 9, 2012 Accepted September 13, 2012 Published January 4, 2013 DOI http://dx.doi.org/10.4238/2013.January.4.21 ABSTRACT. Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (AD-FABP4), and then detected its influence on mRNA expression of four differentiation-related genes (PPARᵧ, CEBPA, CEBPB, and SREBF1) and three lipid metabolism-related genes (ADIPOQ, LEP and LEPR) of adipocytes. The FABP4 mRNA content, derived from bovine adipocytes, decreased by 41% (P < 0.01) after 24 h and 66% (P < 0.01) Genetics and Molecular Research 12 (1): 494-505 (2013) ©FUNPEC-RP www.funpecrp.com.br shRNA interference of FABP4 in bovine adipocytes 495 after 72 h of AD-FABP4 infection.
    [Show full text]
  • Fatty Acid Metabolism and the Basis of Brown Adipose Tissue Function
    ADIPOCYTE 2016, VOL. 5, NO. 2, 98–118 http://dx.doi.org/10.1080/21623945.2015.1122857 REVIEW Fatty acid metabolism and the basis of brown adipose tissue function Marıa Calderon-Domingueza,b, Joan F. Mira,b, Raquel Fuchoa,b, Mineia Webera,b, Dolors Serraa,b, and Laura Herreroa,b aDepartment of Biochemistry and Molecular Biology, School of Pharmacy, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Barcelona, Spain; bCIBER Fisiopatologıa de la Obesidad y la Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain ABSTRACT ARTICLE HISTORY Obesity has reached epidemic proportions, leading to severe associated pathologies such as insulin Received 13 August 2015 resistance, cardiovascular disease, cancer and type 2 diabetes. Adipose tissue has become crucial Revised 13 October 2015 due to its involvement in the pathogenesis of obesity-induced insulin resistance, and traditionally Accepted 12 November 2015 white adipose tissue has captured the most attention. However in the last decade the presence and KEYWORDS activity of heat-generating brown adipose tissue (BAT) in adult humans has been rediscovered. BAT brown adipose tissue; fatty decreases with age and in obese and diabetic patients. It has thus attracted strong scientific acid oxidation; lipid interest, and any strategy to increase its mass or activity might lead to new therapeutic approaches metabolism; obesity to obesity and associated metabolic diseases. In this review we highlight the mechanisms of fatty acid uptake, trafficking and oxidation in brown fat thermogenesis. We focus on BAT’s morphological and functional characteristics and fatty acid synthesis, storage, oxidation and use as a source of energy.
    [Show full text]
  • Novel Molecular Regulators of Adipose Tissue Metabolism
    Minerva Foundation Institute University of Helsinki for Medical Research NOVEL MOLECULAR REGULATORS OF ADIPOSE TISSUE METABOLISM Raghavendra Mysore Minerva Foundation Institute for Medical Research and Faculty of Medicine, Department of Internal Medicine and Doctoral program in Biomedicine, University of Helsinki, Finland ACADEMIC DISSERTATION To be presented for public examination with the permission of the Faculty of medicine, University of Helsinki in Lecture room 2, Haartman Institute, Haartmaninkatu 3, Helsinki on May 18th, 2018, at 12 noon Supervisors: Professor Vesa Olkkonen, Ph.D Minerva Foundation Institute for Medical Research, Helsinki, Finland University of Helsinki, Helsinki, Finland And Nidhina Haridas P A, Ph.D Minerva foundation institute for medical research Biomedicum, Helsinki, Finland Reviewers Jacob Hansen, Ph.D Associate professor, Department of Biology University of Copenhagen, Denmark And Tarja Kokkola, PhD Docent, University of Eastern Finland Opponent Professor Jussi Pihlajamäki, MD, PhD Professor of Clinical Nutrition. Clinical Nutrition and Obesity Center, Kuopio University Hospital, Kuopio, Finland Custos Professor Hannele Yki-Jarvinen (MD, FRCP) Professor of medicine Department of medicine, University of Helsinki, Finland Cover image: Comprehensive representation of thesis study illustrating several screenings (miRNA and gene expression) from obese and lean human adipose tissue and cultured adipocytes. Lipid droplet staining of 3T3-L1 adipocytes with Bodipy (green) and nuclei with DAPI (blue). ISBN 978-951-51-4251-1 (paperback) ISBN 978-951-51-4252-8 (PDF) To my teachers Thank you for your trust and inspiration Teacher is an absolute representation of Brahma (god of creation), Vishnu (god of Sustenance) and Shiva (god of annihilation). He creates, sustains knowledge and destroys the weeds of ignorance.
    [Show full text]
  • Targeting Circulating Adipocyte Protein 2 in the Context of Type 1 Diabetes Incidence and Pathogenesis
    Targeting Circulating Adipocyte Protein 2 in the Context of Type 1 Diabetes Incidence and Pathogenesis The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Robertson, Lauren Tyler. 2018. Targeting Circulating Adipocyte Protein 2 in the Context of Type 1 Diabetes Incidence and Pathogenesis. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:41128498 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use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©!EFGH!/)0'&+!1.2&'!3*-&'(%*+! "22!'$84(%!'&%&'A&#I!! 9$%%&'()($*+!"#A$%*'J!9'I!KLM4)+!<I!7*()6$%2$8$2C!=49C!D9! /)0'&+!1.2&'!3*-&'(%*+! ! "#$%&'()%!*($+,-#'()%!./(01+2'&!3$1'&()!4!()!'5&!*1)'&6'!17!"20&!8!9(#:&'&;!
    [Show full text]
  • The Effect of Overfeeding and Obesity on Canine Adipose Tissue and Skeletal Muscle Transcriptomes by Ryan Walter Grant Disserta
    THE EFFECT OF OVERFEEDING AND OBESITY ON CANINE ADIPOSE TISSUE AND SKELETAL MUSCLE TRANSCRIPTOMES BY RYAN WALTER GRANT DISSERTATION Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Nutritional Sciences in the Graduate College of the University of Illinois at Urbana-Champaign, 2011 Urbana, Illinois Doctoral Committee: Professor Sharon M. Donovan, Chair Associate Professor Kelly S. Swanson, Director of Research Associate Professor Thomas K. Graves Associate Professor Manabu T. Nakamura Abstract Overweight dogs have a reduced life expectancy and increased risk of chronic disease. During obesity development, adipose tissue undergoes major expansion and remodeling, but the biological processes involved are not well understood. The objective of study 1 was to analyze global gene expression profiles of adipose tissue in dogs, fed a high-fat (47% kcal/g) diet, during the transition from a lean to obese phenotype. Nine female beagles were randomized to ad libitum (n=5) feeding or body weight maintenance (n=4). Subcutaneous adipose tissue biopsy, skeletal muscle biopsy and blood samples were collected, and dual x-ray absorptiometry measurements were taken at 0, 4, 8, 12, and 24 wk of feeding. Ad libitum feeding increased (P<0.05) body weight, body fat mass, adipocyte size and serum leptin concentrations. Microarrays displayed 1,665 differentially expressed genes in adipose tissue over time in the ad libitum fed dogs. Alterations were observed in many homeostatic processes including metabolism, oxidative stress, mitochondrial homeostasis, and extracellular matrix. Our data implies that during obesity development subcutaneous adipose tissue has a large capacity for expansion, which is accompanied by tissue remodeling and short-term adaptations to the metabolic stresses associated with ad libitum feeding.
    [Show full text]
  • The Effect of Type 2 Diabetes Mellitus and Obesity on Muscle Progenitor Cell Function Shuzhi Teng* and Ping Huang*
    Teng and Huang Stem Cell Research & Therapy (2019) 10:103 https://doi.org/10.1186/s13287-019-1186-0 REVIEW Open Access The effect of type 2 diabetes mellitus and obesity on muscle progenitor cell function Shuzhi Teng* and Ping Huang* Abstract In addition to its primary function to provide movement and maintain posture, the skeletal muscle plays important roles in energy and glucose metabolism. In healthy humans, skeletal muscle is the major site for postprandial glucose uptake and impairment of this process contributes to the pathogenesis of type 2 diabetes mellitus (T2DM). A key component to the maintenance of skeletal muscle integrity and plasticity is the presence of muscle progenitor cells, including satellite cells, fibroadipogenic progenitors, and some interstitial progenitor cells associated with vessels (myo-endothelial cells, pericytes, and mesoangioblasts). In this review, we aim to discuss the emerging concepts related to these progenitor cells, focusing on the identification and characterization of distinct progenitor cell populations, and the impact of obesity and T2DM on these cells. The recent advances in stem cell therapies by targeting diabetic and obese muscle are also discussed. Keywords: Satellite cell, muscle progenitor cell, T2DM, Obesity Background A major metabolic defect associated with T2DM is the The incidence of overweight has been increasing dramatic- failure of proper glucose utilization by peripheral tissues ally worldwide due to increased uptake of high-calorie food, such as skeletal muscle and adipose tissue, the primary lack of physical activity, and genetic predisposition. Accord- targets of insulin-stimulated glucose uptake. In healthy ingly, obesity and its associated metabolic diseases such as humans, 70–80% of glucose uptake occurs in skeletal type 2 diabetes mellitus (T2DM) have become an epidemic muscle in the postprandial state through both health threat and economic burden [1].
    [Show full text]
  • Molecular and Cellular Mechanisms of Energy Homeostasis in Birds
    Molecular and cellular mechanisms of energy homeostasis in birds Yang Xiao Dissertation submitted to the Graduate Faculty of the Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Animal and Poultry Sciences Elizabeth R. Gilbert, Chair Mark A. Cline Sally E. Johnson Dongmin Liu Paul B. Siegel March, 2020 Blacksburg, VA Key Words: chicks, anorexia, obesity, adipose tissue, DNA methylation Copyright 2020, Yang Xiao Molecular and cellular mechanisms of energy homeostasis in birds Yang Xiao Abstract (Academic) Hypothalamus and adipose tissue are essential central and peripheral sites regulating energy homeostasis. Disruption of energy homeostasis can lead to diseases like anorexia and obesity in humans and reduced productivity in animals. Therefore, integrating knowledge in hypothalamic appetite regulation and adipose tissue metabolism is essential to maintain homeostasis. The aim of this dissertation was to elucidate molecular and cellular mechanisms of energy homeostasis in birds. We determined adipose tissue physiological changes during the first two weeks post-hatch in chickens from lines selected for low (LWS) and high (HWS) body weight. LWS was more dependent on yolk and subcutaneous fat mobilization for growth from hatch to day 4 post-hatch, with hyperplasia-predominated replenishment of the reservoir. In contrast, HWS was more dependent on feed for growth and maintained depot mass through hyperplasia and hypertrophy. From day 4 to 14 post-hatch, compared to maintenance of depot weight and adipocyte size in LWS, HWS accumulated clavicular and abdominal fat with minimal lipolysis. There was greater expression of precursor and proliferation markers in LWS with more apoptotic cells in the abdominal stromal vascular fraction on day 14 post-hatch, suggesting that apoptosis contributed to lower adipogenic potential and lack of abdominal fat in LWS.
    [Show full text]
  • Universidade Estadual De Campinas Faculdade De Ciências Médicas Higor Campos Do Nascimento Proteína Transportadora De Ácidos
    UNIVERSIDADE ESTADUAL DE CAMPINAS FACULDADE DE CIÊNCIAS MÉDICAS HIGOR CAMPOS DO NASCIMENTO PROTEÍNA TRANSPORTADORA DE ÁCIDOS GRAXOS DE ADIPÓCITOS (A-FABP): SUAS RELAÇÕES COM OBESIDADE E CITOCINAS PRÓ-INFLAMATÓRIAS EM PACIENTES COM CÂNCER DE MAMA CAMPINAS 2016 HIGOR CAMPOS DO NASCIMENTO PROTEÍNA TRANSPORTADORA DE ÁCIDOS GRAXOS DE ADIPÓCITOS (A-FABP): SUAS RELAÇÕES COM OBESIDADE E CITOCINAS PRÓ-INFLAMATÓRIAS EM PACIENTES COM CÂNCER DE MAMA Dissertação apresentada à Faculdade de Ciências Médicas da Universidade Estadual de Campinas como parte dos requisitos exigidos para a obtenção do título de Mestre em Ciências Médicas, na área de concentração em Ciências Biomédicas ORIENTADOR: Profª Drª Sílvia de Barros Mazon ESTE EXEMPLAR CORRESPONDE À VERSÃO FINAL DA DISSERTAÇÃO DEFENDIDA PELO ALUNO HIGOR CAMPOS DO NASCIMENTO, E ORIENTADO PELA PROFª. DRª. SÍLVIA DE BARROS MAZON. CAMPINAS 2016 BANCA EXAMINADORA DA DEFESA DE MESTRADO HIGOR CAMPOS DO NASCIMENTO ORIENTADORA: PROF.ª DR.ª SÍLVIA DE BARROS MAZON MEMBROS: 1. PROF.ª DR.ª SÍLVIA DE BARROS MAZON 2. PROF. DR. LÁZARO ALESSANDRO SOARES NUNES 3. PROF.ª DR.ª DENISE ENGELBRECHT ZANTUT WITTMANN Programa de Pós-Graduação em Ciências Médicas da Faculdade de Ciências Médicas da Universidade Estadual de Campinas. A ata de defesa com as respectivas assinaturas dos membros da banca examinadora encontra-se no processo de vida acadêmica do aluno. Data: 13/01/2016 Dedico este trabalho... A todas as mulheres que foram acometidas pelo câncer de mama e que, com força e coragem, travam uma batalha diária para sobreviverem e superarem essa doença que aflige tantas mulheres pelo mundo. AGRADECIMENTOS Primeiramente agradeço a Deus, meu Pai celestial, que com Sua bondade e misericórdia infinitas permitiu com que eu chegasse até aqui.
    [Show full text]
  • Contribution of a First-Degree Family History of Diabetes to Increased Serum Adipocyte Fatty Acid Binding Protein Levels Indepen
    OPEN International Journal of Obesity (2016) 40, 1649–1654 www.nature.com/ijo ORIGINAL ARTICLE Contribution of a first-degree family history of diabetes to increased serum adipocyte fatty acid binding protein levels independent of body fat content and distribution XHu1,XPan1,XMa1, Y Luo1,YXu1, Q Xiong1, Y Xiao2, Y Bao1 and W Jia1 BACKGROUND/OBJECTIVES: First-degree relatives of patients with diabetes bear an increased risk of diabetes, overweight/obesity and cardiovascular disease. Accumulating evidence indicates that circulating concentrations of adipokines are altered in individuals with a first-degree family history of diabetes (FHD), but the adipokine adipocyte fatty acid binding protein (A-FABP) has been rarely studied in this population. The present study explored the association between a first-degree FHD and serum A-FABP levels. SUBJECTS/METHODS: A total of 1962 normoglycemic participants were divided into subgroups of men, premenopausal women and postmenopausal women. Serum A-FABP levels were measured using a sandwich enzyme-linked immunoabsorbent assay. Abdominal fat distribution, including visceral fat area and subcutaneous fat area, was assessed by magnetic resonance imaging. RESULTS: Totals of 792 men, 544 premenopausal women and 626 postmenopausal women were enrolled. Serum A-FABP levels were much higher in subjects with a first-degree FHD than in those without an FHD in all subgroups (all Po0.05). Logistic regression analysis revealed an independent and positive relationship between a first-degree FHD and serum A-FABP levels in men (P = 0.029), premenopausal women (P = 0.036) and postmenopausal women (P = 0.008). Multiple stepwise regression analysis showed that a first-degree FHD was an independent factor positively associated with serum A-FABP levels in men (standardized β = 0.068, P = 0.029), premenopausal women (standardized β = 0.090, P = 0.018) and postmenopausal women (standardized β = 0.102, P = 0.004).
    [Show full text]