Cranial Nerves Expressing GFP in the MAPT-GFP Transgenic 3.5 Day Old Embryo

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Cranial Nerves Expressing GFP in the MAPT-GFP Transgenic 3.5 Day Old Embryo

2010 Table of Abbreviations ac: anterior commissure bmn: branchio-motor nuclei cb: cerebellum dt: dorsal thalamus (aka. thalamus) dvdt: dorso-ventral diencephalic tract e: epiphysis fc: facial nerve fg: facial ganglion hci: hindbrain commissural interneurons hg: hindbrain glia ht: hypothalamus LL: lateral line nerve llf: lateral/dorsal-longitudinal fasciculus mlf: medial-longitudinal fasciculus n: nasal pit nmlf: nucleus of the medio-longitudinal fasciculus npc: nucleus of the posterior commissure npoc: nucleus of the post-optic commissure oc: optic chiasma on: optic nerve pc: posterior commissure poc: post-optic commissure r: rhombomere rb: Rohon-Beard neurons sm: stria medullaris (habenular afferents) sma: spinal motor axons sot: supra-optic tract soc: supra-optic commissure t: telencephalon tc: optic tectum td: trochlear decussation tg: trigeminal ganglion tgt: tegmentum tptc: tract of the posterior tubercular commissure tvc: tegmental ventral commissure vg: vagal nerve vt: ventral thalamus (aka. pre-thalamus) Specification of brain territories inside the neural plate (all pictures are oriented anterior to the left) • Forebrain (F in red) and midbrain (M green) progenitors bud stage 1 somite stage F M F M Dorsal view F F Lateral view • Anterior and posterior diencephalic precursors (red) lie rostral to the midbrain anlage (green) bud stage 1 somite stage F F F F • The presumptive telencephalon (horse shoe in red) lies along the anterior border of the neural plate. The medial red domain is the hypothalamus and the anterior diencephalon is in green. rhl2 a B + zl e F • Summary schematic of the fate of the neural plate territories. Telencephalon (TEL) Hypothalamus (Hypo) TEL Prethalamu (Pth) Th Thalamus (Th) Pth Pretectum Epithalamus Pineal gland Hypo. Basal diencephalon Neurogenesis in 24hpf brain: - Detection of post-mitotic neurons using anti-Hu (elav) antibody. Dorsal view, rostral to the left. Axonal tracts in 28 hpf CNS: - Detection using anti-acetylated tubulin antibody. Axonal tracts in 48hpf CNS: Detection using anti-acetylated tubulin antibody. DAB revelation (secondary IgG, HRP conjugate) pc soc poc CB: Cerebellum.; Hb: Habenula; OB: Olfactory Bulb; OE: Olfactory Epithelium; OT: Optic Tectum; TEL: Telencephalon In red, acetylated tubulin staining and in green GFP in various transgenic lines. Photo Gallery - Axon detection using anti-acetylated tubulin antibody (secondary IgG, Alexa Fluor conjugate). Confocal stack. - Cranial nerves expressing GFP in the MAPT-GFP transgenic 3.5 day old embryo. - Dorsal view, anterior to the left, of a 3 day old dlx4/6GFP transgenic embryo showing dlx4 and dlx6 expressing cells in green and axons in red (anti-tubulin staining) - Lateral views of the trunk of a 30hpf MAPT-GFP transgenic embryo. GFP in green and acetylated tubulin in red. - Ventral views, anterior to the top of wild-type (A) and ace/fgf8-/- (B) 3 day old embryos. Arrow indicates misrouted axons. .

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