First Evidence of the Occurrence of Turnip Mosaic Virus in Ukraine and Molecular Characterization of Its Isolate
Received: 7 September 2017 | Accepted: 1 March 2018 DOI: 10.1111/jph.12703 ORIGINAL ARTICLE First evidence of the occurrence of Turnip mosaic virus in Ukraine and molecular characterization of its isolate Oleksiy Shevchenko1 | Ryosuke Yasaka2,3 | Olha Tymchyshyn1 | Tetiana Shevchenko1 | Kazusato Ohshima2,3 1Virology Department, ESC “Institute of Biology and Medicine”, Taras Shevchenko Abstract National University of Kyiv, Kyiv, Ukraine A total of 54 samples of Brassicaceae crops showing symptoms of mosaic, mottling, 2 Laboratory of Plant Virology, Department vein banding and/or leaf deformation were collected in Kyiv region (northern central of Applied Biological Sciences, Faculty of Agriculture, Saga University, Saga, Japan part of Ukraine) in 2014–2015. A half of collected samples was found to be infected 3The United Graduate School of Agricultural with Turnip mosaic virus (TuMV), and TuMV was detected in samples from Brassica Sciences, Kagoshima University, Kagoshima, oleracea var. capitata (cabbage), Raphanus sativus, Brassica juncea, Raphanus sp., Japan Sinapis alba, Camelina sativa and Bunias orientalis (weed). The full- length sequence of Correspondence the genomic RNA of a Ukrainian isolate (UKR9), which was isolated from cabbage, O. Shevchenko, Virology Department, ESC “Institute of Biology and Medicine”, Taras was determined. Recombination analysis of UKR9 isolate showed that this isolate Shevchenko National University of Kyiv, was an interlineage recombinant of world- Brassica and Asian- Brassica/Raphanus phy- Kyiv, Ukraine.
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