Supplemental Table 1. Full annotation of all differentially expressed genes between IS tissue of Day 7.5 pregnant Il15+/+ and Il15-/- mice as determined by microarray analysis.

log2(Fold‐ Chromosome change Probe Symbol Description Chromosome GenBank Gene Cytoband UniGene PubMed Gene Ontology Pathway P.Adjusted Location Il15+/+/Il1 5‐/‐)

XM_901690, XM_903949, XM_922038 molecular_function XM_901690, 1110038B RIKEN cDNA ‐35087182, ‐ 1990400 17 XM_903949, 68763 17 17 B1 Mm.28895 14 ‐0.593333 0.00134 12Rik 1110038B12 gene 35087182 XM_922038 XM_901690, XM_903949, XM_922038 cellular_component

biological_process molecular_function

cellular_component 1500015O RIKEN cDNA 3130368 1 43787446 NM_024283 78896 1 1 C1 Mm.50109 14 extracellular region ‐0.753333 0.001134 10Rik 1500015O10 gene biological_process

cytoplasmic vesicle molecular_function

cellular_component 2010011I RIKEN cDNA 1.03E+08 2 172171076 67017 2 2 H3 Mm.30013 12 biological_process WT Only WT Only 20Rik 2010011I20 gene membrane

integral to membrane molecular_function

cellular_component

RIKEN cDNA nucleus 2500003 1.07E+08 2500003M10 3 ‐90302879 66511 3 3 F2 Mm.154136 17 ‐0.543333 0.000797 M10Rik gene transcription

biological_process

regulation of transcription molecular_function 2510016G RIKEN cDNA 1.02E+08 7 72438 7 Mm.394279 7 cellular_component ‐0.576667 0.003076 02Rik 2510016G02 gene biological_process molecular_function 2900046B RIKEN cDNA 2630725 9 74327 9 Mm.5264 7 cellular_component 0.613333 0.00033 09Rik 2900046B09 gene biological_process molecular_function 2900046B RIKEN cDNA 4810324 9 74327 9 Mm.5264 7 cellular_component 0.623333 0.000638 09Rik 2900046B09 gene biological_process molecular_function 5031414D RIKEN cDNA 4850494 14 75415833 NM_198642 271221 14 14 D3 Mm.320280 8 cellular_component ‐0.666667 0.004576 18Rik 5031414D18 gene biological_process molecular_function 6330403K RIKEN cDNA 2060504 11 ‐70845442 NM_134022 103712 11 11 B4 Mm.27768 13 cellular_component WT Only WT Only 07Rik 6330403K07 gene biological_process molecular_function RIKEN cDNA 6720422 1.05E+08 6720422M22 3 99946 Mm.440672 7 cellular_component KO Only KO Only M22Rik gene biological_process molecular_function 9130230L RIKEN cDNA Mm.436542 1450315 5 ‐66379166 XR_035361 231253 5 5 C3.1 9 cellular_component 0.78 0.008423 23Rik 9130230L23 gene Mm.478504 biological_process molecular_function 9130230L RIKEN cDNA Mm.436542 2190452 5 ‐66379166 XR_035361 231253 5 5 C3.1 9 cellular_component 1.17667 0.002484 23Rik 9130230L23 gene Mm.478504 biological_process molecular_function 9330182O RIKEN cDNA 1.02E+08 15 328531 15 15 B3.1 Mm.60703 7 cellular_component 0.643333 0.007724 14Rik 9330182O14 gene biological_process molecular_function A030010E RIKEN cDNA 1.06E+08 9 320039 9 Mm.208296 7 cellular_component 0.696667 0.003566 16Rik A030010E16 gene biological_process molecular_function A430005L RIKEN cDNA 4200273 4 153331345 NM_175287 97159 4 4 E2 Mm.7066 14 cellular_component ‐0.543333 0.005176 14Rik A430005L14 gene biological_process nucleotide binding

ATP binding lysosome

lysosome

endosome

late endosome

Golgi apparatus

Golgi apparatus ATP‐binding transport cassette, sub‐ ABC 1660110 Abca5 11 ‐110130682 NM_147219 217265 11 11 E1 Mm.41942 14 WT only WT Only family A (ABC1), transporters negative regulation of macrophage derived member 5 foam cell differentiation

membrane

integral to membrane

ATPase activity

nucleoside‐triphosphatase activity

cholesterol efflux

high‐density lipoprotein particle remodeling

reverse cholesterol transport molecular_function

NM_016703*, carboxylesterase activity NM_021304, NR_003522 cellular_component abhydrolase NM_016703*, 31252438, 6220685 Abhd1 domain 5 NM_021304, 57742 5 5 B1 Mm.389615 13 biological_process ‐0.656667 0.004949 31252478 containing 1 NR_003522 NM_016703*, membrane NM_021304, NR_003522 integral to membrane

hydrolase activity carboxylesterase activity

response to wounding

abhydrolase membrane 4850504 Abhd2 domain 7 86418151 NM_018811 54608 7 7 D2 Mm.365490 14 0.596667 0.000561 containing 2 integral to membrane

hydrolase activity negative regulation of cell migration Fatty acid molecular_function metabolism

Valine, leucine and mitochondrion isoleucine degradation

acetyl‐Coenzyme Biosynthesis 6100750 Acaa1b A acyltransferase 9 ‐119057160 NM_146230 235674 9 9 F3 Mm.379402 13 of ‐0.503333 0.001556 biological_process 1B unsaturated fatty acids

Metabolic pathways

PPAR signaling pathway

Peroxisome Acap1 molecular_function

phospholipase C activity

signal transducer activity

cellular_component

lipid metabolic process

signal transduction ArfGAP with NM_153788, ARF GTPase activator activity coiled‐coil, XR_032069 2120093 0.65 0.004127 ankyrin repeat NM_153788, biological_process and PH domains 1 XR_032069 zinc ion binding

lipid catabolic process

hydrolase activity

intracellular signaling pathway

regulation of ARF GTPase activity metal ion binding Purine in utero embryonic development metabolism

Metabolic trophectodermal cell differentiation pathways

Primary purine nucleoside binding immunodefi ciency

liver development

placenta development

germinal center B cell differentiation

positive regulation of germinal center formation negative regulation of leukocyte migration negative regulation of leukocyte migration

negative regulation of mature B cell apoptosis

adenosine deaminase activity

adenosine deaminase activity

adenosine deaminase activity

adenosine deaminase activity

extracellular space

cytoplasm

cytoplasm

plasma membrane

adenosine catabolic process

adenosine catabolic process

adenosine catabolic process

deoxyadenosine catabolic process deoxyadenosine catabolic process

cell adhesion

nucleotide metabolic process

purine ribonucleoside monophosphate biosynthetic process

positive regulation of heart rate

membrane

hydrolase activity

deaminase activity

cell junction adenosine 2 94.0 cM 2 3130075 Ada 2 ‐163552319 NM_007398 11486 Mm.388 117 1.41667 0.017497 deaminase H3 lung development

positive regulation of B cell proliferation

dendrite cytoplasm

positive regulation of T cell differentiation in the thymus

neuronal cell body

negative regulation of apoptosis

regulation of T cell differentiation

positive regulation of T cell differentiation

positive regulation of smooth muscle contraction

dATP catabolic process

dATP catabolic process

adenosine metabolic process

hypoxanthine biosynthetic process

inosine biosynthetic process

inosine biosynthetic process

inosine biosynthetic process xanthine biosynthetic process

positive regulation of alpha‐beta T cell differentiation

metal ion binding

lung alveolus development

Peyer's patch development

embryonic digestive tract development

negative regulation of inflammatory response

negative regulation of inflammatory response

positive regulation of calcium‐mediated signaling

positive regulation of T cell receptor signaling pathway

positive regulation of T cell activation

negative regulation of penile erection

negative regulation of thymocyte apoptosis

negative regulation of mucus secretion

negative regulation of mucus secretion metalloendopeptidase activity

extracellular region

proteinaceous extracellular matrix

proteolysis

integrin‐mediated signaling pathway a disintegrin‐like and metabolic process NM_011782, metallopeptidase XM_001480027 5890592 Adamts5 (reprolysin type) peptidase activity 0.836667 0.002307 NM_011782, with XM_001480027 thrombospondin metallopeptidase activity type 1 motif, 5 metallopeptidase activity zinc ion binding

hydrolase activity

extracellular matrix

metal ion binding adenosine receptor activity, G‐protein coupled

adenosine receptor activity, G‐protein coupled

positive regulation of chronic inflammatory response to non‐antigenic stimulus

signal transducer activity

receptor activity

G‐protein coupled receptor activity

G‐protein coupled receptor activity

plasma membrane

plasma membrane

plasma membrane

signal transduction

G‐protein coupled receptor protein signaling pathway

activation of adenylate cyclase activity by G‐ protein signaling pathway

positive regulation of cell proliferation

negative regulation of cell proliferation

cell surface

positive regulation vascular endothelial growth factor production

NM_007413, positive regulation of norepinephrine adenosine A2b XM_001473907 secretion 6860253 Adora2b ‐0.546667 0.002398 receptor NM_007413, XM_001473907 positive regulation of steroid biosynthetic process membrane

integral to membrane

positive regulation of cAMP biosynthetic process

positive regulation of cAMP biosynthetic process

positive regulation of cGMP biosynthetic process

positive regulation of guanylate cyclase activity

cellular response to extracellular stimulus positive regulation of chemokine production

positive regulation of interleukin‐6 production negative regulation of collagen biosynthetic process

positive regulation of catecholamine secretion positive regulation of mast cell degranulation

regulation of angiogenesis

positive regulation of vasodilation

relaxation of vascular smooth muscle nucleotide binding

protein kinase activity

protein serine/threonine kinase activity

G‐protein coupled receptor kinase activity

G‐protein coupled receptor kinase activity

signal transducer activity

ATP binding nucleus

protein amino acid phosphorylation adrenergic Olfactory ‐113389583, ‐ 1.03E+08 Adrbk2 receptor kinase, 5 NM_001035531 320129 5 60.0 cM 5 F Mm.285619 30 protein amino acid phosphorylation transductio 0.553333 0.001944 113339497 beta 2 n signal transduction

kinase activity

phosphorylation

phosphorylation

transferase activity

axon

dendritic spine

dendritic shaft

synapse

beta‐adrenergic receptor kinase activity Purine nucleotide binding metabolism

Alanine, aspartate magnesium ion binding and glutamate metabolism

Metabolic adenylosuccinate synthase activity pathways

adenylosuccinate synthase activity adenylosuccinate 4850132 Adssl1 12 113858257 NM_007421 11565 12 12 F1 Mm.3440 22 protein binding 0.74 0.001474 synthetase like 1 GTP binding

cytoplasm

cytoplasm

purine nucleotide metabolic process

purine nucleotide biosynthetic process membrane

ligase activity

metal ion binding molecular_function

actin binding

calcium ion binding

cytoplasm

allograft cytoskeleton 3520372 Aif1l inflammatory 2 31805822 NM_145144 108897 2 2 B Mm.24838 14 0.446667 0.000292 factor 1‐like plasma membrane

biological_process

membrane

cell projection

protein complex

Pentose and glucuronate aldehyde reductase activity interconver sions

Fructose and aldehyde reductase activity mannose metabolism

Galactose aldehyde reductase activity metabolism

Glycerolipid extracellular space metabolism aldo‐keto reductase family 6 13.0 cM 6 Mm.389126 1.02E+08 Akr1b3 6 ‐34253929 NM_009658 11677 50 0.686667 0.006489 1, member B3 B1 Mm.451 Pyruvate (aldose reductase) cytoplasm metabolism

Metabolic sorbitol biosynthetic process pathways

metabolic process response to organic substance

oxidoreductase activity

stress‐activated protein kinase signaling cascade

positive regulation of JAK‐STAT cascade

positive regulation of smooth muscle cell proliferation

oxidation reduction aldehyde reductase activity

cytoplasm

aldo‐keto mitochondrion 3120288 Akr1b8 reductase family NM_008012 1.12333 0.000229 1, member B8 oxidoreductase activity

cellular lipid metabolic process

oxidation reduction positive regulation of protein amino acid phosphorylation

cytoplasm

plasma membrane

plasma membrane NM_010241, NM_011250*, transport XM_910009* thymoma viral NM_010241, proto‐oncogene 1 apoptosis 6350575 Aktip 8 ‐93647397 NM_011250*, 14339 8 41.0 cM 8 D Mm.324588 26 ‐1.05 0.007276 interacting XM_910009* protein protein transport NM_010241, NM_011250*, membrane XM_910009* small conjugating protein ligase activity

positive regulation of protein binding

post‐translational protein modification

regulation of protein metabolic process receptor binding

vascular endothelial growth factor receptor binding 2 84.0 cM 2 extracellular region 510750 Angpt4 angiopoietin 4 2 151737067 NM_009641 11602 Mm.189536 14 ‐0.693333 0.003512 G3 extracellular space

signal transduction

endoderm development calcium ion binding

14 13.0 cM 14 4780022 Anxa8 annexin A8 14 34899175 NM_013473 11752 Mm.3267 12 calcium‐dependent phospholipid binding 1.13333 0.000853 B

blood coagulation Glycine, leukocyte migration involved in inflammatory serine and response threonine metabolism

positive regulation of acute inflammatory Tyrosine response metabolism

Phenylalani positive regulation of leukocyte migration ne metabolism

beta‐ copper ion binding Alanine metabolism

Metabolic extracellular space pathways

microsome

response to stress amine oxidase, 6840129 Aoc3 copper containing 11 101191919 NM_009675 11754 11 11 B2‐B5 Mm.67281 35 0.713333 0.007128 cell adhesion 3 primary amine oxidase activity

primary amine oxidase activity

regulation of blood pressure

amine metabolic process

positive regulation of glucose transport

membrane integral to membrane

oxidoreductase activity

eating behavior

protein homodimerization activity

metal ion binding

quinone binding

oxidation reduction aldehyde oxidase activity

cellular_component

metabolic process

electron carrier activity aldehyde oxidase 1 23.2 cM 1 4180592 Aox3 1 58169979 NM_023617 71724 Mm.20108 15 electron carrier activity 0.666667 0.001659 3 C1.3 molybdenum ion binding

metal ion binding

iron‐sulfur cluster binding

2 iron, 2 sulfur cluster binding nucleotide binding

GTP binding

GTP binding

GTP binding

intracellular

nucleus

cytoplasm

ADP‐ribosylation Golgi apparatus 5690725 Arl3 19 ‐46605598 NM_019718 56350 19 19 D1 Mm.22085 21 ‐1.93333 0.002435 factor‐like 3 cytoskeleton

cell cycle

small GTPase mediated signal transduction

membrane GDP binding

metal ion binding

cell division Glycosamin catalytic activity oglycan degradation

Metabolic N‐acetylgalactosamine‐4‐sulfatase activity pathways

arylsulfatase activity Lysosome

mitochondrion

lysosome

lysosome

rough endoplasmic reticulum

Golgi apparatus

autophagy 13 50.0 cM 13 Mm.300178 1400451 Arsb arylsulfatase B 13 94541633 NM_009712 11881 38 0.53 0.00111 C3‐D1 Mm.472255 central nervous system development

response to nutrient

metabolic process

metabolic process

sulfuric ester hydrolase activity

sulfuric ester hydrolase activity

response to pH

hydrolase activity

response to estrogen stimulus

metal ion binding

response to methylmercury Lysine molecular_function degradation Tight DNA binding junction

protein binding

cellular_component

nucleus

tight junction

transcription ash1 (absent, small, or 1.03E+08 Ash1l 3 88769733 NM_138679 192195 3 3 F1 Mm.130752 16 biological_process ‐1.85667 0.005341 homeotic)‐like (Drosophila) methyltransferase activity

zinc ion binding

chromatin modification

transferase activity

histone‐lysine N‐methyltransferase activity

cell junction

regulation of transcription

metal ion binding Oxidative molecular_function phosphoryla tion

Metabolic cellular_component pathways

transport Phagosome

Collecting ion transport duct acid secretion

biological_process ATPase, H+ transporting, 4200458 Atp6v0e2 6 48487567 NM_133764 76252 6 6 B3 Mm.30214 12 hydrogen ion transmembrane transporter ‐0.87 0.001773 lysosomal V0 activity subunit E2 ATP synthesis coupled proton transport

ATP hydrolysis coupled proton transport proton transport

membrane

integral to membrane

hydrolase activity

proton‐transporting V‐type ATPase, V0 domain RNA binding

RNA binding

binding

protein binding

nucleus

nucleus

cytoplasm

adult locomotory behavior Mm.342683 13 28.0 cM 13 5550156 Atxn1 ataxin 113‐45650261 NM_009124 20238 Mm.342686 69 visual learning 0.876667 0.002484 A5 Mm.476983 nuclear matrix

negative regulation of phosphorylation

nuclear inclusion body

negative regulation of insulin‐like growth factor receptor signaling pathway

positive regulation of transcription from RNA polymerase II promoter

regulation of excitatory postsynaptic membrane potential actin binding

protein binding

cytoplasm

cytoskeleton 5690452 Avil advillin 10 126437764 NM_009635 11567 10 10 D3 Mm.10739 20 WT Only WT Only response to stress

cytoskeleton organization actin filament organization

actin filament capping molecular_function

cellular_component NM_001077237, NM_145511, biological_process XR_005082 NM_001077237, zinc ion binding 1980164 BC003331 NM_145511, ‐1.33667 0.00136 XR_005082 membrane NM_001077237, NM_145511, integral to membrane XR_005082 oxidoreductase activity

oxidation reduction molecular_function cDNA sequence 12 10.93 cM 7050397 BC022687 12 114047185 NM_145450 217887 Mm.186678 13 cellular_component 0.96 0.01004 BC022687 12 F1 biological_process molecular_function NM_001025431, protein binding BTB (POZ) domain 138082319, NM_145534 4200746 Btbd3 2 228662 2 2 F3 Mm.153405 14 ‐0.793333 0.01181 containing 3 138104228 NM_001025431, cellular_component NM_145534 biological_process cysteine‐type endopeptidase activity

calcium‐dependent cysteine‐type endopeptidase activity

intracellular 4050706 Capn5 calpain 57‐105270074 NM_007602 12337 7 7 E2 Mm.326847 19 0.6 0.003476 proteolysis

peptidase activity

cysteine‐type peptidase activity

hydrolase activity molecular_function

cellular_component coiled‐coil domain 1170008 Ccdc126 6 49269351 NM_175098 57895 6 6 B2.3 Mm.32416 14 0.526667 0.00133 containing 126 extracellular region

biological_process molecular_function coiled‐coil domain coiled‐coil domain 6840463 Ccdc163 4 116381534 NM_026714 68394 4 4 D1 Mm.425201 11 cellular_component ‐0.72 0.004428 containing 163 biological_process Cytokine‐ cytokine cytokine activity receptor interaction

‐41719767, ‐ NM_001048179, Chemokine Mm.335946 chemokine (C‐C 41719767, ‐ NM_011336 4 13.3 cM 4 protein binding signaling 2120070 Ccl27a 4 20301 Mm.425176 25 0.88 0.002583 motif) ligand 27A 41716350, ‐ NM_001048179, A5 pathway Mm.463309 41716342 NM_011336 Intestinal immune biological_process network for IgA production protein binding

plasma membrane 16 29.0 cM 16 5260647 Cd200 CD200 antigen 16 ‐45382247 NM_010818 17470 Mm.245851 47 membrane ‐0.59 0.002447 A1 integral to membrane

identical protein binding neuron migration

cyclin‐dependent protein kinase activity

calcium ion binding

protein binding

membrane fraction

nucleus

nucleus

cytoplasm

cytoplasm

plasma membrane

protein amino acid phosphorylation

neuron cell‐cell adhesion

muscarinic acetylcholine receptor signaling pathway axon guidance

axonal fasciculation

brain development

cytoskeletal protein binding

embryo development

membrane

membrane

kinase activity

cyclin‐dependent kinase activity kinase 5, 11 46.5 cM 11 Mm.142275 Alzheimer's 1.05E+08 Cdk5r1 11 80290547 NM_009871 12569 97 0.63 0.008516 regulatory B5 Mm.474282 cyclin‐dependent protein kinase 5 disease subunit 1 (p35) holoenzyme complex

cyclin‐dependent protein kinase 5 holoenzyme complex

cyclin‐dependent protein kinase 5 activator activity

cyclin‐dependent protein kinase 5 activator activity

cyclin‐dependent protein kinase 5 activator activity

neuron differentiation

axon

dendrite

growth cone

neuron projection development

neuromuscular junction

ionotropic glutamate receptor signaling pathway

positive regulation of phosphorylation

neuronal cell body

dendritic spine contractile fiber

positive regulation of neuron apoptosis

protein serine/threonine kinase activator activity

cadherin binding

regulation of catalytic activity negative regulation of transcription from RNA polymerase II promoter

protein kinase inhibitor activity

cyclin‐dependent protein kinase inhibitor activity

protein binding

cyclin‐dependent nucleus 7 69.49 cM 7 6520577 Cdkn1c kinase inhibitor 7 ‐150644246 NM_009876 12577 Mm.168789 197 Cell cycle ‐0.673333 0.005163 F5 1C (P57) nucleus

cell cycle

cell cycle arrest

kinase activity

negative regulation of phosphorylation

neuron maturation receptor activity NM_001039185, plasma membrane NM_001039186, NM_001039187, signal transduction NM_011926 NM_001039185, membrane NM_001039186, carcinoembryonic NM_001039187, integral to membrane antigen‐related NM_011926 7100176 Ceacam1 7 ‐26246720 26365 7 5.5 cM 7 A3 Mm.322502 80 ‐0.856667 0.004637 cell adhesion NM_001039185, integral to membrane molecule 1 NM_001039186, NM_001039187, protein kinase binding NM_011926 NM_001039185, NM_001039186, positive regulation of MAP kinase activity NM_001039187, NM_011926 interspecies interaction between organisms molecular_function cellular_component ChaC, cation cytoplasm transport 7100725 Chac1 2 119176977 NM_026929 69065 2 2 E5 Mm.35083 12 0.833333 0.000707 regulator‐like 1 (E. apoptosis coli) response to unfolded protein

biological_process molecular_function

nucleus

nucleolus

cytokine induced cytoplasm 2900114 Ciapin1 apoptosis 8 ‐97343718 NM_134141 109006 8 8 C5 Mm.27582 19 1.05667 0.000982 inhibitor 1 cytoplasm

apoptosis

anti‐apoptosis

hemopoiesis Cell adhesion structural molecule activity molecules (CAMs)

Tight plasma membrane junction

Leukocyte 4150270 Cldn15 claudin 15 5 137443738 NM_021719 60363 5 5 G2 Mm.87202 23 1.44 0.001631 transendoth tight junction elial migration

membrane

integral to membrane

cell junction Cell adhesion structural molecule activity molecules (CAMs)

Tight protein binding junction

Leukocyte transendoth plasma membrane elial migration tight junction 5 75.0 cM 5 2570524 Cldn3 claudin 3 5 135462083 NM_009902 12739 Mm.158662 46 ‐0.546667 0.002038 G2 tight junction

tight junction

membrane

integral to membrane

integral to membrane

calcium‐independent cell‐cell adhesion

cell junction

identical protein binding extracellular region

anti‐apoptosis

cell death

positive regulation of cell proliferation NM_013492, XM_001475611 aggresome 5420075 Clu Clusterin ‐0.486667 0.00111 NM_013492, XM_001475611 endocrine pancreas development

positive regulation of cell differentiation

perinuclear region of cytoplasm

neuron projection morphogenesis Amino sugar and nucleus nucleotide sugar metabolism

Metabolic nucleus pathways

cytidine CMP‐N‐acetylneuraminate biosynthetic monophospho‐N‐ 6 74.0 cM 6 process 2190129 Cmas 6 142705205 NM_009908 12764 Mm.3820 20 ‐0.896667 0.011059 acetylneuraminic G3 acid synthetase N‐acylneuraminate cytidylyltransferase activity

N‐acylneuraminate cytidylyltransferase activity lipopolysaccharide biosynthetic process

transferase activity

nucleotidyltransferase activity molecular_function

cytokine activity

cellular_component CKLF‐like MARVEL extracellular space transmembrane 6200037 Cmtm8 9 ‐114698462 NM_027294 70031 9 9 F3 Mm.150064 11 ‐0.503333 0.002882 domain chemotaxis containing 8 biological_process

membrane

integral to membrane nucleic acid binding

protein binding

nucleus

nucleus CCR4‐NOT transcription transcription RNA 2450338 Cnot7 8 ‐41578389 NM_011135 18983 8 8 A4 Mm.272551 27 0.536667 0.003659 complex, subunit degradation 7 transcription activator activity

CCR4‐NOT complex

regulation of transcription

positive regulation of transcription from RNA polymerase II promoter Focal extracellular matrix structural constituent adhesion

ECM‐ protein binding receptor interaction

extracellular region Amoebiasis

proteinaceous extracellular matrix collagen, type I, 6 0.68 cM 6 380364 Col1a2 6 4455696 NM_007743 12843 Mm.277792 176 ‐1.49667 0.004164 alpha 2 A1 collagen

collagen collagen type I

SMAD binding

platelet‐derived growth factor binding

protein heterotrimerization collagen, type VIII, 430020 Col8a2 ‐0.89 0.002038 alpha 2

Ubiquinone and other prenyltransferase activity terpenoid‐ quinone biosynthesis

Metabolic prenyltransferase activity pathways

cellular_component

coenzyme Q2 mitochondrion homolog, 4230037 Coq2 5 ‐101083744 NM_027978 71883 5 5 E3 Mm.260661 14 ‐0.736667 0.003152 prenyltransferase mitochondrion (yeast) ubiquinone biosynthetic process

ubiquinone biosynthetic process

isoprenoid biosynthetic process

biosynthetic process

membrane

integral to membrane

transferase activity

Ubiquinone and other molecular_function terpenoid‐ quinone biosynthesis

Metabolic monooxygenase activity pathways

mitochondrion

ubiquinone biosynthetic process coenzyme Q6 2760162 Coq6 12 85702917 NM_172582 217707 12 12 D1 Mm.280062 13 ‐0.62 0.004203 homolog (yeast) biological_process

metabolic process

oxidoreductase activity

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen

FAD binding

oxidation reduction immunological synapse

actin binding

protein binding

cytoplasm

cytoskeleton

plasma membrane

calcium ion transport

actin filament organization

regulation of actin polymerization or depolymerization

cytoskeletal protein binding

regulation of cell shape

actin cytoskeleton

membrane

coronin, actin cell migration 7 62.5 cM 7 3140609 Coro1a binding protein 7 ‐133843287 NM_009898 12721 Mm.290482 42 Phagosome 1.28 0.000301 F3 1A positive regulation of cell migration

leukocyte chemotaxis

regulation of actin filament polymerization

cell leading edge cytoplasmic vesicle

uropod organization

response to cytokine stimulus

positive regulation of T cell proliferation

identical protein binding

T cell homeostasis

homeostasis of number of cells within a tissue

positive regulation of T cell activation

actin filament binding immunological synapse

actin binding

protein binding

cytoplasm

cytoskeleton

plasma membrane

calcium ion transport

actin filament organization

regulation of actin polymerization or depolymerization

cytoskeletal protein binding

regulation of cell shape

actin cytoskeleton

membrane

coronin, actin cell migration 7 62.5 cM 7 3190037 Coro1a binding protein 7 ‐133843287 NM_009898 12721 Mm.290482 42 Phagosome 0.9 0.002012 F3 1A positive regulation of cell migration

leukocyte chemotaxis

regulation of actin filament polymerization cell leading edge

cytoplasmic vesicle

uropod organization

response to cytokine stimulus

positive regulation of T cell proliferation

identical protein binding

T cell homeostasis

homeostasis of number of cells within a tissue

positive regulation of T cell activation

actin filament binding immunological synapse

actin binding

protein binding

cytoplasm

cytoskeleton

plasma membrane

calcium ion transport

actin filament organization

regulation of actin polymerization or depolymerization

cytoskeletal protein binding

regulation of cell shape

actin cytoskeleton

membrane

coronin, actin cell migration 7 62.5 cM 7 3190020 Coro1a binding protein 7 ‐133843287 NM_009898 12721 Mm.290482 42 Phagosome 1.24333 0.000203 F3 1A positive regulation of cell migration

leukocyte chemotaxis regulation of actin filament polymerization

cell leading edge

cytoplasmic vesicle

uropod organization

response to cytokine stimulus

positive regulation of T cell proliferation

identical protein binding

T cell homeostasis

homeostasis of number of cells within a tissue

positive regulation of T cell activation

actin filament binding protein binding cysteine‐rich C‐ 2320040 Crct1 3 ‐92818127 NM_028798 74175 3 3 F1 Mm.35806 11 cellular_component 0.98 6.70E‐05 terminal 1 biological_process DNA binding

sequence‐specific DNA binding transcription factor activity

nucleus

nucleus

transcription

regulation of transcription, DNA‐dependent

cysteine‐serine‐ apoptosis 1230053 Csrnp1 rich nuclear 9 ‐119880283 NM_153287 215418 9 9 F4 Mm.477982 16 1.63 0.012242 protein 1 post‐embryonic development

regulation of transcription

positive regulation of transcription from RNA polymerase II promoter platelet‐derived growth factor receptor signaling pathway

skeletal system morphogenesis

palate development

face morphogenesis binding

protein binding

nucleus catenin, beta like 2 94.0 cM 2 5900750 Ctnnbl1 2 157563136 NM_025680 66642 Mm.45193 18 Spliceosome ‐0.603333 0.002075 1 H2 nucleus

apoptosis

induction of apoptosis Neuroactive ligand‐ catalytic activity receptor interaction

serine‐type endopeptidase activity Lysosome

Renin‐ intermediate filament angiotensin system

proteolysis Amoebiasis

Systemic lupus proteolysis erythemato sus

heparin binding

peptidase activity

peptidase activity

serine‐type peptidase activity

14 20.5 cM 14 membrane 1190711 Ctsg cathepsin G14‐56718717 NM_007800 13035 Mm.4858 40 WT Only WT Only C3 hydrolase activity

stored secretory granule

response to lipopolysaccharide response to lipopolysaccharide

negative regulation of growth of symbiont in host

negative regulation of growth of symbiont in host

positive regulation of immune response

positive regulation of immune response

defense response to Gram‐positive bacterium

defense response to fungus

defense response to fungus

neutrophil mediated killing of gram‐positive bacterium cysteine‐type endopeptidase activity

proteolysis

360368 Ctsw cathepsin W19 ‐5465239 NM_009985 13041 19 19 A Mm.113590 11 peptidase activity Lysosome WT Only WT Only

cysteine‐type peptidase activity

hydrolase activity molecular_function CUE domain 87912647, 1660463 Cuedc1 11 NM_198013 103841 11 11 C Mm.374846 12 cellular_component 0.53 0.002321 containing 1 87983066 biological_process molecular_function

copper ion binding NM_001113562, cutC copper NM_025530 110452 Cutc transporter 19 43827512 66388 19 19 C3 Mm.20257 13 cellular_component ‐0.54 0.004088 NM_001113562, homolog (E.coli) NM_025530 biological_process

copper ion homeostasis molecular_function

cellular_component cell wall GPI anchor biosynthetic process biogenesis 43 C‐ 3800722 Cwh43 5 73797316 NM_181323 231293 5 5 C3.2 Mm.280211 10 0.7 0.000721 terminal homolog biological_process (S. cerevisiae) membrane integral to membrane monooxygenase activity

iron ion binding

endoplasmic reticulum

microsome

electron carrier activity

cytochrome P450, membrane family 4, 1660176 Cyp4f18 XM_001472253 ‐0.666667 0.000965 subfamily f, integral to membrane polypeptide 18 oxidoreductase activity

heme binding

metal ion binding

leukotriene‐B4 20‐monooxygenase activity

oxidation reduction protein binding

cytoplasm

cytohesin 1 cytoplasm 1780408 Cytip interacting 2 ‐57981549 NM_139200 227929 2 2 C1.1 Mm.479941 16 0.813333 0.006902 protein endosome

cell cortex

regulation of cell adhesion DNA segment, Chr Mm.22506 D17H6S56 17 19.0 cM 17 4760095 17, human 17 110956 Mm.385081 17 ‐1.12667 0.001772 E‐5 B1 D6S56E 5 Mm.431805 molecular_function RIKEN cDNA D930007N 1.03E+08 D930007N19 17 106823 Mm.437429 7 cellular_component ‐0.813333 0.009117 19Rik gene biological_process cytoplasm

arginine metabolic process

arginine catabolic process

dimethylargininase activity dimethylarginine 4480551 Ddah2 dimethylaminohy 17 35196045 NM_016765 51793 17 17 B1 Mm.1457 17 dimethylargininase activity ‐0.7 0.000309 drolase 2 amino acid binding

hydrolase activity

hydrolase activity, acting on carbon‐nitrogen (but not peptide) bonds, in linear amidines

Sphingolipid sphingosine hydroxylase activity metabolism

Metabolic cellular_component pathways

endoplasmic reticulum

lipid metabolic process

fatty acid biosynthetic process

sphinganine metabolic process

degenerative sphinganine metabolic process spermatocyte ‐109928431, ‐ 1170056 Degs2 homolog 2 12 NM_027299 70059 12 12 F1 Mm.207605 15 lipid biosynthetic process 1.54667 0.000245 109925002 (Drosophila), lipid desaturase membrane

integral to membrane

oxidoreductase activity

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

sphingolipid biosynthetic process

sphingolipid delta‐4 desaturase activity

ceramide biosynthetic process

oxidation reduction Steroid catalytic activity biosynthesis

Metabolic membrane fraction pathways

nucleus

cytoplasm endoplasmic reticulum

Golgi apparatus

cytosol

cytoskeleton

steroid biosynthetic process

cholesterol biosynthetic process

cholesterol biosynthetic process

cholesterol biosynthetic process

Ras protein signal transduction

protein localization

steroid metabolic process 24‐ 840600 Dhcr24 dehydrocholester 4 106233642 NM_053272 74754 4 4 C7 Mm.133370 26 cholesterol metabolic process ‐1.73 0.018863 ol reductase negative regulation of cell proliferation

lipid biosynthetic process

tissue development

membrane

integral to membrane

cellular membrane organization

sterol metabolic process

sterol biosynthetic process

oxidoreductase activity

oxidoreductase activity, acting on the CH‐CH group of donors, NAD or NADP as acceptor

male genitalia development

plasminogen activation

amyloid precursor protein catabolic process FAD binding

oxidation reduction guanyl‐nucleotide exchange factor activity

Rho guanyl‐nucleotide exchange factor activity

protein binding

protein binding

intracellular

dynamin binding cytoplasm 6900403 Dnmbp 19 ‐43922456 NM_028029 71972 19 19 D1 Mm.159024 16 ‐0.6 0.00541 protein Golgi apparatus

cytoskeleton

intracellular signaling pathway

cell junction

regulation of Rho protein signal transduction

synapse Rho guanyl‐nucleotide exchange factor activity ‐121941260, ‐ dedicator of 14 67.0 cM 14 cellular_component 5080037 Dock9 14 121941260, ‐ NM_001081039 105445 Mm.441054 19 0.556667 0.004928 cytokinesis 9 E5 121941260 Rho GTPase binding

regulation of catalytic activity MAPKKK cascade

transmembrane receptor protein tyrosine kinase signaling protein activity

transmembrane receptor protein tyrosine kinase docking protein activity 14 39.0 cM 14 5340273 Dok2 docking protein 2 14 71174187 NM_010071 13449 Mm.243323 27 0.79 0.00111 D2‐D3 insulin receptor binding

cellular_component

transmembrane receptor protein tyrosine kinase signaling pathway

Ras protein signal transduction molecular_function diamine oxidase‐ 1.05E+08 Doxl2 6 48925141 NM_001029987 243376 6 6 B2.3 Mm.244112 9 cellular_component 1.09667 0.001686 like protein 2 biological_process nuclease activity

endonuclease activity eosinophil‐ associated, ribonuclease activity 2260292 Ear11 ribonuclease A 14 ‐51874936 NM_053113 93726 14 14 C1 Mm.153688 5 2.56667 0.000158 family, member cellular_component 11 metabolic process

hydrolase activity response to hypoxia

metalloendopeptidase activity

membrane fraction

Golgi apparatus

plasma membrane

proteolysis

proteolysis

apoptosis

peptidase activity

peptidase activity

metallopeptidase activity

endothelin external side of plasma membrane Mm.401062 3940551 Ece1 converting 4 137418151 NM_199307 230857 4 4 D3 39 0.583333 0.001738 Mm.439998 enzyme 1 cell surface

membrane

integral to membrane

protein processing

peptide hormone processing

hydrolase activity

stored secretory granule vesicle

positive regulation of G‐protein coupled receptor protein signaling pathway

metal ion binding

pharyngeal system development RNA binding

translation initiation factor activity

binding

protein binding eukaryotic NM_172703, protein binding translation XM_001477622 670707 Eif4g3 0.786667 0.001742 initiation factor 4 NM_172703, cellular_component gamma XM_001477622 translation

regulation of translation

biological_process

RNA metabolic process signal transducer activity

receptor activity

transmembrane receptor activity

G‐protein coupled receptor activity

calcium ion binding

plasma membrane EGF, latrophilin seven signal transduction 3120086 Eltd1 transmembrane 3 151100845 NM_133222 170757 3 3 H3‐H4 Mm.317261 9 ‐0.75 0.004637 domain cell surface receptor linked signaling pathway containing 1

G‐protein coupled receptor protein signaling pathway

neuropeptide signaling pathway

membrane

integral to membrane protein dimerization activity protein binding

extracellular region

proteinaceous extracellular matrix

EMI domain proteinaceous extracellular matrix 360400 Emid2 5 ‐137217633 NM_024474 140709 5 5 G1 Mm.295020 15 1.07 0.002413 containing 2 endoplasmic reticulum

Golgi apparatus

positive regulation of cell‐substrate adhesion molecular_function endogenous 3130338 Erv3 retroviral 2 ‐131679414 71995 2 2 F1 Mm.22 8 cellular_component 0.703333 0.005163 sequence 3 biological_process exocyst

molecular_function

cellular_component

cytoplasm

plasma membrane Insulin exocyst complex 3060037 Exoc7 11 ‐116149311 NM_016857 53413 11 11 E2 Mm.22530 19 signaling ‐0.7 0.008744 component 7 transport pathway

exocytosis

biological_process

protein transport

membrane Glycosamin oglycan Golgi membrane biosynthesis ‐ heparan sulfate

Metabolic mesoderm formation pathways

endoplasmic reticulum

endoplasmic reticulum

endoplasmic reticulum membrane Golgi apparatus

Golgi apparatus

metabolic process

metabolic process

acetylglucosaminyltransferase activity

heparan sulfate proteoglycan biosynthetic process

glucuronosyltransferase activity

membrane exostoses 2 51.0 cM 2 6520239 Ext2 2 ‐93535787 NM_010163 14043 Mm.4336 28 ‐0.5 0.001215 (multiple) 2 E1 integral to membrane

transferase activity

transferase activity, transferring glycosyl groups

transferase activity, transferring hexosyl groups

cell differentiation

intrinsic to endoplasmic reticulum membrane

heparan sulfate N‐ acetylglucosaminyltransferase activity

protein homodimerization activity

glucuronosyl‐N‐acetylglucosaminyl‐ proteoglycan 4‐alpha‐N‐ acetylglucosaminyltransferase activity

glucuronosyl‐N‐acetylglucosaminyl‐ proteoglycan 4‐alpha‐N‐ acetylglucosaminyltransferase activity

N‐acetylglucosaminyl‐proteoglycan 4‐beta‐ glucuronosyltransferase activity

N‐acetylglucosaminyl‐proteoglycan 4‐beta‐ glucuronosyltransferase activity molecular_function family with sequence ‐35024825, ‐ 6590086 Fam118b 9 NM_175411 109229 9 9 A5.3 Mm.24197 11 cellular_component ‐0.776667 0.003198 similarity 118 35024549 similarity 118, 35024549 member B biological_process tRNA binding

nucleotide binding

molecular_function

aminoacyl‐tRNA ligase activity

phenylalanine‐tRNA ligase activity

ATP binding

cellular_component phenylalanyl‐ Aminoacyl‐ 2650593 Farsa tRNA synthetase, 8 87380884 66590 8 8 C3 Mm.292517 16 tRNA 1.15667 0.000652 cytoplasm alpha subunit biosynthesis translation

tRNA aminoacylation for protein translation

phenylalanyl‐tRNA aminoacylation

biological_process

ligase activity

tRNA aminoacylation 2480059 Fbln1 fibulin 1 1.38667 0.000985 calcium ion binding

protein binding

NM_001081437, extracellular region NM_007992 Mm.249146 3440215 Fbln2 fibulin 2 6 91162757 14115 6 37.2 cM 6 D 37 1.26 0.000336 NM_001081437, Mm.410235 proteinaceous extracellular matrix NM_007992 positive regulation of cell‐substrate adhesion

extracellular matrix binding molecular_function F‐box and WD‐40 3170037 Fbxw9 8 87584017 NM_026791 68628 8 8 C3 Mm.374815 13 cellular_component 0.55 0.002484 domain protein 9 biological_process molecular_function

protein binding

cellular_component FCH domain only FCH domain only 1240411 Fcho1 8 ‐74232285 NM_028715 74015 8 8 B3.3 Mm.22025 13 intracellular protein transport WT Only WT Only 1 biological_process

vesicle‐mediated transport

clathrin adaptor complex molecular_function

cellular_component FGFR1 oncogene 60538 Fgfr1op2 6 146526432 NM_026218 67529 6 6 G3 Mm.333499 14 WT Only WT Only partner 2 cytoplasm

biological_process DNA binding

sequence‐specific DNA binding transcription factor activity

nucleus

transcription 13 17.0 cM 13 4850088 Foxq1 forkhead box Q1 13 31650038 NM_008239 15220 Mm.44235 32 0.976667 0.00226 A3.2 regulation of transcription, DNA‐dependent

hair follicle morphogenesis

sequence‐specific DNA binding

regulation of transcription ion channel activity

chloride channel activity

transport FXYD domain‐ containing ion Mm.263847 2320450 Fxyd3 7 ‐31855553 NM_008557 17178 7 7 B1 19 ion transport ‐0.963333 0.003149 transport Mm.389870 regulator 3 membrane

integral to membrane

chloride channel complex osteoblast differentiation

signal transducer activity

receptor activity

G‐protein coupled receptor activity

GABA‐B receptor activity GABA‐B receptor activity

protein binding

soluble fraction

cytoplasm

endoplasmic reticulum membrane

microsome

plasma membrane

signal transduction

G‐protein coupled receptor protein signaling pathway

negative regulation of adenylate cyclase activity

synaptic vesicle

negative regulation of cell proliferation

regulation of glutamate secretion gamma‐ Neuroactive aminobutyric acid 17 20.4 cM 17 negative regulation of gamma‐aminobutyric ligand‐ 1.02E+08 Gabbr1 17 37182910 54393 Mm.32191 72 1 0.000965 (GABA) B B1 acid secretion receptor receptor, 1 interaction membrane

integral to membrane

synaptosome

cell junction

axolemma

regulation of cAMP biosynthetic process

mitochondrial membrane

negative regulation of epinephrine secretion

negative regulation of dopamine secretion

neuronal cell body

dendritic spine dendritic shaft

membrane raft

synapse

postsynaptic membrane

negative regulation of synaptic transmission

positive regulation of growth hormone secretion

growth arrest and nucleus NM_009010*, DNA‐damage‐ Gadd45gi NM_183358 Mm.255539 2970047 inducible, gamma 8 87356180 102060 8 8 C3 18 0.846667 0.001138 p1 NM_009010*, Mm.467206 interacting NM_183358 mitochondrion protein 1 cell cycle signal transducer activity

receptor activity

G‐protein coupled receptor activity

galanin receptor activity

galanin receptor activity

plasma membrane

plasma membrane

signal transduction

G‐protein coupled receptor protein signaling pathway Neuroactive 11 70.0 cM 11 Mm.477240 ligand‐ 6450739 Galr2 galanin receptor 2 11 116142252 NM_010254 14428 26 G‐protein coupled receptor protein signaling ‐0.466667 0.000985 E2 Mm.57149 receptor pathway interaction activation of adenylate cyclase activity by G‐ protein signaling pathway

elevation of cytosolic calcium ion concentration

membrane

integral to membrane neuron projection development

neuropeptide binding

inositol phosphate metabolic process

phosphatidylinositol metabolic process hydrolase activity, hydrolyzing O‐glycosyl Galactose compounds metabolism

Starch and alpha‐glucosidase activity sucrose metabolism

Metabolic cellular_component pathways glucosidase, 1.06E+08 Ganc 2 120229631 NM_172672 76051 2 2 F1 Mm.38851 11 0.54 0.003281 alpha; neutral C carbohydrate metabolic process

glucose metabolic process

metabolic process

metabolic process

hydrolase activity

hydrolase activity, acting on glycosyl bonds

Folate nucleotide binding biosynthesis

Metabolic GTP cyclohydrolase I activity pathways

GTP cyclohydrolase I activity

GTP cyclohydrolase I activity

calcium ion binding

GTP binding

GTP binding

soluble fraction

nucleus

cytoplasm protein complex assembly

tetrahydrobiopterin biosynthetic process

tetrahydrobiopterin biosynthetic process

metabolic process GTP 14 19.5 cM 14 670364 Gch1 14 ‐47773569 NM_008102 14528 Mm.10651 35 ‐1.29 0.000113 cyclohydrolase 1 C2‐3 regulation of blood pressure

zinc ion binding

regulation of lung blood pressure

hydrolase activity

GTP‐dependent protein binding

vasodilation

protein complex

negative regulation of blood pressure

tetrahydrofolate biosynthetic process

metal ion binding

response to pain

coenzyme binding

neuromuscular process controlling posture

dihydrobiopterin metabolic process

protein homooligomerization

protein heterooligomerization Folate nucleotide binding biosynthesis

Metabolic GTP cyclohydrolase I activity pathways

GTP cyclohydrolase I activity

GTP cyclohydrolase I activity

calcium ion binding GTP binding

GTP binding

soluble fraction

nucleus

cytoplasm

protein complex assembly

tetrahydrobiopterin biosynthetic process

tetrahydrobiopterin biosynthetic process

metabolic process GTP 14 19.5 cM 14 6550358 Gch1 14 ‐47773569 NM_008102 14528 Mm.10651 35 ‐1.03667 0.001773 cyclohydrolase 1 C2‐3 regulation of blood pressure

zinc ion binding

regulation of lung blood pressure

hydrolase activity

GTP‐dependent protein binding

vasodilation

protein complex

negative regulation of blood pressure

tetrahydrofolate biosynthetic process

metal ion binding

response to pain

coenzyme binding

neuromuscular process controlling posture

dihydrobiopterin metabolic process

protein homooligomerization

protein heterooligomerization glycerol metabolic process lipid metabolic process

phosphoric diester hydrolase activity glycerophosphodi glycerophosphodiester phosphodiesterase ester activity 6110292 Gdpd3 phosphodiesteras 7 133909927 NM_024228 68616 7 7 F4 Mm.246881 13 2.70667 0.001462 e domain membrane containing 3 integral to membrane

hydrolase activity

metal ion binding Alanine, aspartate glutamine‐fructose‐6‐phosphate and transaminase (isomerizing) activity glutamate metabolism

Amino sugar and glutamine‐fructose‐6‐phosphate nucleotide transaminase (isomerizing) activity sugar metabolism

glutamine Metabolic sugar binding fructose‐6‐ 11 26.0 cM 11 pathways 2370129 Gfpt2 11 49607656 NM_013529 14584 Mm.24402 18 0.713333 0.001462 phosphate B1.2 transaminase 2 cytoplasm

carbohydrate metabolic process

glutamine metabolic process

glutamine metabolic process

metabolic process

transaminase activity

carbohydrate biosynthetic process

transferase activity GINS complex nucleus 610576 Gins3 subunit 3 (Psf3 8 98157458 NM_030198 78833 8 8 D1 Mm.35546 12 0.626667 0.00273 homolog) DNA replication protein binding

protein binding GIPC PDZ domain 3800463 Gipc2 containing family, 3 ‐151756804 NM_016867 54120 3 3 H3 Mm.97 13 cellular_component 0.796667 0.001927 member 2 cytoplasm

biological_process cytoplasm

mitochondrion

transport

metabolic process

electron carrier activity 13 44.0 cM 13 3520471 Glrx glutaredoxin 13 75977333 NM_053108 93692 Mm.25844 30 ‐2.15 8.50E‐05 C1 protein disulfide oxidoreductase activity

protein‐disulfide reductase (glutathione) activity

electron transport chain

cell redox homeostasis molecular_function XM_001476682, predicted gene XM_001476875 1.01E+08 Gm3235 8 1E+08 8 8 C5 Mm.464228 cellular_component 0.553333 0.003359 3235 XM_001476682, XM_001476875 biological_process Oxidative phosphoryla tion

Cardiac muscle XM_001004846, contraction predicted XM_894265 1500142 Gm6969 12 629383 12 12 A1.3 Mm.390445 ‐1.22667 0.001475 pseudogene 6969 XM_001004846, Alzheimer's XM_894265 disease

Parkinson's disease

Huntington' s disease molecular_function predicted gene 1.04E+08 Gm949 18 47081468 NM_001033446 381142 18 18 C Mm.329423 9 cellular_component WT Only WT Only 949 biological_process nucleotide binding molecular_function

GTP binding guanine cellular_component nucleotide 6620022 Gnl2 XM_908539 1.26333 0.001247 binding protein‐ intracellular like 2 nucleus

biological_process

ribosome biogenesis Golgi membrane

receptor activity

Golgi apparatus

Golgi apparatus

cis‐Golgi network SNARE golgi SNAP transport interactions 730279 Gosr1 receptor complex 11 ‐76540103 NM_016810 53334 11 11 B5 Mm.20931 13 ‐0.773333 0.000371 in vesicular member 1 ER to Golgi vesicle‐mediated transport transport protein transport

membrane

integral to membrane

integral to membrane

vesicle‐mediated transport integrin binding

integral to plasma membrane

cell adhesion

heparin binding glycoprotein 6 21.0 cM 6 1780138 Gpnmb (transmembrane) 6 48986516 NM_053110 93695 Mm.302602 39 0.686667 0.00032 B2.3 membrane nmb integral to membrane

cytoplasmic membrane‐bounded vesicle

cytoplasmic vesicle molecular_function receptor activity

cellular_component G protein‐coupled 7 68.0 cM 7 4900754 Gpr123 7 147020072 NM_177469 52389 Mm.39863 14 plasma membrane 0.73 0.003003 receptor 123 F4 biological_process

membrane

integral to membrane

Glutathione temperature homeostasis metabolism

Arachidonic negative regulation of inflammatory response acid to antigenic stimulus metabolism

peroxidase activity

glutathione peroxidase activity

glutathione peroxidase activity

glutathione peroxidase activity glutathione 12 36.0 cM 12 Mm.441856 6940619 Gpx2 12 ‐77893321 NM_030677 14776 35 glutathione peroxidase activity ‐1.44333 0.001462 peroxidase 2 C3 Mm.57225 cytoplasm

cytosol

response to oxidative stress

metabolic process

metabolic process

response to symbiotic bacterium

oxidoreductase activity

interaction with symbiont

oxidation reduction nucleotide binding

DNA binding

protein kinase activity protein serine/threonine kinase activity

protein serine/threonine kinase activity

ATP binding

ATP binding

nucleus germ cell‐specific 11 47.0 cM 11 5270270 Gsg2 11 ‐72948986 NM_010353 14841 Mm.42045 19 nucleus 0.766667 0.004834 gene 2 B4 protein amino acid phosphorylation

protein amino acid phosphorylation

cell cycle

cell cycle

intracellular protein kinase cascade

kinase activity

chromatin modification

transferase activity

Glutathione glutathione transferase activity metabolism

Metabolism of xenobiotics cytoplasm by glutathione S‐ cytochrome 6650600 Gsto1 transferase 19 47929478 NM_010362 14873 19 19 D1 Mm.378931 22 P450 ‐0.553333 0.002855 omega 1 Drug metabolism ‐ metabolic process cytochrome P450

transferase activity

L‐ascorbic acid biosynthetic process

Glutathione glutathione transferase activity metabolism Metabolism of xenobiotics glutathione transferase activity by cytochrome P450

glutathione S‐ 10 40.9 cM 10 Mm.24118 Drug 1580519 Gstt2 transferase, theta 10 ‐75294589 NM_010361 14872 17 ‐0.733333 0.009157 B5‐C1 Mm.465331 metabolism ‐ 2 nucleus cytochrome P450

nucleus

cytoplasm

cytosol

glutathione metabolic process

transferase activity catalytic activity

serine‐type endopeptidase activity

proteolysis

1990112 Gzme Granzyme E NM_010373 peptidase activity WT Only WT Only

serine‐type peptidase activity

hydrolase activity

cytolysis catalytic activity

serine‐type endopeptidase activity

proteolysis 14 20.5 cM 14 6200008 Gzmf granzyme F14‐56824099 NM_010374 14943 Mm.457976 20 peptidase activity WT Only WT Only C3 serine‐type peptidase activity

hydrolase activity

cytolysis catalytic activity

serine‐type endopeptidase activity

proteolysis 14 20 5 cM 14 14 20.5 cM 14 1230341 Gzmg granzyme G14‐56775417 NM_010375 14944 Mm.14868 13 peptidase activity WT Only WT Only C3 serine‐type peptidase activity

hydrolase activity

cytolysis membrane

integral to membrane

transferase activity hyaluronan 15 31.2 cM 15 5360181 Has2 15 ‐56497181 NM_008216 15117 Mm.5148 41 transferase activity, transferring glycosyl ‐0.56 0.001927 synthase 2 D1 groups

transferase activity, transferring hexosyl groups

hyaluronan synthase activity Tight protein binding junction

Bacterial invasion of nucleus epithelial cells

cytoplasm

mitochondrion hematopoietic positive regulation of cell proliferation 7100309 Hcls1 cell specific Lyn 16 36935068 NM_008225 15163 16 16 B Mm.4091 25 0.64 0.003717 substrate 1 response to hormone stimulus

SH3 domain binding

SH3 domain binding

erythrocyte differentiation

protein complex binding

positive regulation of tyrosine phosphorylation of STAT protein molecular_function

catalytic activity HD domain 4230017 Hddc3 7 87488022 NM_026812 68695 7 7 D3 Mm.21171 12 ‐0.56 0.004428 containing 3 cellular_component

biological_process protein binding intracellular HECT, C2 and WW domain cytoplasm 13 8.0 cM 13 1.03E+08 Hecw1 containing E3 13 ‐14318704 NM_001081348 94253 Mm.125298 12 0.58 0.000391 A1 ubiquitin protein protein modification process ligase 1 ligase activity

acid‐amino acid ligase activity catalytic activity

binding

cytoplasm

lipid metabolic process

fatty acid metabolic process

prostaglandin metabolic process

hydroxyprostagla metabolic process ndin 6770192 Hpgd 8 58773348 NM_008278 15446 8 8 B3.2 Mm.18832 24 ‐0.496667 0.001863 dehydrogenase 15 metabolic process (NAD) 15‐hydroxyprostaglandin dehydrogenase (NAD+) activity

15‐hydroxyprostaglandin dehydrogenase (NAD+) activity

15‐hydroxyprostaglandin dehydrogenase (NAD+) activity

oxidoreductase activity

oxidation reduction organelle organization

blood coagulation

cytoplasmic membrane‐bounded vesicle Hermansky‐ Pudlak syndrome 19 42.0 cM 19 2510026 Hps1 19 ‐42829685 NM_019424 192236 Mm.218381 57 melanocyte differentiation ‐1.08 0.003568 1 homolog C3 (human) positive regulation of natural killer cell activation

secretion of lysosomal enzymes

pigmentation positive regulation of immunoglobulin production

positive regulation of humoral immune response mediated by circulating immunoglobulin

cellular_component

extracellular region

transport

hemoglobin metabolic process

3440114 Hpx hemopexin 7 ‐112740124 NM_017371 15458 7 7 F1 Mm.3485 17 heme binding ‐0.57 0.004164

heme metabolic process

positive regulation of tyrosine phosphorylation of Stat1 protein

metal ion binding

regulation of protein metabolic process

positive regulation of response to interferon‐ gamma

positive regulation of interferon‐gamma‐ mediated signaling pathway Calcium neural crest cell migration signaling pathway

Neuroactive positive regulation of endothelial cell ligand‐ proliferation receptor interaction

Gap G‐protein coupled receptor internalization junction

heart morphogenesis

cardiac muscle hypertrophy

signal transducer activity

receptor activity

G‐protein coupled receptor activity

serotonin receptor activity serotonin receptor activity

serotonin receptor activity

Ras GTPase activator activity

cytoplasm

plasma membrane

plasma membrane

plasma membrane

signal transduction

5‐ signal transduction hydroxytryptamin 6510538 Htr2b 1 ‐87995611 NM_008311 15559 1 1 C5 Mm.439747 59 0.943333 0.00142 e (serotonin) G‐protein coupled receptor protein signaling receptor 2B pathway

G‐protein coupled receptor protein signaling pathway

activation of protein kinase C activity by G‐ protein coupled receptor protein signaling pathway

activation of phospholipase C activity by serotonin receptor signaling pathway

heart development

drug binding

neural crest cell differentiation

membrane

integral to membrane

response to drug

negative regulation of apoptosis

embryonic morphogenesis

positive regulation of cytokine secretion

serotonin binding

serotonin binding positive regulation of cell division

ERK1 and ERK2 cascade

cellular response to temperature stimulus regulation of cell growth

catalytic activity

serine‐type endopeptidase activity

protein binding

protein binding

insulin‐like growth factor binding

extracellular region

HtrA serine proteolysis 3450601 Htra1 7 138079716 NM_019564 56213 7 7 F3 Mm.30156 26 0.846667 0.001679 peptidase 1 peptidase activity

serine‐type peptidase activity

hydrolase activity

growth factor binding

negative regulation of transforming growth factor beta receptor signaling pathway

negative regulation of BMP signaling pathway ion channel activity

voltage‐gated ion channel activity

transport

ion transport

response to pH

response to zinc ion NM_001042489, hydrogen voltage‐ 122659745, NM_028752 proton transport 460133 Hvcn1 5 74096 5 5 F Mm.28804 21 ‐0.77 0.002086 gated channel 1 122660306 NM_001042489, NM_028752 membrane

integral to membrane integral to membrane

voltage‐gated proton channel activity

regulation of ion transmembrane transport

transmembrane transport molecular_function

cellular_component

isoamyl acetate‐ lipid metabolic process hydrolyzing 1690309 Iah1 esterase 1 12 21322252 NM_026347 67732 12 12 A1.3 Mm.335830 11 biological_process ‐0.866667 0.002698 homolog (S. cerevisiae) lipid catabolic process

hydrolase activity

hydrolase activity, acting on ester bonds molecular_function

cellular_component

isoamyl acetate‐ lipid metabolic process hydrolyzing 2900170 Iah1 esterase 1 12 21322252 NM_026347 67732 12 12 A1.3 Mm.335830 11 biological_process ‐0.656667 0.005176 homolog (S. cerevisiae) lipid catabolic process

hydrolase activity

hydrolase activity, acting on ester bonds nucleotide binding

molecular_function

catalytic activity

aminoacyl‐tRNA ligase activity

isoleucine‐tRNA ligase activity

ATP binding NM_198653, XM_980542*, cytoplasm XR_031710 NM_198653, isoleucine‐tRNA mitochondrion 4760204 Iars2 XM_980542*, 0.71 0.003003 synthetase 2 XR_031710 mitochondrion NM_198653, XM_980542*, translation XR 031710 XR_031710

tRNA aminoacylation for protein translation

isoleucyl‐tRNA aminoacylation

biological_process

zinc ion binding

ligase activity extracellular region

plasma membrane intercellular adhesion cell adhesion molecule 4, 1.02E+08 Icam4 9 20833816 78369 9 8.0 cM 9 A3 Mm.30220 14 1.28 0.001901 Landsteiner‐ membrane Wiener blood group integral to membrane

cell‐cell adhesion Citrate cycle isocitrate dehydrogenase (NAD+) activity (TCA cycle)

Metabolic mitochondrion pathways

tricarboxylic acid cycle isocitrate 1.03E+08 Idh3b dehydrogenase 3 2 ‐130105047 170718 2 2 F3 Mm.29590 16 1.03667 0.000267 isocitrate metabolic process (NAD+) beta 2‐oxoglutarate metabolic process

NADH metabolic process

oxidoreductase activity

oxidation reduction ossification

insulin‐like growth factor receptor binding

hormone activity

protein binding

extracellular region NM_001122736, extracellular space NM_001122737, NM_010514 extracellular space ‐149836672 ‐ NM 001122736 ‐149836672, ‐ NM_001122736, insulin‐like 7 69.09 cM 7 6510020 Igf2 7 149836672, ‐ NM_001122737, 16002 Mm.3862 363 0.733333 0.002056 growth factor 2 F5 carbohydrate metabolic process 149836672 NM_010514 NM_001122736, glucose metabolic process NM_001122737, NM_010514 growth factor activity

organ morphogenesis

response to organic cyclic substance

exocrine pancreas development

response to drug

positive regulation of cell division activation of MAPK activity

humoral immune response mediated by circulating immunoglobulin

antigen binding

antigen binding

transmembrane receptor activity

protein binding

extracellular region

plasma membrane

external side of plasma membrane

membrane

integral to membrane

immunoglobulin immunoglobulin mediated immune response 360113 Ighg2c heavy constant XR_033948 KO Only KO Only gamma 2C B cell receptor complex

antigen processing and presentation

positive regulation of B cell proliferation

immunoglobulin complex, circulating

positive regulation of MAPKKK cascade early endosome to late endosome transport

positive regulation of endocytosis

perinuclear region of cytoplasm

positive regulation of peptidyl‐tyrosine phosphorylation

B cell receptor signaling pathway

positive regulation of B cell activation Mm.246497 Mm.304472 Mm.305376 Mm.313447 Mm.313465 Mm.313480 Mm.313488 Mm.335723 Mm.342177 Mm.342187 XM_001472091, Mm.351752 immunoglobulin XM_001474025 12 59.0 cM 12 Mm.390491 1690184 Igh‐VJ558 heavy chain (J558 12 16061 78 ‐3.06 0.000718 XM_001472091, F2 Mm.390523 family) XM_001474025 Mm.390541 Mm.423002 Mm.423936 Mm.425757 Mm.425779 Mm.427704 Mm.435577 Mm.436242 Mm.436336 Mm.436337 Mm.458003 XM_001476664, XM_001476679, XM_001476703 immunoglobulin XM_001476664, 6370309 Igkc kappa constant XM_001476679, ‐3.39667 0.000267 Gene XM_001476703 XM_001476664, XM_001476679, XM_001476703 XM_001476664, XM_001476679, XM_001476703 immunoglobulin XM_001476664, 1230347 Igkc kappa constant XM_001476679, ‐3.55 0.000267 Gene XM_001476703 XM_001476664, XM_001476679, XM_001476703 DNA binding

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

protein binding

nucleus

nucleus

transcription

regulation of transcription, DNA‐dependent

regulation of transcription, DNA‐dependent

interferon phagocytosis 8 65.0 cM 8 610161 Irf8 regulatory factor 8 123260275 NM_008320 15900 Mm.334861 112 1.47667 0.000707 E1 8 immune response

response to bacterium

myeloid cell differentiation

positive regulation of interferon‐gamma production

positive regulation of interleukin‐12 production

defense response to bacterium

defense response to protozoan

negative regulation of growth of symbiont in host regulation of transcription

positive regulation of transcription, DNA‐ dependent

cellular response to lipopolysaccharide Focal receptor activity adhesion

ECM‐ integrin binding receptor interaction

Cell adhesion basement membrane molecules (CAMs)

Hematopoie plasma membrane tic cell lineage

Regulation of actin cell‐cell adherens junction cytoskeleto n

Pathways in cell adhesion cancer

Small cell cell‐matrix adhesion lung cancer

Hypertrophi c integrin‐mediated signaling pathway cardiomyop athy (HCM)

Arrhythmog enic right integrin complex ventricular 2 38.0 cM 2 3830129 Itga6 integrin alpha 6 2 71625139 NM_008397 16403 Mm.225096 160 cardiomyop ‐0.716667 0.009117 C2‐C3 athy (ARVC)

Dilated integrin complex cardiomyop athy

external side of plasma membrane

cell surface membrane

integral to membrane

basolateral plasma membrane

cell‐cell adhesion

positive regulation of cell‐cell adhesion

cellular response to extracellular stimulus

cell adhesion mediated by integrin

odontogenesis of dentine‐containing tooth

laminin binding

filopodium assembly

brown fat cell differentiation

leukocyte migration

leukocyte migration angiogenesis

nucleic acid binding

DNA binding

DNA binding

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

protein binding Kruppel‐like 1.04E+08 Klf5 14 99697909 12224 14 14 E2.2 Mm.30262 53 WT Only WT Only factor 5 intracellular

nucleus

transcription

regulation of transcription, DNA‐dependent zinc ion binding

microvillus assembly

regulation of transcription

positive regulation of transcription

metal ion binding catalytic activity

serine‐type endopeptidase activity

serine‐type endopeptidase activity

proteolysis kallikrein 1‐ 7 23.11 cM 7 5900632 Klk1b27 related peptidase 7 51307659 NM_020268 16619 Mm.445911 8 proteolysis ‐0.57 0.00285 B4 b27 peptidase activity

peptidase activity

serine‐type peptidase activity

hydrolase activity catalytic activity

serine‐type endopeptidase activity

receptor signaling protein activity

proteolysis

kallikrein 1‐ signal transduction 7 23.03 cM 7 630348 Klk1b3 related peptidase 7 51453599 NM_008693 18050 Mm.439740 48 ‐0.52 0.002698 B4 b3 growth factor activity

peptidase activity

serine‐type peptidase activity

hydrolase activity

metal ion binding Antigen processing receptor activity and presentatio n Natural killer cell binding mediated killer cell lectin‐ cytotoxicity like receptor, 6 62.52 cM 6 3190114 Klrd1 6 129543505 NM_010654 16643 Mm.391515 39 WT Only WT Only subfamily D, F3 member 1 Graft‐versus‐ sugar binding host disease

external side of plasma membrane

membrane

integral to membrane killer cell lectin‐ like receptor 2360133 Klrg1 WT Only WT Only subfamily G, member 1 extracellular region keratinocyte 5220605 Krtdap differentiation 7 31572923 NM_001033131 64661 7 7 B1 Mm.268157 12 epidermis development 0.716667 0.008423 associated protein cell differentiation lysosome

lysosome

lysosomal membrane lysosomal‐ 5550372 Laptm5 associated protein 4 130469248 NM_010686 16792 4 4 D2.3 Mm.271868 18 integral to plasma membrane Lysosome 0.633333 0.000229 transmembrane 5 transport

membrane

integral to membrane leukotriene B4 receptor activity

signal transducer activity

receptor activity

G‐protein coupled receptor activity

leukotriene receptor activity

leukotriene receptor activity Neuroactive plasma membrane leukotriene B4 14 20.5 cM 14 ligand‐ 1770056 Ltb4r1 14 56384798 NM_008519 16995 Mm.480704 54 WT Only WT Only receptor 1 C3 receptor signal transduction interaction signal transduction

G‐protein coupled receptor protein signaling pathway

G‐protein coupled receptor protein signaling pathway

membrane

integral to membrane molecular_function

LY6/PLAUR cellular_component 1.01E+08 Lypd6 domain 2 49921981 NM_177139 320343 2 2 C1.1 Mm.130607 12 0.943333 0.001022 containing 6 extracellular region

biological_process

Glycerolipid carboxylesterase activity metabolism

Metabolic lipid binding 88674405, pathways monoglyceride 2030446 Mgll 6 88674697, NM_011844 23945 6 6 D1 Mm.272197 19 0.563333 0.001367 lipase 88674697 hydrolase activity

hydrolase activity

synapse

acylglycerol lipase activity

Glutathione glutathione transferase activity metabolism

Metabolism of xenobiotics glutathione transferase activity by cytochrome P450

Drug metabolism ‐ glutathione transferase activity cytochrome P450

nucleus

mitochondrion mitochondrion

mitochondrion

mitochondrial outer membrane microsomal 6020605 Mgst1 glutathione S‐ 6 138089057 NM_019946 56615 6 6 G1 Mm.14796 20 mitochondrial inner membrane ‐0.493333 0.002838 transferase 1 peroxisomal membrane

endoplasmic reticulum

endoplasmic reticulum

microsome

microsome

glutathione metabolic process

response to organic nitrogen

membrane

transferase activity

response to lipopolysaccharide

response to drug

identical protein binding

protein homodimerization activity

apical part of cell Leukocyte transendoth angiogenesis elial migration

GnRH ovarian follicle development signaling pathway

Pathways in response to hypoxia cancer

Bladder response to hypoxia cancer

blood vessel maturation

intramembranous ossification fibronectin binding

metalloendopeptidase activity

metalloendopeptidase activity

extracellular region

proteinaceous extracellular matrix

extracellular space

nucleus

cytoplasm

plasma membrane

proteolysis

proteolysis

response to oxidative stress

negative regulation of cell adhesion

metabolic process

metabolic process matrix 8 44.0 cM 8 peptidase activity 2570603 Mmp2 metallopeptidase 8 95351226 NM_008610 17390 Mm.29564 273 0.586667 0.000453 C5 2 peptidase activity

metallopeptidase activity

zinc ion binding

response to mechanical stimulus

axon regeneration in the peripheral nervous system

membrane

cell migration

hydrolase activity

sarcomere

collagen catabolic process collagen catabolic process

extracellular matrix

response to drug

response to hydrogen peroxide

positive regulation of apoptosis

response to estrogen stimulus

negative regulation of vasoconstriction

metal ion binding

skeletal system morphogenesis

tissue remodeling

face morphogenesis

bone trabecula formation

prostate gland epithelium morphogenesis

cellular response to estradiol stimulus metalloendopeptidase activity

calcium ion binding

extracellular region

proteinaceous extracellular matrix

extracellular space

nucleus

proteolysis

metabolic process

peptidase activity matrix 2650368 Mmp3 metallopeptidase 9 7445821 NM_010809 17392 9 1.0 cM 9 A1 Mm.4993 104 metallopeptidase activity ‐1.26333 0.000178 3 zinc ion binding

hydrolase activity

dendrite collagen catabolic process

extracellular matrix

protein complex binding

protein complex

cell body

metal ion binding Leukocyte transendoth skeletal system development elial migration

Pathways in skeletal system development cancer

Bladder fibronectin binding cancer

metalloendopeptidase activity

metalloendopeptidase activity

metalloendopeptidase activity

extracellular region

proteinaceous extracellular matrix

extracellular space

proteolysis

proteolysis

response to oxidative stress

metabolic process

peptidase activity

peptidase activity

matrix metallopeptidase activity 2 96.0 cM 2 580338 Mmp9 metallopeptidase 2 164773750 NM_013599 17395 Mm.4406 522 0.92 0.005341 H1‐H2 9 metallopeptidase activity

zinc ion binding

hydrolase activity transformation of host cell by virus

extracellular matrix organization

extracellular matrix organization

collagen catabolic process

collagen catabolic process

extracellular matrix

positive regulation of synaptic plasticity

protein complex binding

response to drug

positive regulation of apoptosis

protein complex

positive regulation of angiogenesis

metal ion binding

tissue remodeling

cellular response to cell‐matrix adhesion cell adhesion myelin protein 9 26.0 cM 9 5690538 Mpzl2 9 44850426 NM_007962 14012 Mm.33240 16 membrane ‐0.513333 0.003512 zero‐like 2 A5.2 integral to membrane molecular_function

cellular_component

mitochondrion mitochondrial 2260075 Mrpl55 ribosomal protein 11 59016018 NM_026035 67212 11 11 B2 Mm.282694 12 mitochondrion ‐0.713333 0.000966 L55 ribosome

biological_process

ribonucleoprotein complex molecular_function MAP7 domain 130471 Mtap7d2 X 155852509 NM_001081124 78283 X X F4 Mm.160079 10 cellular_component ‐0.806667 0.000391 containing 2 biological_process molecular_function nucleus

musculoskeletal, nucleus 6380309 Mustn1 embryonic 14 31692442 NM_181390 66175 14 14 B Mm.220895 14 ‐0.686667 0.008465 nuclear protein 1 nucleoplasm

wound healing

tissue regeneration nucleotide binding

molecular_function

motor activity

actin binding

2970358 Myo1g myosin IG 11 ‐6406551 NM_178440 246177 11 11 A1 Mm.239554 11 calmodulin binding 1.26 0.000245

ATP binding

cellular_component

biological_process

myosin complex Glycosamin oglycan MAPKKK cascade biosynthesis ‐ heparan sulfate

Metabolic polysaccharide biosynthetic process pathways

catalytic activity

Golgi apparatus

protein amino acid deacetylation

protein amino acid sulfation

protein amino acid sulfation

inflammatory response

smoothened signaling pathway

respiratory gaseous exchange

sulfotransferase activity fibroblast growth factor receptor signaling N‐deacetylase/N‐ pathway sulfotransferase 5340121 Ndst1 18 ‐60845629 NM_008306 15531 18 18 D2 Mm.181862 48 0.84 0.003659 (heparan embryo development glucosaminyl) 1 organ morphogenesis

[heparan sulfate]‐glucosamine N‐ sulfotransferase activity

[heparan sulfate]‐glucosamine N‐ sulfotransferase activity

membrane

integral to membrane

transferase activity

hydrolase activity

glycosaminoglycan metabolic process

forebrain development

forebrain development

midbrain development

embryonic neurocranium morphogenesis

embryonic viscerocranium morphogenesis Oxidative molecular_function phosphoryla tion

Metabolic mitochondrion pathways

Alzheimer's mitochondrion disease

NADH Parkinson's mitochondrial inner membrane dehydrogenase disease 5900576 Ndufb10 (ubiquinone) 1 17 ‐24859011 NM_026684 68342 17 17 A3.3 Mm.1129 14 ‐3.15 1.30E‐05 beta subcomplex, Huntington' mitochondrial inner membrane 10 s disease

transport

biological_process membrane

electron transport chain

respiratory chain molecular_function

plasma membrane

transport

endocytosis NECAP 6200048 Necap2 endocytosis 4 ‐140622427 NM_025383 66147 4 4 D3 Mm.21187 17 biological_process 1.44667 0.00146 associated 2 protein transport

membrane

clathrin vesicle coat

cytoplasmic vesicle extracellular region

extracellular space NM_025424, neuron derived growth factor activity XR_032019 4210731 Nenf neurotrophic 0.956667 5.30E‐05 NM_025424, factor heme binding XR_032019 positive regulation of MAPKKK cascade

metal ion binding molecular_function

cellular_component

transport NIPA‐like domain 6130315 Nipal1 5 73039034 NM_001081205 70701 5 5 C3.2 Mm.38884 9 ion transport ‐1.06 0.00111 containing 1 magnesium ion transport

membrane

integral to membrane molecular_function

cellular_component

plasma membrane natural killer cell 1780390 Nkg7 7 50692507 NM_024253 72310 7 7 B2 Mm.34613 10 WT Only WT Only group 7 sequence biological_process membrane

integral to membrane molecular_function

cellular_component

plasma membrane natural killer cell 6370059 Nkg7 7 50692507 NM_024253 72310 7 7 B2 Mm.34613 10 WT Only WT Only group 7 sequence biological_process

membrane

integral to membrane gastric acid secretion

extracellular region

regulation of smooth muscle contraction

response to stress

neuropeptide signaling pathway

neuropeptide signaling pathway NM_019515, sensory perception of pain XM_001475124 6400025 Nmu neuromedin U WT Only WT Only NM_019515, eating behavior XM_001475124 neuromedin U receptor binding

terminal button

positive regulation of smooth muscle contraction

positive regulation of hormone secretion

positive regulation of synaptic transmission G‐protein‐coupled receptor binding

G‐protein‐coupled receptor binding

hormone activity

extracellular region

extracellular space neuropeptide signaling pathway Adipocytoki 6 26.0 cM 6 3170138 Npy neuropeptide Y 6 49772727 NM_023456 109648 Mm.154796 235 neuropeptide signaling pathway ne signaling ‐0.703333 0.004428 B3 pathway synaptic transmission

regulation of blood pressure

adult feeding behavior

neuropeptide Y receptor binding

positive regulation of appetite

negative regulation of blood pressure DNA binding

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

steroid hormone receptor activity

receptor activity

ligand‐dependent nuclear receptor activity

nuclear receptor thyroid hormone receptor activity subfamily 1, 2360014 Nr1d2 14 ‐19036569 NM_011584 353187 14 14 A2 Mm.26587 22 ‐0.973333 0.005811 group D, member cellular_component 2 nucleus

transcription

regulation of transcription, DNA‐dependent

regulation of transcription, DNA‐dependent

zinc ion binding

sequence‐specific DNA binding

regulation of transcription

metal ion binding plasma membrane nervous system development

4230471 Nrn1 neuritin 113‐36817494 NM_153529 68404 13 13 A3.3 Mm.232930 19 axonogenesis ‐0.85 0.001772

membrane

anchored to membrane nucleotide binding

ATP‐dependent peptidase activity

serine‐type endopeptidase activity

binding

protein binding

ATP binding

cytoplasm

ATP catabolic process

proteolysis

transport Vasopressin‐ N‐ethylmaleimide potassium ion transport regulated 11 63.0 cM 11 4070075 Nsf sensitive fusion 11 ‐103683096 NM_008740 18195 Mm.260117 39 water 0.673333 0.002583 E1 protein protein C‐terminus binding reabsorptio n protein transport

protein transport

vesicle‐mediated transport

hydrolase activity

nucleoside‐triphosphatase activity

regulation of exocytosis

syntaxin binding

ATPase activity, coupled

dendritic shaft

metal ion binding acute inflammatory response

molecular_function cellular_component

nucleus

response to toxin 1990524 Nupr1 nuclear protein 17 ‐133766759 NM_019738 56312 7 7 F4 Mm.18742 30 ‐1.03667 0.001012 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis

positive regulation of apoptosis

negative regulation of fibroblast proliferation negative regulation of transcription from RNA polymerase II promoter

molecular_function

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

steroid hormone receptor activity

receptor activity

ligand‐dependent nuclear receptor activity

cellular_component

nucleus occludin/ELL 2470088 Ocel1 domain NM_029865 1.14 0.000453 transcription containing 1

regulation of transcription, DNA‐dependent

biological_process

zinc ion binding

entrainment of circadian clock by photoperiod

sequence‐specific DNA binding

sequence‐specific DNA binding regulation of transcription

metal ion binding

neuron development

detection of temperature stimulus involved in sensory perception of pain nucleotide binding

protein kinase activity

protein serine/threonine kinase activity

protein binding

ATP binding oxidative‐stress 1500025 Oxsr1 9 ‐119149783 108737 9 9 F3 Mm.293565 24 1.02333 0.001927 responsive 1 protein amino acid phosphorylation

response to oxidative stress

kinase activity

transferase activity

metal ion binding

PEST proteolytic 1.07E+08 Pcnp signal containing NM_001024622* ‐0.87 0.004928 nuclear protein molecular_function

platelet‐derived cellular_component 870168 Pdgfrl growth factor 8 42011586 NM_026840 68797 8 8 A4 Mm.284246 10 1.91 0.000336 receptor‐like extracellular region

biological_process cell morphogenesis

ruffle

lymphangiogenesis

water transmembrane transporter activity

plasma membrane

plasma membrane

plasma membrane prostaglandin metabolic process

water transport

amino acid transport

cellular component movement

cell cycle

cell adhesion

signal transduction

multicellular organismal development

cell proliferation

regulation of cell shape

4640280 Pdpn podoplanin 4 ‐142857324 NM_010329 14726 4 4 E1 Mm.2976 96folic acid transporter activity 1.63 8.90E‐05

positive regulation of platelet activation

amino acid transmembrane transporter activity

water channel activity

folic acid transport

membrane

integral to membrane

apical plasma membrane

cell‐cell adhesion

lamellipodium

filopodium

lung development

positive regulation of cell migration

tube morphogenesis

cell projection

lung alveolus development positive regulation of cellular component movement molecular_function

cellular_component PDZK1 interacting 4850364 Pdzk1ip1 4 114761312 NM_026018 67182 4 4 D1 Mm.30181 18 biological_process ‐0.883333 0.002307 protein 1 membrane

integral to membrane molecular_function pore forming 6450670 Pfpl 19 12502394 NM_019540 56093 19 19 A Mm.285849 11 cellular_component 1.13 0.002184 protein‐like biological_process receptor activity

extracellular region Intestinal polymeric plasma membrane immune 1 68.2 cM 1 6520441 Pigr immunoglobulin 1 132723260 NM_011082 18703 Mm.276414 38 network for ‐0.646667 0.002012 E3 receptor membrane IgA production integral to membrane

transport vesicle nucleotide binding

protein kinase activity

protein kinase activity

protein kinase activity

protein serine/threonine kinase activity

protein kinase C activity

signal transducer activity

receptor activity

G‐protein coupled receptor activity

prostaglandin receptor activity

prostaglandin E receptor activity

prostaglandin E receptor activity

ATP binding

intracellular nucleus

cytoplasm

cytoplasm 2760647 Pkn1 protein kinase N1 NM_177262 0.99 0.002012 endosome

plasma membrane

transcription

protein amino acid phosphorylation

protein amino acid phosphorylation

hyperosmotic response

signal transduction

G‐protein coupled receptor protein signaling pathway

membrane

integral to membrane

cytoplasmic membrane‐bounded vesicle

kinase activity

phosphorylation

chromatin modification

transferase activity

regulation of transcription Inositol follicular B cell differentiation phosphate metabolism

Metabolic phosphoinositide phospholipase C activity pathways

ErbB phosphoinositide phospholipase C activity signaling pathway Calcium phospholipase C activity signaling pathway

Phosphatidy linositol phospholipase C activity signaling system

VEGF signal transducer activity signaling pathway

Natural killer cell protein binding mediated cytotoxicity

B cell receptor protein binding signaling pathway

Fc epsilon RI membrane fraction signaling pathway

Fc gamma R‐ mediated lipid metabolic process phagocytosi phospholipase C, 8 62.0 cM 8 5720008 Plcg2 8 120022190 NM_172285 234779 Mm.192699 88s 0.936667 0.002882 gamma 2 E1

Leukocyte transendoth signal transduction elial migration

Neurotrophi elevation of cytosolic calcium ion n signaling concentration pathway

Pathways in phosphoric diester hydrolase activity cancer

metabolic process Glioma

Non‐small phospholipid catabolic process cell lung cancer regulation of gene expression

lipid catabolic process

hydrolase activity

intracellular signaling pathway

response to magnesium ion

activation of store‐operated calcium channel activity

response to lipopolysaccharide

inositol trisphosphate biosynthetic process

response to ATP

negative regulation of programmed cell death

T cell receptor signaling pathway iron ion binding

endoplasmic reticulum

procollagen‐lysine 5‐dioxygenase activity

membrane

oxidoreductase activity procollagen oxidoreductase activity, acting on single lysine, 2‐ 9 52.0 cM 9 Lysine 3360427 Plod2 9 92437060 NM_011961 26432 Mm.79983 25 donors with incorporation of molecular ‐0.643333 0.004164 oxoglutarate 5‐ E3.3 degradation oxygen, incorporation of two atoms of dioxygenase 2 oxygen

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

L‐ascorbic acid binding

metal ion binding

oxidation reduction

Glycerolipid molecular_function metabolism Metabolic triglyceride lipase activity pathways

lipid metabolic process

biological_process patatin‐like metabolic process phospholipase 5050551 Pnpla3 15 83998245 NM_054088 116939 15 15 E3 Mm.390817 14 1.17667 0.002484 domain acyltransferase activity containing 3 membrane

membrane

integral to membrane

lipid catabolic process

transferase activity

hydrolase activity ribonuclease MRP complex

molecular_function

RNA binding

ribonuclease P activity

ribonuclease activity

cellular_component processing of precursor 4, nucleus 4560600 Pop4 ribonuclease 7 ‐39047838 NM_025390 66161 7 7 B1 Mm.22284 13 0.793333 0.003681 P/MRP family, (S. nucleolar ribonuclease P complex cerevisiae) rRNA processing

mRNA cleavage

tRNA processing

biological_process

hydrolase activity

ribonuclease P complex molecular_function

carboxypeptidase activity

cellular_component lysosome prolylcarboxypept 7050746 Prcp idase 7 100023762 NM_028243 72461 7 7 E2 Mm.389969 14 proteolysis ‐1.48667 0.000797 (angiotensinase C) biological_process

peptidase activity

serine‐type peptidase activity

hydrolase activity activation of MAPK activity

respiratory burst involved in inflammatory response

peroxidase activity

peroxidase activity

protein binding

cytoplasm

mitochondrion

cytosol

oxygen and reactive oxygen species metabolic process

anti‐apoptosis

anti‐apoptosis

response to oxidative stress

response to oxidative stress

metabolic process

thioredoxin peroxidase activity

thioredoxin peroxidase activity

selenium binding

regulation of hydrogen peroxide metabolic 8 36.0 cM 8 Mm.347009 process 4010619 Prdx2 peroxiredoxin 2 8 87493546 21672 47 ‐1.96 0.00023 C3 Mm.393373 negative regulation of oxygen and reactive oxygen species metabolic process antioxidant activity

oxidoreductase activity negative regulation of lipopolysaccharide‐ mediated signaling pathway

negative regulation of NF‐kappaB transcription factor activity

response to lipopolysaccharide

T cell proliferation

hydrogen peroxide metabolic process

hydrogen peroxide catabolic process

negative regulation of neuron apoptosis

cell redox homeostasis negative regulation of T cell differentiation

thymus development

homeostasis of number of cells

peroxiredoxin activity

oxidation reduction activation of MAPK activity respiratory burst involved in inflammatory response

peroxidase activity

peroxidase activity

protein binding

cytoplasm

mitochondrion

cytosol

oxygen and reactive oxygen species metabolic process anti‐apoptosis

anti‐apoptosis

response to oxidative stress

response to oxidative stress

metabolic process

thioredoxin peroxidase activity

thioredoxin peroxidase activity

selenium binding

regulation of hydrogen peroxide metabolic 8 36.0 cM 8 Mm.347009 process 5340577 Prdx2 peroxiredoxin 2 8 87493546 21672 47 KO Only KO Only C3 Mm.393373 negative regulation of oxygen and reactive oxygen species metabolic process

antioxidant activity

oxidoreductase activity

negative regulation of lipopolysaccharide‐ mediated signaling pathway

negative regulation of NF‐kappaB transcription factor activity

response to lipopolysaccharide

T cell proliferation

hydrogen peroxide metabolic process

hydrogen peroxide catabolic process

negative regulation of neuron apoptosis

cell redox homeostasis

negative regulation of T cell differentiation

thymus development

homeostasis of number of cells

peroxiredoxin activity oxidation reduction Natural killer cell extracellular region mediated cytotoxicity

Type I plasma membrane diabetes mellitus

Autoimmun circadian rhythm e thyroid disease perforin 1 (pore 10 36.0 cM 10 6660309 Prf1 10 60760583 NM_011073 18646 Mm.240313 306Allograft WT Only WT Only forming protein) B4 membrane rejection

Graft‐versus‐ integral to membrane host disease

Viral cytoplasmic membrane‐bounded vesicle myocarditis

cytolysis

cytolysis response to hypoxia

prolactin family 4, prolactin receptor binding 13 14.0 cM 13 6980427 Prl4a1 subfamily a, 13 28108091 NM_011165 19110 Mm.18607 19 0.973333 0.00592 A3.1 member 1 hormone activity

extracellular region catalytic activity

serine‐type endopeptidase activity

extracellular region

extracellular region

proteolysis protease, serine, 17 10.43 cM 4670427 Prss28 17 25445590 NM_053259 114661 Mm.207081 15 embryo implantation ‐0.886667 8.50E‐05 28 17 A3.3 metabolic process

peptidase activity serine‐type peptidase activity

serine‐type peptidase activity

hydrolase activity molecular_function

catalytic activity

serine‐type endopeptidase activity protease, serine, 3450725 Prss35 9 86636433 244954 9 9 E3.1 Mm.257629 15 extracellular region 0.756667 0.008848 35 mitochondrion

proteolysis

biological_process cytokinesis

stress fiber

actin binding

catalytic activity

proline‐serine‐ protein binding NOD‐like threonine receptor 3190156 Pstpip1 phosphatase‐ 9 55937782 NM_011193 19200 9 9 C Mm.2534 18 cytoplasm 1.54 0.000213 signaling interacting pathway protein 1 actomyosin contractile ring

cytoskeleton

cell adhesion

protein phosphatase binding

cell projection regulation of cell growth Cell cycle

cysteine‐type endopeptidase inhibitor Oocyte activity meiosis

protein binding

nucleus

cytoplasm

DNA metabolic process

DNA repair response to DNA damage stimulus

cell cycle

pituitary tumor‐ chromosome segregation ‐43233751, ‐ 520463 Pttg1 transforming gene 11 NM_013917 30939 11 11 A5 Mm.6856 54 ‐0.643333 0.002056 43233751 1 mitosis

negative regulation of cell proliferation

cellular process

negative regulation of endopeptidase activity

SH3 domain binding

heat shock protein binding

ribosome binding

homologous chromosome segregation

chromosome organization

cell division RAB44, member 1.02E+08 Rab44 RAS oncogene 1.16 0.000588 family Nucleotide damaged DNA binding excision repair

Protein processing nucleus in endoplasmi c reticulum RAD23a homolog 670082 Rad23a 8 ‐87358550 NM_009010 19358 8 8 C3 Mm.255539 16 ‐1.03667 0.002609 (S. cerevisiae) DNA repair

nucleotide‐excision repair

response to DNA damage stimulus

response to DNA damage stimulus

proteasomal ubiquitin‐dependent protein catabolic process Nucleotide damaged DNA binding excision repair Protein processing nucleus in endoplasmi c reticulum RAD23a homolog 5360463 Rad23a 8 ‐87358550 NM_009010 19358 8 8 C3 Mm.255539 16 ‐0.626667 0.00105 (S. cerevisiae) DNA repair

nucleotide‐excision repair

response to DNA damage stimulus

response to DNA damage stimulus

proteasomal ubiquitin‐dependent protein catabolic process Nucleotide damaged DNA binding excision repair

Protein processing nucleus in endoplasmi c reticulum RAD23a homolog 5550364 Rad23a 8 ‐87358550 NM_009010 19358 8 8 C3 Mm.255539 16 ‐0.933333 0.001532 (S. cerevisiae) DNA repair

nucleotide‐excision repair

response to DNA damage stimulus

response to DNA damage stimulus

proteasomal ubiquitin‐dependent protein catabolic process molecular_function Rap1 GTPase‐ 4 66.3 cM 4 1.02E+08 Rap1gap 4 137237617 NM_001081155 110351 Mm.180763 35 cellular_component 1.12667 0.00111 activating protein D3 biological_process nucleotide binding

GTPase activity

GTP binding

GTP binding

cellular_component

intracellular plasma membrane

signal transduction

RASD family, 3360010 Rasd2 8 77737842 XM_204287 75141 8 8 C1 Mm.179267 17 small GTPase mediated signal transduction 0.626667 0.000966 member 2

behavior

locomotory behavior

membrane

negative regulation of protein ubiquitination

ubiquitin conjugating enzyme binding

positive regulation of protein sumoylation

phosphoinositide 3‐kinase binding molecular_function

guanyl‐nucleotide exchange factor activity

cellular_component RasGEF domain 11 11 B1.2‐ intracellular 1.05E+08 Rasgef1c family, member 11 49715336 NM_029004 74563 Mm.258798 7 WT Only WT Only B1.3 1C small GTPase mediated signal transduction

biological_process

regulation of small GTPase mediated signal transduction hormone activity 16 32.6 cM 16 3840138 Retnla resistin like alpha 16 48842664 NM_020509 57262 Mm.441868 34 extracellular region 1.11333 0.003817 B5 extracellular region nucleus

cytoplasm

DNA‐dependent DNA replication

DNA repair

DNA recombination RPA interacting 1.03E+08 Rpain 11 70783714 NM_027186 69723 11 11 B4 Mm.275691 12 0.933333 0.000817 protein protein protein import into nucleus

response to UV

PML body

protein complex binding

metal ion binding nucleotide binding

structural constituent of ribosome

structural constituent of ribosome NM_011297, NM_207634, intracellular NM_207635 NM_011297, ribosome ribosomal protein 5420377 Rps24 14 25309902 NM_207634, 20088 14 14 B Mm.16775 17 Ribosome 0.693333 0.006104 S24 NM_207635 translation NM_011297, NM_207634, translation NM_207635 cytosolic small ribosomal subunit

ribonucleoprotein complex

translation initiation factor binding molecular_function

cellular_component ribosomal protein 460092 Rps4y2 6 148303177 NR_003634 66184 6 6 G3 Mm.391542 10 0.983333 0.003512 S4, Y‐linked 2 ribosome

biological_process pseudouridine synthesis

molecular_function

RNA binding RNA pseudouridylate 2450725 Rpusd4 9 35075465 NM_028040 71989 9 9 A5.3 Mm.273515 12 mitochondrion ‐0.873333 0.001773 synthase domain containing 4 biological_process

RNA modification

pseudouridine synthase activity

radial spoke head cytoplasm 17 13.25 cM 6900717 Rsph1 1 homolog 17 ‐31391964 NM_025290 22092 Mm.12743 20 0.76 0.004929 17 A3.3 (Chlamydomonas) meiosis molecular_function NM_001037709, RUN and SH3 cellular_component 43394853, NM_199057 460168 Rusc2 domain 4 100213 4 4 A5 Mm.235730 13 ‐1.64333 0.005811 43419445 NM_001037709, containing 2 cytoplasm NM_199057 biological_process negative regulation of transcription from RNA polymerase II promoter

calcium ion binding

protein binding S100 calcium 3 43.6 cM 3 6770707 S100a1 binding protein 3 ‐90314955 NM_011309 20193 Mm.24662 44 ‐0.6 0.004928 F1‐F2 cytoplasm A1 sarcoplasmic reticulum

identical protein binding

metal ion binding calcium ion binding

extracellular region

extracellular space

S100 calcium cytoplasm 3 43.6 cM 3 70112 S100a8 binding protein 3 90472992 NM_013650 20201 Mm.21567 54 ‐1.01333 0.002855 F1‐F2 A8 (calgranulin A) cytoskeleton

plasma membrane

chemotaxis

membrane Steroid C‐4 methylsterol oxidase activity biosynthesis

Metabolic molecular_function pathways

iron ion binding

cellular_component

endoplasmic reticulum

fatty acid biosynthetic process sterol‐C4‐methyl 3140524 Sc4mol 8 ‐67196941 NM_025436 66234 8 8 B3.1 Mm.30119 13 ‐0.59 0.002484 oxidase‐like steroid biosynthetic process

biological_process lipid biosynthetic process

membrane

integral to membrane

sterol biosynthetic process

oxidoreductase activity

oxidation reduction molecular_function

cytokine activity NM_054037, secretoglobin, cellular_component NM_170727 11 25.0 cM 11 5900333 Scgb3a1 family 3A, 11 49477111 68662 Mm.22802 21 ‐0.986667 0.009117 NM_054037, B1.2 member 1 extracellular region NM_170727 extracellular space

biological_process molecular_function

cellular_component NM_001039137, short coiled‐coil ‐85958391, ‐ NM_019708 2230053 Scoc 8 56367 8 8 C2 Mm.246911 13 nucleus ‐1.97667 1.20E‐05 protein 85958391 NM_001039137, NM_019708 Golgi apparatus

biological_process 6370411 Sdc4 syndecan 4 ‐0.56 0.002321 molecular_function

syndecan binding protein binding 3840037 Sdcbp2 protein (syntenin) 2 151398367 NM_145535 228765 2 2 G3 Mm.32068 11 ‐0.853333 0.005289 2 cellular_component

biological_process molecular_function

syndecan binding protein binding 1400014 Sdcbp2 protein (syntenin) 2 151398367 NM_145535 228765 2 2 G3 Mm.32068 11 ‐0.706667 0.008166 2 cellular_component

biological_process protein binding

sugar binding

plasma membrane Cell selectin, platelet adhesion 1770167 Selplg 5 ‐114267806 NM_009151 20345 5 64.0 cM 5 F Mm.332590 103 cell adhesion 1.40667 0.002032 (p‐selectin) ligand molecules (p‐selectin) ligand molecules (CAMs) membrane

integral to membrane

leukocyte tethering or rolling receptor activity sema domain, multicellular organismal development immunoglobulin domain (Ig), ‐88239883, ‐ nervous system development transmembrane 88239883, ‐ Axon 5890022 Sema4a 3 NM_013658 20351 3 3 F1 Mm.439752 24 0.496667 0.00023 domain (TM) and 88239883, ‐ guidance membrane short cytoplasmic 88239883 domain, integral to membrane (semaphorin) 4A cell differentiation cytoplasm

mitochondrion

peroxisome

NM_023475, peroxisome serine hydrolase‐ NM_029101* 2470605 Serhl 15 82930634 68607 15 15 E1 Mm.30056 12 1.08333 0.001336 like NM_023475, metabolic process NM_029101* cytoplasmic membrane‐bounded vesicle

hydrolase activity

hydrolase activity serine (or molecular_function cysteine) 13 13.6 cM 13 730047 Serpinb6c peptidase 13 ‐33971684 NM_148942 97848 Mm.272188 7 cellular_component Amoebiasis ‐0.973333 0.00111 A3.3 inhibitor, clade B, member 6c biological_process SH3/SH2 adaptor activity

protein binding

protein binding

cytoplasm NM_001025571, VEGF SH2 domain NM_021309 3 42.6 cM 3 2970594 Sh2d2a 3 87650676 27371 Mm.86361 21 plasma membrane signaling WT Only WT Only protein 2A NM_001025571, F1 pathway NM_021309 signal transduction

cell proliferation

membrane SH3 domain binding nucleoside transmembrane transporter activity

nucleoside transmembrane transporter activity

nucleoside transmembrane transporter activity

plasma membrane

integral to plasma membrane

solute carrier transport family 29 2970379 Slc29a1 (nucleoside 17 ‐45722170 NM_022880 63959 17 17 C Mm.29744 29 nucleoside transport 0.603333 0.001772 transporters), member 1 nucleoside transport

uridine transport

membrane

integral to membrane

sleep

transmembrane transport

regulation of excitatory postsynaptic membrane potential zinc ion transmembrane transporter activity

plasma membrane

plasma membrane

transport

ion transport

iron ion transport

zinc ion transport

zinc ion transport solute carrier family 39 (zinc ‐70703273, ‐ ferrous iron transmembrane transporter 670300 Slc39a14 14 213053 14 14 D2 Mm.270647 20 ‐0.94 0.004127 transporter), 70703273 activity member 14 ferrous iron transport membrane

integral to membrane

metal ion transport

iron ion transmembrane transport

metal ion transmembrane transporter activity

transmembrane transport

zinc ion transmembrane transport transporter activity

integral to plasma membrane

transport solute carrier prostaglandin transmembrane transporter organic anion 9 51.0 cM 9 Mm.207106 activity 1.01E+08 Slco2a1 transporter 9 102910818 NM_033314 24059 15 0.76 0.002109 F1 Mm.412758 family, member prostaglandin transport 2a1 membrane

integral to membrane

transmembrane transport serine‐type endopeptidase inhibitor activity secretory leukocyte extracellular region 2120446 Slpi 2 ‐164179806 NM_011414 20568 2 2 H Mm.371583 31 KO Only KO Only peptidase inhibitor peptidase activity

peptidase inhibitor activity molecular_function

cytoplasm

cytoplasm

cytoskeleton

adult locomotory behavior

14 12.5 cM 14 regulation of dopamine secretion 3120725 Sncg synuclein, gamma 14 ‐35183459 NM_011430 20618 Mm.282800 42 ‐0.513333 0.00035 B‐C axon

neuronal cell body neuronal cell body

axon terminus

regulation of neurotransmitter secretion actin binding

protein binding

protein binding

calmodulin binding

cytoplasm

cytoskeleton syntrophin, acidic 2 84.0 cM 2 4570400 Snta1 2 ‐154202049 NM_009228 20648 Mm.1541 62 plasma membrane ‐0.686667 0.000588 1 H1 neuromuscular junction development

membrane

cell junction

PDZ domain binding

sarcolemma

postsynaptic membrane molecular_function

protein binding

cellular_component

transport 51473901, 3800035 Snx10 sorting nexin 10 6 NM_028035 71982 6 6 B3 Mm.480375 12 0.496667 0.002074 51494521 cell communication

biological_process

protein transport

phosphoinositide binding cartilage condensation

cell fate specification

epithelial to mesenchymal transition

hair follicle development DNA binding

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

protein binding

nucleus

nucleus

transcription

regulation of transcription, DNA‐dependent

spermatogenesis

heart development

male gonad development

male gonad development

positive regulation of gene expression

promoter binding

neural crest cell development

negative regulation of transcription

transcription activator activity

male germ‐line sex determination SRY‐box 11 69.5 cM 11 Mm.286407 5720681 Sox9 11 112643523 NM_011448 20682 491 ‐0.55 0.001363 containing gene 9 E2 Mm.481669 cell differentiation

male sex determination

negative regulation of bone mineralization

negative regulation of chondrocyte differentiation

regulation of cell proliferation

regulation of apoptosis sequence‐specific DNA binding

cell fate commitment

regulation of transcription

negative regulation of myoblast differentiation

negative regulation of transcription, DNA‐ dependent

positive regulation of transcription, DNA‐ dependent

positive regulation of transcription from RNA polymerase II promoter

positive regulation of transcription from RNA polymerase II promoter

oligodendrocyte differentiation

cartilage development

Sertoli cell differentiation

Sertoli cell development

endochondral bone morphogenesis

prostate gland morphogenesis

epithelial cell proliferation involved in prostatic bud elongation sphingosine 1.07E+08 Sphk1 0.89 0.000561 kinase 1 molecular_function

serine‐type endopeptidase inhibitor activity

serine peptidase cellular_component 3990093 Spink2 inhibitor, Kazal 5 ‐77634132 NM_183284 69982 5 5 C3.3 Mm.46106 9 1.23667 0.002056 type 2 extracellular region

biological_process

peptidase inhibitor activity serine‐type endopeptidase inhibitor activity

serine peptidase extracellular region 1580008 Spink3 inhibitor Kazal 18 ‐43887722 NM 009258 20730 18 18 B3 Mm 272 25 ‐0 823333 0 001882 1580008 Spink3 inhibitor, Kazal 18 ‐43887722 NM_009258 20730 18 18 B3 Mm.272 25 ‐0.823333 0.001882 type 3 peptidase inhibitor activity

negative regulation of proteolysis Focal ossification adhesion

ECM‐ osteoblast differentiation receptor interaction

Toll‐like receptor cytokine activity signaling pathway

cytokine activity

extracellular region

extracellular region

extracellular space

extracellular space

cytoplasm

secreted 5 56.0 cM 5 anti‐apoptosis 2470609 Spp1 5 104864136 NM_009263 20750 Mm.288474 429 3.58 2.00E‐06 phosphoprotein 1 E5 cell adhesion

cell adhesion

response to organic substance

positive regulation of cell‐substrate adhesion

biomineral tissue development

membrane‐bounded vesicle

cell projection

apical part of cell

positive regulation of bone resorption

perinuclear region of cytoplasm

response to steroid hormone stimulus

extracellular matrix binding cornified envelope

cytoplasm

epidermis development small proline‐rich 3 45.2 cM 3 1340458 Sprr2d 3 92143061 NM_011470 20758 Mm.87820 17 1.36667 0.000417 protein 2D F1 keratinocyte differentiation

keratinization

response to estradiol stimulus GTPase activator activity

protein binding

SLIT‐ROBO Rho cellular_component Axon 6130215 Srgap3 GTPase activating 6 ‐112667965 NM_080448 259302 6 6 E3 Mm.236401 21 0.593333 0.000638 guidance protein 3 intracellular

signal transduction

biological_process calcium ion binding

protein binding

intracellular

cytoplasm

plasma membrane 8046079, 5 1.0 cM 5 A1‐ 1.01E+08 Sri sorcin 5 109552 Mm.96211 22 1.99333 0.006488 8056541 h calcium ion transport

membrane

sarcoplasmic reticulum

Z disc

vesicle calcium ion binding

protein binding

intracellular

cytoplasm NM_001080974, plasma membrane 8046079, NM_025618 5 1.0 cM 5 A1‐ 4850064 Sri sorcin 5 109552 Mm.96211 22 ‐0.65 0.003092 8056541 NM_001080974, h calcium ion transport NM_025618 membrane sarcoplasmic reticulum

Z disc

vesicle nucleotide binding

DNA binding

ATP binding

cytoplasm

cytosol

response to oxidative stress sulfiredoxin 1 3130195 Srxn1 homolog (S. 2 151931465 NM_029688 76650 2 2 H1 Mm.218639 18 ‐0.883333 0.002546 metabolic process cerevisiae) antioxidant activity

oxidoreductase activity

oxidoreductase activity, acting on sulfur group of donors

sulfiredoxin activity

oxidation reduction plasma membrane

membrane

integral to membrane 6 71.55 cM 6 4670091 Sspn sarcospan 6 145882666 NM_010656 16651 Mm.49689 23 cell junction KO Only KO Only G3 sarcolemma

synapse

postsynaptic membrane Glycosphing olipid biosynthesis Golgi apparatus ‐ lacto and neolacto series

Metabolic protein amino acid glycosylation pathways ST3 beta‐ galactoside alpha‐ galactoside alpha‐ 6350020 St3gal4 9 ‐34854163 NM_009178 20443 9 9 A5.3 Mm.275973 21 sialyltransferase activity ‐0.71 0.002202 2,3‐ sialyltransferase 4 membrane

integral to membrane

transferase activity

transferase activity, transferring glycosyl groups

integral to Golgi membrane XM_001472888, XM_001473721, XM_203312, XM_896683, XM_896707, XM_920202, XM_920212, XM_920225 XM_001472888, XM_001473721, XM_203312, XM_896683, XM_896707, XM_920202, SNAP receptor activity XM_920212, XM_920225 XM_001472888, XM_001473721, XM_203312, XM_896683, SNARE XM_896707, interactions 6590184 Stx11 syntaxin 11 10 ‐12659786 74732 10 10 A1 Mm.248648 9 0.636667 0.001474 XM_920202, in vesicular XM_920212, transport XM_920225 XM_001472888, XM_001473721, XM_203312, XM_896683, XM_896707, XM_920202, protein binding XM_920212, XM_920225 Golgi apparatus XM_001472888, XM_001473721, transport XM_203312, XM_896683, intracellular protein transport XM_896707, XM_920202, protein transport XM_920212, XM_920225 membrane XM_001472888, XM 001473721, vesicle‐mediated transport molecular_function GTPase activator activity

Rab GTPase activator activity

cellular_component TBC1 domain 7000286 Tbc1d20 family, member 2 152119607 NM_024196 67231 2 2 H1 Mm.288733 13 intracellular 1.28667 0.000203 20 biological_process

membrane

integral to membrane

regulation of Rab GTPase activity DNA binding

DNA binding

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

protein binding

transcription nucleus 172375092, 840025 Tcfap2c factor AP‐2, 2 NM_009335 21420 2 2 H3‐H4 Mm.3629 52 0.646667 0.001461 172376490 gamma nucleus

transcription

regulation of transcription, DNA‐dependent

regulation of transcription, DNA‐dependent

regulation of transcription from RNA polymerase II promoter

cell differentiation

regulation of transcription DNA binding

DNA binding sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

sequence‐specific DNA binding transcription factor activity

protein binding

transcription nucleus 172375092, 4670039 Tcfap2c factor AP‐2, 2 NM_009335 21420 2 2 H3‐H4 Mm.3629 52 0.576667 0.003394 172376490 gamma nucleus

transcription

regulation of transcription, DNA‐dependent

regulation of transcription, DNA‐dependent

regulation of transcription from RNA polymerase II promoter

cell differentiation

regulation of transcription

T‐cell receptor 6 20.5 cM 6 Mm.123831 3610048 Tcrb‐V8.2 6 21607 13 WT Only WT Only beta, variable 8.2 B1 Mm.333026 molecular_function

thioesterase mitochondrion 1.05E+08 Them5 superfamily 3 94146020 66198 3 3 F2 Mm.180200 9 ‐0.653333 0.00348 member 5 biological_process

hydrolase activity molecular_function

metalloendopeptidase activity

cellular_component

extracellular region NM_001040426, thrombospondin, ‐59814738, ‐ NM_172444 Mm.28791 6040037 Thsd4 type I, domain 9 207596 9 9 B 13 proteinaceous extracellular matrix 0.966667 0.002279 59814738 NM_001040426, Mm.379523 containing 4 NM_172444 biological_process

peptidase activity

hydrolase activity extracellular matrix angiogenesis

Rho GTPase activator activity

cytosol

plasma membrane

plasma membrane

negative regulation of protein kinase activity

cytoskeleton organization

external side of plasma membrane

cell surface

membrane

cell‐cell adhesion

enzyme binding

protein kinase binding

negative regulation of cell migration Leukocyte dendrite thymus cell 9 26.0 cM 9 transendoth 5910162 Thy1 9 43851466 NM_009382 21838 Mm.3951 158 0.653333 0.001462 antigen 1, theta A5.1 elial growth cone migration anchored to membrane

anchored to external side of plasma membrane

GPI anchor binding

positive regulation of GTPase activity

membrane raft

retinal cone cell development

anchored to plasma membrane

focal adhesion assembly

negative regulation of axonogenesis T cell receptor signaling pathway

negative regulation of T cell receptor signaling pathway

positive regulation of T cell activation

positive regulation of release of sequestered calcium ion into cytosol protein binding

mitochondrion

mitochondrion

mitochondrial inner membrane

protein targeting to mitochondrion

translocase of transport NM_001024853, inner NM_013896 1450019 Timm9 mitochondrial 12 ‐72224158 30056 12 12 C3 Mm.207767 19 protein transport ‐1.38667 0.000588 NM_001024853, membrane 9 NM_013896 homolog (yeast) membrane

mitochondrial intermembrane space protein transporter complex

protein import into mitochondrial inner membrane

metal ion binding

transmembrane transport molecular_function

actin binding

protein binding

cellular_component

5890253 Tns4 tensin 411‐98926992 NM_172564 217169 11 11 D Mm.262067 11 cytoplasm ‐0.723333 0.01114

cytoskeleton

apoptosis

biological_process

cell junction catalytic activity

serine‐type endopeptidase activity proteolysis tryptase 17 10.4 cM 17 1400347 Tpsab1 17 ‐25480189 NM_031187 17230 Mm.3301 28 1.62 0.009117 alpha/beta 1 A3.3 peptidase activity

serine‐type peptidase activity

hydrolase activity receptor activity

transmembrane receptor activity

extracellular region triggering receptor 6290358 Trem2 17 48485725 NM_031254 83433 17 17 C Mm.261623 34 plasma membrane ‐1.26333 0.000965 expressed on myeloid cells 2 signal transduction

membrane

integral to membrane extracellular region

basement membrane

extracellular space

endosome

transport

ion transport

iron ion transport

iron ion transport

cellular iron ion homeostasis

9 56.0 cM 9 ferric iron binding 1050193 Trf transferrin 9 ‐103111205 NM_133977 22041 Mm.37214 130 ‐1.25333 0.00066 F1‐F3 ferric iron binding

ferric iron transmembrane transporter activity

ferric iron transport

endocytic vesicle

endocytic vesicle

positive regulation of myelination metal ion binding

recycling endosome

SMAD protein signal transduction molecular_function tripartite motif‐ 6510068 Trim16 11 62633754 NM_053169 94092 11 11 B2 Mm.117087 13 cytoplasm ‐1.10667 0.000145 containing 16 biological_process molecular_function

nucleic acid binding

RNA binding

tRNA (guanine‐N2‐)‐methyltransferase activity

TRM1 tRNA cellular_component methyltransferase 8 39.0 cM 8 1.04E+08 Trmt1 8 87213145 212528 Mm.275720 15 ‐1.91333 0.00035 1 homolog (S. C3 tRNA processing cerevisiae) biological_process

methyltransferase activity

zinc ion binding

transferase activity

metal ion binding molecular_function

cellular_component

780022 Tspan15 tetraspanin 15 10 ‐61648143 NM_197996 70423 10 10 B4 Mm.416781 13 biological_process ‐0.676667 0.006488

membrane

integral to membrane molecular_function

cellular_component

membrane 1740538 Tspan8 tetraspanin 8 10 115254339 NM_146010 216350 10 10 D2 Mm.22270 15 ‐0.71 9.00E‐05 integral to membrane

negative regulation of blood coagulation

tetratricopeptide Mm.254979 7050446 Ttc33 15 5135559 NM_026213 67515 15 15 A1 12 binding ‐0.5 0.003003 repeat domain 33 Mm.438095 molecular_function

nucleus 80403536, U5 snRNP thioredoxin‐like 80403536, 18 50.0 cM 18 4120286 Txnl4a 18 NM_025299 27366 Mm.172411 16 ‐1.04333 0.00042 4A 80403584, E3 U5 snRNP 80403605 cytoplasm

biological_process protein binding

cytoplasm

vacuolar protein processing vacuolar protein 8 38.0 cM 8 Mm.296520 7050687 Vps35 8 ‐87784290 NM_022997 65114 19 transport ‐1.03333 0.000671 sorting 35 C3 Mm.391632 protein transport

membrane

integral to membrane molecular_function WD repeat 6020601 Wdr46 17 34077667 NM_020603 57315 17 17 B1 Mm.2437 12 cellular_component KO Only KO Only domain 46 biological_process molecular_function

cellular_component WD repeat 5570097 Wdr92 11 17111895 NM_178909 103784 11 11 A2 Mm.298132 13 ‐1.14 0.001609 domain 92 apoptosis

biological_process molecular_function WAP four‐ 1980528 Wfdc3 disulfide core 2 ‐164556725 NM_027961 71856 2 2 H3 Mm.330770 11 cellular_component ‐0.826667 0.007466 domain 3 biological_process

Werner syndrome 540687 Wrn ‐0.763333 0.000817 homolog nucleus

nucleus

nucleoplasm Wilms' tumour 1‐ ‐13163885, ‐ Mm.275521 1.05E+08 Wtap associating 17 13163885, ‐ NM_001113533 60532 17 17 A1 21 mRNA processing 0.72 9.00E‐05 Mm.481535 protein 13159664 cell cycle multicellular organismal development

RNA splicing Cytokine‐ cytokine cytokine activity receptor interaction

Chemokine extracellular region signaling pathway

extracellular space chemokine (C 1 87.0 cM 1 3800504 Xcl1 1 ‐166861778 NM_008510 16963 Mm.190 31 WT Only WT Only motif) ligand 1 H2.2 chemotaxis

chemotaxis

immune response

chemokine activity

release of sequestered calcium ion into cytosol binding

nucleus

1.06E+08 Xpo4 exportin 414‐58201107 57258 14 14 C3 Mm.202747 16 cytoplasm 0.526667 0.003115

transport

protein transport molecular_function yippee‐like 5 1.02E+08 Ypel5 17 73186043 NM_027166 383295 17 17 E2 Mm.277533 15 cellular_component 0.9 0.000371 (Drosophila) biological_process nuclear chromosome

regulation of cytokine production

nucleic acid binding

transcription corepressor activity

protein binding

intracellular

zinc finger and nucleus 5900397 Zbtb32 BTB domain 7 ‐31374699 NM_021397 58206 7 7 B2‐B3 Mm.116789 18 WT Only WT Only containing 32 transcription zinc ion binding

negative regulation of transcription

hemopoiesis

T cell proliferation

regulation of transcription

metal ion binding cellular_component

zinc ion binding

acyltransferase activity

membrane zinc finger, DHHC 1.05E+08 Zdhhc2 domain 8 41509168 70546 8 8 A4 Mm.34326 19 integral to membrane 0.706667 0.002056 containing 2 palmitoyltransferase activity

transferase activity

protein palmitoylation

metal ion binding molecular_function zinc finger protein 1.06E+08 Zfp862 6 48454337 58894 6 6 B2.3 Mm.31983 11 cellular_component 0.956667 0.00023 862 biological_process

278 Genes