Recombinant Human PPIL1/Cyclophilin-Like 1 Protein Catalog Number: PPL0901

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Recombinant human PPIL1/Cyclophilin-like 1 protein Catalog Number: PPL0901 PRODUCT INPORMATION Expression system E.coli Domain 1-166aa UniProt No. Q9Y3C6 NCBI Accession No. NP_057143 Alternative Names Peptidylprolyl isomerase (cyclophilin)-like 1, CYPL1, hCyPX, MGC678, PPIase, CGI-124, PPIL1, Peptidylprolyl isomerase (cyclophilin)-like 1, Peptidylprolyl isomerase (cyclophilin)-like 1 CGI124, Cyclophilin related gene 1, Peptidylprolyl isomerase (cyclophilin) like 1, peptidylprolyl isomerase like 1, rotamase0. PRODUCT SPECIFICATION Molecular Weight 19.3 kDa (174aa) confirmed by MALDI-TOF Concentration 1mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol Purity > 95% by SDS-PAGE Biological Activity Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP- PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. Tag His-Tag Application Enzyme Activity,SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Peptidylprolyl isomerase (cyclophilin) -like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans 1 Recombinant human PPIL1/Cyclophilin-like 1 protein Catalog Number: PPL0901 isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E. coli and purified by conventional chromatography techniques. Amino acid Sequence MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH General References Xu C., et al. (2006) J Biol Chem. 281(23):15900-8. Obama K., et al. (2006) Clin Cancer Res. 12(1):70-6. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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32-2709: PPIL1 Recombinant Protein Description Product Info Application
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-2709: PPIL1 Recombinant Protein Alternative Name : Peptidyl-Prolyl Cis-Trans Isomerase-Like 1,PPIL-1,CYPL1,hCyPX,MGC678,PPIase,CGI-124,PPIL1. Description Source : Escherichia Coli. PPIL1 Human Recombinant protein produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 174 amino acids (1-166) and having a molecular mass of 19.3 kDa. PPIL1 is fused to 8 amino acid His Tag at C-terminus and is purified by proprietary chromatographic techniques. PPIL1 belongs to the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a well conserved, ubiquitous family, members of which take an significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 protein increases the folding of proteins and catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. PPIL1 is a novel molecular target for colon- cancer therapy. Product Info Amount : 50 µg Purification : Greater than 95.0% as determined by SDS-PAGE. Content : PPIL1 solution containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol PPIL1 Human Recombinant although stable at 4°C for 1 week, should be stored desiccated below Storage condition : -18°C. Please prevent freeze thaw cycles. Amino Acid : MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH. Application Note Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
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In This Table Protein Name, Uniprot Code, Gene Name P-Value
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Frontiers Medicine Csa 2021
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Structural and Functional Insights Into Human Nuclear Cyclophilins
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Primepcr™Assay Validation Report
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