bioRxiv preprint doi: https://doi.org/10.1101/2021.03.21.435734; this version posted March 22, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license. Computational modeling of C-terminal tails to predict the calcium-dependent secretion of ER resident proteins Kathleen A. Trychta1,†, Bing Xie2,† , Ravi Kumar Verma2, Min Xu2, Lei Shi2,*, Brandon K. Harvey1,* 1 Molecular Mechanisms of Cellular Stress and Inflammation Unit 2 Computational Chemistry and Molecular Biophysics Section National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, 21224 † These authors have contributed equally to this work and share first authorship. * These authors share senior authorship. Correspondence:
[email protected] [email protected] Key Words: ER calcium, thapsigargin, KDEL receptor, ER retention sequence, exodosis Word Count: 4831 Figures: 4 Supplemental Tables: 2 Supplemental Figures: 3 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.03.21.435734; this version posted March 22, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license. Abstract The lumen of the endoplasmic reticulum (ER) has resident proteins that are critical to perform the various tasks of the ER such as protein maturation and lipid metabolism.