Insect Systematics & Evolution 50 (2019) 1-35 brill.com/ise Phylogeny and evolution of the genus Ctenocolum Kingsolver & Whitehead (Coleoptera, Chrysomelidae, Bruchinae), with the description of three new species Daiara Manfioa, Isaac Reis Jorgeb, Gael J. Kergoatc and Cibele Stramare Ribeiro-Costab aUniversidade Tecnológica Federal do Paraná (UTFPR), Campus Dois Vizinhos, Estrada para Boa Esperança, Km 04, Comunidade de São Cristóvão, 85660-000, Dois Vizinhos (PR), Brazil. E-mail:
[email protected] bLaboratório de Sistemática e Bioecologia de Coleoptera, Departamento de Zoologia, Universidade Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brazil. E-mail:
[email protected],
[email protected] cINRA - UMR CBGP (INRA, IRD, Cirad, Montpellier SupAgro), 755 Av. du campus Agropolis, 34988 Montferrier-sur-Lez, France. E-mail:
[email protected] Version of Record, published online 4 December 2017; published in print 2 January 2019 Abstract The seed beetle genus Ctenocolum Kingsolver & Whitehead is peculiar because its preferred host Lon- chocharpus Kunth (Fabaceae) is not preyed upon by other bruchine species. This study investigates the phy- logenetic relationships and evolution of this genus and of its species groups, while providing the description of three new species and of the male of C. biolleyi Kingsolver & Whitehead. To infer phylogenetic relation- ships, a character matrix of 40 morphological characters was assembled and analysed using both parsimony and Bayesian inference. Ancestral state estimations of host plant use and biogeography analyses were also performed. A total of 22 species were examined: 16 Ctenocolum species (including the three new ones) and six outgroup bruchine species (from genera Caryedes Hummel, Meibomeus Bridwell, Pygiopachymerus Pic and Pachymerus Thunberg).