NORTH-WESTERN JOURNAL OF ZOOLOGY 11 (1): 86-91 ©NwjZ, Oradea, Romania, 2015 Article No.: 141209 http://biozoojournals.ro/nwjz/index.html

First record of Dinychus bincheaecarinatus Hirschmann, Wagrowska-Adamczyk & Zirngiebl-Nicol in Romania: Notes on the morphology and and a contribution to the Dinychidae fauna of Romania (: : Uropodina)

Jenő KONTSCHÁN1,2

1. Department of Zoology and Ecology, Szent István University, H-2100, Gödöllő, Páter Károly str. 1., Hungary. 2. Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary. E-mail: [email protected]

Received: 30. June 2013 / Accepted: 01. September 2013 / Available online: 02. January 2015 / Printed: June 2015

Abstract. One female and one male specimen of the Dinychus bincheaecarinatus Hirschmann, Wagrowska- Adamczyk & Zirngiebl-Nicol, 1984 were collected for the first time in Romania. A new re-description of this species is given accompanied with first description of the legs. Notes on the differences between D. binchaeacarinatus Hirschmann, Wagrowska-Adamczyk & Zirngiebl-Nicol, 1984 and D. carinatus Berlese, 1903 are presented. Other new occurrences of the Romanian dinychid species are given with a list and a key to the Romanian species of the genus Dinychus.

Key words: Acari, Dinychidae, new records, new key, Romania.

Introduction collected by the following researchers: Csaba Csuzdi (CsCs), Edit Horváth (HE), Jenő Kontschán (KJ), András The Uropodina are one of the characteristic Orosz (OA), Victor V. Pop (VVP), György Sziráki (SzGy) and Zsolt Ujvári (UZs). The specimens were cleared in groups of soil dwelling Mesostigmata. Currently lactic acid and observed in deep and half covered slides, more than 2000 species have been discovered and with a scientific microscope. Illustrations were made with described, from the polar to the tropical regions of the aid of a drawing tube. Identified specimens were Earth (Lindquist et al. 2009). stored in alcohol and deposited in the Soil Zoology Col- Wiśniewski (1993) summarized the known lection of the Hungarian Natural History Museum. Uropodina species by countries. Romania was one Measurements are given in micrometers (µm). Width of of the intensely studied countries of Europe with idiosoma was taken at the level of coxae IV.

83 species presented in this work. Numerous spe- cies were later added to this list by Huţu & Results Călugăr (1994), Huţu (2000), Kontschán (2006), Constantinescu & Cristescu (2007), Kontschán & Dinychus bincheaecarinatus Hirschmann, Ujvári (2008), Constantinescu et al. (2011), Con- Wagrowska-Adamczyk & Zirngiebl-Nicol, 1984 stantinescu (2012) and Kontschán (2013a). (Figs 1-10) The genus Dinychus Kramer, 1886 is a charac- teristic one and the single European genus of the Material examined. One female and one male. family Dinychidae. It belongs to one of the most Romania, near village Cobeşti, beech forest with species-poor genera of the Uropodina mites. More small stream, from decayed tree, 26.X.2009, than 20 species are known from the Holarctic N46º6.544’, E22º21.590’ 337 m, leg. Csuzdi, Cs., (Wiśniewski 1993), the Neotropical region Kontschán, J., Popp, V.V. and Ujvári Zs. (Kontschán 2010), Tasmania (Błoszyk & Halliday Description of female. Length of idiosoma 600 1995) and the Indo-Malay region (Kontschán & µm, width 280 µm (n=1). Shape of idiosoma ob- Starý 2011). No recent Dinychus species are known long. from Africa. Dorsal aspect of idiosoma (Fig. 1): Marginal and

dorsal shields completely separated. All dorsal se-

Material and methods tae smooth and needle-like (ca. 19-24 µm). Mar- ginal setae similar in shape and length to dorsal The specimens investigated were found in the Collection setae. Posterior platelets bearing one pair of pilose of the Soil Zoology Department of the Hungarian Natural and two pairs of needle-like setae. Dorsal and History Museum (Budapest, Hungary). The samples were marginal shields covered by irregular and oval

Dinychus species of Romania 87

Figures 1-4. Dinychus bincheaecarinatus Hirschmann, Wagrowska-Adamczyk & Zirngiebl-Nicol, 1984 (female). (1) Dorsal view of body; (2) Ventral view of body; (3) Peritreme; (4) Ventral view of gnathosoma, tritosternum, palp and the first two segments of leg I.

pits. A characteristic ornamentation situated Prestigmatid part of peritremes long and with around central setae of dorsal shield. characteristic shape, post-stigmatid part absent Ventral aspect of idiosoma (Fig. 2): Sternal setae (Fig. 3). short, smooth and needle-like. St1 (ca. 40 µm) Tritosternum (Fig. 4) with narrow basis, tritos- placed near margin of sternal shield, St2 (ca. ternal laciniae subdivided into four branches, the 42µm) at level of central area of coxae II, St3 (ca. 84 lateral branches smooth, central ones with bulbi- µm) at level of anterior margin of coxae III and St4 form apical part. (ca. 77 µm) situated at level of anterior margin of Gnathosoma (Fig. 4): Corniculi horn-like, inter- coxae IV, St5 (ca. 65 µm) at level of posterior mar- nal malae smooth longer than corniculi and api- gin of coxae IV. Sternal shield covered by oval cally bifurcated. Hypostomal setae h1 long (ca. 45 pits. Ventral shield with alveolar and irregular or- µm) and smooth, h2 short (ca. 17 µm) and margin- namentation, all ventral setae smooth, needle-like ally serrate, h3 as long as h1 but marginally ser- and ca. 14-18 µm long. A preanal line situated an- rate, h4 (ca. 15 µm) short apically bifurcated. Che- terior to anal opening. Adanal setae and postanal licerae not clearly visible, fixed digit longer than seta similar in length and shape to ventral setae. movable digit. Ventral part of palp trochanter Genital shield scutiform and situated between bearing two serrate setae, other setae on palp coxae III and IV. Its anterior margin bearing wide smooth and needle-like. Epistome apically pilose. and spiny process and its surface covered by oval Legs (Figs 6-9): All legs bearing claws on tip of pits (Fig. 5). tarsi, but claw of leg I smaller and narrower than

88 J. Kontschán

Figures 5-10. Dinychus bincheaecarinatus Hirschmann, Wagrowska-Adamczyk & Zirngiebl-Nicol, 1984. (5) Intercoxal area of female; (6) Ventral view of leg I; (7) Ventral view of leg II; (8) Ventral view of leg III; (9) Ventral view of leg IV; (10) Intercoxal area of male.

others. All legs with smooth and needle-like setae, chus bincheaecarinatus Hirschmann, Wagrowska- except tarsi of legs II-IV, where robust and serrate Adamczyk & Zirngiebl-Nicol, 1984 as a junior setae can be found. synonymous name of the Dinychus carinatus Ber- Description of male. Length of idiosoma 580 lese, 1903, but he did not include in this work any µm, width 270 µm (n=1). Shape of idiosoma, or- information about why he thought that these two namentation and chaetotaxy of the dorsal part as species are conspecific. In Błoszyk’s (1999) men- in female. Sternal setae short and needle-like. St1 tioned paper, an illustration is presented about the placed near the anterior margin of sternal shield. D. carinatus species. The illustrated species differs St2 situated at level of the posterior margin of from the German (Karg 1989), Hungarian coxae II, St3 at level of the anterior margin of (Kontschán 2004), Slovakian (Mašán 2001) and coxae III, St4 at level of anterior margin of genital Romanian species in the ornamentation of the shield. St5 placed at level of the posterior margin sternal shield (strongly ornamented in the Ger- of coxae IV. Genital shield oval and situated be- man, Hungarian, Slovakian and Romanian speci- tween coxae IV (Fig. 10). Ventral setae and orna- mens and scarcely ornamented in Błoszyk’s mentation similar to those of female. specimens) and in the shape of the anterior proc- Remarks. When Hirschmann et al. (1984) de- ess of the female genital shield (narrow in scribed this species they mentioned that D. Błoszyk’s specimens and wide in the others). Un- bincheaecarinatus differs from D. carinatus in the fortunately Błoszyk (1999) did not present the shape of the peritremes (poststigmatid part absent most important character (absence/presence of the in D. bincheaecarinatus and present in D. carinatus). poststigmatid part of peritremes) in this work; Later Błoszyk (1999) presented the name of Diny- therefore I cannot agree with his opinion. On basis Dinychus species of Romania 89 of the earlier mentioned characters, I am sure that Dinychus inermis (C. L. Koch, 1841) these two species are very similar, but they are not New records. - conspecific. Previous records from Romania. Region of Moldova River (Huţu, 1974), Danube Delta Bio- List of the Romanian species of the family sphere Reserve (Huţu 1993, Huţu & Călugăr 1994). Dinychidae Distribution. Europe.

Family Dinychidae Berlese, 1916 Dinychus perforatus Kramer, 1886 Genus Dinychus Kramer, 1886 New records. Romania, near Lacul Sfânta Ana, 600m, from moss, 01.VIII.1999, OA. Romania, Ic Dinychus arcuatus (Trägardh, 1943) Ponor, Sphagnum bog, from moss, N46º37.417’, New records. Romania, Someşul Cald, pasture, E22º48.218’ 1065 m, 25.X.2009, CsCs-KJ-VVP-UZs. from soil, 450 m, 24.VII.2009, CsCs. Romania, Previous records from Romania. Region of Sovata, 1000 m, from moss from tree, 12.VIII.2010, Moldova River (Huţu, 1974), Maramureş SzGy. Someşul Cald, near lake Tarniţa, mixed (Kontschán & Ujvári 2008). beech forest, 610 m, from leaf litter, 22.VII.2009, Distribution. Europe. CsCs, Zorenii de Vale, mixed forest fragment, 46°47,532’, 24°03,795’, 411 m, from decayed tree, Dinychus sellnicki Huţu, 1973 03.XI.2007, CsCs-KJ-VVP. New records. - Previous records from Romania. Maramureş Previous records from Romania. Agapia (Huţu, (Kontschán 2006, Kontschán & Ujvári 2008). 1973). Distribution. Europe. Distribution. Europe. Remark. This is an endemic species. Dinychus bincheaecarinatus Hirschmann, Wagrowska-Adamczyk & Zirngiebl-Nicol, 1984 Dinychus septentrionalis (Trägardh, 1943) New records. Romania, near village Cobeşti, beech New records. - forest with small stream, from decayed tree, Previous records from Romania. Maramureş N46º6.544’, E22º21.590’ 337 m, 26.X.2009, CsCs-KJ- (Kontschán 2006). VVP-UZs. Distribution. Europe. Previous records from Romania. - Dinychus undulatus Sellnick, 1945 Distribution. Europe. New records. Romania, Turda, near Cheile Turzii, Remark. This is the first record from Romania. from moss, 20.IX.2000, HE. Dinychus carinatus Berlese, 1903 Previous records from Romania. Region of New records. Danube Delta Biosphere Reserve Moldova River (Huţu, 1974). (Huţu & Călugăr 1994). Distribution. Europe. Previous records from Romania. This species is Dinychus woelkei Hirschmann & Zirngiebl-Nicol, presented without exact localities, only known 1969 from Wiśniewski’s (1993) list. New records. Romania, near village Cobeşti, beech Distribution. Europe. forest with small stream, from decayed tree, Dinychus feideri Huţu, 1973 N46º6.544’, E22º21.590’ 337 m, 26.X.2009, CsCs-KJ- New records. - VVP-UZs. Previous records from Romania. Strunga (Huţu, Previous records from Romania. This species is 1973). presented without exact localities, only known Distribution. Romania and Slovakia. from Wiśniewski’s (1993) list. Distribution. Central-Europe.

Key to the females of the Romanian Dinychus species

1. Posterior platelets on dorsal idiosoma with three pairs of setae 2 ― Posterior platelets on dorsal idiosoma with four pairs of setae 3 2. Posterior platelets on dorsal idiosoma with three pairs of pilose setae 4 ― Posterior platelets on dorsal idiosoma with one pair of pilose and two pairs of smooth setae 5 3. Genital shield narrower (length: width 1:1.2), J4 as long as J5 D. sellnicki ― Genital shield wider (length: width 1:1), J4 shorter than J5 D. septentrionalis 4. Genital shield linguliform, surface of genital shield smooth D. feideri 90 J. Kontschán

― Genital shield scutiform, surface of genital shield with oval pits D. woelkei 5. Poststigmatid part of peritremes absent D. bincheaecarinatus ― Poststigmatid part of peritremes present 6 6. Whole peritremes undulated D. undulatus ― Peritremes not undulated 7 7. Poststigmatid part of peritremes straight 9 ― Poststigmatid part of peritremes with several bends 8 8. Genital shield ornamented D. carinatus ― Genital shield smooth D. inermis 9. Basal line of genital shield situated at level of central area of coxae IV, posstigmatid part of peritremes reach to central area of coxae IV D. arcuatus ― Basal line of genital shield situated at level of anterior margin of coxae IV, posstigmatid part of peritremes reach to posterior margin of coxae IV D. perforatus

Discussion Mesostigmata) w Polsce. I. Uropodina lasów grądowych (Carpinion betuli). Kontekts, Poznań, 245p. Błoszyk, J., Halliday, B. (1995): A new species of Dinychus Kramer Currently 30 Dinychus species are described from from Tasmania (Acarina: Dinychidae). Journal of the Australian all regions of Earth, only one species is presented Entomological Society 34: 187-191. from South-East Asia (Kontschán & Starý 2010), Constantinescu, I.C. (2012): A new species of myrmecophile from Romania (Acarina: Anactinotrichida: Uropodina). North- one from North-America (Anthias-Binche et al. Western Journal of Zoology 8(1): 22-26. 1989), four from South-America (Kontschán 2011) Constantinescu, I.C., Cristescu, C. (2007): Studies on the trophic and the other species are presented from Europe. preferences of certain Uropodina mites (Acarina: Anactinotrichida, Uropodina) for some taxa of imperfect fungi. The ten listed Dinychus species from Romania are Analele ştiinţifice ale Universităţii “Al. I. Iaşi, s. Biologie animală many compared with the neighboring countries, 53: 83-87. with only 2-10 listed species (one species from Constantinescu, I.C., Ivan, O., Călugăr, A., Markó, B. (2011): Mite fauna of ant nests – comparative study of fauna in the Argeş Ukraine (Wiśniewski 1993), three species from River Basin. Travaux du Muséum National d’Histoire Naturelle Bulgaria (Kontschán 2013b), six from Hungary «Grigore Antipa» 54(2): 327-342. (Kontschán 2008) and ten from Slovakia (Mašán Hirschmann, W., Wagrowska-Adamczyk, B., Zirngiebl-Nicol, I. (1984): Gangsystematik der Parasitiformes Teil 467. Adulten- 2001)). One species (D. sellnicki) only occurs in Gruppen und Adultenbestimmungstabelle von 20 Dinychus- Romania, so it can be considered an endemic spe- Arten. Neuzeichnung von Dinychus tetraphyllus Berlese, 1903 cies. Other species of this genus can be found in (Dinychini, Uropodinae). Acarologie. Schriftenreihe für Vergleichende Milbenkunde 31: 127-130. numerous countries of Europe. Huţu, M. (1973): Gangsystematik der Parasitiformes. Teil 145. Zur Kenntnis der Uropodiden-Fauna Rumäniens. neue Uropodiden- Arten der Gattungen Trachytes Michael 1894, Dinychus Kramer 1886 und Trachyuropoda (Berlese 1888) Hirschmann u. Zirngiebl- Nicol, 1961 nov. comb. Acarologie. Schriftenreihe für Acknowledgements. I am very grateful to my colleagues Vergleichende Milbenkunde 19: 45-51. and friends, who collected mite material in Romania. This Huţu, M. (1974): Contribuţii la cunoaşterea faunei de Uropodide research was supported by the European Union and the (Acari: Parasitoformes) din zona Cursului superior al Răului State of Hungary, co-financed by the European Social Moldova. Studii şi cercetări de Biologie 25(2): 83-88. Fund in the framework of TÁMOP 4.2.4. A/-11-1-2012- Huţu, M. (1993): Spectrul ecologic al faunei de Uropodide (Acarina: 0001 ‘National Excellence Program’. The research was Anactinotrichida: Uropodina) din Rezervaţia Biosferei Delta Dunării. Anale Ştiinţifice ale Institutului Delta Dunării 1993: partly supported from the infrastructural point of view 109-114. (collection equipment and used materials in the Huţu, M. (2000): Zwei neue Trachytes-Arten (Uropodina: investigation) by the Hungarian Scientific Research Fund Trachytidae) aus Rumänien. Abhandlungen und Berichte des (OTKA 72744, 100369). Naturkundemuseums Görlitz 72(2): 239-252. Huţu, M., Călugăr, A. (1994): Uropodid fauna (Acarina: Anactinotrichida: Uropodina) in the Danube Delta Biosphere Reserve and it surroundings zones. Analele Ştiinţifice

Institutului Delta Dunării 1994(3): 51-57. References Karg, W. (1989): Acari (Acarina), Milben Unterordnung

Parasitiformes (Anactinochaeta), Uropodina Kramer, Athias-Binche, F., Błoszyk, J., Olszanowski, Z. (1989): Dinychus Schildkrötenmilben. Die Tierwelt Deutschlands 67: 1-203. ruseki n. sp. (Acari: Uropodina) from Canada, with remarks on Kontschán, J. (2004): New and rare Uropodina (Acari: the habitats and distribution of the members of the Mesostigmata) species from Hungary. Annales Historico– genus Dinychus. Canadian Journal of Zoology 67(6): 1482-1488. Naturales Musei Nationalis Hungarici 96: 273-278. Błoszyk, J. (1999): Geograficzne i ekologiczne zróżnicowanie Kontschán, J. (2006): Mesostigmatid mites from Maramureş zgrupowań roztoczy z kohorty Uropodina (Acari: (Romania) (Acari: Mesostigmata: Uropodina et Gamasina: Dinychus species of Romania 91

Zerconidae, Macrochelidae, Epicriidae, Eviphididae et Kontschán, J., Ujvári, Zs. (2008): Mesostigmatid mites from Parasitidae). Studia Universitatis Vasile Goldis, Arad 17: 53-57. Maramureş (Acari: Mesostigmata) I. Studia Universitatis Vasile Kontschán, J. (2008): Magyarország korongatkái (Acari: Goldis, Arad 18: 347-357. Mesostigmata: Uropodina). (Turtle mites of Hungary (Acari: Lindquist, E.E., Krantz, G.W., Walter, D.E. (2009): Order Mesostigmata: Uropodina). Állattani Közlemények 93(1): 3-15. Mesostigmata. pp. 124-232. In: Krantz, G.W., Walter, D.E. (eds.), Kontschán, J. (2010): Dinychus empalmensis n. sp. from Costa Rica A manual of acarology. Third edition. USA, Texas University with notes on the Neotropical Dinychus species (Acari: Press. Uropodina: Dinychidae). International Journal of Acarology Mašán, P. (2001): Mites of the cohort Uropodina (Acari, 36(5): 413-417. Mesostigmata) in Slovenska. Annotationes Zoologicae et Kontschán, J. (2013a) Notes to the morphology and the Romanian Botanicae 223: 1-320. distribution of Uroobovella hungarica Hirschmann & Zirngiebl- Wiśniewski, J. (1993): Gangsystematik der Parasitiformes. Teil 549. Nicol, 1962 (Acari: Uropodina). Opuscula Zoologica Budapest Die Uropodiden der Erde nach Zoogeographischen Regionen 44(1): 91-95. und Subregionen geordnet (Mit Angabe der Lande). Acarologie. Kontschán, J. (2013b): Uropodina mites of the Balkan Peninsula Schriftenreihe für Vergleichende Milbenkunde 40: 221-291. (Acari: Mesostigmata). Opuscula Zoologica Budapest 44 (Suppl. 1): 97–131. Kontschán, J., Starý, J. (2011): Uropodina mites from Vietnam (Acari: Mesostigmata). Zootaxa 2807: 1-28.