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Developing Technical Skills for Stem-Cell Research

Developing Technical Skills for Stem-Cell Research

2 | NATUREJOBS | 31 MAY 2012 SPOTLIGHT ON STEM- RESEARCH NATUREJOBS “More and more and people “More in my know least at lab lines of program.” how to write afew Fédérale de Lausanne Didier Trono, École Polytechnique when researchers at the Karolinska the at researchers when 2011 in shown was technology this of application encouraging An scafolding. 3-D is attention to when apply them. and to use factors growth which refning involves method another develop; cells stem many which in conditions hypoxic the reproduce to mixtures gas exotic using is approach of this One example (NCS). Research Cell Stem for Center Norwegian of the director Glover,Joel says cells," of stem environments natural the mimic to trying have been scientists recent years, “In refned. being constantly is cells stem culturing for media growth optimal Te growth Nurturing eforts? your you focus should where So of . edge cutting the at to stay of techniques range adiverse with themselves arm investigators Stem-cell roles. supporting valuable many are there and feld, the in skills important most one of the is types cell specialized derive and cells stem how toculture Knowing a mark. to make ways many but are there Sciences, Life Healthcare GE at technologies development of cell and of research head role of global to the him brought has research passion.” and dedication takes that It’s need. something they out what fguring and cells inside of getting “a and mindset says, he thumb”, to have “a green you need cells, stem culturing To in Stephen Minger. succeed researcher stem-cell leading says to grow, difcult are Both orchid? it takes to succeed in this dynamic field. what cells? out Find stem with working in Interested researchfor stem-cell Developing skills technical RESEARCH ONSPOTLIGHT STEM-CELL HOW IS Another area attracting alot of attracting area Another on stem-cell take Minger’s a like an an like cell a stem College London Schoolof Medicine,must abidebystrict protocols. Researchers working onstem cellsfor clinicaluse,suchasthisscientist at King’s modifcation of cells. A commonly commonly A cells. of modifcation genetic the involves research stem-cell of proportion major A The role of genetics increasingly valuable. become will excitationtwo-photon microscopy of 3-D techniques imaging such as behave in a3-D context, knowledge scientists study how stem cells potential. He predictsthat more as optimistic about the technology’s Center (MSKCC) in New York is Memorial Sloan–Kettering the at Center for Stem Cell future. Lorenz Studer, director of for stem-cell researchers in the 3-D patterns prove —might useful printers into to cells deposit specifc inkjet printing using — organ NCS’scells. that Glover suggests with own his stemscafold seeded tracheaa new made from a3-D Institute in Sweden apatient gave signals. Familiarity with new new with Familiarity signals. to external response cell’s the and diferentiation, tissue homeostasis cell infuence changes epigenetic how determine to trying are stem-cell research. Researchers in importance of growing — is DNA in changes not involving function in changes heritable advantageous. is protocols relevant the with experience so — hazardous be can lentiviruses recombinant — level biosafety enhanced an require may lab the in vectors viral Working with Lausanne (EPFL) in Switzerland. de Fédérale Polytechnique École at Sciences of Life School the at dean and Trono, professor Didier says research,” stem-cell of workhorses really “Tey're lentiviruses. as such vectors viral uses cells stem into to deliver technique used — the study of study —the Epigenetics ADVERTISEMENT FEATURE

KING’S COLLEGE LONDON STEMCELL TECHNOLOGIES INC ADVERTISEMENT FEATURE you’re for; looking appearances what fnding doesn’t guarantee alone But sorting skill. a crucial fuorescent-tagged antibodies — is using cells — identifying fow cytometry as such techniques via populations purifed into them sorting cultured, and prepared are cells stem Once Sorting and studying DNAfragments. resulting of the sequencing with ChIP combines which ChIP-seq, and technology, microarray with (ChIP) immunoprecipitation chip, which combines chromatin include ChIP-on- technologies Trono. says feld," the Epigenetics arevolution in in usher helped has epigenetics analyzing for techniques high-throughput of arrival “Te answers. the fnd to needed be will technology company Stemcell Technologies' research facilityinVancouver, . Project name Top 10stem-cell research projects fundedbyUSNational Institutes of Health(NIH)in2011 US stem-cell research funding Source: NIH Source: Mechanisms andbiologicalconsequences of thenuclearreceptor CARactivation Transcriptional functionsof nuclearreceptorsin cancercells Blood &Marrow Transplant ClinicalTrials Network Data Coordinating Center Growth control inmultiple myeloma Center for CellCircuits Adult stem cellsfor of visualdisorders Causal transcriptional consequences of humangenetic variation Research oninhalation of environmental chemicals KOMP phaseIImouseproduction andcryopreservation Global annotation of regulatory elements inthehumangenome Cell therapy for diabetic peripheral neurovascular complications

technique, frst described by described frst technique, Te cells. of stem a population of characteristics the to analyze anovel way researchers — ofers cytometry fow on variation a — microscopy. confocal does as cells, in expressed are of interest how analyze researchers helps also cytometry Image release.” hormone of pattern their or electrophysiology of their as such cells, of derived analysis functional careful additional need You do. anything do don't just but they neurons, like [make] and look them cells stem adult take you can “For instance, Glover of NCS. says of acell,” markers and morphology the just “You havemore at to look than form. its as well as function cell’s a to study It’s necessary research. stem-cell in deceiving be can A process mass cytometry called cells and restorative neurology neurology restorative and cells established a lab dedicated to stem Sweden recently in Center Cell Stem Lund Te globe. the across up ramping are direction this in into clinical . Eforts science basic to turn is researchers stem-cell for goal A major Into the clinic research. of stem-cell implications broader the help assess all can biology systems and behaviour animal of surgery, Experience March. adds integrate,” how they help you monitor can imaging (PET) tomography emission positron or ultrasound enhanced contrast- as such techniques cells, the you engraf “If organisms. living in cells derived and cells of stem behaviour the understanding in benefcial be can (ICVBM). Medicine and Biology Vascular for Center Indiana of the director March, Keith says toolbox, researcher’s to astem-cell addition It’s but new signifcant arelatively atime. at features afew measure only can fow cytometry based fuorescence- conventional whereas cells, inside and surface on the located of features dozens measure to simultaneously scientists allows approach Tis spectrometer. mass by a analyzed parts constituent their and vaporized are cells Te of fuorescentmolecules. instead isotopes of rare-earth metals antibodies with various tagged 2011, May in uses colleagues their and of Medicine School researchers at Stanford University Knowledge of animal physiology physiology of animal Knowledge Recipient organisation NIH NIH Medical College of Wisconsin University of Arkansas for MedicalSciences Broad Institute Scripps Research Institute Harvard University (MedicalSchool) Alion ScienceandTechnology Corporation University of Davis Hudson-Alpha Institute for Biotechnology Emory University says Dusko Ilic, senior lecturer in in lecturer senior Ilic, Dusko says correct,” is this evidence written you have that and sterile, was everything that temperature, wrong the kept at or been expired has no reagent that sure be —you must traceable to be has body. “Everything human the into to go allowed is what regarding (GCPs) regulations — practices (GMPs) clinical employ good and good manufacturing practices to adhere must cells stem grade system. patient'sthe immune by rejected not be would that transplants for cells stem specifc patient- help develop could that a strategy oocytes, unfertilized nuclei to adult by adding cells pluripotentgenerated stem have Foundation Cell Stem host. amouse in hepatocytes human on infection C and B to model the efect ofhepatitis iPSCs using are Connecticut in Center Yale the Cell at Stem and a team disease cardiovascular to model used (iPSCs) be can cells stem pluripotent induced have found human that California in Medicine Regenerative and Biology Cell Stem for Institute Stanford the at researchers Atlantic, of side the other the On . of breast cause the help unravel could that fnding a cells, stem of long-lived types diferent contains gland mammary the that demonstrated Belgium in Université Libre de Bruxelles the at scientists while cells, stem ’s neural adult the to study Scientists working with clinical- with working Scientists York New the at Scientists Amount (US$) $3.3m $3.4m $3.5m $3.7m $3.7m $3.8m $3.8m $3.9m $4.0m $4.1m $6.1m ❯❯

3 | NATUREJOBS | 31 MAY 2012 SPOTLIGHT ON STEM-CELL RESEARCH NATUREJOBS 4 | NATUREJOBS | 31 MAY 2012 SPOTLIGHT ON STEM-CELL RESEARCH NATUREJOBS says Minger of GE Healthcare. Healthcare. of GE Minger says week,” every of cells amount required the and results reproducible out very to churn standard operating procedure “We on to a level. lock viable to acommercially cells stem of production up the to scale crucial.” are skills organizational and planning “Proper he says. fow diagrams,” makes who next, one to the step from carefully very thinks that type “You the to be manner. need controlled ahighly in times multiple aprocedure to repeat able be you must and MSKCC, of Studer says personality”, meticulous “extremely an conditions.” with complying we are that checking inspections government are there And forms. sign and have to witness you both people; done by two to be has “All work United Kingdom. the in of Medicine School London College King’s at science stem-cell Strict controls are also necessary necessary also are controls Strict To you need GMPs follow Stemcell Technologies. “You Technologies. have Stemcell as such company a biotechnology at use researchers that techniques and instruments reagents, the developing is research cell-related hecome says. up with,” consistently you can something or noise random just are results your “You if to know want important. also is analysis that understanding statistical adds Minger of program.” lines afew how to write least at know my lab more in “More people and EPFL’s says edge,” an Trono. you give defnitely that skills are “Tese research. stem-cell in invaluable become has biology computational and in experience mean sets data generate massive that techniques high-throughput in advances of biology, felds many in As Technically minded entities.” biological unstable inherently on we're working — challenging “It's extremely An alternative avenue stem- into alternative An BSFDVSSFOUMZTFFLJOH 1I%T.4DT XJUINPTUPUIFSTIPMEJOHB#4DPSFOHJOFFSJOHEFHSFF(SPXJOHSBQJEMZ XF 8FIBWFBQQSPYJNBUFMZTDJFODFPSJFOUFEFNQMPZFFTHMPCBMMZ JODMVEJOH CZPVSDVTUPNFSTUPQSPWJEFPVUTUBOEJOHQSPEVDUT UFDIOJDBMTVQQPSUBOEUSBJOJOH QBTTJPOGPSRVBMJUZ XFTFFPVSTFMWFTTJNQMZBTi4DJFOUJTUT)FMQJOH4DJFOUJTUTwTUBOEJOH JO DFOUSFT EJTUSJCVUJPO QMVT PGmDFT 7BODPVWFS 4FBUUMF (SFOPCMFBOE4JOHBQPSF%SJWFOCZPVSMPWFPGTDJFODFBOEPVS SFHJPOBM NBOZ PVS WJB DPVOUSJFT  UIBO NPSF DSFBUFOPWFM VTFGVM TUBOEBSEJ[FEQSPEVDUTPGVOGBJMJOHRVBMJUZBOEEFMJWFSUIFNUP DFMM NFEJB  DVMUVSF TFQBSBUJPOQSPEVDUT JOTUSVNFOUT BODJMMBSZSFBHFOUTBOEDPOUSBDUBTTBZTFSWJDFT8F DFMM PVS PO SFMZ BOE XIP UIPTF NFEJDJOF BNPOH BSF SFHFOFSBUJWF SFTFBSDI UIFSBQZ DBODFS  DFMMVMBS JNNVOPMPHZ  DFMM  TUFN QFSGPSNJOH 4DJFOUJTUT XPSME UIF BSPVOE SFTFBSDI TDJFODFT MJGF MFBEJOHFEHF QPXFS IFMQT UIBU 7BODPVWFS JO CBTFE DPNQBOZ CJPUFDIOPMPHZ QSJWBUFMZPXOFE B JT *OD 5FDIOPMPHJFT 45&.$&-- "DPNQMFUFMJTUPGDBSFFSPQQPSUVOJUJFTJTBWBJMBCMFBU #PTUPOBOE/FX:PSL t5FDIOJDBM4BMFT3FQSFTFOUBUJWFT $FMM4FQBSBUJPO/PSUI$BSPMJOB  t5FDIOJDBM4BMFT3FQSFTFOUBUJWFT .FEJB/PSUI$BSPMJOBBOE/FX:PSL t.FTFODIZNBM4DJFOUJTU7BODPVWFS t*OEVDFE1MVSJQPUFOU4UFN$FMM4DJFOUJTU7BODPVWFS ranges from DNA sequencing, sequencing, DNA from ranges Tis companies. specialized to outsourced are research cell of MSKCC. Studer says cells,” stem from of neurons diferentiation basic do also lab our at —technicians rule the but that's not necessarily of cells, diferentiation out the carry usually “Postdocs blurred. become can lines the research, stem-cell In protocols. set follow who doctorates without secure grants, and technicians and research conduct who PhDs with scientists between divided typically are tasks labs, many In Division of labour Vancouver,in Canada. Technologies’ headquarters development Stemcell at and of research vice-president senior Terry says Tomas, types,” cell and of cells biology of the knowledge have good and a technique, to develop troubleshooting at good and creative resourceful, to be Increasingly, aspects of stem- aspects Increasingly, www.stemcell.com/careers responsibility for contentresponsibility no have staf editorial Nature science. cutting-edge and a vital of forefront the at to be want who researchers inquisitive meticulous, for abound opportunities feld, stem-cell the in of discovery ahead.” help put us will that techniques, new or develop methodologies existing retool we can “If discipline: scientifc unrelated an from technology or by atechnique inspired ofen are colleagues his he and says Institute, Cell Stem Harvard the at education of director faculty Lensch, William M. feld. to enter the potential the of researchers range wide a gives research stem-cell in services. GCP and GMP provide help also can groups research other with Collaborations March. ICVBM’s says studies”, preclinical “entire to analysis bioinformatics and characterization cell With the myriad strands strands myriad the With of discovery pace fast Te ADVERTISEMENT FEATURE NW223681R  ■ OCTOBER10-11, 2012 SEVENTH ANNUAL TRANSLATIONAL SEVENTH STEM CELL RESEARCH CONFERENCE CONFIRMED SPEAKERS & CHAIRS INCLUDE www.nyscf.org/conference |[email protected] For more information andtoregister visit AT THE ROCKEFELLER UNIVERSITY, NEW YORK CITY Gordon Keller, PhD Rudolf Jaenisch, MD Matthias Von Herrath, MD Samuel Gandy, MD, PhD , PhD Paul S. Frenette, MD Dieter Egli, PhD Yuval Dor, PhD Peter J. Coffey, DPhil Jeffrey Bluestone, PhD , PhD PaolaPhD Arlotta, Thomas Zwaka, MD PhD Amy Wagers, Lorenz Studer, MD Hans Snoeck, MD, PhD Hans Schöler, PhD Shahin Rafii, MD Plath,Kathrin PhD Thomas Perlmann, PhD Majeti,Ravindra MD, PhD Ruth Lehmann, PhD MD Joanne Kurtzberg, NW223449R 2012. Interviews The ([email protected]). Questions addressed be can to IMB’s Director, Christof Niehrs For information further IMB, on please www.imb-mainz.de. visit letters of recommendation to to sent be [email protected]. quoting reference the mainz.de, #CNRGL03. Please arrange also for 2 your researchinterests futureand research toplans personnel@imb- To apply, please your send coverletter, concise a and CV description of e.g. for to wishing individuals obtain Habilitation. German a obligations, teaching but are opportunities available at University, the budget for consumables. and personnel There are teaching no attractive an include salary, generous start-up a 5-year and funds are for5 years initially renewed.be can and Recruitment packages advanced mass astrong and publication record. Positions collaborative candidates innovative an with research profile in Scientists at are IMB supported by strong Core Facilities. We seek repair,DNA development interfaces the and between these fields. atProjects institute the are interdisciplinary focus and epigenetics, on Foundation byIngelheim funded 100M Euro. with Boehringer the integrated and of Mainz into community. scientific its generouslyis IMB research center of excellence situated on the campus of the University The Details regarding thefellowship are • • Candidates must have completed PhD Israeli• citizenship Candidates m Candidates Institute of Molecular (IMB) Biology research institution. host U.S. inthe position postdoctoral yearfinal study of University/Medical intheir orbe School Sciences a and/or MD/PhD degrees in t Gruss Post-Doctoral Lipper Research The EGL Charitable Foundation Fellowship Program invites you the invites to apply to will take placewill on Mainz in Group Leader t a at www.eglcf.org n accredited Israeli ust have been awarded a (#CNRGLO3) Eligibility is recruitingis a deadline for applications The InstituteThe of Molecular funded by funded Boehringer the Ingelheim FoundationIngelheim Biology gGmbH Biology Mainz, Germany,Mainz, a new is 24-25 September 2012. h e Biomedical available is 31 July NW224959R W224996R

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