Anti-TSC22D3 / GILZ Antibody (ARG58471)

Product datasheet [email protected]

ARG58471 Package: 100 μl anti-TSC22D3 / GILZ antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes TSC22D3 / GILZ

Tested Reactivity Hu, Ms, Rat

Tested Application ICC/IF, IHC-P, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name TSC22D3 / GILZ

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 1-79 of Human TSC22D3 / GILZ (NP_001015881.1).

Conjugation Un-conjugated

Alternate Names TSC-22R; hDIP; Protein DIP; DSIPI; TSC22 domain family protein 3; TSC-22-like protein; Glucocorticoid- induced leucine zipper protein; TSC-22-related protein; GILZ; Delta sleep-inducing peptide immunoreactor; DIP; DSIP-immunoreactive peptide

Application Instructions

Application table Application Dilution

ICC/IF 1:50 - 1:200

IHC-P 1:50 - 1:200

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control 293T

Calculated Mw 15 kDa

Observed Size 15 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

www.arigobio.com 1/3 Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TSC22D3

Gene Full Name TSC22 domain family, member 3

Background The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Function Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 inhibits myogenic differentiation and mediates anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG (By similarity). [UniProt]

Cellular Localization Cytoplasm, Nucleus. [UniProt]

Images

ARG58471 anti-TSC22D3 / GILZ antibody ICC/IF image

Immunofluorescence: U2OS cells stained with ARG58471 anti- TSC22D3 / GILZ antibody.

ARG58471 anti-TSC22D3 / GILZ antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG58471 anti-TSC22D3 / GILZ antibody at 1:100 dilution.

www.arigobio.com 2/3 ARG58471 anti-TSC22D3 / GILZ antibody WB image

Western blot: 25 µg of 293T cell lysate stained with ARG58471 anti- TSC22D3 / GILZ antibody at 1:1000 dilution.