DOCSLIB.ORG

Discovery of Osmotic Sensitive Transcription Factors in Fish Intestine Via a Transcriptomic Approach

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Discovery of Osmotic Sensitive Transcription Factors in Fish Intestine Via a Transcriptomic Approach Wong et al. BMC Genomics 2014, 15:1134 http://www.biomedcentral.com/1471-2164/15/1134 RESEARCH ARTICLE Open Access Discovery of osmotic sensitive transcription factors in fish intestine via a transcriptomic approach Marty Kwok-Shing Wong1*, Haruka Ozaki2, Yutaka Suzuki2, Wataru Iwasaki1,2,3 and Yoshio Takei1 Abstract Background: Teleost intestine is crucial for seawater acclimation by sensing osmolality of imbibed seawater and regulating drinking and water/ion absorption. Regulatory genes for transforming intestinal function have not been identified. A transcriptomic approach was used to search for such genes in the intestine of euryhaline medaka. Results: Quantitative RNA-seq by Illumina Hi-Seq Sequencing method was performed to analyze intestinal gene expression 0 h, 1 h, 3 h, 1 d, and 7 d after seawater transfer. Gene ontology (GO) enrichment results showed that cell adhesion, signal transduction, and protein phosphorylation gene categories were augmented soon after transfer, indicating a rapid reorganization of cellular components and functions. Among >50 transiently up-regulated transcription factors selected via co-expression correlation and GO selection, five transcription factors, including CEBPB and CEBPD, were confirmed by quantitative PCR to be specific to hyperosmotic stress, while others were also up-regulated after freshwater control transfer, including some well-known osmotic-stress transcription factors such as SGK1 and TSC22D3/ Ostf1. Protein interaction networks suggest a high degree of overlapping among the signaling of transcription factors that respond to osmotic and general stresses, which sheds light on the interpretation of their roles during hyperosmotic stress and emergency. Conclusions: Since cortisol is an important hormone for seawater acclimation as well as for general stress in teleosts, emergency and osmotic challenges could have been evolved in parallel and resulted in the overlapped signaling networks. Our results revealed important interactions among transcription factors and offer a multifactorial perspective of genes involved in seawater acclimation. Keywords: Transcriptome, Fish osmoregulation, Intestine, Seawater acclimation, Transcription factors, CEBPB, CEBPD, SGK1, TSC22D3 Background to maintain body fluid homeostasis in both FW and SW Osmoregulation is an important topic in fish physiology. [2,3]. SW teleosts drink copiously and the gastrointes- Bony fishes maintain their body fluid osmolality approxi- tinal tract is responsible for water absorption to com- mately one-third that of seawater (SW) and therefore pensate for the water loss by osmosis [4,5]. Although the they constantly lose water and gain ions in SW but gain intestine is an internal organ, its lumen directly contacts en- water and lose ions in fresh water (FW). Osmoregulation vironmental water upon drinking in teleost fishes. Osmosen- consumes a high proportion of daily energy expenditure sing in fish is accomplished by a combination of sensors in in teleosts as they either actively excrete excess ions in the central nervous system and peripheral osmoregulatory SW or take up ions in FW against the respective con- epithelia such as gill, nasal cavity, and intestine [6]. A reflex centration gradients [1]. The gills, kidney, and intestine inhibition in drinking was demonstrated in eel intestine in − are major osmoregulatory organs and play different roles response to Cl ions (but not Na+) in ingested fluid, indicat- − ing the presence of a Cl specific sensor in eel intestine [7]. * Correspondence: [email protected] Euryhaline fishes that are able to acclimate in both 1Atmosphere and Ocean Research Institute, The University of Tokyo, Tokyo, Japan FW and SW transform their intestines dramatically to Full list of author information is available at the end of the article fulfill the appropriate osmoregulatory roles. When the © 2014 Wong et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Wong et al. BMC Genomics 2014, 15:1134 Page 2 of 13 http://www.biomedcentral.com/1471-2164/15/1134 eel is transferred from FW to SW, the intestinal wall could initiate subsequent intestinal transformations and decreases in thickness, and the anterior intestine be- lead to altered function from FW to SW. came highly vascularized through angiogenesis within + − 1–2 days [8]. Monovalent ions (Na ,Cl)intheim- Results bibed SW are actively absorbed while divalent ions Illumina sequencing and reference gene mapping 2+ 2+ 2− (Ca ,Mg,SO4) are precipitated to decrease Illumina 101 bp paired end sequencing were performed luminal fluid osmolality [5,9]. The composition of on all 25 intestine samples collected from medaka after epithelial transporters is also reorganized extensively. 0 h, 1 h, 3 h, 1d, and 7 d after SW transfer (N = 5) The An upregulation of mucosal Na-K-2Cl cotranspoter sequenced reads ranged from 17.3 to 60.5 million reads (NKCC2/ SLC12A1) remarkably increases the ion ab- with average 37.9 million reads in each sample (Table 1). sorption rate, which is facilitated by the serosal Na-K- The read were mapped against the annotated genome + ATPase and Na -bicarbonate exchanger [10]. The of Medaka. More than 83% of the sequences were rapid and efficient ion absorption mechanism in tele- successfully mapped sequences, indicating the data ost intestine is unique in vertebrates [11]. The SW sets are representative. teleost intestine is an absorptive epithelium that is similar to the thick ascending loop of Henle in mam- Gene ontology analysis malian nephron, and thus was often used as a com- GO terms enriched in the 1 h-after-SW transfer group parative model for the study of kidney tubules, are listed in Table 2. According to biological process, especially in the study of transporter mechanisms and protein phosphorylation, regulation of DNA-dependent cellular regulation in response to volume and salt transcription, cell adhesion, and signal transduction stresses [12]. Several hormones such as cortisol, pro- were highly ranked in GO enrichment. According to lactin, growth hormone, atrial natriuretic peptide, ar- molecular function, protein binding, protein tyrosine ginine vasotocin, guanylin, and vasoactive intestinal kinase activity, protein serine/threonine kinase activity, peptide regulate transepithelial ion transport [13-20]. and actin binding were enriched. According to cellular However, the transcription factors that govern hor- component, significant enrichment in GO terms was mone actions, cell proliferation, apoptosis, angiogen- found in integrin complex and cytoskeleton. esis, transporter metabolism etc., are unclear. Transcriptomic approaches have been used to inves- tigate the dynamics osmoregulatory organ function Transiently upregulated genes of teleosts [21-24]. However, microarray- or pyrose- The intestinal transcription-related genes that are in- quencing-based transcriptomic reads do not provide volved in the SW acclimation were screened from the sufficient depth and coverage for the detection and genes with early transient increase in expression, which quantitation of low expression genes, which may result is defined as genes with significant increases in gene p in a biased discovery towards high expression genes. expression (one-way ANOVA, Tukey; < 0.05) in 1 h In the present study, the objective was to discover the and/or 3 h post-transfer groups compared to those of 0 h, transcription factors that are responsive to the SW 1d, and 7d. The candidates genes were further filtered to re- challenge in medaka intestine. Illumina HiSeq Sequen- move low expression genes (<20 RPM at 1 h or 3 h post- cing was selected to provide a deep coverage of iden- transfer). Genes with significant correlation (Pearson r > 0.8) tified genes as the large number of reads allows in expression with a known osmotic transcription factor quantification of gene expression by mapping to refer- (TSC22D3) among the early transient increase genes ence genome, and is usually sufficient to detect most were selected for further analysis (Additional file 1: expressed gene even at low expression level [25], Table S1). The selected genes were further filtered by which is an ideal choice at a cost-performance per- GO annotation to obtain transcription-related genes spective. Medaka was used owing to the relative com- and 57 genes were further analyzed by real-time PCR. pleteness of the genome data, which can streamline the RNA-seq analysis and guarantee high accuracy and Real-time PCR reliability. Medaka is also a euryhaline species that can To confirm the adequacy of the SW transfer stimulus on survive a direct FW to 50% SW (ca. twice hypertonic the medaka intestine, SLC12A1/NKCC2 and AQP1 were to plasma) transfer [26]. We combined physiological used as reference genes since these transporters are and bioinformatic approaches in the experimental de- well-acknowledged to change in teleost intestine after sign, in which FW medaka was challenged by 50% SW SW challenges [27-29]. The real-time PCR results of transfer and time-dependent changes in intestinal SLC12A1 and AQP1 showed a gradual increase and transcriptomewereanalyzedbyRNA-seq.Wefocused
Load more

Recommended publications
  • A Steroid Receptor Coactivator Stimulator (MCB-613) Attenuates Adverse Remodeling After Myocardial Infarction
    A steroid receptor coactivator stimulator (MCB-613) attenuates adverse remodeling after myocardial infarction Lisa K. Mullanya,1, Aarti D. Rohiraa,1, John P. Leachb,2, Jong H. Kimc,d,2, Tanner O. Monroec, Andrea R. Ortiza, Brittany Storka, M. Waleed Gabere, Poonam Sarkare, Andrew G. Sikoraf, Todd K. Rosengartg, Brian Yorka, Yongcheng Songh, Clifford C. Dacsoa, David M. Lonarda, James F. Martinc,d,3, and Bert W. O’Malleya,3 aDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030; bPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; cDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, TX 77030; dCardiomyocyte Renewal Lab, Texas Heart Institute, Houston, TX 77030; eDepartment of Pediatrics, Baylor College of Medicine, Houston, TX 77030; fDepartment of Otolaryngology-Head & Neck Surgery, Baylor College of Medicine, Houston, TX 77030; gDepartment of Surgery, Baylor College of Medicine, Houston, TX 77030; and hDepartment of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, TX 77030 Contributed by Bert W. O’Malley, September 16, 2020 (sent for review June 23, 2020; reviewed by Chris Glass and Philip W. Shaul) Progressive remodeling of the heart, resulting in cardiomyocyte maintenance of metabolic regulation in diverse organ systems (CM) loss and increased inflammation, fibrosis, and a progressive including the heart (16). Specifically in the heart, recent findings decrease in cardiac function, are hallmarks of myocardial infarction indicate that SRC family members regulate cardiomyocyte func- (MI)-induced heart failure. We show that MCB-613, a potent small tion during early cardiac development (17) and in response to molecule stimulator of steroid receptor coactivators (SRCs) atten- cardiac metabolic stress (18).
    [Show full text]
  • S41467-020-18249-3.Pdf
    ARTICLE https://doi.org/10.1038/s41467-020-18249-3 OPEN Pharmacologically reversible zonation-dependent endothelial cell transcriptomic changes with neurodegenerative disease associations in the aged brain Lei Zhao1,2,17, Zhongqi Li 1,2,17, Joaquim S. L. Vong2,3,17, Xinyi Chen1,2, Hei-Ming Lai1,2,4,5,6, Leo Y. C. Yan1,2, Junzhe Huang1,2, Samuel K. H. Sy1,2,7, Xiaoyu Tian 8, Yu Huang 8, Ho Yin Edwin Chan5,9, Hon-Cheong So6,8, ✉ ✉ Wai-Lung Ng 10, Yamei Tang11, Wei-Jye Lin12,13, Vincent C. T. Mok1,5,6,14,15 &HoKo 1,2,4,5,6,8,14,16 1234567890():,; The molecular signatures of cells in the brain have been revealed in unprecedented detail, yet the ageing-associated genome-wide expression changes that may contribute to neurovas- cular dysfunction in neurodegenerative diseases remain elusive. Here, we report zonation- dependent transcriptomic changes in aged mouse brain endothelial cells (ECs), which pro- minently implicate altered immune/cytokine signaling in ECs of all vascular segments, and functional changes impacting the blood–brain barrier (BBB) and glucose/energy metabolism especially in capillary ECs (capECs). An overrepresentation of Alzheimer disease (AD) GWAS genes is evident among the human orthologs of the differentially expressed genes of aged capECs, while comparative analysis revealed a subset of concordantly downregulated, functionally important genes in human AD brains. Treatment with exenatide, a glucagon-like peptide-1 receptor agonist, strongly reverses aged mouse brain EC transcriptomic changes and BBB leakage, with associated attenuation of microglial priming. We thus revealed tran- scriptomic alterations underlying brain EC ageing that are complex yet pharmacologically reversible.
    [Show full text]
  • Efficient Analysis of Mouse Genome Sequences Reveal Many Nonsense Variants
    Efficient analysis of mouse genome sequences reveal many nonsense variants Sophie Steelanda,b,1, Steven Timmermansa,b,1, Sara Van Ryckeghema,b, Paco Hulpiaua,b, Yvan Saeysa,c, Marc Van Montagud,e,f,2, Roosmarijn E. Vandenbrouckea,b,3, and Claude Liberta,b,2,3 aInflammation Research Center, Flanders Institute for Biotechnology (VIB), 9052 Ghent, Belgium; bDepartment of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium; cDepartment of Internal Medicine, Ghent University, 9052 Ghent, Belgium; dDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium; eDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; and fInternational Plant Biotechnology Outreach, VIB, Ghent, Belgium Contributed by Marc Van Montagu, March 30, 2016 (sent for review December 31, 2015; reviewed by Bruce Beutler, Stefano Bruscoli, Stefan Rose-John, and Klaus Schulze-Osthoff) Genetic polymorphisms in coding genes play an important role alive, archiving them, and distributing mutant strains to in- when using mouse inbred strains as research models. They have terested users (4). been shown to influence research results, explain phenotypical Since Clarence Little showed in the early 20th century that the differences between inbred strains, and increase the amount of principle of inbreeding also applies to mice, several hundred interesting gene variants present in the many available inbred inbred mouse strains have been generated (5). Some of these lines. SPRET/Ei is an inbred strain derived from Mus spretus that strains display specific phenotypes that are the result of a mutant has ∼1% sequence difference with the C57BL/6J reference ge- gene, and in several cases have formed the basis for identifying nome.
    [Show full text]
  • SUPPLEMENTARY NOTE Co-Activation of GR and NFKB
    SUPPLEMENTARY NOTE Co-activation of GR and NFKB alters the repertoire of their binding sites and target genes. Nagesha A.S. Rao1*, Melysia T. McCalman1,*, Panagiotis Moulos2,4, Kees-Jan Francoijs1, 2 2 3 3,5 Aristotelis Chatziioannou , Fragiskos N. Kolisis , Michael N. Alexis , Dimitra J. Mitsiou and 1,5 Hendrik G. Stunnenberg 1Department of Molecular Biology, Radboud University Nijmegen, the Netherlands 2Metabolic Engineering and Bioinformatics Group, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece 3Molecular Endocrinology Programme, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Greece 4These authors contributed equally to this work 5 Corresponding authors E-MAIL: [email protected] ; TEL: +31-24-3610524; FAX: +31-24-3610520 E-MAIL: [email protected] ; TEL: +30-210-7273741; FAX: +30-210-7273677 Running title: Global GR and NFKB crosstalk Keywords: GR, p65, genome-wide, binding sites, crosstalk SUPPLEMENTARY FIGURES/FIGURE LEGENDS AND SUPPLEMENTARY TABLES 1 Rao118042_Supplementary Fig. 1 A Primary transcript Mature mRNA TNF/DMSO TNF/DMSO 8 12 r=0.74, p< 0.001 r=0.61, p< 0.001 ) 2 ) 10 2 6 8 4 6 4 2 2 0 Fold change (mRNA) (log Fold change (primRNA) (log 0 −2 −2 −2 0 2 4 −2 0 2 4 Fold change (RNAPII) (log2) Fold change (RNAPII) (log2) B chr5: chrX: 56 _ 104 _ DMSO DMSO 1 _ 1 _ 56 _ 104 _ TA TA 1 _ 1 _ 56 _ 104 _ TNF TNF Cluster 1 1 _ Cluster 2 1 _ 56 _ 104 _ TA+TNF TA+TNF 1 _ 1 _ CCNB1 TSC22D3 chr20: chr17: 25 _ 33 _ DMSO DMSO 1 _ 1 _ 25 _ 33 _ TA TA 1 _ 1 _ 25 _ 33 _ TNF TNF Cluster 3 1 _ Cluster 4 1 _ 25 _ 33 _ TA+TNF TA+TNF 1 _ 1 _ GPCPD1 CCL2 chr6: chr22: 77 _ 35 _ DMSO DMSO 1 _ 77 _ 1 _ 35 _ TA TA 1 _ 1 _ 77 _ 35 _ TNF Cluster 5 Cluster 6 TNF 1 _ 1 _ 77 _ 35 _ TA+TNF TA+TNF 1 _ 1 _ TNFAIP3 DGCR6 2 Supplementary Figure 1.
    [Show full text]
  • Full Text (PDF)
    The Journal of Immunology Enhancer Turnover Is Associated with a Divergent Transcriptional Response to Glucocorticoid in Mouse and Human Macrophages Alasdair W. Jubb,*,†,1 Robert S. Young,*,1 David A. Hume,† and Wendy A. Bickmore* Phenotypic differences between individuals and species are controlled in part through differences in expression of a relatively con- served set of genes. Genes expressed in the immune system are subject to especially powerful selection. We have investigated the evolution of both gene expression and candidate enhancers in human and mouse macrophages exposed to glucocorticoid (GC), a regulator of innate immunity and an important therapeutic agent. Our analyses revealed a very limited overlap in the repertoire of genes responsive to GC in human and mouse macrophages. Peaks of inducible binding of the GC receptor (GR) detected by chro- matin immunoprecipitation-Seq correlated with induction, but not repression, of target genes in both species, occurred at distal regulatory sites not promoters, and were strongly enriched for the consensus GR-binding motif. Turnover of GR binding between mice and humans was associated with gain and loss of the motif. There was no detectable signal of positive selection at species- specific GR binding sites, but clear evidence of purifying selection at the small number of conserved sites. We conclude that enhancer divergence underlies the difference in transcriptional activation after GC treatment between mouse and human macrophages. Only the shared inducible loci show evidence of selection, and therefore these loci may be important for the subset of responses to GC that is shared between species. The Journal of Immunology, 2016, 196: 813–822.
    [Show full text]
  • Insights Into Functional Connectivity in Mammalian Signal Transduction Pathways by Pairwise
    bioRxiv preprint doi: https://doi.org/10.1101/2019.12.30.891200; this version posted December 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Insights into Functional Connectivity in Mammalian Signal Transduction Pathways by Pairwise Comparison of Protein Interaction Partners of Critical Signaling Hubs Chilakamarti V. Ramana * Department of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03766, USA *Correspondence should be addressed: Chilakamarti V .Ramana, Telephone. (603)-738-2507, E-mail: [email protected] ORCID ID: /0000-0002-5153-8252 1 bioRxiv preprint doi: https://doi.org/10.1101/2019.12.30.891200; this version posted December 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract Growth factors and cytokines activate signal transduction pathways and regulate gene expression in eukaryotes. Intracellular domains of activated receptors recruit several protein kinases as well as transcription factors that serve as platforms or hubs for the assembly of multi-protein complexes. The signaling hubs involved in a related biologic function often share common interaction proteins and target genes. This functional connectivity suggests that a pairwise comparison of protein interaction partners of signaling hubs and network analysis of common partners and their expression analysis might lead to the identification of critical nodes in cellular signaling.
    [Show full text]
  • Glucocorticoid Receptor and Klf4 Co-Regulate Anti-Inflammatory Genes in Keratinocytes
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Digital.CSIC Glucocorticoid receptor and Klf4 co-regulate anti-inflammatory genes in keratinocytes Lisa M. Sevilla1, Víctor Latorre1,2, Elena Carceller1, Julia Boix1, Daniel Vodák3, Ian Geoffrey Mills4, 5, 6, and Paloma Pérez1 1 Instituto de Biomedicina de Valencia-Consejo Superior de Investigaciones Científicas (IBV- CSIC), Jaime Roig 11, E-46010 Valencia, Spain. 2 Faculty of Human and Medical Sciences, The University of Manchester, Manchester UK 3 Bioinformatics Core Facility, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Norway 4 Prostate Cancer Research Group, Centre for Molecular Medicine (Norway), University of Oslo and Oslo University Hospitals, Oslo, Norway 5 Department of Cancer Prevention, Oslo University Hospitals, Oslo, Norway. 6 Department of Urology, Oslo University Hospitals, Oslo, Norway. Corresponding autor and person to whom reprint requests should be addressed: Paloma Pérez, PhD Instituto de Biomedicina de Valencia-Consejo Superior de Investigaciones Científicas (IBV- CSIC) Jaime Roig 11, E-46010, Valencia, Spain Phone: +34-96-339-1766 Fax: +34-96-369-0800 e-mail: [email protected] Grants and fellowships supporting the writing of paper: SAF2011-28115 of the Ministerio de Economía y Competitividad from the Spanish government. EC and JB are recipients of FPU and FPI fellowships of the Spanish Ministery, respectively Disclosure Statement: The authors have nothing to disclose. Abstract The glucocorticoid (GC) receptor (GR) and Kruppel-like factor Klf4 are transcription factors that play major roles in the skin homeostasis by regulating the expression of overlapping gene subsets.
    [Show full text]
  • GILZ-Dependent Modulation of Mtorc1 Regulates Spermatogonial Maintenance Hue M
    © 2018. Published by The Company of Biologists Ltd | Development (2018) 145, dev165324. doi:10.1242/dev.165324 STEM CELLS AND REGENERATION RESEARCH ARTICLE GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance Hue M. La1,2,*, Ai-Leen Chan1,2,*, Julien M. D. Legrand1,2, Fernando J. Rossello1,2, Christina G. Gangemi1,2, Antonella Papa3, Qiang Cheng4, Eric F. Morand4 and Robin M. Hobbs1,2,‡ ABSTRACT prospermatogonia) upon migration to the basement membrane of Male fertility is dependent on spermatogonial stem cells (SSCs) that the seminiferous cords. The undifferentiated population contains self-renew and produce differentiating germ cells. Growth factors isolated spermatogonia (A-single or As) plus chains of cells produced within the testis are essential for SSC maintenance interconnected by cytoplasmic bridges. Two-cell chains are but intrinsic factors that dictate the SSC response to these stimuli known as A-paired (Apr) whereas chains of four or more cells are poorly characterised. Here, we have studied the role of GILZ, are known as A-aligned (Aal). Lineage-tracing studies demonstrate α a TSC22D family protein and spermatogenesis regulator, in that SSCs are marked by GFR 1 and typically As and Apr,whereas spermatogonial function and signalling. Although broadly expressed most undifferentiated cells, particularly Aal, are committed in the germline, GILZ was prominent in undifferentiated spermatogonia progenitors and marked by NGN3 (Hara et al., 2014; Nakagawa + and Gilz deletion in adults resulted in exhaustion of the GFRα1+ et al., 2010). NGN3 Aal may revert to SSCs through chain SSC-containing population and germline degeneration. GILZ loss fragmentation, particularly upon tissue damage (Nakagawa et al., γ was associated with mTORC1 activation, suggesting enhanced 2010).
    [Show full text]
  • TSC22D3 (NM 001015881) Human Tagged ORF Clone Product Data
    OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RG214246 TSC22D3 (NM_001015881) Human Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: TSC22D3 (NM_001015881) Human Tagged ORF Clone Tag: TurboGFP Symbol: TSC22D3 Synonyms: DIP; DSIPI; GILZ; TSC-22R Vector: pCMV6-AC-GFP (PS100010) E. coli Selection: Ampicillin (100 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RG214246 representing NM_001015881 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACCGAAATGTATCAGACCCCCATGGAGGTGGCGGTCTACCAGCTGCACAATTTCTCCATCTCCT TCTTCTCTTCTCTGCTTGGAGGGGATGTGGTTTCCGTTAAGCTGGACAACAGTGCCTCCGGAGCCAGCGT GGTGGCCATAGACAACAAGATCGAACAGGCCATGGATCTGGTGAAGAATCATCTGATGTATGCTGTGAGA GAGGAGGTGGAGATCCTGAAGGAGCAGATCCGAGAGCTGGTGGAGAAGAACTCCCAGCTAGAGCGTGAGA ACACCCTGTTGAAGACCCTGGCAAGCCCAGAGCAGCTGGAGAAGTTCCAGTCCTGTCTGAGCCCTGAAGA GCCAGCTCCCGAATCCCCACAAGTGCCCGAGGCCCCTGGTGGTTCTGCGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence: >RG214246 representing NM_001015881 Red=Cloning site Green=Tags(s) MNTEMYQTPMEVAVYQLHNFSISFFSSLLGGDVVSVKLDNSASGASVVAIDNKIEQAMDLVKNHLMYAVR EEVEILKEQIRELVEKNSQLERENTLLKTLASPEQLEKFQSCLSPEEPAPESPQVPEAPGGSAV TRTRPLE - GFP Tag - V Restriction Sites: SgfI-MluI This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies,
    [Show full text]
  • Arthritis Rheumatoid Disease Severity in Patients with B, Is Associated
    A Truncated Variant of ASCC1, a Novel Inhibitor of NF- κB, Is Associated with Disease Severity in Patients with Rheumatoid Arthritis This information is current as of September 25, 2021. Silvia Torices, Lorena Alvarez-Rodríguez, Lara Grande, Ignacio Varela, Pedro Muñoz, Dora Pascual, Alejandro Balsa, Marcos López-Hoyos, Víctor Martinez-Taboada and Jose L. Fernández-Luna J Immunol 2015; 195:5415-5420; Prepublished online 26 Downloaded from October 2015; doi: 10.4049/jimmunol.1501532 http://www.jimmunol.org/content/195/11/5415 http://www.jimmunol.org/ References This article cites 28 articles, 13 of which you can access for free at: http://www.jimmunol.org/content/195/11/5415.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision by guest on September 25, 2021 • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2015 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology A Truncated Variant of ASCC1, a Novel Inhibitor of NF-kB, Is Associated with Disease Severity in Patients with Rheumatoid Arthritis Silvia Torices,*,† Lorena Alvarez-Rodrı´guez,* Lara Grande,† Ignacio Varela,‡ Pedro Mun˜oz,x Dora Pascual,{ Alejandro Balsa,{ Marcos Lo´pez-Hoyos,‖ Vı´ctor Martinez-Taboada,*,# and Jose L.
    [Show full text]
  • Implicating the Role of GILZ in Glucocorticoid Modulation of T-Cell Activation
    MINI REVIEW published: 07 August 2019 doi: 10.3389/fimmu.2019.01823 Implicating the Role of GILZ in Glucocorticoid Modulation of T-Cell Activation Lorenza Cannarile, Domenico V. Delfino, Sabrina Adorisio, Carlo Riccardi and Emira Ayroldi* Section of Pharmacology, Department of Medicine, Medical School, University of Perugia, Perugia, Italy Glucocorticoid-induced leucine zipper (GILZ) is a protein with multiple biological roles that is upregulated by glucocorticoids (GCs) in both immune and non-immune cells. Importantly, GCs are immunosuppressive primarily due to their regulation of cell signaling pathways that are crucial for immune system activity. GILZ, which is transcriptionally induced by the glucocorticoid receptor (GR), mediates part of these immunosuppressive, and anti-inflammatory effects, thereby controlling immune cell proliferation, survival, and differentiation. The primary immune cells targeted by the immunosuppressive activity of GCs are T cells. Importantly, the effects of GCs on T cells are partially Edited by: Massimo Gadina, mediated by GILZ. In fact, GILZ regulates T-cell activation, and differentiation by binding National Institute of Arthritis and and inhibiting factors essential for T-cell function. For example, GILZ associates with Musculoskeletal and Skin Diseases nuclear factor-κB (NF-κB), c-Fos, and c-Jun and inhibits NF-κB-, and AP-1-dependent (NIAMS), United States transcription. GILZ also binds Raf and Ras, inhibits activation of Ras/Raf downstream Reviewed by: Marc Pallardy, targets, including mitogen-activated protein kinase 1 (MAPK1). In addition GILZ inhibits Université Paris-Sud, France forkhead box O3 (FoxO3) without physical interaction. GILZ also promotes the activity of Géraldine Schlecht-Louf, Université Paris-Sud, France regulatory T cells (Tregs) by activating transforming growth factor-β (TGF-β) signaling.
    [Show full text]
  • Single Cell Analysis Reveals X Chromosome Upregulation Is Not
    bioRxiv preprint doi: https://doi.org/10.1101/2021.07.18.452817; this version posted July 19, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Single cell analysis reveals X chromosome upregulation is not global and 2 primarily belongs to ancestral genes in pre-gastrulation embryos 3 Naik C H, Chandel D, and Gayen S* 4 Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, 5 Bangalore-560012, India. 6 *Correspondence: [email protected] 7 8 Abstract 9 Recent studies have provided substantial evidence supporting Ohno's hypothesis that 10 upregulation of active X chromosome genes balances the dosage of X-linked gene expression 11 relative to autosomal genes. However, the dynamics of X-chromosome upregulation (XCU) 12 during early development remain poorly Pre-gastrulation embryos E6.50 13 understood. Here, we have profiled the E6.25 E5.5 14 dynamics of XCU in different lineages of 15 female pre-gastrulation mouse embryos at 16 single cell level through allele-specific single 17 cell RNA-seq analysis. We found dynamic 18 XCU upon initiation of random X- Epiblast cells 19 chromosome inactivation (XCI) in epiblast Onset of Random X-inactivation 20 cells and cells of extraembryonic lineages, 21 which undergo imprinted XCI, also harbored 22 upregulated active-X chromosome. On the 23 other hand, the extent of XCU remains 24 controversial till date.
    [Show full text]