Repository of the Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association http://edoc.mdc-berlin.de/14998 DULIP: A dual luminescence-based co-immunoprecipitation assay for interactome mapping in mammalian cells Trepte, P., Buntru, A., Klockmeier, K., Willmore, L., Arumughan, A., Secker, C., Zenkner, M., Brusendorf, L., Rau, K., Redel, A., Wanker, E.E. NOTICE: this is the author’s version of a work that was accepted for publication in the Journal of Molecular Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in: Journal of Molecular Biology 2015 MMM DD ; 427(21): 3375-3388 doi: 10.1016/j.jmb.2015.08.003 Publisher: Elsevier © 2015, Elsevier. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA. DULIP: A DUAL LUMINESCENCE-BASED CO-IMMUNOPRECIPITATION ASSAY FOR INTERACTOME MAPPING IN MAMMALIAN CELLS Philipp Treptea, Alexander Buntrua#, Konrad Klockmeiera#, Lindsay Willmorea, Anup Arumughana, Christopher Seckera, Martina Zenknera, Lydia Brusendorfa, Kirstin Raua, Alexandra Redela and Erich E Wankera* a Neuroproteomics, Max Delbrueck Center for Molecular Medicine, Robert-Roessle- Straße 10, 13125 Berlin, Germany # Contributed equally * Corresponding author, E-mail address:
[email protected], Telephone: +49- 30-9406-2157, Fax: +49-30-9406-2552 ABSTRACT Mapping of protein-protein interactions (PPIs) is critical for understanding protein function and complex biological processes.