Anti-Crkii Antibody (ARG57859)

Product datasheet [email protected]

ARG57859 Package: 50 μl anti-CrkII antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes CrkII

Tested Reactivity Hu

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name CrkII

Antigen Species Human

Immunogen Recombinant protein of Human CrkII.

Conjugation Un-conjugated

Alternate Names CRKII; p38; Proto-oncogene c-Crk; Adapter molecule crk

Application Instructions

Application table Application Dilution

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control HepG2

Calculated Mw 34 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Gene Symbol CRK

Gene Full Name v-crk avian sarcoma virus CT10 oncogene homolog

Background This gene encodes a member of an adapter protein family that binds to several tyrosine- phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

Function The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk- I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac- dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. [UniProt]

Cellular Localization Cytoplasm, Cell membrane. [UniProt]

Images

ARG57859 anti-CrkII antibody WB image

Western blot: 25 µg of HepG2 cell lysate stained with ARG57859 anti- CrkII antibody.