The Extracellular Matrix for Bone Regeneration
This chapter is based on human mesenchymal stromal cells in adhesion to cell-derived 5 extracellular matrix and titanium: a comparative kinome profi le analysis Marta Baroncelli1^, Gwenny M. Fuhler2^, Jeroen van de Peppel1, Willian F. Zambuzzi3, Johannes P.T.M van Leeuwen1, Bram C.J van der Eerden1°, Maikel P. Peppelenbosch2° 1Department of Internal Medicine, Erasmus MC. University Medical Center Rotterdam, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands 2Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands 3Laboratorio de Bioensaios e Dinâmica Celular, Dep.to de Quimica e Bioquimica, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Campus Botucatu, São Paulo, SP Brazil. ^, °: both authors contributed equally to this work Submitted Chapter 5 ABSTRACT The extracellular matrix (ECM) is an essential component of tissue architecture that physically supports cells and actively influences stem cell behaviour, by modulating kinase-mediated signaling cascades that are the key regulators of signal transduc- tion. Cell-derived ECMs have recently emerged in the context of bone regeneration, as they reproduce physiological tissue-architecture and ameliorate the promising properties of mesenchymal stromal cells (MSCs). Titanium scaffolds show good mechanical properties and porosity to facilitate cell adhesion, and thus have been routinely used for bone tissue engineering applications. The aim of this study was to analyze the kinomic signature of human MSCs in adhesion to an osteoblast-derived ECM that we have previously shown to be osteopromotive, and to compare it to MSCs on titanium. PamChip kinase array analysis revealed 63 phosphorylated peptides on ECM and 59 on titanium, with MSCs on ECM exhibiting significant higher levels of kinase activity than those on titanium.
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