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Provided for Non-Commercial Research and Educational Use Only. Not for Reproduction Or Distribution Or Commercial Use Provided for non-commercial research and educational use only. Not for reproduction or distribution or commercial use. This article was originally published by IWA Publishing. IWA Publishing recognizes the retention of the right by the author(s) to photocopy or make single electronic copies of the paper for their own personal use, including for their own classroom use, or the personal use of colleagues, provided the copies are not offered for sale and are not distributed in a systematic way outside of their employing institution. Please note that you are not permitted to post the IWA Publishing PDF version of your paper on your own website or your institution’s website or repository. Please direct any queries regarding use or permissions to [email protected] 1406 © IWA Publishing 2011 Water Science & Technology | 64.7 | 2011 Comparative 16S rRNA gene surveys of granular sludge from three upflow anaerobic bioreactors treating purified terephthalic acid (PTA) wastewater S. D. Perkins, N. B. Scalfone and L. T. Angenent ABSTRACT The microbial communities from three upflow anaerobic bioreactors treating purified terephthalic S. D. Perkins Department of Energy, acid (PTA) wastewater were characterized with 16S ribosomal RNA gene sequencing surveys. Environment and Chemical Engineering, Washington University in St. Louis, Universal bacterial and archaeal primers were used to compare the bioreactor communities to each St. Louis, MO 63130, USA other. A total of 1,733 bacterial sequences and 383 archaeal sequences were characterized. The high Syntrophus Pelotomaculum fi N. B. Scalfone number of spp. and spp. found within these reactors indicates ef cient L. T. Angenent (corresponding author) removal of benzoate and terephthalate. Under anaerobic conditions benzoate can be degraded Biological and Environmental Engineering, Cornell University, through syntrophic associations between these bacteria and hydrogen-scavenging microbes, such as 214 Riley-Robb Hall, Ithaca, NY 14853, Desulfovibrio spp. and hydrogenotrophic methanogens, which remove H2 to force the USA E-mail: [email protected] thermodynamically unfavourable reactions to take place. The authors did not observe a relatively high percentage of hydrogenotrophic methanogens with the archaeal gene survey because of a high acetate flux (acetate is a main component in PTA wastewater and is the main degradation product of terephthalate/benzoate fermentation), and because of the presence of Desulfovibrio spp. (a sulfate reducer that scavenges hydrogen). The high acetate flux also explains the high percentage of acetoclastic methanogens from the genus Methanosaeta among the archaeal sequences. A group of uncultured bacteria (OD1) may be involved in the degradation of p-toluate (4-methyl benzoate), which is a component of PTA wastewater. Key words | anaerobic digestion, methanogens, p-toluate, PTA wastewater, 16S rRNA gene survey, terephthalate INTRODUCTION Purified terephthalic (p-phthalic or 1,4-benzenedicar- which can be accomplished in upflow bioreactors, such as boxylic) acid (PTA) wastewater has been successfully upflow anaerobic sludge blanket (UASB) reactors and its off- treated in anaerobic digesters at chemical companies that shoots, with granular biomass to sustain long residence produce polyethylene terephthalate to manufacture, for times (Kleerebezem et al. ). But even after a sustainable example, plastic bottles for the beverage industry (Qiu period of terephthalate degradation, high concentrations of et al. ; Kleerebezem et al. b, ). This wastewater acetate and benzoate (itself the product of terephthalate contains terephthalate, acetate, benzoate, and p-toluate degradation) can disrupt this (Kleerebezem et al. b). (4-methyl-benzoate) among other chemicals (Zhang et al. These authors also discussed that the same bacterium may ). Even though these chemicals are not inhibiting the be degrading both terephthalate and benzoate. p-Toluate methanogenic biomass at the concentrations observed in can be removed, albeit at even lower rates than terephthal- PTA wastewater, terephthalate and p-toluate are not ate in methanogenic environments, which is why some always degraded in anaerobic upflow bioreactors, while have suggested the use of aerobic post-treatment to remove the biodegradation of acetate and benzoate is relatively p-toluate (Zhang et al. ). In high-rate anaerobic bio- easy (Kleerebezem et al. ). Terephthalate under anaero- reactors, such as UASB reactors or expanded sludge bed bic conditions is degraded at low rates after long lag periods, reactors (EGSBs), the high concentration of granular doi: 10.2166/wst.2011.552 1407 S. D. Perkins et al. | Comparative 16s rRNA gene surveys of granular sludge from anaerobic bioreactors Water Science & Technology | 64.7 | 2011 biomass and long cell residence times may overcome the consists of a tank with an internal diameter of 13 m and slow removal rates of p-toluate. Here, we surveyed three aheightof15m(∼2,000 m3 wet volume). This reactor granular samples from upflow anaerobic bioreactors treating was started ∼4 years prior to sampling with a sCOD PTA wastewater to characterize their microbial commu- removal efficiency of ∼60%. This relatively low sCOD nities with nonculturing methods. We used Sanger removal efficiency indicates that the most difficult to be sequencing to sequence near full-length sequences of 16S removed chemicals, such as p-toluate, were not degraded ribosomal RNA (rRNA) genes that were amplified with bac- in the anaerobic system. terial or archaeal specific PCR primers. Our goal was to compare the communities of the upflow bioreactors with DNA extraction and PCR amplification different operating conditions and performance, especially in regard to the removal of p-toluate. Received samples were immediately stored at –80 W C until further processing. Granules were crushed using a sterile spa- tula (Corning 3003, Corning, NY) before 0.5 ml of sample METHODS was submitted to a bead-beating and phenol:chloroform extraction protocol. The 16S rRNA genes from bacteria Granular samples were amplified with a 30-cycle PCR (initial 2 min denaturing step at 94 W C, followed by 30 cycles at 92 W C for 30 s 45 W C We surveyed granular samples from two lab-scale UASB for 90 s, 72 W C for 90 s, and a final 72 W C extension for reactors (samples U1 and U2, received 17 June 2008); 15 min). The 50-μl solution contained 1.25 units of GoTaq and from a full-scale expanded granular sludge bed (Promega Corp, Madison, WI), 0.4 pmol/μl forward and (EGSB)-Biobed reactor (E1, received 5 August 2008). The reverse primers (8F-50 AGAGTTTGATCCTGGCTCAG-30; UASB reactors had an internal diameter of 0.10 m with a 1391R-50 GACGGGCGGTGWGTRCA-30), 0.5 mmol/L height of 1.2 m (∼9 L wet volume). U1 was operated with MgCl2, 0.2 mmol/L dNTPs, 0.8 mg/mL BSA, and 2-μlof an upflow velocity of 1.9 m/h, a temperature of 38 W C, template. Archaeal 16S rRNA genes were amplified with a and an average pH of 7.2 during a three-month period 30-cycle PCR (initial 2 min denaturing step at 94 W C, followed before sampling. This reactor had been operating for a by 30 cycles at 94 W C for 60 s, 55 W C for 60 s, 72 W C for 60 s, and period of 15 months after inoculation with granular a final 72 W C extension for 10 min). A similar PCR solution sludge from upflow anaerobic bioreactors located at a brew- was used with different primers: 21F-50 AAGGAGGTGATC ery (70% v/v) and a papermill (8%), and from U2 (22%). A CAGCC-30; 1492R-50 GGTTACCTTGTTACGACTT-30. Con- synthetic PTA wastewater was fed to U1 at a loading rate of trol positive and negative reactions were included with 42.6 g COD/L/day, a hydraulic retention time (HRT) of 14 each reaction set. h, and a soluble COD (sCOD) removal efficiency of 98.7%. U2 was operated with an upflow velocity of 1.7 m/h, a Cloning and sequence analysis temperature of 38 W C, and an average pH of 7.2 during a three-month period. This reactor had been operating for PCR products were cleaned and concentrated (Montage a period of 21 months after inoculation with granular PCR cleaning kit, UFC7PCR50, Millipore, Billerica, MA) sludge from upflow anaerobic bioreactors at two breweries and sent to the Genome Sequencing Center, Washington and a petrochemical company. A synthetic PTA wastewater University School of Medicine in St. Louis, for cloning, puri- was fed to U2 at a loading rate of 27.4 g COD/L/day, a fication, and Sanger sequencing on an ABI 3730 capillary HRT of 12 h, and a sCOD removal efficiency of 99.0%. sequencer (Applied Biosystems, Foster City, CA). Three The difference between U1 and U2 was the composition independent PCR amplicon materials were sequenced per of the PTA wastewater, which mimicked two different pro- sample for bacteria and one for Archaea. 16S rRNA gene cessing plants. The high sCOD removal efficiencies show sequences were edited and assembled into consensus that almost all chemicals (including p-toluate) in this type sequences using PHRED and PHRAP aided by XplorSeq of wastewater were completely removed under methano- (Frank ). Bases with a PHRAP quality score <20 were genic conditions and converted to biogas, and that the trimmed from the dataset before being aligned using the intermediate volatile fatty acids were maintained at very NAST online tool (DeSantis et al. b). Chimeras were low concentrations in the effluent. The full-scale E1 detected and removed using Bellerophon (Huber et al. system is located at a petrochemical industrial site and ). Nonchimeric sequences were compared to the 1408 S. D. Perkins et al. | Comparative 16s rRNA gene surveys of granular sludge from anaerobic bioreactors Water Science & Technology | 64.7 | 2011 greengenes public database and imported into ARB Nucleotide sequence accession numbers (Ludwig et al. ). A pair-wise identity of 97% or greater was used to bin sequences into ‘species’-level operational Near full-length bacterial 16S rRNA gene sequences were taxonomic units (OTUs).
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