Human CCL13/MCP-4 Antibody
Monoclonal Mouse IgG1 Clone # 73506 Catalog Number: MAB327
DESCRIPTION Species Reactivity Human
Specificity Detects human CCL13/MCP4 in ELISAs. In sandwich immunoassays, less than 1% crossreactivity with recombinant human (rh) CCL2 is observed and no significant crossreactivity or interference with rhCCL5, 7, 8, 11, 24, recombinant mouse CCL2, 5, 7, 11 or 12 is observed.
Source Monoclonal Mouse IgG1 Clone # 73506 Purification Protein A or G purified from ascites
Immunogen E. coliderived recombinant human CCL13/MCP4 Gln24Thr98 Accession # Q99616.1
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method. Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Intracellular Staining by Flow Cytometry 2.5 µg/106 cells A549 human lung carcinoma cell line treated with recombinant human IL1β, fixed with paraformaldehyde and permeabilized with saponin Human CCL13/MCP4 Sandwich Immunoassay Reagent ELISA Capture 28 µg/mL Human CCL13/MCP4 Antibody (Catalog # MAB327) ELISA Detection 0.10.4 µg/mL Human CCL13/MCP4 Biotinylated Antibody (Catalog # BAF327) Standard Recombinant Human CCL13/MCP4 (Catalog # 327P4) CyTOFready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Neutralization Measured by its ability to neutralize CCL13/MCP4induced chemotaxis in the BaF3 mouse proB cell line
transfected with human CCR2A. The Neutralization Dose (ND50) is typically 1030 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP4.
DATA
Neutralization Chemotaxis Induced by CCL13/MCP4 and Neutral ization by Human CCL13/ MCP4 Antibody. Recombinant Human CCL13/ MCP4 (Catalog # 327P4) chemoattracts the BaF3 mouse proB cell line transfected with human CCR2A in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse AntiHuman CCL13/ MCP4 Monoclonal Antibody
(Catalog # MAB327). The ND50 is typically 1030 µg/mL.
PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at 20 to 70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 20 to 70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, 20 to 70 °C under sterile conditions after reconstitution.
Rev. 2/7/2018 Page 1 of 2
Human CCL13/MCP-4 Antibody
Monoclonal Mouse IgG1 Clone # 73506 Catalog Number: MAB327
BACKGROUND CCL13, also known as Monocyte Chemoattractant Protein4 (MCP4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential Nglycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP1, 3 and Eotaxin, exhibiting approximately 6566% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR2 and CCR3.
Rev. 2/7/2018 Page 2 of 2