Western Michigan University ScholarWorks at WMU

Master's Theses Graduate College

8-1972

Spectral Analysis of Olfactory System Electrical Activity: Effects of and Sodium

John Ludwig Orr

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Recommended Citation Orr, John Ludwig, "Spectral Analysis of Olfactory System Electrical Activity: Effects of Ketamine and Sodium Pentobarbital" (1972). Master's Theses. 2815. https://scholarworks.wmich.edu/masters_theses/2815

This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. SPECTRAL ANALYSIS OF OLFACTORY SYSTEM ELECTRICAL ACTIVITY EFFECTS OF KETAMINE AND SODIUM PENTOBARBITAL

by

John Ludwig Orr

A Thesis Submitted to the Faculty of the Graduate College in partial fulfillment of the Degree of Master of Arts

Western Michigan University Kalamazoo, Michigan August 1972

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ACKNOWLEDGEMENTS

In the course of preparing this thesis I have benefited from

advice and discussions with Drs. Fred Gault, Ronald Hutchinson and

Arthur Snapper- My thanks go to them and especially the staff at

the Western Michigan University Computer Center who gave patiently

of their time. Of course, I assume the sole responsibility for what

is written here.

John Ludwig Orr

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. INFORMATION TO USERS

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Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. MASTERS THESIS M-4247

ORR, John Ludwig SPECTRAL ANALYSIS OF OLFACTORY SYSTEM ELECTRICAL ACTIVITY: EFFECTS OF KETAMINE AND SODIUM PENTOBARBITAL.

Western Michigan University, M.A., 1972 Physiology

University Microfilms, A XEROX Company, Ann Arbor, Michigan

THIS DISSERTATION HAS BEEN MICROFILMED EXACTLY AS RECEIVED.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PLEASE NOTE:

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indistinct print.

Filmed as received.

University Microfilms, A Xerox Education Company

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. TABLE OF CONTENTS

CHAPTER PAGE

I INTRODUCTION...... 1

The Purpose...... 1

Advantages of Digital Analysis ...... 2

Window Closing ...... 3

Stationarity ...... 3

Stability and Fidelity ...... 4

II METHODS AND MATERIALS ...... 5

Electrodes ...... 5

Implantation ...... 5

Recording...... 6

Ketamine...... 7

Barbiturate...... 7

Digital Spectral Analysis...... 7

III RESULTS...... 10

IV DISCUSSION...... 40

V REFERENCES...... 47

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. INTRODUCTION

Time domain displays of the electrical activity of the olfactory

system are similar across a wide range of species (Adrian, 1950).

The analysis of component frequencies in olfactory electrical activity

by analog methods led Hughes and Hendrix (1967) to propose that the

frequency components encode information concerning the nature of an

olfactory stimulus. The purpose of this study was to implement

digital frequency analysis techniques on a large digital computer

to obtain the frequency components in olfactory system electrical

activity and evaluate a potentially useful class of for

use in recording electrical activity in acute preparations.

Olfactory system electrical activity is intimately related to the

activity of the midbrain reticular formation and is very sensitive to

the effects of anesthetics. Electrical stimulation of the midbrain

reticular formation increases the amplitude of olfactory spindle

bursts (Pagano, 1966), and , which depress the reticular

system (Sharpless, 1970) , reduce the frequency of olfactory spindle

bursts (Hemandez-Peon, Lavin, Alcocer-Cuaron, & Marcelin, 1960;

Gault & Coustan, 1965). Ether, an frequently used in

setting up acute preparations, disrupts the electrical activity of

the olfactory bulb for up to six hours (Gault & Coustan, 1965). Thus

for olfactory research with acute preparations, an anesthetic which

did not depress the reticular system might prove useful.

1

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 2

"Ketamine has a stronger depressant effect on the diffuse

thalamic projection system than on the midbrain reticular formation"

(Miyasaka & Domino, 1968, p. 570). This conclusion, based on time

domain displays, suggested that Ketamine would be a useful anesthetic

for electrical recording in acute preparations- "Disassociative

anesthesia" is the term introduced (Domino, Chodoff, & Corssen, 1965)

to describe the state induced by Ketamine.

To evaluate Ketamine as an anesthetic for olfactory acute

preparations, rats with chronically implanted olfactory system

electrodes were used. This chronic preparation allows the recording

of electrical activity before and after drug administration without

the confounding effects of other drugs or surgical trauma. Sodium

Pentobarbital, a , was included in the evaluation for

comparison.

The present research is closely related to that of Pagano (1966).

Pagano's measure of olfactory system electrical activity was the

integrated output of a bandpass filter centered at 40 Hz. The power

spectrum, the dependent variable in the present research, may be

considered the distribution of the integrated electrical activity

by frequency.

The analysis of electrical activity into components may be

performed with either analog or digital apparatus. At the present

time, analog methods do not have all the advantages of digital methods

implemented on a large digital computer. For example, multivariate

spectra have been computed by digital methods and plots of phase and

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. coherence spectra used to describe the relationships between time

series recorded from different electrodes (Walter & Adey, 1965).

A major advantage of digital analysis is that the analysis bandwidth

may be easily varied. This leads to the routine practice of

conducting the analysis of a given time series at several bandwidths.

Spectra obtained with the different bandwidths are then compared to

judge the quality of the digital analysis for a given number of data

points. This process is called window closing and allows after the

fact selection of the most suitable bandwidth for a given record

(Jenkins & Watts, 1968, pp. 280-281).

Spectral analysis is the term used to refer to adaptations of

Fourier analysis to the analysis of nondeterminate stationary time

series. Digital cross-spectral analysis was first applied to the

electrical activity of the brain in studies of learning by Adey,

Walter, and Hendrix (1961). Since that time a number of reports

involving spectral analysis of brain electrical activity have

appeared, including Walter (1963), Adey (1965), Elazar and Adey (1967)

and Adey (1969).

Stationarity is a concept important to the evaluation of the

appropriateness of spectral analysis because a stationary time series

is completely described by the lower moments of its probability

distribution (Jenkins & Watts, 1968, p. 3). The lower moments

involved in the description of a stationary time series are the mean,

standard deviation, autocovariance, and the power spectrum, which is

the Fourier transform of the autocovariance (Jenkins & Watts, 1968, p.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. A stationary time series is one which exists in a state of statistical

equilibrium with no trends (Jenkins & Watts, 1968, p. 4); in other

words, the series is independent of clock time (Jenkins & Watts, 1968,

p. 147). Even in cases where the empirical time series is non-

stationary, spectral methods for stationary series are an appropriate

starting point because nonstationarity in the mean will appear as a

low frequency peak in the spectrum (Jenkins & Watts, 1968, p. 8).

A useful test for self-stationarity is the examination of more

than one spectra obtained in a given experimental condition. If the

variability is small, the process generating the spectra is self-

stationary (Bendat & Piersol, 1966).

Spectral analysis has the property that the product of the

bandwidth of the digital filters and the variance of the spectrum

obtained is a constant (Jenkins & Watts, 1968, p. 257). This means

that to obtain a high degree of spectral stability for a given length

of record, one should use wide bandwidth digital filters. Fidelity,

however, depends upon having digital filters on the same order of

magnitude of bandwidth as the narrowest detail of interest in the

spectrum (Jenkins & Watts, 1968, p. 279). The challenge of conducting

an analysis which is stable enough to demonstrate spectral changes

between experimental conditions and yet allows the display of as much

spectral detail as possible is met by window closing.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. METHODS AND MATERIALS

Four male Sprague-Dawley albino rats from the Upjohn Company

colony were implanted with coaxial bipolar electrodes under Sodium

Pentobarbital anesthesia in clean but not aseptic conditions

Atropine sulfate was administered to reduce respiratory system

secretions. The electrodes were constructed from 26 gauge #303

stainless steel hypodermic tubing with 0.0063 inch diameter #303

stainless steel wire passing through the tubing and extending 0.5

to 1.0 mm. for the olfactory bulb electrodes and 2.0 to 3.0 mm. for

the prepyriform electrodes. The electrodes were insulated with

Formvar and the recording surfaces were exposed by scraping clear

0.5 mm. of the barrel and 0.5 mm. of the tip, leaving insulation

between.

Olfactory bulb electrodes were inplanted under visual control

by observation of skull surface landmarks and subsequent histological

examination confirmed that all olfactory bulb electrodes were

appropriately placed. Three subjects were also implanted with

concentric bipolar electrodes straddling the ipsilateral prepyriform

cortex dipole generator layer by using the method of Freeman (1960).

In this procedure, single pulses from a Grass S-4 stimulator and

SIU-4 isolation unit were delivered across the olfactory bulb

electrode. The prepyriform electrode surfaces were connected for

monopolar recording from each surface by using Tektronix 122 pre­

amplifiers, with the evoked response from each surface displayed

5

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. on a channel of a Tektronix 564B storage oscilloscope- As the dipole

generator layer is approached and crossed, the evoked potential

recorded from the prepyriform electrode tip flattens and then inverts

to form a mirror image of the evoked potential recorded from the

barrel. This gives the investigator positive evidence that he has

one recording surface on each side of the generator layer (Freeman,

1960). The stereotaxic target for the beginning of stimulation was

as follows: anterior, 9.0 mm.; lateral, 4.5 mm.; and depth, 0-0 mm.

(Pellegrino & Cushman, 1967). Various types of miniature plugs were

used for connecting the electrodes to the recording equipment and

were attached to the skull with dental acrylic. Bicillin was

administered post-operatively to minimize the likelihood of infection.

During recording sessions, which began at least a week following

surgery, the subject was in a 15 cm. by 15 cm. by 15 cm. Plexiglas

box with a grid floor. The box was located on a shelf in a

refrigerator cabinet which was equipped with a fan for ventilation.

Connections were made from the rat to a Grass Model 7 polygraph

equipped with capacitor coupled preamplifiers and interfaced with

a Sanborn model 2000 frequency modulated tape recorder. The

polygraph provided an ink written time domain display of the lower

75 Hz. of the data stored on magnetic tape. The recording system

was calibrated with a digital voltmeter before each session. Once

amplifier gain settings were determined for a given session, they

were not changed; therefore the spectra obtained are relative but

not absolute. Recording began after exploratory behavior by the

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. subject had decreased to a low level. Baseline recording was carried

out until several sections of record with steady olfactory bulb slow

wave activity were obtained. Ketamine (Vetalar from the Parke-Davis

Co.) was administered by intraperitoneal injection in a dose of

50 mg/kg. At least five days intervened before Sodium Pentobarbital

(Fort Dodge Laboratories) was administered by intraperitoneal

injection in a dose of 60 mg/kg. Electrical activity from the

olfactory bulb electrode was simultaneously displayed on two channels

of the polygraph. One channel was set to display bulb slow wave

activity with the polygraph filters set at 0.15 and 3.0 Hz. The

second bulb channel was used to display fast activity with filter

settings of 10 and 75 Hz. for chart write-out and 10 and 500 Hz. for

the tape recorder. Prepyriform activity was displayed on another

channel using the same filter settings as for bulb fast activity.

Bipolar recording was done between the tip and barrel of the

electrodes.

The abstracting of approximately six second sections of

electrical activity for computer analysis was accomplished by

selection of records which showed steady olfactory bulb slow wave

activity. Once segments were selected for analysis, they were

saitpled and stored on computer compatible magnetic tape by a

Massey-Dickenson data acquisition system.

Spectra are analyzed to a maximum frequency of one-half the

rate of sampling of the analog signal. In order to avoid "aliasing"

(interference from harmonics), it is necessary to have all significant

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. spectral power within the range analyzed (Blackman & Tukey, 1959;

Walter, 1963; Jenkins & Watts, 1968, pp. 51-53). In this study, a

preliminary sampling rate of 300 Hz. was selected on the basis of

acute experiments in which the spectrum was analyzed to 350 Hz.

After the effect of Ketamine was observed, most Ketamine records

were resampled with a 420 Hz. sampling rate. The price one has to

pay for a higher sampling rate is that less real time is analyzed

for a given number of data points. Thus there is good reason for

not using a higher rate of sampling than necessary.

Each digital magnetic tape contained a number of individual

data records, and before the analysis began, certain sorting

operations were performed. As the lon£ file from the magnetic

tape was converted into one-record files for data analysis, the

number of "greater than full scale" codes, the number of blank

characters, and the identifiers which were inserted manually at

the time of analog to digital conversion were typed out on a teletype.

This allowed monitoring of all parts of the digital system. For

example, the occurrence of "blanks" on the tape indicated dirty tape

heads and that cleaning and resampling were necessary.

Spectral analysis was carried out in the time sharing mode of

the PDP-10 computer, losing a locally modified version of program

BMDX-92 "Time Series Spectral Estimation" from the Health Sciences

Computing Facility (University of California, 1969). The same

2048 data points in each record were analyzed into 8, 16, 32, and

64 frequency bands for window closing. This particular program does

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 9

not compute the autocovariances as an intermediate step in computing

the power spectrum. Since the covariances are not available, it was

concluded that cross-spectral analysis would not be prudent (Jenkins

& Watts, 1968, p. 396). With a sampling rate of 300 Hz., the digital

filter bandwidth for 32 bands was 4.7 Hz., and for a 420 Hz. sampling

rate, the filter bandwidth was 6.7 Hz. For a record of 2048 data

points, resolving the spectrum into 8, 16, 32, or 64 bands results

in 256, 128, 64, or 32 theoretical degrees of freedom respectively

for the spectral estimate (University of California, 1969). Instead

of accepting the theoretical degrees of freedom, window closing was

performed. Since the ends of this study were best served by trading

off high fidelity for high stability, the window closing procedure

led to the selection of the 32 band estimates for comparison.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. RESULTS

Differential spectral changes were observed following the

administration of Ketamine and Sodium Pentobarbital. Ketamine

injection was followed in three subjects at all electrode sites by

a dramatic increase in power above 100 Hz. The high frequency peaks

following Ketamine are centered at the same frequency within subjects

and at different frequencies across subjects. One subject showed no

change in the spectra obtained following Ketamine injection. It is

possible that this lack of effect was due to an ineffective intra­

peritoneal injection. Since Ketamine does not decrease muscle tone

and the eyes remain open, it is not possible to determine by visual

observation if the subject is anesthetized. Spectra obtained following

Sodium Pentobarbital injection show a reduction of spectral power at

frequencies below 100 Hz., with occasional increases at around 10 Hz.

Table 1 displays the changes observed following drug

administration. Spectral overlays of three spectra obtained before

and three spectra obtained 5 to 20 minutes after drug administration

are presented for each subject, drug, and electrode site in Figures

1-14 and discussed individually in the figure captions.

10

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. TABLE 1

SUMMARY OF SPECTRAL COMPARISONS

KETAMINE:

Subject Site Page Frequency Range in Hertz

<20 20-100 >100

PO B 21 - - +

B B 32 + +

P 33 - + +

J31 B 28 - + f

P 29 + +

A3 B 24 - - -

P 25 - --

SODIUM PENTOBARBITAL Subject Site Page Frequency Range in Hertz

<20 20-100 >100

PO B 20 - + -

BB 30 + -

P 21 + 4 -

J31 B 26 - + -

P 27 - - -

A3 B 22 - 4- -

P 23 + + -

Note.— B=olfactory bulb; P=prepyriform cortex; +=increase following drug administration; 4=decrease following drug adminis tration

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 1:

Subject PO, olfactory bulb spectra before (dashed lines)

and after Sodium Pentobarbital (solid lines). (The ordinate on all

the spectra is in relative units of natural logarithm of power

(microvolts/frequency band across an assumed one-ohm load).)

Post-drug spectral power was reduced between the 53.8 Hz. and the

80.6 Hz. bands and increased in the 20.2 Hz. to 33.6 Hz. bands.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 13

Figure 1

-&0-i

k x q 33*6 67.2 1008 1344 168.0 2QL6 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 2:

Subject PO, olfactory bulb spectra before (dashed lines)

and after Ketamine (solid lines). A large spectral increase oc­

curred in these records between the 134.4 Hz. and the 201.6 Hz.

frequency bands.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. without prohibited reproduction Further owner. copyright the of permission with Reproduced

-In P &54 0 2 0S 34 MA 2014 A IM 1344 10OS 12 < Figure 2 Figure z. H V ^ - V V 15 Figure 3:

Subject A3, olfactory bulb spectra before (dashed lines)

and after Sodium Pentobarbital (solid lines). The decrease

between 23.4 Hz. and 51.6 Hz. bands is associated with an

increase between the 9.4 Hz. and 18.8 Hz. bands and is similar

to the response observed for subject PO in Fig. 1.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 17

Figure 3

5jO= 53-=

6 3 =

7.0= & c7.5=: 7 8 0 =

8 5 = 9 0 =

* 5 =

1 Q O I I I I I | I I I I | I I I I I I I I I I I I I I I I I I I I I | 0 2 3 .4 46.9 7 0 3 9 8 8 11Z2 14016 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 4:

Subject A3, prepyriform cortex spectra before (dashed

lines) and after Sodium Pentobarbital (solid lines). The decrease

between the 46.9 Hz. and 70.3 Hz. bands is associated with an

increase in the range from the 0 Hz. to the 18.8 Hz. band. This

change occurred at a higher frequency range than did the change

observed at the olfactory bulb electrode shown in Fig. 3.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 19

Figure 4

Y Y Km > AV \

t+*7 * i ! . / /* .W!

4 6 .9 7 0 3 9 1 8 117.2 1 4 0 6 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 5:

Subject A3, olfactory bulb spectra before (dashed lines)

and after Ketamine (solid lines). It was stated in the text and

Table 1 that Ketamine did not have an effect on the electrical

activity of this subject and an ineffective intraperitoneal

injection was suggested- This implies that at the time of

recording, the drug dose was much smaller than for the other

subjects. In light of the lower effective dose for this subject,

the increase in the range of the 168 Hz- to 188.1 Hz. bands is

suggestive of a change in the high frequency range. The large

peak near 60.1 Hz. is a 60 Hz. artifact.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 21

Figure 5

5 0 —,

U

7J0-

&S-:

100 67.2 lOOul 1344 168J0 20L6 H z.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 6:

Subject A3, prepyriform cortex spectra before (dashed m lines) and after Ketamine (solid lines). No obvious change is

observed for this electrode site.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 23

Figure 6

7XH\

3>5-j

«OH

l Q O d 6Z2 KKX8 134.4 1680 20i6 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 7:

Subject J31, olfactory bulb spectra before (dashed lines)

and after Sodium Pentobarbital (solid lines). A broad decrease

is observed from 46.9 Hz. to the 84.4 Hz. band but there is no

low frequency increase as was seen in Fig. 1 and Fig. 3.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 25

Figure 7

50—,

5 5 ^

6 0 ^

70-1

90-1

O 703 117.8 140L6 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 8:

Subject J31, prepyriform cortex spectra before (dashed

lines) and after Sodium Pentobarbital (solid lines). A variable

increase is suggested between the 4.7 Hz. and 14.1 Hz. bands but

with only two records available after drug administration, this

is not conclusive.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 27

Figure 8

: * * 83-

93-

O 46.9 703 117.2 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 9:

Subject J31, olfactory bulb spectra before (dashed lines)

and after Ketamine (solid lines)- The major feature of this

comparison is the peak-from 121 Hz. on up. It is possible that

the 75 Hz. to 98.4 Hz. peak is an aliasing artifact. The center

frequency of the peak at 131.3 Hz. is the same as observed

at the prepyriform cortex electrode site shown in Fig. 10.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 29

Figure 9

\v

7 0 3 117.2 1406 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 10:

Subject J31, prepyriform cortex spectra before (dashed

lines) and after Ketamine (solid lines). The peak center at

131.3 Hz. is the same as for the olfactory bulb site shown in

Fig. 9.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 31

Figure 10

i a o 0 46.9 703 93L8 11Z2 1406 H z.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 11:

Subject B, olfactory bulb spectra before (dashed lines)

and after Sodium Pentobarbital (solid lines). The spectral

diminution between 65.6 Hz. and 75 Hz. is associated with an

increase from 4.7 Hz. to 13.4 Hz., similar to the changes seen

in Fig. 1 and Fig. 3.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 33

Figure 11

5 0 - n

*5-E

60-=

05-^

Z O =

Z 5 - =

8 0 = 8 0 =

9 0 =

*5-=

lO O -1 | | r -|~ r | i i i i | i i i i | i i i i | i i i i | i i ! i | i ! O 13* 409 703 939 117.2 1406

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 12:

Subject B, prepyriform cortex spectra before (dashed

lines) and after Sodium Pentobarbital (solid lines). The

decrease from 51.6 Hz. to 79.7 Hz. is associated with an

increase between 4.7 Hz. and 23.4 Hz., similar to Fig. 1, Fig.

and Fig. 11.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. without prohibited reproduction Further owner. copyright the of permission with Reproduced O |1 , |I 11 | IOC I .O 5 6 5^4

-In P ^ 3 6 4 o z 7. »4 4 o a 834 «o4 j 0-£ 3

3 4. 73 3 17 1403 1173 933 703 46.9 234 O ,1 II 1 1 1 1 I 1 11 1 | i 1 1 I I 1 1I I I I I Figure 12 Figure Hz. 35 Figure 13:

Subject B , olfactory bulb spectra before (dashed lines)

and after Ketamine (solid lines). The major peak centered at

134.4 Hz. and the minor peak between 80.6 Hz. and 100.8 Hz.

correspond closely to the changes observed for the prepyriform

cortex electrode in Fig. 14.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 13

& ° - n 5.Si

e o S

& 5 - =

7 j O i & c 7 j i I 80-=

9 0 l=

M i

10.0- I'1111| I1 I M | I I I I | I I I I | I | | | | | | | | | O 316 67.2 KXXS 1364 1680 20L6 Hz.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Fi glare 14:

Subject B, prepyriform cortex spectra before (dashed

lines) and after Ketamine (solid lines). The spectra obtained

are very similar to those obtained from the olfactory bulb site.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 14

5 3 - :

d X K 64-= h WK / P T l tl 0 . /JR\»

e 7 5 - = w r t a A-- T = l / m J/X x a o - E r hi \ >-♦-] J * . / \k'\ f\ \ i 1 \ » * * \w s. ,; * \\ ' * X \ ’/ l «. ‘ * 3 ./ \A\K

l O J O ^ 1 1 I 1 | 1 1 [" n r'i i T T lT"| I I I I I | rn i | i i 3 U 1 3 M I6k0 2016 Hx.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. DISCUSSION

The application of digital spectral analysis to the electrical

activity of the olfactory system of rats revealed differential

changes following drug administration. The two drugs, Ketamine and

Sodium Pentobarbital, are different in chemical class and are described

as producing dissimilar anesthetic states in man (Domino, Chodoff, &

Corssen, 1965).

The occasional increases in power around 10 Hz. following

barbiturate administration are consistent with the report of

Hem^ndez-PecJn et al. (1960) . The decrease below 100 Hz. would

be predicted (Pagano, 1966) if barbiturate administration was

followed by more shallow respiration by the subject. Recordings

of olfactory bulb slow wave activity, a known correlate of nasal

air flow (Gault & Leaton, 1963; Gault & Coustan, 1965), support

this interpretation. Figure 15 includes a section of bulb slow

wave activity obtained before barbiturate administration and a

section following administration. The post-barbiturate slow wave

record indicates that respiration is more shallow than during the

control period. Air flow cannot account for the entire barbiturate

effect, however; air flow changes are correlated with amplitude

changes, not waveform changes as are seen in the bulb fast activity

in Figure 15. Since the reticular formation is depressed by

barbiturates (Sharpless, 1970), perhaps reticular system depression

is responsible for the waveform changes. If waveform changes as

40

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 15:

Subject J31, oscillographic displays before and after

Sodium Pentobarbital administration. BSW is slow wave activity

recorded from the same electrode as BULB (olfactory bulb). PPYR

indicates the prepyriform cortex channel.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 42

Figure 15

1 s t e PRE

J 5 0 v vf i e m bsw ~Y yV ^v/'v^ /v /u ;V \A \. * y

J tb m 4bhi B U L B

I7Sjnafcm P P Y R

POST

JsOvvfam B S W

I* -— PPYR ^

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 43

are seen for the fast activity in Figure 15 were observed in a system

in forced vibration, the change would be described as an increase in

the damping factor of the system.

Air flow arguments do not hold for the case of high frequency

power increases following Ketamine administration; such changes were

not observed following manipulation of nasal air flow in acute rat

preparations initially anesthetized with Sodium Methohexital and

maintained with artifical ventilation following spinal cord

sectioning or injection (Orr, Gault, & Stewart,

1971). The power increases below 100 Hz. following Ketamine injection

for some subjects are correlated with an increase in nasal air flow.

Figure 16 includes a pre-Ketamine slow wave record and a post-Ketamine

slow wave record. The slow wave records show deeper respiration

following drug administration, thus a low frequency spectral increase

is consistent with Pagano's report (1966). Since Hughes and Hendrix

(1967) have reported increases in spectral power between 100 and

150 Hz. following odor stimulation of rabbits, the possibility of

olfactory stimulation must be considered. It seems unlikely that a

long-term (at least 20 minutes) change in the olfactory environment

would be correlated with the Ketamine injection. The possibility

that Ketamine in the bloodstream is an effective olfactory stimulant

would seem difficult to rule out.

A possible direction for future research would be to manipulate

conditions of nasal air flow directly before and after drug

administration. This would be possible in acute preparations where

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Figure 16:

Subject J31, oscillographic displays before and after

Ketamine administration. BSW is slow wave activity recorded

from the same electrode as BULB (olfactory bulb). PPYR indicates

the prepyriform cortex channel.

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 45

Figure 16 1 tie. PRE X i ^ b n B S W J jrafcm BULB

I p &PPYR W POST X vwSmBSW

I w o BULB Jȣ.PPYR

Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. 46

nasal air flow can be manipulated independently of respiration. This

would show whether the drug was affecting the background activity or

the olfactory spindle bursts. The development of a computer program

which computed the appropriate covariances would make practical the

investigation of multivariate spectra to determine frequencies shared

between the time series arising from different electrodes.

Ketamine was found to be unsuitable as an anesthetic for

olfactory research because of the high frequency spectral power

increase but may prove useful in electropharmacologic analysis of

olfactory system function.

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