Microbiology Quality Control introduction

When left undetected, micro- Content organisms can halt the production 208 Pharmaceutical Quality Control of pharmaceuticals, spoil food, ruin the Application Selector taste of beverages, negatively impact the performance of 209 Beverage Quality Control Application Selector computer chips, and cause serious illness. That’s why Pall is MICROBIOLOGY QUALITY CONTROL 210 Microbiology Quality dedicated to finding new and better ways to help you accurately Control Overview and reliably identify these microorganisms to protect both public 212 Microbiology Quality Control – Online Reference Library safety and the integrity of industrial assets. 213 Microbiology Quality Control Pall has been setting performance standards in Microbiology 213 Products – Membranes Quality Control laboratories for more than 50 years, and our 216 Products – Media products are referenced and recommended by regulatory 218 Products – Filter Funnels agencies worldwide. We invite you to explore the many ways 228 Products – Hardware Pall’s sample prep solutions can enhance your pharmaceutical 230 Products – Accessories and beverage quality control applications.

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 207 MICROBIOLOGY QUALITY CONTROL application selector 208 Pharmaceutical Quality Control Application Selector Application Control Quality Pharmaceutical aumpesr up 7 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 230,280 279 • 230,280 • • • • • • • • 224 • • 229 231 • • • • • • • • method.test regulated a of requirements the meet not may • or may listed media Culture * • • • 165 • • pumps Vacuum/pressure • 228 forceps steel Stainless • • • Pump Microbiology Sentino • 225a • dishes Petri • funnels filter MicroFunnel for manifolds funnel Filter • manifolds funnel Filter • • kits pad Absorbent • 226 Accessories and Hardware 216 • 222 • device vent Vacushield™ 216 220 218 Vents 216 216 216 funnels filter Magnetic Sentino • mL 300 and funnels,100 filter ST MicroFunnel mL 300 and funnels,100 filter Plus MicroFunnel mL 300 and funnels,100 filter MicroFunnel™ • funnels filter 216 magnetic mm 47 Funnels Filter • USP - Trypticasebroth Soy 213a Pseudomonas broth M-TGE broth M-Green YM broth MF-Endo broth M-FC broth indicator with TTC HPC 214 Supor Dispenser Filter Sentino™ filters,S-pack disc membrane PES Black Metricel Metricel GN-6 Membranes Ampoule Media* Ampoule It is the responsibility of the user to determine applicability in each situation. each in applicability determine to user the of responsibility the is It ® 0 E ebaeds itr,Spc 1 • • 215 filters,S-pack disc membrane PES 200 ® rt 1 • 216 broth C ebaeds itr,Spc 1 • • • • • 213 filters,S-pack disc membrane MCE

Page Number

Pseudomonas sp. Detection

Total Bacteria Detection

Total Coliforms Detection

Fecal Coliforms Detection

Yeast and Mold Detection Beverage Quality Control Application Selector

Membranes Page Number LactobacillusDetection sp. Total BacteriaDetection Total ColiformsDetection Fecal ColiformsDetection Yeast Detectionand Mold GN-6 Metricel® MCE membrane disc filters, S-pack 213 • • • • • Metricel Black PES membrane disc filters, S-pack 214 • • • • Sentino™ Filter Dispenser 213a • • • • • Supor® 200 PES membrane disc filters, S-pack 215 • •

Ampoule Media* HPC with TTC indicator broth 216 • M-FC broth 216 • MF-Endo broth 216 • M-Green YM broth 216 • M-TGE broth 216 • Orange Serum broth 216 • Trypticase Soy broth - USP 216 • application selector application Filter Funnels 47 mm magnetic filter funnels 226 • • • • • Microcheck® II beverage monitors 227 • • • • • Sentino Magnetic filter funnels 225a • • • • •

Vents Vacushield™ vent device 165 • • • • •

Hardware and Accessories Filter funnel manifolds 229 • • • • • Filter funnel manifolds for MicroFunnel™ filter funnels 228 • • • • • Petri dishes 230, 280 • • • • • Sentino Microbiology Pump 224 • • • • • MICROBIOLOGY QUALITY CONTROL Stainless steel forceps 230, 280 • • • • • Vacuum/pressure pumps 279 • • • • • * Culture media listed may or may not meet the requirements of a regulated test method. It is the responsibility of the user to determine applicability in each situation.

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 209 MICROBIOLOGY QUALITY CONTROL application overview 210 worldwide for meeting stringent microbial regulations. Supor regulations.microbial stringent meeting for worldwide give these products a reputation for quality and ease of use. of ease and quality for reputation a products these give that features design unique offering analysis, microbiological for funnels filter disposable of selection widest the offers Pall Funnels Filter Disposable MicroFunnel™ organisms. of growth consistent and uniform provide membranes These colonies. counting in ease for contrast background to characteristics binding protein and drug low from range that benefits additional provide membranes Black Metricel and Metricel GN ester cellulose recovery.and growthmicrobial accurate Pall’sfor mixed standards highest the to manufactured be must membranes safety.public and Analysis controlprocess for critical is identification and difficult, be can organisms sensitive Culturing Membranes require.you standards quality high the meet will products affordable,easy-to-use quality.our that Youconfident be can of certifications carry and literature, technical by supported are methods, testing international with comply products Our microorganisms. these control and detect to products of manufacture and development the in leader a is Pall them. control and detect to how knowing of importance the realize we in, live we world the share that organisms the of variety and presence the of awaremore become we As economics. industrial and safety public to critical is organisms micro- of identification and detection reliable accurate, The Results Reliable Rapid, Control Quality Microbiology labeled for added assurance of lot traceability.lot of assurance added for labeled and gamma-irradiated, bagged, individually is unit Each volume. sample your measure to easy it make to marked clearly are graduations volume and manifolds, laboratory standard to directly attach Funnels membrane. access to base from funnel of separation squeeze simple a permits easy.analysis make Design funnels filter MicroFunnel ® membrane has set the standard the set has membrane ® Rely on Pall Life Sciences to simplify your microbiological your simplify to Sciences Life Pall on Rely accuracy in the lab. the in accuracy productivity,speed, improve to and monitors beverage II beverageindustry chooseseasy-to-usethe Microcheck™ and food The chip. computer a on path circuitsub-micron a block can that contamination microorganism detect to quality microelectronicswater monitor to used is Monitor Quality Pall’sproduct. end mm quality 37 high safe, a preparing to essential are capabilities processingreliable and materials, raw of procedures,quality cleaning of effectiveness the Ensuring Products Microbiology Industrial procedures. analysis microbial your simplify to forceps steel stainless and Dispenser,Filter dishes, Sentino Petri Pump, Microbiology Sentino™ our like accessories hardware,and laboratory media, ampoule microbiological of line full a offers Pall Accessories and Hardware Designed Thoughtfully accessories. convenient hardware,and quality funnels, filter MicroFunnel disposable of line popular our with analysis Responding to Contamination Control With Disposable Funnels

The pharmaceutical microbiology market requires reliable and MicroFunnel Filter Funnels Demonstrate High accurate results as a basis for quality control of raw materials, Recovery of Test Organisms processes, and finished product. Critical decisions are made Test organism: daily and confidence in results, as well as methods to control Escherichia coli ATCC 11229 contamination during sampling and analysis, is a driving force % recovery % recovery in this market. A false positive on a test result can trigger a incubated in incubated by PN 4800 (GN-6 Metricel® base using removing to series of events that lead to unnecessary retesting, product membrane, 0.45 µm, TSB broth, MF-Endo broth, release delays, and extensive paperwork to document a white, gridded) 5 test units 5 test units course of action. This all leads to increased operating costs. Lot 1048L 98% 96% One way Pall Life Sciences has responded to contamination Lot 1382L 98% 94% control is by broadening our range of disposable filter funnels Lot 1878L 94% 98% with designs to help improve the sampling process. Whether the need is bioburden analysis, sterility testing, or water Test organism: Pseudomonas system monitoring, we have a MicroFunnel™ filter funnel aeruginosa ATCC 14207 to suit the application. Certified MicroFunnel disposable % recovery % recovery filter funnels are individually bagged, labeled, and ready incubated in incubated by to use. Each lot is evaluated for recovery performance. PN 4803 (Supor® base using removing to The standard selection of MicroFunnel filter funnels is ideal membrane, 0.2 µm, TSB broth, Pseudomonas white, gridded) 5 test units broth, 5 test units for any bioburden analysis.

Lot 1305L 97% 91% overview application Lot 1443L 97% 97% Lot 1179M 96% 101%

Test organism: Saccharomyces cerevisiae ATCC 4117 % recovery % recovery incubated in incubated by base using removing to PN 4805 (Metricel Black M-Green M-Green membrane, 0.45 µm, YM broth, YM broth, gridded) 5 test units 5 test units Lot 1197M 96% 101% Lot 1626M 100% 97% Lot 1933M 100% 103% MICROBIOLOGY QUALITY CONTROL

MicroFunnel Plus filter funnels streamline QC testing.

Simple, Versatile Contamination Control MicroFunnel Plus filter funnels are the only disposable filter funnels on the market that can serve as a sample cup and filter funnel in one product. This unique design further stream- lines sample collection and analysis of purified water systems while providing more protection to the integrity of the sample. This is a critical consideration for any contamination control program. Another important distinction of the MicroFunnel Plus filter funnel is its ability to sample water from systems maintained up to 90 ºC. The MicroFunnel ST filter funnel is packaged for ease of use when performing sterility testing within an isolator. Designed to be an acceptable sterility testing alternative and to reduce testing costs, the MicroFunnel ST filter funnel meets critical conditions of sterility testing and all requirements found in the U.S. Pharmacopeia, current edition.

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 211 Microbiology Quality Control – Online Reference Library

Pall’s website offers an extensive collection of product, MicroFunnel ST Filter Funnel Validation of Suitability technical, and application information. This valuable online for Sterility Testing Applications reference library features hundreds of technical articles, Monitoring for Bacteriological Contamination with posters, podcasts, application notes, and more that can help MicroFunnel Plus Filter Funnels you get the most out of your process. To view the following titles online – and many others – click the Literature Library Sentino™ Filter Dispenser Video link in the left sidebar when you visit www.pall.com/lab. Sentino Microbiology Pump Video Membrane Filter Technique Using MicroFunnel Plus Filter Funnels to Reduce Sample Contamination Microbiologist Discusses the Benefits of Using Disposable Filter Funnels for Contamination Control MicroFunnel™ Filter Funnels Performance Guide

Rapid, reliable test results are critical to effective online reference product quality monitoring. Accuracy in test results assures protection of the public while providing optimal control of a process. Pall’s line of products for rapid microbiology and sample prep provide solutions that speed the time it takes to collect a sample, analyze it, and produce test results. Designed to reduce the potential for sample contamination, our products are also convenient, easy-to-use, and economical.

At Pall, we’re responding to industry demands for rapid microbiological testing with revolutionary Enhancing capabilities in products that provide accurate, reproducible process control and bioburden analysis. Contact rapid micro us today to learn more. www.pall.com/biopharm MICROBIOLOGY QUALITY CONTROL

212 MICROBIOLOGY QUALITY CONTROL membranes 213a Applications dispense reliable design, Simple Dispenser Filter Sentino™ Techniquein: (MF) Filter Membrane the for Use the touch of a button. a of touch the at membrane of disc a dispenses aseptically keypad touch Soft instrument. the of jamming avoid to use during alignment in web refill keep and loading assist plates Guide use. during point any at pack refill of unloading and loading Simple disinfectants. laboratory standard with Sanitize anywhere. use for battery charge or source power main into plugged Use waste. material packaging less in resulting space storage minimize to sized pack refill Miniaturized workspace. arranging in flexibility provides and space top bench valuable frees footprint Small dispenser.reload to interruptions eliminate to discs membrane 200 hold to Designed intermediates, and final product. final and intermediates, materials, raw Pharmaceutical monitoring system water industrial and Pharmaceutical monitoring Beverage manufacturing productcare Personal monitoring recreationalwater and Environmental analysis water waste and Drinking Materials of Construction of Materials Storage: -15-45 °C (5-113 °F) (5-113 °C -15-45 Storage: °F) (59-86 °C 15-30 Operation: TemperatureRange lbs.) (2.0 kg 0.9 Weight: in.) (7.9 cm 20.0 Height: in.) (4.7 cm 12.0 Width: in.) (8.7 cm 22.0 Length: Dimensions Rubber Pads: Foot steel Stainless Retainer: Box with steel Stainless Clips: Retainer aluminum Anodized Plates: Guide aluminum Anodized Spools: aluminum Anodized Upright: Aluminum Plate: Bottom Polyester Label: and Keypad ABS- Polycarbonate Material: Housing tils te ocp . . Forceps Steel Stainless . Pump Microbiology Sentino . Funnels Filter Magnetic Sentino . . Dishes. Petri . . Funnels Filter MicroFunnel™ . . Microbiological Analysis for Media Ampoule Products Related Specifications Ordering Information Ordering atNme ecito Pkg Pkg 0.45 0.2 1/pkg Description 68121 North power America:Outside (1) Locations 68123 for Unit 1/pkg Number Part transformer,North power America:for (1) power Unit (1) Parts Replacement and Accessories Description 13183 13182 Number Part Dispenser Filter Sentino 82 0.8 0.45 68125 68124 anodized aluminum knobs aluminum anodized polymer blended (1) UK power cord with BS1363 plug, (1) User Guide CD Guide User plug,(1) BS1363 with cord power UK (1) plug, 7/7 CEE with transformer,cord power European (1) CD Guide User plug,(1) 5-15P NEMA with cord μ μ ,Mtie lc ebae1000/pkg membrane Black m,Metricel m,Supor μ μ ,Mtie lc ebae 1000/pkg membrane Black m,Metricel Metricel m,GN-6 ® ebae 1000/pkg membrane ® ebae1000/pkg membrane 13182: IEC 320 connector and NEMA and connector 320 IEC 13182: Cord Power Plug): Entry (Power Interface Output socket 3-pin 320 IEC Interface: Input Hz 47-63 VAC,100-240 Amp 1.6 VDC 24 TransformerPower AC/DC V Voltage:24.0 Supply DC hydride) Type:metal (nickel NiMH V Voltage:12.0 Battery 13183: IEC 320 connector and connector 320 IEC 13183: connector and UK BS1363 plug BS1363 UK and connector 5-15P grounded plug grounded 5-15P jack barrel mm 2.5 European CEE 7/7 plug, IEC 320 IEC plug, 7/7 CEE European .225,274 . 225,274 . 224 . 225a . 218-223 .217 GN-6 Metricel® MCE Membrane Disc Filters Certified 0.45 µm membrane for microbiological analysis meets or surpasses regulatory requirements

Specifications

Filter Media Extractables - Boiling Water Hydrophilic mixed cellulose esters < 2% Pore Size Minimum Bubble Point - Water 0.45 µm 1.8 bar (180 kPa, 26 psi) Typical Thickness Recovery 152 µm (6 mils) (measured vs. control) > 90% E. coli Typical Filter Weight 4 mg/cm2 Refractive Index 1.512 Typical Water Flow Rate > 65 mL/min/cm2 at 0.7 bar Gamma-Irradiated (70 kPa, 10 psi) Provided gamma-irradiated or non- Mixed cellulose esters is the most sterile. Validated dose 15 - 30 kGy. Maximum Operating Temperature – accepted filter media for micro- Autoclavable if desired at Water biological analysis, and provides 121 - 123 °C (250 - 253 °F) at 74 °C (165 °F) maximum recovery of organisms. 1.0 bar (100 kPa, 15 psi) for Unique dot grid pattern provides Typical Moisture Pick-Up 15 - 20 min. guidance for quantification of < 1% after 24 hr at 48% relative

bacterial colonies without growth humidity at 23 °C (73 °F) membranes inhibition or enhancement. Suitable for microbiological analysis Ordering Information using the Membrane Filter (MF) Technique. GN-6 Metricel Membrane Disc Filters, 0.45 µm (S-Packs) Part Number Description Pkg Variety of packaging options meets any need. Available in gamma- 66265 47 mm, plain, gamma-irradiated 200/pkg irradiated, individually packaged 66278 47 mm, grid, gamma-irradiated 200/pkg S-packs, autoclave packs, or 66068 47 mm, grid, gamma-irradiated 1000/pkg

non-sterile packs. 66678 47 mm, grid side down, gamma-irradiated 1000/pkg MICROBIOLOGY QUALITY CONTROL Use with Pall microbiological ampoule 66191 47 mm, grid, gamma-irradiated 2000/pkg media for high growth levels and easy 66539 50 mm, grid, gamma-irradiated 200/pkg identification of contaminants. 60016 85 mm, grid, gamma-irradiated (not individually packed) 50/pkg

Applications GN-6 Metricel Membrane Disc Filters, 0.45 µm (Non-Sterile Packages) Part Number Description Pkg Certified for the microbiological analysis of potable, waste, process, 63066 13 mm, plain 100/pkg and natural waters in accordance 63068 25 mm, plain 100/pkg with the MF Technique referenced 64191 25 mm, grid 100/pkg in Standard Methods for the 64382 37 mm, plain, with support pads 100/pkg Examination of Water and Waste- 63069 47 mm, plain 100/pkg water, current edition, and the U.S. EPA's Microbiological Methods for 63020 47 mm, grid 100/pkg Monitoring the Environment, 66536 142 mm, plain 25/pkg 600/8-78-017. Ideal for isolation and enumeration Related Products of Total and Fecal Coliforms, E. coli, MicroFunnel™ Filter Funnels ...... 219 - 223 Fecal Streptococcus, fungi, and other heterotrophic organisms.

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 213 MICROBIOLOGY QUALITY CONTROL membranes 214 Contrasting membrane for microbiological analysis microbiological for membrane Contrasting Metricel Applications for critical applications. critical for (S-packs) packs gamma-irradiated individual in or non-sterile Available counting. and viewing while guidance provides line grid white and membrane black between contrast Sharp edition. current Wastewater,Waterand of nation Exami- the for Methods Standardin T (MF) Filter Membrane the in use for Certified growth. colony inhibit or enhance not does pattern grid dot Exclusive labs. microbiology in colonies opaque counting for contrast excellent providesbackground Dark quality control laboratories. control quality beverage and food in identification and monitoring organism Spoilage colonies. mold and yeast of enumeration and isolation the for membrane Excellent echnique as echnique ® Black PES Membrane Disc Filters Disc Membrane PES Black described Filter Media Filter 0.8 µm: > 102 > µm: 0.8 34 > µm: 0.45 mL/min/cm TypicalRate WaterFlow mils) (5.8 µm 147 µm: 0.8 mils) (5.1 µm 130 µm: 0.45 TypicalThickness µm 0.8 and 0.45 Size Pore black polyethersulfone, modified Hydrophilic aumPesr up . Pumps Vacuum/Pressure . . Forceps Steel Stainless . Funnels Filter Magnetic Sentino™ . Dishes. Petri . Microbiological Analysis for Media Ampoule . . Kits Pad Absorbent . Funnels Filter Magnetic mm 47 Products Related Specifications Ordering Information Ordering 68 . m 7m,gi 100/pkg 100/pkg 100/pkg 100/pkg Pkg 200/pkg mm,µm,grid 47 0.8 200/pkg mm,(S-pack) µm,grid,47 gamma-irradiated 0.8 mm,µm,grid 47 0.45 mm,(S-pack) µm,grid,47 gamma-irradiated 0.45 mm,µm,grid 25 0.8 66588 mm,µm,grid 25 0.45 66587 66586 Description 66585 60065 60138 Number Part Filters Disc Membrane Black Metricel (70 kPa, 10 psi) 10 kPa, (70 2 at 0.7 bar 0.7 at 15 - 20 min. 20 - 15 for psi) 15 kPa, (100 bar 1.0 at °F) 253 - (250 °C 123 - 121 at desired if Autoclavable kGy.30 - 15 Validateddose sterile. non- or gamma-irradiated Provided Gamma-Irradiated psi) 15 kPa, (100 bar 1.0 µm: 0.8 psi) 23 kPa, (160 bar 1.6 µm: 0.45 Water– Point Bubble Minimum .230,280 .231 . 230,280 . 279 . 226 .225a .217 Supor® 200 PES Membrane Disc Filters Highly retentive, certified membrane for isolation and enumeration of organisms

Specifications

Filter Media Extractables - Soxhlet Extraction Hydrophilic polyethersulfone < 4% Pore Size Minimum Bubble Point - Water 0.2 µm 3.5 bar (350 kPa, 51 psi) Diameter Minimum Recovery 47 mm > 90% (measured vs. controlled) Typical Thickness Refractive Index 145 µm (5.7 mils) 1.640 Typical Water Flow Rate Gamma-Irradiated > 19 mL/min/cm2 at 0.7 bar Provided gamma-irradiated. Validated (70 kPa,10 psi) dose 15 - 30 kGy. Autoclavable if Ensures accurate analysis. Exclusive desired at 121 - 123 °C (250 - 253 °F) Maximum Operating Temperature - dot grid pattern neither inhibits nor at 1.0 bar (100 kPa, 15 psi) for Water enhances microbial growth. 15 - 20 min. 100 °C (212 °F) Certified for use in the MF Technique as described in Standard Methods for the Examination of Water and Ordering Information Wastewater, current edition. Supor 200 Membrane Disc Filter Reduces filtration time. Superior flow Part Number Description Pkg membranes rates and high throughputs provide fast, easy filtration. 66234 0.2 µm, 47 mm, grid, gamma-irradiated (S-pack) 200/pkg

Applications Related Products 47 mm Magnetic Filter Funnels ...... 226 Ideal choice for microbiology labs Absorbent Pad Kits ...... 231 requiring isolation and enumeration Ampoule Media for Microbiological Analysis ...... 217 of organisms stunted in size from Petri Dishes...... 230, 280 exposure to harsh conditions.

Sentino™ Magnetic Filter Funnels ...... 225a MICROBIOLOGY QUALITY CONTROL Easily isolate and enumerate Stainless Steel Forceps ...... 230, 280 Pseudomonas species, especially Vacuum/Pressure Pumps ...... 279 stressed organisms, found in process water and other fluid samples (like those analized in the electronics and pharmaceutical industries).

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 215 MICROBIOLOGY QUALITY CONTROL media 216 Wide variety of sterile ampoule media pre-measured for efficiency and convenience and efficiency for pre-measured media ampoule sterile of variety Wide Analysis Microbiological for Media Ampoule * Requirements referencedin Requirements * and Wastewater, and Water of Examination the for Methods wide-mouth glass ampoules. glass wide-mouth with media the of pouring Simplify applications. your for ampoules glass or plastic between Choose media. presterilized and premixed with efficiency Maximizes ampoules.* mL 2 requirementswith TechniqueMethod Standard (MF) Filter Membrane Meets ampoules per box. per ampoules 50 with packaged economically is media ampoule plastic All current edition. current Standard and microelectronicsindustries. and pharmaceutical, beverage, and food water,municipal in use for broths ent nutri- selective, of variety a in available is media ampoule Sciences Life Pall Applications contamination in water samples. water in contamination detecting for media selective highly arebroths KF-Streptococcal General: microelectronicsindustries. and pharmaceutical the in bacteria total for waters process and products final test to used be may HPC and M-TGE, broth, Microelectronics: and Pharmaceutical organisms. spoilage potential detect to products) these of manufacture the in used fluids (and products final QC to industry beverage and food the in used arebroths Serum Orange and YM, M-Green Beverage: and Food and Coliforms, Totalfor Fecal samples Coliforms, water analyzing when used are media acid) rosolic without and (with Water:Drinking Wastewaterand Municipal Pseudomonas and Pseudomonas E. coli E. MF-Endo and M-FC and MF-Endo TrypticaseSoy . M-TGE, HPC, M-TGE, 24 hr. culture @ 35 ºC (95 ºF) (95 hr.ºC 24 35 @ culture Broth,MF-Endo 24 hr. culture @ 44.5 ºC (112 ºF) (112 hr.ºC 24 44.5 @ culture Broth,M-FC ºF) (95 hr.ºC 24 35 @ culture Broth,M-TGE ºF) (95 hr.ºC 48 35 @ culture Broth,Pseudomonas E. coli E. S.epidermidis E. coli E. P.aeruginosa Specifications Target Recovery Test Shelf Life Target Colony Broth Organism(s) (vs. Control) Organism(s) pH at 25 °C (2 - 8 °C) Media Color Color MF-Endo Total Coliforms > 85% E. coli 7.2 ± 0.2 1 year pinkish red dark red with metallic sheen M-FC Fecal Coliforms > 85% E. coli 7.4 ± 0.2 1 year blue blue M-FC with Fecal Coliforms > 85% E. coli 7.4 ± 0.2 1 year violet blue Rosolic Acid* M-TGE Total Bacteria > 85% E. coli; 7.0 ± 0.2 1 year* pale yellow organism S. epidermidis dependent M-TGE with TTC Total Bacteria > 85% E. coli 7.0 ± 0.2 1 year pale yellow red Indicator Trypticase Soy - USP Total Bacteria > 85% E. coli; 7.3 ± 0.2 1 year pale yellow organism S. epidermidis dependent KF-Streptococcal Fecal > 85% S. faecalis 7.2 ± 0.2 1 year light purple red Streptococcus Pseudomonas Pseudomonas sp. > 85% P. aeruginosa 7.1 ± 0.2 1 year light amber green-blue M-Green YM Yeasts and Molds > 85% S. cerevisiae 4.6 ± 0.2 1 year** green pale green Orange Serum Lactobacillus, Acid > 85% L. plantarum; 5.6 ± 0.2 1 year dark amber organism Resistant Bacteria S. cerevisiae dependent HPC with Total Bacteria > 85% E. coli; 7.1 ± 0.2 1 year pale yellow red TTC Indicator S. epidermidis *Rosolic acid is a selective agent that helps increase the specificity of the medium for Fecal Coliforms. **Does not require refrigeration during the one-year shelf life. media

Ordering Information

Microbiological Media, 2 mL Plastic Ampoules Microbiological Media, 2 mL Wide-mouth Glass Ampoules Part Number Description Pkg Part Number Description Pkg 68105 MF-Endo broth, Total 50/pkg 68100 M-FC broth, Fecal 20/pkg Coliforms Coliforms MICROBIOLOGY QUALITY CONTROL 4302 M-FC broth with rosolic 50/pkg 68101 M-FC broth with rosolic 20/pkg acid, Fecal Coliforms acid, Fecal Coliforms 68106 M-TGE broth, Total 50/pkg 68102 MF-Endo broth, Total 20/pkg Bacteria Coliforms 68111 M-TGE with TTC indicator, 50/pkg Total Bacteria Microbiological Media, 100 mL Bottle 4307 Trypticase Soy broth - 50/pkg Part Number Description Pkg USP, Total Bacteria 4313 MF-Endo broth, Total 1/pkg 68108 KF-Streptococcal broth, 50/pkg Coliforms, bottle Fecal Streptococcus 4306 Pseudomonas broth, 50/pkg Pseudomonas sp. Related Products 68107 M-Green YM broth, yeast 50/pkg 37 mm Quality Monitors...... 225 and mold Absorbent Pad Kits...... 231 68109 Orange Serum broth, 50/pkg GN-6 Metricel® MCE Membranes ...... 213 Lactobacillus sp. Metricel Black PES Membranes ...... 214 4352 HPC Media with TTC 50/pkg Microcheck II Beverage Monitors ...... 227 indicator, Total Bacteria MicroFunnel™ Filter Funnels ...... 219 - 223 Petri Dishes ...... 230, 280 Sentino™ Microbiology Pump ...... 224 Stainless Steel Forceps ...... 230, 280 Supor® 200 PES Membranes ...... 215

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 217 MICROBIOLOGY QUALITY CONTROL filter funnels 218 disposable filter funnels filter disposable ready-to-use convenient, with efficiency laboratory Increase Funnels Filter MicroFunnel™ Variety of options. Choose from from Metricel GN-6 Choose options. of Variety sterilization. EtO with associated extractables toxic for potential the eliminate to gamma-irradiated and Pre-assembled use. to Ready funnels. reusable requiredwith sterilization and cleaning the nates elimi- design Disposable time. Saves increments. mL 50 in marked is funnel visibility.mL higher 300 The for funnel the around completely marked is increment mL 100 The increments. mL 10 in marked are funnels mL 100 The measurement. sample accurate and easy Allows samples. larger process to capacity mL 300 in Available samples. of cross-contaminationprevent units filter disposable bagged, Individually membrane. to access easy allows base from cylinder of separation squeeze Unique use. to Easy results.reliable of assurance added provide to analysis microbiological for certified is lot Each Certified. pore size, pore culturing on agar or broth medium. broth or agar on culturing for membrane the remove Easily size. pore membrane Black Metricel or size, pore µm 0.45 or 0.2 Supor ® membrane in 0.45 µm 0.45 in membrane ® with 0.45 or 0.8 µm 0.8 or 0.45 with membrane with Applications 5. Cover,incubate. 5. and invert, broth- the onto filter membrane the Place 4. base the from filter membrane the Remove 3. perform removed, kit lid dish Petri With 2. of ampoule an of contents the Dispense 1. (With Petri Dish Lid Kit) Lid Dish Petri (With Funnel Filter LP MicroFunnel for Procedures Coliforms or Coliforms Totalas such contaminants, bacterial of Testvariety a for results. the in confidence and sensitivity improve can samples larger Processing funnels. smaller of wait" and "pour the without samples larger processing for ideal is funnel mL 300 The sterilized. be hardwarecannot reusable and down breaks autoclave the case in funnels filter MicroFunnel of supply a Maintain week. work or day the of end the at samples last-minute for Convenient traceability.lot for labeled Individually production. pharmaceutical in used fluids aqueous of analysis control quality for Ideal light-colored colonies. light-colored other and yeast counting for contrast better a provides membrane Black Metricel with funnel filter MicroFunnel sizes. pore µm 0.2 and 0.45 the between choosing by Technique. (MF) Filter Membrane the of principles the using contamination microbial for Testsolution aqueous any lid kit. lid dish Petri the in pad absorbent soaked forceps. with cylinder. funnel the of top the near squeezing gently by membrane access then filtration 4810. PN funnel, filter LP MicroFunnel with only supplied kit, lid dish Petri the in pad absorbent the onto medium culture Pseudomonas species, Dimensions cm 13.46 Area Filtration Effective Cellulose Pad: Support Polyethylene Adapter: Funnel Polyethylene Plug: Polystyrene Cover: Base: Dish Cylinder,Petri Lid, Base, (hydrophilic Supor Media: Filter Construction of Materials Specifications Validated dose 15 - 30 kGy 30 - 15 Validateddose Gamma-Irradiated only) use (vacuum Hg) in. (25 Hg cm 63.5 VacuumMaximum Diameter: Height: Funnel mL 300 Diameter: Height: Funnel mL 100 Polypropylene membranes polyethersulfone) (modified Black Metricel and esters), cellulose (mixed polyethersulfone), 8.8 cm (3.5 in.) with cover with in.) (3.5 cm 8.8 cover with in.) (3.6 cm 9.1 cover with in.) (2.4 cm 6.1 lid dish Petri with in.) (2.5 cm 6.4 cover with in.) (3.0 cm 7.6 lid dish Petri with in.) (3.2 cm 8.1 2 GN-6 Metricel Ordering Information MicroFunnel™ Filter Funnels, 100 mL Accessories and Replacement Parts Part Number Description Pkg Part Number Description Pkg 4800 0.45 µm GN-6 Metricel® membrane, 50/pkg 4701 Autoclavable adapters for vacuum 3/pkg white, gridded, individually bagged manifold when using rubber stopper 4804 0.45 µm GN-6 Metricel membrane, 200/pkg 4713 Vacuum pump adapter for 1/pkg white, gridded, individually bagged MicroFunnel filter funnel with ◆ 4810 LP unit with 0.45 µm GN-6 Metricel 50/pkg Milliflex PLUS pump membrane, white, gridded, 4895 Lid kit accessory for culturing 20/pkg individually bagged, Petri dish lid kit membrane in place 4801 0.45 µm GN-6 Metricel membrane, 50/pkg 15408 1-place aluminum manifold 1/pkg white, gridded, unbagged 15411 3-place aluminum manifold 1/pkg ® 4803 0.2 µm Supor membrane, white, 50/pkg 15413 6-place aluminum manifold 1/pkg gridded, individually bagged 13157 Vacuum/pressure pump, 115 V 1/pkg 4806 0.2 µm Supor membrane, white, 50/pkg no grid, individually bagged 13158 Vacuum/pressure pump, 230 V 1/pkg 4852 0.45 µm Supor membrane, white, 50/pkg 4690 Forceps with multi-colored grips 3/pkg gridded, individually bagged 4805 0.45 µm Metricel Black membrane, 50/pkg black, gridded, individually bagged Related Products

Ampoule Media for Microbiological Analysis...... 217 funnels filter MicroFunnel Filter Funnels, 300 mL Petri Dishes ...... 230, 280 Part Number Description Pkg Sentino™ Microbiology Pump ...... 224 4815 0.45 µm GN-6 Metricel membrane, 20/pkg white, gridded, individually bagged 4818 0.2 µm Supor membrane, white, 20/pkg gridded, individually bagged 4828 0.45 µm Supor membrane, white, 20/pkg gridded, individually bagged 4817 0.45 µm Metricel Black membrane, 20/pkg gridded, individually bagged 4819 0.8 µm Metricel Black membrane, 20/pkg MICROBIOLOGY QUALITY CONTROL gridded, individually bagged

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 219 MICROBIOLOGY QUALITY CONTROL filter funnels 220 tils te ocp ...... 230,280 Forceps Steel Stainless ...... 224 Pump Microbiology Sentino™ ...... 230,280 Dishes Petri 229 - ...... 228 Manifolds Funnel Filter ...... 217 Microbiological Analysis for Media Ampoule Products Related contamination of risk the eliminate to monitoring system water revolutionizes cup sample and funnel filter Combined Funnels Filter Plus MicroFunnel™ Supor with products Plus MicroFunnel lid. remove to need No port. sample lid the through sample the of collection aseptic allows unit AP Plus MicroFunnel contamination. introducing risk and funnel disposable to cup from sample transfer to need No one. in all funnel filter and container sample as performs Product lot traceability.lot and control contamination for labeled and bagged Individually volume. sample your measuring in ease for marked clearly areVolume graduations stopper.and adapter or manifold laboratory standard a to directly Attaches filtration. during funnel the into drawn is contamination airborne no ensuresVent filter contamination. introducing risk and funnel the open to having without filtration allows and seal liquid-tight a to snaps Ventedlid ºC. 90 to up water hot of sampling allow to ® membrane are designed are membrane use with use MicroFunnel Plus Filter Funnel Features Funnel Filter Plus MicroFunnel Materials of Construction of Materials Plug: Polyethylene (PN 4807, 4808) 4807, (PN Polyethylene Plug: Polyethylene Adapter: Polyethylene Bag: 13.46 cm 13.46 Area Filtration Effective with Polyethylene Cover: Funnel Urethane Gasket: Adhesive Polypropylene Base: and Funnel Cellulose Pad: Support (mixed Metricel GN-6 Media: Filter Specifications Applications Performance reduce the steps in sample collection and testing. and collection sample in steps the reduce Save yourself time, avoid sample contamination or mix-ups, and mix-ups, or contamination sample avoid time, yourself Save

Improves the efficiency of monitoring of efficiency the Improves your water system while reducing the reducing while system water your introduction of contamination. of introduction Eliminates steps in sample collection sample in steps Eliminates and testing that could potentially could that testing and contaminate your sample. your contaminate support) non-woven a on copolymer (acrylic hydrophobic Versaporhydrophobic membranes polyethersulfone) (modified Black Metricel and polyethersulfone), (hydrophilic Supor esters), cellulose 2 ® membrane Fits directly onto filter manifold. filter onto directly Fits separate sample collection cup. collection sample separate for need eliminates funnel filter Capped leaking. prevents interface base-to-funnel Sealant filtration. during protected and sealed remain to sample allows Vent Diameter: 6.1 cm (2.4 in.) with cover with in.) (2.4 cm 6.1 Diameter: cover with in.) (3.0 cm 7.6 Height: Funnel mL 100 Dimensions Validated dose 15 - 30 kGy 30 - 15 Validateddose Gamma-Irradiated 4843, 4823, 4814, 4813, 4809, PN Ambient 4829: 4808, 4807, PN Temperature Collection Sample Maximum only) use (vacuum Hg) in. (25 Hg cm 63.5 VacuumMaximum in.) (3.4 cm 8.7 Diameter: in.) (3.5 cm 8.9 Height: Funnel mL 300

Gridded 0.45 µm GN-6 Metricel GN-6 µm 0.45 Gridded membrane is ideal for analysis of analysis for ideal is membrane ambient water by Membrane Filter Membrane by water ambient (MF) Technique.(MF) MicroFunnel Plus with 0.45 or 0.2 µm 0.2 or 0.45 with Plus MicroFunnel Supor membrane is designed for designed is membrane Supor sampling hot water (such as that as (such water hot sampling found in hot loop WFI systems). WFI loop hot in found and 4844: 90 °C (194 °F) (194 °C 90 4844: and

® How to Use the MicroFunnel™ Plus Filter Funnel Sampling Ambient Water

1. Carefully remove the 2. Securely snap lid in 3. Remove membrane vent 4. Gently grasp funnel then vented lid and collect place to prevent sample from base, place funnel remove and discard lid. the sample. loss. Transport sample to directly onto manifold, laboratory for filtration. and filter the sample.

Collecting Hot Water Samples

5. Release cylinder from 6. Remove the membrane, 1. Place funnel into holder 2. With funnel and holder base by squeezing the plate the filter, and with graduations visible resting on a firm, level midpoint of the cylinder. incubate. through the viewing surface, securely snap lid funnels filter slot. Carefully remove in place to prevent sample the vented lid and loss. Remove funnel from Note: When using this product for sampling hot water, observe collect the hot water holder and transport safety precautions. This includes the use of insulated rubber sample. sample to laboratory for gloves, safety glasses, and Pall Life Sciences funnel holder filtration. (Proceed with (PN 4824 for 100 mL funnels and PN 4825 for 300 mL funnels). steps 3-6 for ambient water.)

Ordering Information MicroFunnel Plus Filter Funnels, 100 mL MicroFunnel Plus AP Filter Funnels, 100 mL

Part Number Description Pkg Part Number Description Pkg MICROBIOLOGY QUALITY CONTROL 4807* 0.45 µm GN-6 Metricel® 50/pkg 4843 0.2 µm Supor membrane, 50/pkg membrane, white, gridded, white, gridded, individually bagged individually bagged (not compatible 4856 0.45 µm GN-6 Metricel membrane 50/pkg with hot water applications) white, gridded, individually bagged 4808* 0.45 µm Metricel Black membrane, 50/pkg 4844 0.45 µm Supor membrane, 50/pkg gridded, individually bagged (not white, gridded, individually bagged compatible with hot water applications) 4809 0.2 µm Supor® membrane, 50/pkg MicroFunnel Plus AP Filter Funnels, 300 mL gridded, individually bagged Part Number Description Pkg 4823 0.45 µm Supor membrane, 50/pkg 4855 0.45 µm Supor membrane, 20/pkg white, gridded, individually bagged white, gridded, individually bagged

MicroFunnel Plus Filter Funnels, 300 mL Accessories and Replacement Parts Part Number Description Pkg Part Number Description Pkg 4813 0.2 µm Supor membrane, 20/pkg 4824 Funnel holder, 100 mL 1/pkg white, gridded, individually bagged 4825 Funnel holder, 300 mL 1/pkg 4857 0.2 µm Supor membrane, white, 20/pkg 4701 Autoclavable adapters for vacuum 3/pkg gridded manifold when using rubber stopper 4814 0.45 µm Supor membrane, 20/pkg 4713 Vacuum pump adapter for using 1/pkg white, gridded, individually bagged MicroFunnel filter funnel with 4829* 0.45 µm GN-6 Metricel membrane, 20/pkg Milliflex PLUS◆ pump white, gridded, individually bagged 4895 Lid kit accessory for culturing 20/pkg (not compatible with hot water membrane in place applications) 4845 MicroFunnel Plus AP sampling 50/pkg tubes, individually bagged *For use with ambient temperature samples only. 1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 221 MICROBIOLOGY QUALITY CONTROL filter funnels 222 Applications isolators within testing sterility for choice economical and Convenient Funnels Filter Disposable ST MicroFunnel™ industry. pharmaceutical the in applications testing sterility for choice a provides sterility testing. sterility for Pharmacopeias European and Japan, U.S., requirementsof Meets easy.retrieval membrane makes design separation squeeze Simple airlock. through transfer easy ensures packaging bagged Double solutions. antibiotic testing for ideal is membrane Supor or low binding Supor binding low or 0.45 µm GN-6 Metricel GN-6 µm 0.45 suite. containment or isolator an using when testing sterility closed-system costly to alternative an offer funnels filter ST MicroFunnel product for release. for product final of testing control Quality production. pharmaceutical in used fluids aqueous of analysis control quality for Ideal Technique.(MF) Filter Membrane the of principles the and funnels filter disposable ST MicroFunnel using contamination microbial for Testsolution aqueous any ® membrane ® membrane Materials of Construction of Materials Diameter: 8.8 cm (3.5 in.) (3.5 cm 8.8 Diameter: in.) (3.6 cm 9.1 Height: Funnel mL 300 in.) (2.4 cm 6.1 Diameter: in.) (3.0 cm 7.6 Height: Funnel mL 100 Dimensions cm 13.46 Area Filtration Effective Cellulose Pad: Support Polyethylene Bag: and Adapter Funnel Polystyrene Cover: Funnel Polypropylene Base: and Cylinder (hydrophilic Supor Media: Filter aumPesr up . . Pumps Vacuum/Pressure . . Forceps Steel Stainless . Pump Microbiology Sentino™ . . Manifolds Funnel Filter . . Microbiological Analysis for Media Ampoule Products Related Specifications Ordering Information Ordering 83Mcounl30S ntwt . m 20/pkg 40/pkg 20/pkg 40/pkg µm 0.2 with unit ST 300 MicroFunnel Pkg 20/pkg µm 0.2 with unit ST MicroFunnel 40/pkg Parts Replacement and Accessories µm 0.45 with unit ST 300 MicroFunnel µm 0.45 with unit ST MicroFunnel 4853 µm 0.45 with unit ST 300 MicroFunnel 4851 µm 0.45 with unit ST MicroFunnel 4812 4811 Description 4751 4750 Number Part Funnels Filter Disposable ST MicroFunnel atNme ecito Pkg 1/pkg 3/pkg filter MicroFunnel using for adapter pump Vacuum when manifold vacuum for adapters Autoclavable Description 4713 4701 Number Part (mixed cellulose esters) membranes esters) cellulose (mixed Metricel GN-6 and polyethersulfone) 2 funnel with Milliflex PLUS Milliflex with funnel stopper rubber using membrane,mL gridded,Supor 300 membrane,mL gridded,Supor 100 membrane,mL gridded,Metricel 300 GN-6 membrane,mL gridded,Metricel 100 GN-6 membrane,mL plain,Supor 300 membrane,mL plain,Supor 100 ◆ bag; 4 overpack bags per box. per bags overpack 4 bag; overpack an within funnels bagged individually 5 box: per total units 20 Funnel mL 300 box. per bags overpack 4 bag; overpack an within funnels bagged individually 10 box: per total units 40 Funnel mL 100 Note Packaging kGy.30 - 15 Validateddose Gamma-Irradiated only) use (vacuum Hg) in. (25 Hg cm 63.5 VacuumMaximum pump ...... 228 - 229 - . 228 . 230,280 . 279 .224 .217 MicroFunnel™ Filter Funnels With Polycarbonate Membrane Ideal for concentrating organisms in fluid samples

Specifications

Materials of Construction 300 mL Funnel Filter Media: Polycarbonate with PVP Height: 9.1 cm (3.6 in.) with cover (polyvinylpyrrolidone) wetting agent 8.9 cm (3.5 in.) without cover Support Pad: Cellulose Diameter: 8.8 cm (3.5 in.) with cover Funnel and Base: Polypropylene 8.5 cm (3.4 in.) without cover Funnel Cover: Polystyrene Maximum Vacuum Funnel Adapter: Polyethylene 635 mm Hg (25 in. Hg) Membrane Diameter (vacuum use only) 47 mm Gamma-Irradiated Pore Size Validated Dose: 15 - 30 kGY 0.4 µm (1.5 - 3.0 MRad)

Flat, smooth surface allows for easy Effective Filtration Area elution of organisms or particulate. 13.46 cm2 Collection of organisms or Dimensions particulate on one planar surface is 100 mL Funnel ideal prior to electron microscopy Height: 7.6 cm (3.0 in.) with cover (SEM) or light microscope (LM) 7.3 cm (2.9 in.) without cover techniques. Diameter: 6.1 cm (2.4 in.) with cover

Ready-to-use, disposable design 5.7 cm (2.3 in.) without cover funnels filter eliminates sample cross- contamination. Ordering Information MicroFunnel Filter Funnels With Polycarbonate Membrane Applications Part Number Description Pkg Concentrate organisms in aqueous FMFNL1050 Polycarbonate membrane, 0.4 µm, 100 mL, 50/pkg individually bagged, gamma-irradiated samples for further examination by PCR or eppifluorescence. FMFNL3020 Polycarbonate membrane, 0.4 µm, 300 mL, 20/pkg individually bagged, gamma-irradiated

Cell biology. MICROBIOLOGY QUALITY CONTROL Bioassays. Related Products Parasitology. Filter Funnel Manifolds ...... 228 - 229 Water microbiology. Sentino™ Microbiology Pump ...... 224 Stainless Steel Forceps ...... 230, 280 Vacuum/Pressure Pumps ...... 279

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 223 Sentino™ Microbiology Pump Maximize workspace and minimize contamination risk

Specifications

Materials of Construction Battery Housing Material: Polycarbonate ABS- Voltage: 12.0 V blended polymer Type: NiMH (nickel metal hydride) Keypad and Label: Polyester DC Supply Pump Head: PEI with PTFE-filled PBT Voltage: 24.0 V rollers Fluid Path Adaptor: Polypropylene AC/DC Power Transformer Hose Material: Thermoplastic elastomer 24 VDC 1.6 Amp 100-240 VAC, 47- 63 Hz Dimensions Input Interface: IEC 320 3-pin socket Length: 13.6 cm (5.4 in.) Output Interface (Power Entry Plug): Width: 9.5 cm (3.7 in.) 2.5 mm barrel jack Height: 10.4 cm (4.1 in.) Compact design frees valuable Weight: 680 g (1.5 lbs.) Power Cord benchtop space and provides Hose Dimensions: 4.8 mm (3/16 in.) ID 13180: IEC 320 connector and NEMA flexibility for an efficienct workflow. x 7.9 mm (5/16 in.), OD [1.6 mm 5-15P grounded plug (1/16 in.) wall thickness] 13181: IEC 320 connector and Minimal height of the pump allows European CEE 7/7 plug, and IEC 320 convenient use in a hood that can Temperature Range connector and UK BS1363 plug limit upward range of motion during Operation: 15-30 ºC (59-86 ºF) routine analysis. Storage: -15-45 ºC (5-113 ºF) Fluid path should be allowed to Pump’s disposable fluid path is equilibrate to operation range simple to load and completely before use disposable to eliminate potential biofilm build-up. Uni-directional peristaltic flow design pulls sample through the Ordering Information filter and fluid path eliminating the Sentino Microbiology Pump need for a vacuum source, and

filter funnels Part Number Description Pkg reducing potential for back-up 13180 System for North America: (1) power transformer, 1/pkg and contamination of analysis. (1) power cord with NEMA 5-15P plug, (1) Fluid Path, Fixed filtration parameters meet the and (1) User Guide CD Membrane Filter (MF) Technique 13181 System for locations outside North America: (1) power 1/pkg filtration parameters described in transformer, (1) European power cord with CEE 7/7 plug, U.S. EPA, ISO, and ASTM (1) UK power cord with BS1363 plug, (1) Fluid Path, and (1) User Guide CD standards. Shape of unit and materials of Accessories and Replacement Parts construction are easy to wipe down Part Number Description Pkg with standard laboratory 4861 Fluid Path, disposable, for use with MicroFunnel 10/pkg disinfectants. filter funnel Soft-touch keypad has simple on/off function and pulse features to Related Products control filtration. Ampoule Media for Microbiological Analysis ...... 217 MicroFunnel Filter Funnels ...... 219 - 223 Applications Petri Dishes...... 230, 280 Stainless Steel Forceps ...... 230, 280 MF Technique for analyzing aqueous-based fluids for microbial contamination. Works with MicroFunnel™ filter funnels. MICROBIOLOGY QUALITY CONTROL

224 37 mm Quality Monitors Compact and disposable filtration units for analysis of process water

Specifications

Materials of Construction Dimensions Filter Media: 0.45 µm GN-6 Metricel® Height: 2.8 cm (1.1 in.) (mixed cellulose esters), 0.2 µm Diameter: 4.2 cm (1.7 in.) Supor® (hydrophilic polyethersulfone) Operating Temperature and 0.45 µm Metricel Black (modified Room temperature, typically 20 - 25 ºC polyethersulfone) membranes (68 - 77 ºF) Monitor Inlet, Center Ring, and Outlet: Styrene Maximum Vacuum Plugs: Polyethylene 63.5 cm Hg (25 in. Hg) Absorbent Pad: Cellulose Etheylene Oxide Exposed Effective Filtration Area 9.1 cm2 Convenient, ready-to-use, and portable for easy transport to Ordering Information sample site. 37 mm Quality Monitors Saves time. Disposable design eliminates the cleaning and Part Number Description Pkg sterilization required of reusable 4717 2 piece, GN-6 Metricel membrane, white, gridded 50/pkg filter funnels. 4718 2 piece, Metricel Black membrane, gridded 50/pkg

4719 2 piece, 0.2 µm Supor membrane, white, gridded 50/pkg funnels filter Applications

Monitor high purity water systems for Related Products microbial contamination. Simple Ampoule Media for Microbiological Analysis ...... 217 design allows for sampling at the source or filtration in the lab. MICROBIOLOGY QUALITY CONTROL

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 225 Sentino™ Magnetic Filter Funnels Designed for use with the Sentino Microbiology Pump

Specifications

Materials of Construction Outlet Connection Funnel Body, Stem, Lid, and Support Stem fits into adapter (PN 4283) for use Screen: Polyphenylsulfone on Sentino Microbiology Pump. Vent Plugs: Polypropylene Sterilization Effective Filtration Area Provided non-sterile. Withstands 150 and 300 mL: 9.6 cm2, 35 mm multiple autoclavings* at effective diameter 121 - 123 °C (250 -253 °F) at 500 mL: 13.1 cm2, 41 mm 1.0 bar (100 kPa, 15 psi) for effective diameter 15 - 20 min; UV sterilizable.

Dimensions *Repeated use of detergents containing Overall Height: polyoxyethylated alkyl phenols and alcohols, 150 mL: 11.8 cm (4.6 in.) and/or anti-corrosion, anti- scaling boiler Available in three sizes – 150, 300, 300 mL: 16.8 cm (6.6 in.) additives that may carry over in steam, may and 500 mL. Convenient 150 mL 500 mL: 16.6 cm (6.5 in.) cause polyphenyl sulfone to crack, thereby size easily fits into small autoclaves; Maximum Diameter: reducing the life of the product. Do not autoclave rubber stoppers. Do not autoclave 500 mL size is ideal for large sample 150 and 300 mL: 7.6 cm (3 in.) filtration. with aluminum foil; use autoclave paper. 500 mL: 8.9 cm (3.5 in.) Consult Pall Technical Service if using with No-leak magnetic seal allows one- Filter Size thin membranes, such as track-etched handed operation. material. Accepts 47 mm filter Sturdy, safe polyphenylsulfone plastic construction is shatterproof Funnel Capacity and offers broad range of chemical 150, 300, or 500 mL compatibility. Membrane guides to help center Ordering Information filter on platform. Sentino Magnetic Filter Funnels, 47 mm Forceps access point allows easy Part Number Description Pkg filter funnels filter retrieval. 4271 150 mL capacity 1/pkg Graduated at 50 mL increments for 4273 300 mL capacity 1/pkg accurate sample measurement. 4277 500 mL capacity 1/pkg

Applications Accessories and Replacement Parts Part Number Description Pkg Use for the Membrane Filter (MF) 4279 Base with support screen for use with Sentino Pump 1/pkg Technique in: 4283 Adapter for use with Sentino Pump 1/pkg Drinking and waste water analysis 4285 Tubing, drive for Sentino Pump, 2 ft. length 10/pkg Environmental and recreational water 4235 Stainless steel support screen 1/pkg monitoring 87264 Support screen, polyphenylsulfone 1/pkg Pharmaceutical and industrial water system monitoring 4246 Lid kit (for 300 mL funnel only) 1/pkg 4248 150 mL funnel housing 1/pkg Beverage monitoring 4243 300 mL funnel housing 1/pkg Pharmaceutical raw materials, 4254 500 mL funnel housing 1/pkg intermediates, and final product.

Related Products Ampoule Media for Microbiological Analysis ...... 217 Petri Dishes...... 230, 280 Sentino Microbiology Pump ...... 224 Stainless Steel Forceps ...... 230, 280 MICROBIOLOGY QUALITY CONTROL

225a MICROBIOLOGY QUALITY CONTROL filter funnels 226 Applications liquids of filtration vacuum one-handed easy, allows seal magnetic Unique Funnels Filter Magnetic mm 47 large samples. large of filtration for ideal is size mL 500 and autoclaves, small into fit easy for allows size mL 150 Convenient, solvents. other many and agents anti-foaming with compatible is construction Polyphenylsulfone operation. handed one- allows seal magnetic No-leak accurate sample measurement. sample accurate for increments mL 50 at Graduated retrieval. filter easy allows point access Forceps funnels. glass most than less cost a at safety added and durability provides construction Polyphenylsulfone safe. and Sturdy Drinking water analysis. water Drinking testing. water process Plant analysis. water Surface testing. treatment water Municipal Technique. (MF) Filter Membrane the for Use Materials of Construction of Materials 500 mL: 13.1 cm 13.1 mL: 500 150, 300, or 500 mL 500 or 300, 150, Capacity Funnel filter mm 47 Accepts Size Filter Diameter: Maximum Height: Overall Dimensions cm 9.6 mL: 300 and 150 Area Filtration Effective PolypropyleneVentPlugs: Support Body,and Funnel Lid, Stem, Specifications Ordering Information Ordering atNme ecito Pkg 1/pkg 1/pkg 1/pkg 1/pkg 1/pkg Pkg screen support steel Stainless Description capacity mL 500 capacity,mL lid 300 with 4235 capacity mL 300 Number Part capacity mL 150 Parts Replacement and Accessories 4238 Description 4241 4242 4247 Number Part Funnels Filter Magnetic mm 47 22 o ubrsopr1/pkg 1/pkg 1/pkg 1/pkg 1/pkg 1/pkg 1/pkg stopper rubber No.8 housing funnel mL 500 housing funnel mL 300 housing funnel mL 150 only) funnel mL 300 (for kit Lid screen support Base,without 82728 4254 screen,polyphenylsulfone Support 4243 4248 4246 4244 87264 aumPesr up . . Pumps Vacuum/Pressure . . Forceps Steel Stainless . . Manifolds Funnel Filter . . Microbiological Analysis for Media Ampoule Products Related effective diameter effective 500 mL: 8.9 cm (3.5 in.) (3.5 cm 8.9 mL: 500 in.) (3 cm 7.6 mL: 300 and 150 in.) (7.7 cm 19.6 mL: 500 in.) (9 cm 22.9 mL: 300 in.) (7.0 cm 17.8 mL: 150 Polyphenylsulfone Screen: effective diameter effective 2 , 41 mm 41 , 2 , 35 mm 35 , *Repeated use of detergents con detergents of use *Repeated sterilizable. UV min; 20 - 15 for psi) 15 kPa, (100 bar 1.0 at °F) -253 (250 °C 123 - 121 at autoclavings* multiple Withstands non-sterile. Provided Sterilization stopper. one-hole standard into fits Stem Connection Outlet auto not Do product. the of life the reducing polyphenyl steam,carryoverincausemay anti- anti-corrosion, and/or alcohols, and phenols alkyl polyoxyethylated such as track-etched material. track-etched as such membranes, thin with using if Service paper.Technicalautoclave Pall Consult auto clave rubber stoppers. Do not Do stoppers. rubber clave clave with aluminum foil; use foil; aluminum with clave scaling boiler additives that may that additives boiler scaling sulfone to crack, thereby crack, to sulfone ...... 228 - 229 - . 228 . 230,280 . 279 .217 taining Microcheck® II Beverage Monitors Easy-to-use disposable filter funnel to meet microbial analysis needs for beverages

Specifications

Materials of construction Dimensions: Filter Media: GN Metricel® (mixed Height: 7.6 cm (3.0 in.) cellulose esters) and Metricel Diameter: 6.1 cm (2.4 in.) Black (modified polyethersulfone) Packaging Note membranes Two layers of 25 funnels bulk packaged Cylinder and Base: Polypropylene within one box-liner bag. Cover: Polystyrene Plug: Polyethylene Gamma-Irradiated Funnel Adapter: Polyethylene Support Pad: Cellulose

Ordering Information Can convert to a Petri dish for Microcheck II Beverage Monitors culturing or easily remove the membrane for placing on separate Part Number Description Pkg agar dish. 4761 0.45 µm, GN-6 Metricel membrane, white, 50/pkg gridded, 100 mL capacity Ready to use; simply remove from 4762 0.8 µm, GN-4 Metricel membrane, white, 50/pkg box and place directly on a filter gridded, 100 mL capacity manifold. No need for rubber 4763 0.45 µm, Metricel black membrane, black, 50/pkg

stoppers or adapters. funnels filter gridded, 100 mL capacity Volume graduations are marked 4764 0.8 µm Metricel black membrane, black, 50/pkg clearly for easy reading from any gridded, 100 mL capacity point of view. Volume graduations marked externally on the funnel to prevent potential Related Products hold-up of organisms. External marks Ampoule Media for Microbiological Analysis ...... 217 meet guidelines listed in Standard Filter Funnel Manifolds ...... 228 - 229 Methods for the Analysis of Water Petri Dishes...... 230, 280 and Wastewater, current edition. Stainless Steel Forceps ...... 230, 280 MICROBIOLOGY QUALITY CONTROL

Applications

Finished product and raw material testing of beverages for microbial contamination prior to release. Available with 0.45 and 0.8 µm, white or black membranes, to easily quantify organisms.

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 227 MICROBIOLOGY QUALITY CONTROL hardware 228 Applications funnel filter MicroFunnel the with use for manifolds vacuum fit Perfect Funnels Filter MicroFunnel™ for Manifolds Funnel Filter up and compatibility with many with compatibility and up clean- easy for construction steel stainless and aluminum Durable sizes. mL 300 and mL 100 the including funnels, filter MicroFunnel Sciences Life Pall all with Works place. in funnel filter the hold requiredto stoppers rubber or adapters No of the way.the of out store to easy and offsite, or lab a around moving for portable easily is manifold Single-place space. counter large for need the reduces that footprint small a has manifold Single-place chemicals. each day.each tested are samples of numbers minimal or infrequent when manifold 1-place the use or simultaneously samples multiple Process filtration. begin to on turned is vacuum the and added, is liquid the manifold, the onto directly placed is funnel filter The analysis. microbial TechniqueMF the for performing when funnel filter MicroFunnel the with work to Designed Materials of Construction of Materials Manifold 1-Place 15 psi) for 15 - 20 min. 20 - 15 for psi) 15 kPa, (100 bar 1.0 at ºF) 253 - (250 ºC 123 - 121 at desired if Autoclave Sterilization Barb): Hose (Without Diameter in.) (2.4 cm 5.9 Height: Dimensions steel Stainless Adapter: Barb Hose with steel stainless Valve:316 Check aluminum Anodized Body: Petri Dishes. . . Dishes. Petri . . Funnels Filter MicroFunnel . . Microbiological Analysis for Media Ampoule Products Related Specifications Ordering Information Ordering atNme ecito Pkg 1/pkg 1/pkg 1/pkg Pkg Description aluminum 6-place aluminum 3-place aluminum 1-place Number Part Manifold) (Aluminum Parts Replacement and Accessories Description 15413 15411 15408 Number Part Funnels Filter MicroFunnel for Manifolds Funnel Filter 93 tils te n lg1/pkg 1/pkg 1/pkg 1/pkg 1/pkg 3/pkg 1/pkg manifolds aluminum 6-place or 3- for valve Check valves,O-rings,includes:for 3 kit O-ring plug end steel Stainless adapter,barb Hose in.nylon,MNPT 1/4 15415 Valve,2-way stand End 88160 holder Funnel 99238 99132 99130 96430 15412 7.5 cm (3.0 in.) (3.0 cm 7.5 MNPT in. 1/8 straight, in. 1/4 O-ring propylene ethylene ARP No. -006; 6 O-rings,6 No.No. -010 -006; ARP ARP tubing ID in.) (3/8 mm 9.5 to 6-Place Manifold 6-Place in.) (6.0 cm 15.2 Depth: in.) (16.0 cm 40.6 Width: in.) (5.6 cm 14.2 Height: Manifold 3-Place Dimensions mm 6.4 Nylon Adapter: Barb Hose Viton ValveO-Rings: Valves:brass Chrome-plated with steel stainless Valve:316 Check MNPT in. 1/4 steel Stainless Plug: Drain aluminum Anodized Body: Construction of Materials Manifolds 6-Place and 3- 15 psi) for 15 - 20 min. 20 - 15 for psi) 15 kPa, (100 bar 1.0 at ºF) 253 - (250 ºC 123 - 121 at desired if Autoclave Sterilization in.) (6.0 cm 15.2 Depth: in.) (32.5 cm 82.6 Width: in.) (5.6 cm 14.2 Height: (1/4 in.) (1/4 o-ring propylene ethylene ◆ .230,280 ...... 219 - 223 - .219 .217 Filter Funnel Manifolds The most convenient way to filter multiple samples

Specifications

Aluminum Manifolds Sterilization Materials of Construction Autoclavable if desired at 121 - 123 °C Body: Anodized aluminum (250 - 253 °F) at 1.0 bar (100 kPa, Drain Plugs: Stainless steel 15 psi) for 15 - 20 min. 1/4 in. MNPT Valves: Chrome-plated brass Polyurethane Manifold Valve O-Rings: Viton◆ Materials of Construction Adapter: Nylon 6.4 mm (1/4 in.) Body: High impact polyurethane Drain Plugs: Nylon 1/4 in. MNPT Dimensions Valves: Glass-filled polypropylene 3-place Valve O-Rings: Buna◆-N Height: 15.7 cm (6.2 in.) Width: 40.6 cm (16.0 in.) Dimensions Depth: 15.2 cm (6.0 in.) Height: 10.2 cm (4.0 in.) Independent operation. Each Width: 27.9 cm (11.0 in.) funnel location has individual port 6-place Depth: 15.2 cm (6.0 in.) control valves. Height: 15.7 cm (6.2 in.) Lightweight and durable for Width: 82.6 cm (32.5 in.) easy handling. Depth: 15.2 cm (6.0 in.) Saves money. Less costly than stainless steel filter funnel manifolds. Ordering Information Large port opening makes Filter Funnel Manifolds sanitizing easy. Part Number Description Pkg hardware Versatile. Accommodates both 4205 3-place polyurethane 1/pkg 25 and 47 mm filter funnels. 15402 3-place aluminum 1/pkg Aluminum manifolds available with 15403 6-place aluminum 1/pkg either 3 or 6 places. Accessories and Replacement Parts (Aluminum Manifold) Applications Part Number Description Pkg 96429 Funnel holder 1/pkg MICROBIOLOGY QUALITY CONTROL Designed for use in the vacuum 96430 End stand 1/pkg filtration of liquids for analysis of 99130 Valve, 2-way 1/pkg microbiological or particulate 99132 Hose barb adapter, nylon, 1/4 in. MNPT 1/pkg contamination. Increase laboratory to 9.5 mm (3/8 in.) ID tubing productivity by processing multiple 99238 Stainless steel end plug 1/pkg samples simultaneously. 82728 No. 8 rubber stopper 1/pkg Polyurethane manifold is ideal for 88160 O-ring kit for valves, includes: 3 O-rings, 1/pkg small work areas. Only 27.9 cm ARP No. -006; 6 O-rings, ARP No. -010 (11.0 in.) wide, it still holds three 15415 Check valve for 3- or 6-place aluminum manifolds 3/pkg funnels. Aluminum manifold is especially Accessories and Replacement Parts (Polyurethane Manifold) suited for applications where Part Number Description Pkg chemical compatibility is critical and 81308 No. 2 stopper for manifold 1/pkg easy clean-up is desired. Lightweight 39961 Manifold rebuild kit, includes 3 stainless steel 1/pkg aluminum is easier to handle and retaining rings; 6 O-rings, ARP No. -016; 3 O-rings, less expensive than stainless steel, ARP No. -015; 6 O-rings, ARP No. 009; 1 1/4 in. while retaining the strength of MNPT plug; 2 1/4 in. MNPT to 6.4 mm (1/4 in.) hose an alloy. barb adapters; 4 foot pads; and 1 each knob and valve

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 229 MICROBIOLOGY QUALITY CONTROL accessories 230 Unique patented design for easy handling and storage and handling easy for design patented Unique Dishes Petri easy handling filter Make Forceps Steel Stainless and see on the lab bench. lab the on see and identify,to easy track, forceps make colors Bright grips. finger colored multi- or black traditional Choose hold. secure and comfortable a provide grips finger Polypropylene damage. filter membrane avoid to surface smooth flat, a have Tips Bulk packaging offers additional value. additional offers packaging Bulk pads. absorbent without or with Available growth.microbial impede to residuals EtO No Gamma-irradiated. base. stacking easy with incubator the in or bench lab the on space less Uses seal. tight a to closes yet hand, one with easily Opens Applications Applications Material of Construction of Material Dish Petri Construction of Materials Accepts 47 mm membrane filter membrane mm 47 Accepts Size Filter in.) (1.97 mm 50.0 Diameter: in.) (0.35 mm 9.0 Height: Dimensions Polystyrene grips finger polypropylene with steel Stainless Specifications Specifications Ordering Information Ordering Information Ordering atNme ecito Pkg 1/pkg 3/pkg grips forceps,multi-colored steel Stainless grips forceps,black steel Stainless Description 4690 51147 Number Part Forceps Steel Stainless 25Ptidse,wt bobn as100/pkg Pkg 100/pkg 500/pkg pads absorbent dishes,with Petri pads absorbent pack,dishes,without bulk Petri pads absorbent dishes,without Petri Description 7245 7232 7242 Number Part Dishes Petri (MF) Technique.(MF) Filter Membrane the performing when analysis microbiological for Ideal dishes. Petri and holders filter from and to membrane moving and handling for Ideal (1 each of orange,blue,of chartreuse) each (1 in aluminum foil. aluminum in autoclave not Do min. 20 - 15 for psi) 15 kPa, (100 bar 1.0 at ºF) 253 - (250 use to prior tips the "Flame" non-sterile. Provided Sterilization 0.9 mm (35 mils) (35 mm 0.9 TypicalThickness Cellulose Composition Pads Absorbent kGy 30 - 15 Validateddose Gamma-Irradiated absorbent pads. absorbent without dishes into agar pour can users or media, broth with used be can pads absorbent with dishes Petri , or autoclave at 121 - 123 ºC 123 - 121 at autoclave or , Absorbent Pad Kits One-handed dispensing of cellulose absorbent pads

Specifications

Pad Composition Typical Thickness Cellulose 0.9 mm (35 mils) Diameter 45.5 mm

Ordering Information Absorbent Pad Kits Part Number Description Pkg 66025 Absorbent pads, gamma-irradiated 1,000/pkg 66190 Absorbent pads, non-sterile 1,000/pkg

Enables user to dispense a clean cellulose pad into a Petri dish Related Products whenever needed without touching 47 mm Membrane Disc Filters ...... 213 - 215 the pad. Ampoule Media for Microbiological Analysis ...... 217 Handy dispenser kit holds 1 tube of Petri Dishes...... 230, 280 100 absorbent pads (10 tubes included). Each tube drops quickly into the hand dispenser for easy use. accessories Available non-sterile or gamma- irradiated. No EtO residuals to impede microbial growth.

Applications

Absorbent pads are ideal for absorbing broth media to culture colonies in accordance with the Membrane Filter (MF) Technique. MICROBIOLOGY QUALITY CONTROL

1. 8 0 0 .5 2 1 . 1 5 2 0 ( U S A and Canada) (+)800.PALL.LIFE (Outside USA and Canada) www.pall.com/lab 231