Chemistry Lab Kit Instructions
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Standard Operating Procedure SEA05 Laboratory Analysis-Version
Gulf Coast Network National Park Service Inventory and Monitoring Division Standard Operating Procedure SEA05 Laboratory Analysis—Version 1.0 Please cite this as: Meiman, J. 2019. Laboratory Analysis—Version 1.0. Gulf Coast Network Standard Operating Procedure NPS/GULN/SOP—SEA05. Gulf Coast Network, Lafayette, Louisiana. Summary This standard operating procedure (SOP) for laboratory analysis of total suspended solids (TSS) is adopted verbatim from USEPA Method 160.2. Revision Log Revision Date Author Changes Made Reason for Change New Version # METHOD #: 160.2 Approved for NPDES (Issued 1971) TITLE: Residue, Non-Filterable (Gravimetric, Dried at 103–105°C) ANALYTE: Residue, Non-Filterable INSTRUMENTATION: Drying Oven STORET No. 00530 1. Scope and Application a. This method is applicable to drinking, surface, and saline waters, domestic and industrial wastes. b. The practical range of the determination is 4 mg / L to 20,000 mg / L. 2. Summary of Method a. A well-mixed sample is filtered through a glass fiber filter, and the residue retained on the filter is dried to constant weight at 103-105°C. b. The filtrate from this method may be used for Residue, Filterable. 3. Definitions a. Residue, non-filterable, is defined as those solids which are retained by a glass fiber filter and dried to constant weight at 103-105°C. 4. Sample Handling and Preservation a. Non-representative particulates such as leaves, sticks, fish, and lumps of fecal matter should be excluded from the sample if it is determined that their inclusion is not desired in the final result. b. Preservation of the sample is not practical; analysis should begin as soon as possible. -
ARC Laboratory Manual for Architectural Conservators
A LABORATORY MANUAL FOR ARCHITECTURAL CONSERVATORS Jeanne Marie Teutonico ICCROM Rome 1988 CONTENTS Foreword v Preface vii General Principles: Laboratory Science 1. Sampling 3 2. Measurement and Error: Precision, Accuracy, Statistics 7 3. Measurement: Mass (Use of the Balance) 11 4. Measurement: Length (Use of the Vernier Caliper and Micrometer) 16 5. Measurement: Volume 21 6. Measurement: Solutions 26 7. Measurement: pH 30 Porous Building Materials 8. Water Absorption by Total Immersion 35 9. Water Drop Absorption 41 10. Penetration of Water: Capillary Action 43 11. Porosity of Granular Beds 45 12. Porosity in Solids: Indirect Measurement by Water Absorption 50 13. Porosity in Solids: Hydrostatic Weighing 52 14. Movement of Salts 56 15. Salt Crystallization 57 16. Qualitative Analysis of Water-soluble Salts and This publication was printed with a generous Carbonates 58 contribution from the government of 17. Semiquantititive Analysis of Water-soluble Salts 68 Finland. Earthen Building Materials 18A. Particle Size Analysis: Part I Sieving Procedure 73 ISBN 92-9077-083-X Via di San Michele 13 00153 Rome RM, Italy Printed in Italy III Earthen Building Materials (continued) FOREWORD 18B. Particle Size Analysis: Part II Sedimentation Procedure: Hydrometer Method 83 19. Plastic Limit of Soils 96 After his appointment as director of ICCROM in 1977, Sir Bernard FEILDEN proposed to emphasize the interdisciplinary 20. Liquid Limit of Soils 102 character of conservation by introducing scientists to field work and architects to laboratories. Consequently it was decided to introduce a series of laboratory exercises Stone. Brick and Mortars designed for the needs of architectural conservators and conservation architects in the program of the International 21. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Chemistry M11 Laboratory Manual
Chemistry M11 Laboratory Manual Laboratory Experiments for General, Organic, and Biochemistry Compiled by the Department of Chemistry at Moorpark College Version 2.0 Fall 2020 – Present NH2 O O CN NH2 N N Co The Vitamin B molecule shown is useful in the treatment of N N 12 H2N pernicious anemia and other diseases. Enzymes derived from O O vitamin B12 accelerate a large range of important reactions NH2 including those involved in producing red blood cells. HN O N HO N O O P O O O CH2OH Moorpark College Department of Chemistry Chemistry 11 Lab Manual Table of Contents Laboratory Experiments Page Number Experiment 1 – Separation of Copper(II) Sulfate from Sand ............................................. 3 Experiment 2 – Measurements............................................................................................ 6 Experiment 3 – Properties of Solutions ............................................................................ 13 Experiment 4 – Double Displacement Reactions ............................................................. 20 Experiment 5 – Single Displacement Reactions ............................................................... 25 Experiment 6 – Precipitation of Strontium Sulfate ........................................................... 29 Experiment 7 – Ionization and the Nature of Acids, Bases, and Salts .............................. 33 Experiment 8 – Acid/Base Titrations ................................................................................ 42 Experiment 9 – Structure in Inorganic & Organic -
SILICA, CRYSTALLINE in Coal Mine Dust, by IR 7603
SILICA, CRYSTALLINE in coal mine dust, by IR 7603 SiO2 MW: 60.08 CAS: 14808-60-7 RTECS: VV7330000 METHOD: 7603, Issue 2 EVALUATION: UNRATED Issue 1: 15 May 1989 Issue 2: 15 August 1994 quartz (respirable): 3 OSHA : 10 mg/m /(%SiO2 + 2) PROPERTIES: solid; crystalline transformations: quartz NIOSH: 0.05 mg/m3 (suspect carcinogen) to tridymite @ 867 °C: tridymite to ACGIH: 0.1 mg/m3 crystobalite @ 1470 °C; a-quartz to b-quartz @ 573 °C SYNONYMS: free crystalline silica; silicon dioxide SAMPLING MEASUREMENT SAMPLER: CYCLONE + PREWEIGHED FILTER TECHNIQUE: INFRARED SPECTROPHOTOMETRY (IR) (10-mm cyclone, nylon, or Higgins-Dewell (HD), and PVC filter, 37-mm, 5-µm) ANALYTE: quartz FLOW RATE: HD cyclone: 2.2 L/min WEIGH: dust cassette nylon cyclone: 1.7 L/min ASH: muffle furnace or RF plasma asher VOL-MIN: 300 L @ 0.1 mg/m 3 -MAX: 1000 L REDEPOSIT: 0.45-µm acrylic copolymer membrane filter SHIPMENT: routine IR: scan, 1000 to 650 cm -1, absorbance mode SAMPLE STABILITY: stable with blank filter in reference beam BLANKS: 2 to 10 field blanks per set CALIBRATION: standard suspension of quartz in 2-propanol BULK SAMPLE: required for OSHA standard calculations; area respirable or settled dust RANGE: 30 to 250 µg quartz per sample [1] ESTIMATED LOD: 10 µg quartz per sample [1] ACCURACY PRECISION (S r): 0.098 @ 100 to 500 µg per sample RANGE STUDIED: 25 to 160 µg/sample [1] (varies with sample matrix) [1] (2 mg quartz/m 3 atmosphere) BIAS: unknown ˆ OVERALL PRECISION (S rT): 0.13 to 0.22 (varies with sample loading and matrix) ACCURACY: ±25.6 to 43.4% APPLICABILITY: The working range is 0.03 to 2 mg/m 3 for a 1000-L sample. -
SILICA, CRYSTALLINE, by XRD (Filter Redeposition) 7500
SILICA, CRYSTALLINE, by XRD (filter redeposition) 7500 SiO2 MW: 60.08 CAS: 14808-60-7 (quartz) RTECS: VV7330000 (quartz) 14464-46-1 (cristobalite) VV7325000 (cristobalite) 15468-32-3 (tridymite) VV7335000 (tridymite) METHOD: 7500, Issue 4 EVALUATION: FULL Issue 1: 15 August 1990 Issue 4: 15 March 2003 3 3 OSHA : quartz (respirable) 10 mg/m /(%SiO2+2); PROPERTIES: solid; d 2.65 g/cm @ 0 °C; crystalline cristobalite and tridymite (respirable) ½ the above transformations: quartz to tridymite NIOSH: 0.05 mg/m3; carcinogen @ 867 °C; tridymite to cristobalite ACGIH: quartz (respirable) 0.1 mg/m3 @ 1470 °C; "-quartz to ß-quartz cristobalite (respirable) 0.05 mg/m3 @ 573 °C tridymite (respirable) 0.05 mg/m3 SYNONYMS: free crystalline silica; silicon dioxide SAMPLING MEASUREMENT SAMPLER: CYCLONE + FILTER TECHNIQUE: X-RAY POWDER DIFFRACTION (10-mm nylon cyclone, Higgins- Dewell (HD) cyclone, or aluminum ANALYTE: Crystalline SiO2 cyclone + 5-:m PVC membrane) *see sampling section ASH: Muffle furnace or RF plasma asher or dissolve in tetrahydrofuran FLOW RATE: Nylon cyclone: 1.7 L/min; HD cyclone: 2.2 L/min; REDEPOSIT: On 0.45-:m Ag membrane filter aluminum cyclone: 2.5 L/min XRD: Cu target X-ray tube, graphite VOL-MIN: 400 L monochromator -MAX: 1000 L Optimize for intensity; 1° slit Slow step scan, 0.02°/10 sec SHIPMENT: Routine Integrated intensity with background subtraction SAMPLE STABILITY: Stable CALIBRATION: :NIST SRM 1878a quartz, NIST SRM 1879a cristobalite, USGS 210-75-0043 BLANKS: 2 to 10 per set (see step 13.g.) tridymite suspensions in 2-propanol. BULK SAMPLE: High-volume or settled dust; to RANGE: 0.02 to 2 mg SiO2 per sample [2] identify interferences ESTIMATED LOD: 0.005 mg SiO2 per sample [2] ACCURACY þ PRECISION ( r): 0.08 @ 0.05 to 0.2 mg per sample [1] RANGE STUDIED: 25 to 2500 :g/m3 [1] (800-L sample) BIAS: None known Ö : OVERALL PRECISION ( rT): 0.09 (50 to 200 g) [1] ACCURACY: ± 18% APPLICABILITY: The working range is 0.025 to 2.5 mg/m3 for an 800-L air sample. -
Complete Manual!
EXPERIMENT MANUAL Please observe the safety information, first aid information, information about hazardous substances, and poison control information on the inside front cover; the advice for supervising adults on page 3; and the safety rules on page 4. WARNING. WARNING — Chemistry Set. Not suitable for children This set contains chemicals and/ under 6 years. For use or parts that may be harmful under adult supervision. if misused. Read cautions on Read the instructions individual containers and in manual before use, follow them and carefully. Not to be used by children keep them for reference. except under adult supervision. Franckh-Kosmos Verlags-GmbH & Co. KG, Pfizerstr. 5-7, 70184 Stuttgart, Germany | +49 (0) 711 2191-0 | www.kosmos.de Thames & Kosmos, 301 Friendship St., Providence, RI, 02903, USA | 1-800-587-2872 | www.thamesandkosmos.com › › › SAFETY INFORMATION Safety Information ››› WARNING. Not suitable for children under 3 years. Choking Poison Control Centers hazard — small parts may be swallowed or inhaled. (United States) ››› Keep the packaging and instructions as they contain important information. In case of emergency, your nearest poison First Aid Information control center can be ››› In case of eye contact: Wash out eye with plenty of water, reached everywhere in holding eye open. Seek immediate medical advice. the United States by ››› If swallowed: Wash out mouth with water, drink some fresh dialing the number: water. Do not induce vomiting. Seek immediate medical advice. ››› In case of inhalation: Remove person to fresh air. ››› In case of skin contact and burns: Wash affected area with 1-800-222-1222 plenty of water for at least 10 minutes. -
Edna PROTOCOL SAMPLE COLLECTION
eDNA PROTOCOL SAMPLE COLLECTION Caren Goldberg and Katherine Strickler, Washington State University September 2014 Adapted from Protocol Version 04/12/2012 (D.S. Pilliod, R.S. Arkle, and M.B. Laramie) USGS Snake River Field Station MATERIALS 1. Cellulose nitrate disposable filter funnels or other field-tested, disposable filter funnels 2. Vacuum flask (1L) 3. Silicone tubing 4. Vacuum hand pump (from auto parts store) 5. Rubber stopper with hole for funnel stem 6. Latex or nitrile gloves (non-powdered) 7. Forceps (filter forceps if possible) 8. High quality, o-ring screw cap 2mL tubes (e.g., Sarstedt brand) with 1mL 100% molecular-grade ethanol (not denatured) 9. Ethanol-proof laboratory pen (do not use a regular Sharpie marker) 10. 50 mL tubes of 30 mL 1) 50% household bleach solution 2) distilled water in a holder (a foam drink holder [koozie] works well) 11. Polypropylene grab bottles and cooler (for off-site filtering) or Whirl-Pak® bags (for on- site filtering) 12. Water, bleach, scrub brush, and tubs (for decontaminating between sites) 2 3 5 1 4 2 Figure 1. Filter funnel (1), vacuum flask (2), silicone tubing (3), vacuum pump (4), and rubber stopper (5). 9 7 10 8 6 1 Figure 2. Latex or nitrile gloves (6), forceps (7), 2 mL tubes with 1 mL ethanol (8), ethanol-proof lab marker (9), and 2 50 mL tubes with 50% household bleach solution distilled water (10). 3 11a 11b Figure 3. Polypropylene grab bottles (11a) and Whirl-Pak® bag (11b). 12a 12d 12b 12c Figure 4. Water (12a), bleach (12b), scrub brushes (12c), and tubs (12d) for decontaminating boots and equipment between sites. -
CHEM C3000 Manual
EXPERIMENT MANUAL Version 2.0 Please observe the safety information, the advice for supervising adults on page 5, the safety rules on page 6, the information about hazardous substances and mixtures (chemicals) on pages 7-9 and their environmentally sound disposal on pages 175-177, the safety for experiments with batteries on page 192, the first aid information on the inside front cover and the instructions on the use of the alcohol burner on page 12. WARNING. Not suitable for children under 12 years. For use under adult supervision. Contains some chemicals which present a hazard to health. Read the instructions before use, follow them and keep them for reference. Do not allow chemicals to come into contact with any part of the body, particularly the mouth and eyes. Keep small children and animals away from experiments. Keep the experimental set out of the reach of children under 12 years old. Eye protection for supervising adults is not included. WARNING — Chemistry Set. This set contains chemicals and parts that may be harmful if misused. Read cautions on individual containers and in manual carefully. Not to be used by children except under adult supervision. Franckh-Kosmos Verlags-GmbH & Co. KG, Pfizerstr. 5-7, 70184 Stuttgart, Germany | +49 (0) 711 2191-0 | www.kosmos.de Thames & Kosmos, 301 Friendship St., Providence, RI, 02903, USA | 1-800-587-2872 | www.thamesandkosmos.com Thames & Kosmos UK Ltd, Goudhurst, Kent, TN17 2QZ , United Kingdom | 01580 212000 | www.thamesandkosmos.co.uk Contents Safety and Information First Aid Information . Inside front cover Poison Control Contact Information . Inside front cover Advice for Supervising Adults . -
Laboratory Filtration Principles and Chemical Compatibility Chart Filtration Simplified
Laboratory filtration Principles and chemical compatibility chart Filtration simplified Basic filtration concepts and terms Selecting a filter with the appropriate properties can help you achieve accurate results and reach discovery faster. But with so many types of filters to choose from, how can you be sure you’re making the right choice? GE Healthcare Life Sciences, maker of Whatman™ brand filtration products, has assembled this compilation of basic filtration concepts and terms to clarify the various options available to you and speed up your selection process. Ash content Hydrophilic Determined by ignition of the cellulose filter at 900°C Because hydrophilic filters possess an affinity for water in air. Minimizing ash content is essential in gravimetric and can be wetted with virtually any liquid, they are applications and also a useful measure of the level of typically used for aqueous solutions and compatible general purity. organic solvents. Chemical compatibility Hydrophobic It is very important to ensure that the structure of the These types of filters repel water, and are thus best suited filter media will not be impaired by exposure to certain for filtering organic solvents as well as for venting and chemicals. In addition, exposure to these chemicals gas filtration applications. should not cause the filter to shed fibers or particles, or add extractables. Length of exposure time, temperature, Liquid flow rate (including Herzberg method) concentration and applied pressure can all affect Under practical filtration conditions, the liquid flow rate compatibility. GE Healthcare has provided chemical will depend on a number of factors, many of which will compatibility charts to aid your filter selection. -
CHE 131 Chemistry Set Inventory List 1 Digital Scale 1 Digital
CHE 131 Chemistry Set Inventory List 1 Digital Scale 1 Digital Thermometer 100 mL Graduated Cylinder 3 Mixing Containers with Screw Lids (100 mL) 1 Screw Lid Test Tube (50 mL size) 10 Screw Lid Test Tubes (15 mL size) 100 mL Volumetric Flask 4 Large Plastic Container (1 quart) 4 Lids for Large Plastic Container (1 quart) 1 Mortar and Pestle 3 Droppers 1 Permanent Marker 1 10× Magnifying Loupe 1 Ruler 1.0 mL Syringe 1 Small Metal Binder Clip 1 Weighing Boat 1 Platonic Solid 1 Aluminum Pan 13 Disposable Filter Funnels 13 Disposable Filters 16 Large Dixie Cups 2 Small Dixie Cups 1 Plastic Squirt Bottle 4 Inch Framing Nail 1 Plastic Spoon 1 LED Flashlight 3 AAA Batteries 1 Blue Light Filter 11 Square Plastic Cuvettes (4.5 mL) 2 Squares (1.0 inch) of Double Stick Tape 1 Conductivity Apparatus consisting of: - Conductivity Sensor/Electrode - 12 Volt Green LED - 3 Volt Power Supply 1 Microfiber Rag Packet of Blue Litmus Paper Packet of Red Litmus Paper 2 Bottles Deionized Water 1 Large Dixie Cup Full of Lego Pieces 1 Gray Lego Board 1 Roll of Duct Tape 1.0 mL of Brilliant Blue FCF (Blue FC in an Eppendorf Tube) 15 mL of 1.0 % Acetic Acid (in a 15 mL Screw Lid Test Tube) 2.0 grams of L-Arginine 1 Baggie Full of Citric Acid 1 Baggie Full of Magnesium Sulfate Heptahydrate 1 Baggie Full of Sodium Chloride 1 Baggie Full of Sucrose 1 Baggie Full of Calcium Chloride Dihydrate 1 Baggie Full of Urea Calcium Sulfate Packet containing: 1) Materials Safety Data Sheets for all enclosed chemicals 2) Copies of all experimental laboratory procedures CHE 132 -
Environmental DNA Sampling Protocol—Filtering Water to Capture DNA from Aquatic Organisms
Prepared in cooperation with Washington State University Environmental DNA Sampling Protocol—Filtering Water to Capture DNA from Aquatic Organisms Chapter 13 of Section A, Biological Science Book 2, Collection of Environmental Data Techniques and Methods 2–A13 U.S. Department of the Interior U.S. Geological Survey Cover: Okanogan River, British Columbia, Canada, 2012. Insets from top to bottom: Juvenile rainbow trout from Ratz Creek, Alaska, 2009. (C)2009 ADF&G, Division of Sport Fish. Used with permission. Collecting eDNA sample from Boise River, Boise, Idaho, 2011. USGS Technician Kevin Glueckert collects eDNA sample from Boise River, Boise, Idaho, 2014. Preserving folded eDNA sample in ethanol vial-filled vial, 2012. Photographs by Matthew B. Laramie, U.S. Geological Survey, unless otherwise noted. Environmental DNA Sampling Protocol— Filtering Water to Capture DNA from Aquatic Organisms By Matthew B. Laramie, David S. Pilliod, Caren S. Goldberg, and Katherine M. Strickler Chapter 13 of Section A, Biological Science Book 2, Collection of Environmental Data Prepared in cooperation with Washington State University Techniques and Methods 2–A13 U.S. Department of the Interior U.S. Geological Survey U.S. Department of the Interior SALLY JEWELL, Secretary U.S. Geological Survey Suzette M. Kimball, Acting Director U.S. Geological Survey, Reston, Virginia: 2015 For more information on the USGS—the Federal source for science about the Earth, its natural and living resources, natural hazards, and the environment—visit http://www.usgs.gov or call 1–888–ASK–USGS. For an overview of USGS information products, including maps, imagery, and publications, visit http://www.usgs.gov/pubprod/.