Advance Publication by J-STAGE Japanese Journal of Infectious Diseases Poliovirus detection and genetic characteristic from sewage in Heilongjiang province from 2013 to 2016 Yujie Ma, Xiaoyu Wang, Xue Zhou, Po Lv, Han Wang, Jing Song, Changjiang Song, and Zhaodan Sun Received: July 27, 2017. Accepted: June 28, 2018 Published online: July 31, 2018 DOI:10.7883/yoken.JJID.2017.338 Advance Publication articles have been accepted by JJID but have not been copyedited or formatted for publication. Poliovirus detection and genetic characteristic from sewage in Heilongjiang province from 2013 to 2016 Yujie Ma*, Xiaoyu Wang, Xue Zhou, Po Lv, Han Wang, Jing Song, Changjiang Song, Zhaodan Sun, Expanded Program on Immunization, Heilongjiang Provincial Center for Disease Control and Prevention, Harbin Corresponding author: Mailing address: Institute of Planned Immunity, Heilongjiang Center for Disease Control and Prevention, 40 Youfang Street, Xiangfang District, Harbin, Heilongjiang Province, China, 150030. Tel&Fax: +86-451-55153604, E-mail:
[email protected] Key words: Poliovirus, Environmental Surveillance, VP1, Genetic Mutation Running Title: Poliovirus detection and genetic characteristic analysis Accepted Manuscript Summary: By monitoring sewage in Heilongjiang Province from 2013 to 2016, in this research we analyzed the epidemiological tendency and genetic mutation found in the poliovirus in the environment in order to setup a warning system for vaccine-derived poliovirus (VDPV) and spread of wild poliovirus. In this study, we collected 139 sewage samples from eight regions in Heilongjiang Province. Poliovirus was identified from 72 samples and the positive rate was 51%. 263 strains of poliovirus were isolated, including 22 type1, 104 type2 and 137 type3 polioviruses.