VPS25 (1-176, His-Tag) Human Protein – AR50272PU-S | Origene

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VPS25 (1-176, His-Tag) Human Protein – AR50272PU-S | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for AR50272PU-S VPS25 (1-176, His-tag) Human Protein Product data: Product Type: Recombinant Proteins Description: VPS25 (1-176, His-tag) human recombinant protein, 0.1 mg Species: Human Expression Host: E. coli Tag: His-tag Predicted MW: 23.3 kDa Concentration: lot specific Purity: >85% by SDS - PAGE Buffer: Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M Nacl Preparation: Liquid purified protein Protein Description: Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Stability: Shelf life: one year from despatch. RefSeq: NP_115729 Locus ID: 84313 UniProt ID: Q9BRG1, A0A024R1X3 Cytogenetics: 17q21.2 Synonyms: DERP9; EAP20; FAP20 Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013] Protein Families: Transcription Factors This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 VPS25 (1-176, His-tag) Human Protein – AR50272PU-S Protein Pathways: Endocytosis Product images: This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Genetic and Genomic Analysis of Hyperlipidemia, Obesity and Diabetes Using (C57BL/6J × TALLYHO/Jngj) F2 Mice
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Product Information
Product information VPS25, 1-176aa Human, His-tagged, Recombinant, E.coli Cat. No. IBATGP1447 Full name: Vacuolar protein-sorting-associated protein 25 NCBI Accession No.: NP_115729 Synonyms: DERP9, EAP20, FAP20 Description: VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M Nacl Molecular Weight: 23.3 kDa(200aa), confirmed by MALDI-TOF Purity: > 85% by SDS - PAGE Concentration: 0.5 mg/ml (determined by Bradford assay) 15% SDS-PAGE (3ug) Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF General references: Bailey,S.D. et al. (2010) Diabetes Care 33 (10), 2250-2253 Talmud,P.J. et al. (2009) Am. J. Hum. Genet. 85 (5), 628-642 Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
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Genome-Wide Rnai Screen Reveals a Role for the ESCRT Complex in Rotavirus Cell Entry
Genome-wide RNAi screen reveals a role for the ESCRT complex in rotavirus cell entry Daniela Silva-Ayalaa, Tomás Lópeza, Michelle Gutiérreza, Norbert Perrimonb, Susana Lópeza, and Carlos F. Ariasa,1 aDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico; and bDepartment of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115 Edited by Mary K. Estes, Baylor College of Medicine, Houston, TX, and approved May 3, 2013 (received for review March 14, 2013) Rotavirus (RV) is the major cause of childhood gastroenteritis described that the VP8 protein of human RV strain HAL1166 worldwide. This study presents a functional genome-scale analysis and the human RV strains belonging to the most frequent VP4 of cellular proteins and pathways relevant for RV infection using genotypes (P4 and P8) bind to A-type histo-blood group antigens RNAi. Among the 522 proteins selected in the screen for their (5, 6). Integrin 2β1 has also been reported to serve as an attach- ability to affect viral infectivity, an enriched group that partic- ment receptor for some RV strains (7), although this integrin, as ipates in endocytic processes was identiﬁed. Within these pro- well as integrins vβ3 and xβ2 and the heat-shock protein 70 teins, subunits of the vacuolar ATPase, small GTPases, actinin 4, (HSC70), have been implicated mostly in a postattachment in- and, of special interest, components of the endosomal sorting teraction of the virus that might be involved in cell internalization complex required for transport (ESCRT) machinery were found.
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