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Eco Freinfly Synthesis and Characterization of Silver Nanoparticles Using Aquous Leaf Extract of Alphonsea Sclerocarpa and Apply It As Antimicrobial

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Eco Freinfly Synthesis and Characterization of Silver Nanoparticles Using Aquous Leaf Extract of Alphonsea Sclerocarpa and Apply It As Antimicrobial International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 Eco Freinfly Synthesis And Characterization Of Silver Nanoparticles Using Aquous Leaf Extract Of Alphonsea Sclerocarpa And Apply It As Antimicrobial Hassanain Hataf Jaber Altallal M.Sc. Nanobiotechnology, Department of Biotechnology Acharya Nagarjuna University, Guntur Abstract: To develop a reliable, eco- Green synthesized silver friendly and easy process for the nanoparticles are confirmed by synthesis of silver nanoparticles color change which was monitored using leave extracts of medicinal quantitatively by UV-Vis plant “Alphonsea sclerocarpa”. spectroscopy at 440nm (Fig.4). There has been an exponentially Further characterization with SEM, increasing interest in biological TEM and AFM analysis shows the synthesis of AgNPs. In this study, spherical, polydisperse AgNPs of AgNPs were synthesized by an particle size ranging from 5 to eco-friendly and convenient method 50nm with an average size of using Alphonsea sclerocarpa leaf 28.5nm (Fig.7,8,9). FTIR showed extract at ambient temperature. the structure, the respective bands Alphonsea sclerocarpa leaf extract of the synthesized nanoparticles, has been used as a reducing and the stretch of bonds. The agent for the synthesis of silver cytotoxicity analysis of the green nitrate into silver nanoparticles. synthesized silver nanoparticles International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 was observed that showed highest [2-6] changes in primary ways for antimicrobial activity against gram both individual molecules/atoms positive bacteria Staphylococcus and their relating bulk. Novel aureus (MTCC-6908) (Fig.11) purposes of nanoparticles and showed average zone of inhibition nanomaterials are becoming quickly of (26 mm), the Gram negative on different fronts because of their bacteria Pseudomonas citronellolis totally new or improved properties (MTCC-1191) (Fig.12) showed based on scale, their distribution average zone of inhibition of (18.3 and morphology [7,8]. mm) and the Fungi Candida albicans (MTCC-4748) (Fig.13) Nanobiotechnology is a showed average zone of inhibition branch of science that dealing of (22.2 mm) (Fig.10). However, between nanoscience and further investigations were needed biotechnology involving the to identify the scaling-up usage of application of biological systems for this extract on metallic nanoparticle the production, manipulation and synthesis and its applications as design of new functional nano- antimicrobial. scale materials [9]. It combines biological methods with physical and chemical procedures to INTRODUCTION: generate nano-scale particles with Nanotechnology is a vital unique functions. The synthesis of field of cutting edge research nanoparticles using green managing synthesis, procedure and technology is advantageous over manipulation of molecule's, sub chemical agents owing to their molecule's and atoms structure environmental anxieties. There is extending from roughly 1-100 nm significant to produce inorganic in size [1]. Inside this size range nanoparticles as they supply every one of the properties greater material properties with (chemical, physical and biological) effective resourcefulness [10]. International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 Nanobiotechnology is optics, chemical industries, presently one of the most dynamic electronics, space industries, disciplines of research in energy science, catalysis, light contemporary material science emitters, single electron transistors, whereby plants and different plant nonlinear optical devices, photo- products are finding an imperative electrochemical applications and use in the synthesis of many more to count on [12]. nanoparticles (NPs) [5,6]. MATERIALS AND Nanomedicine is the medical application of nanotechnology [11]. METHODS: Nanomedicine ranges from the medical applications of Alphonsea sclerocarpa plant: nanomaterials and biological devices, to nanoelectronic Scientific classification: biosensors, and even possible Kingdom: Plantae future applications of molecular nanotechnology such as biological (Unranked): Angiosperms machines [11]. (Unranked): Magnoliids Nanotechnology is swiftly Order: Magnoliales gaining renovation in a large number of fields such as health Family: Annonaceae care, cosmetics, biomedical, food Genus: Alphonsea and feed, drug-gene delivery, Species: A. Sclerocarpa [13] environment, health, mechanics, International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 Fig. 1 . Alphonsea sclerocarpa plant. 5-10 pairs, slender, pinnate, General description: prominent; intercostae reticulate as Alphonsea sclerocarpa is showen in (Fig. 1) [14-18]. small trees in size 10-15 m, braches rugose, glabrous; Culture Media: branchlets drooping. Leaves simple, Growth Media NO. 3 (Nutrient alternate, distichous, estipulate; Agar Medium): petiole 6-8 mm long, slender, To prepare Growth Media glabrous; lamina 3.5-10 x 1.5-4 NO. 3 (Nutrient Agar Medium) by cm, oblong, oblong-lanceolate, dissolving 1.0 g of Beet extract elliptic ovate; base cuneate or and 2.0 g of Yeast extract and 5.0 attenuate; apex obtuse, entire, g of Peptone and 5.0 g sodium coriaceous, glabrous; lateral nerves chloride NaCL powder and 15.0 g International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 of Agar in 1.0 L of distilled water dissolving 10.0g of Beet extract and mix it properly till homogeneity powder and 5.0 g of Yeast extract of the liquid and finally sterilizes it and 10.0g of Peptone and 20.0 g in Autoclave for 15 min in 15 lb of Glucose and 2.0g of Na2HPO4 and 121 Co [19]. and 5.0g of Sodium Acetate and Growth Media NO. 5 (YRPO 2.0g of Triammonium citrate and 0.2g MgSO4.7H2O and 0.2g of Medium): To prepare Growth Media MgSO4.4H2O and 15.0 g Agar and NO. 5 (YRPO Medium) by 1.0 ml of Tween 80 liquid in 1.0 L of distilled water and Adjust the dissolving 3.0 g of Yeast extract powder and 10.0g of Peptone and PH at 6.2-6.6 and mix it properly till homogeneity of the liquid and 20.0g Dextrose and 15.0 g of Agar in 1.0 L of distilled water and mix finally sterilizes it in Autoclave for 15 min in 15 lb and 121 Co [21]. it properly till homogeneity of the liquid and finally sterilizes it in Growth Media NO. 65 (Malt Autoclave for 15 min in 15 lb and Extract Agar Medium): 121 C[20]. To prepare Growth Media NO. 65 (Malt Extract Agar Medium) Growth Media NO. 11 (MRS by dissolving 3.0g of Malt extract Medium): powder and 3.0 g of Yeast extract and 5.0g of Peptone and 10.0 g of To prepare Growth Media Glucose and 20.0 g Agar in 1.0 L NO. 11 (MRS Medium) by of distilled water and Adjust the International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 PH at 6.2 and mix it properly till As mentioned bellow: homogeneity of the liquid and 3.3.1 Bacillus subtilis – (MTCC No. finally sterilizes it in Autoclave for 15 min in 15 lb and 121 Co [22]. 10407) 3.3.2 Staphylococcus aureus – Microorganisms Collection: (MTCC No. 6908) Provided us with bacterial 3.3.3 Pseudomonas citronellolis – and fungal cultures from Microbial (MTCC No. 1191) Type Culture Collection Institute of Microbial technology Sector 39- A, 3.3.4 Klebsiella pneumoniae – Chandigarh-160036, by Mineral oil (MTCC No. 9024) Preservation method at room temperature. Mineral oil 3.3.5 Lactobacillus acidophilus – Preservation method by prepare (MTCC No. 10307) tubes of heart infusion agar with a 3.3.6 Enterobacter aerogenes – short slant. For fastidious organisms, add fresh native or (MTCC No. 111) heated blood. Sterilize mineral oil 3.3.7 Candida albicans – (MTCC (liquid petrolatum) in hot air (170 Co for 1 hour). Grow a pure No. 4748) culture on the agar slant. When 3.3.8 Aspergillus niger – (MTCC good growth is seen, add sterile No. 9687) mineral oil to about 1 cm above the tip of the slant. Subculture when needed by scraping growth Preparation of leaf Extracts: from under the oil. Store at room Prepare Aqueous Extracts temperature. Transfer after 6–12 months. (Water): International Journal of Research p-ISSN: 2348-6848 e-ISSN: 2348-795X Available at https://edupediapublications.org/journals Volume 03 Issue 12 August 2016 The fresh and tender leaves distilled water in Broun bottle of Alphonsea sclerocarpa were (because it is sensitive to the light) collected from the South India. in room temperature. These were thoroughly washed Silver Nanoparticle (Ag NPs) with tap water and kept under shade at room temperature for Synthesis: about (20) days till they dried The aqueous solution of (1 completely. The dried leaves were mM) silver nitrate (AgNO3) was mechanically grinded and sieved to prepared to synthesize AgNPs. get fine powder. The (5 g) of (190 mL) of aqueous solution of (1 powdered leaves was mixed with mM) AgNO3 was slowly added to (100) ml of distilled water, the (10 mL) of Alphonsea sclerocarpa mixture were boiled in the stirrer aqueous leaf extract while stirring, heat for 10 min in order to for reduction into Ag ions and kept dismantle tearing the wall of plant at room temperature, the emulsion cells, and then nominate the turned to dark brown after 30 min. mixture through suppress and bowl This confirmed the synthesis of funnel using the nomination papers AgNPs in the mixture solution. It is (Whitman No.1). And this is well known that silver nanoparticles prepared crude aqueous extract exhibit dark brown color in and stored in a refrigerator at 4 °C aqueous solution due to excitation [23].
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