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JAALAS 2020 Board Examinations Past Or Present  Information Presented Is Believed to Be Reliable and Correct Though Not Guaranteed for Accuracy Francis J

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JAALAS 2020 Board Examinations Past Or Present  Information Presented Is Believed to Be Reliable and Correct Though Not Guaranteed for Accuracy Francis J DISCLAIMERS ACLAM has not reviewed or sanctioned this presentation No information presented is known to be included in the ACLAM JAALAS 2020 Board Examinations past or present Information presented is believed to be reliable and correct though not guaranteed for accuracy Francis J. Sun, DVM, DACLAM, MBA Not every article is included in this review Laboratory Animal Workshop 2021 I used the 2nd edition Blue Book 1 2 General study tips ACLAM certification site Know your Role Delineation Document Laboratory Animal Medicine, 3rd Edition Allocate your time accordingly The 3rd edition of Laboratory Animal Medicine is currently available in electronic format, with the hard cover version available soon. As it is an Trust yourself with your study habits ACLAM sanctioned textbook, it is considered fair game for the 2016 exam. When studying, please realize that there are over 3,000 questions Groups vs individual in our database. It will take the exam committee longer than a single Practice exams or note cards exam cycle to update every reference; however all questions for the 2016 exam that have the 2nd edition of the Blue Book as a reference Rest and health lifestyle are more beneficial for the last couple of will be fact checked against the 3rd edition of the Blue Book and days than cramming updated as necessary. Exam Material Cut Off Date The annual ACLAM Exam may contain questions on materials from any relevant publications up to Dec 31 of the year prior to the exam (Dec 31, 2019 for the 2020 exam). 3 4 ACLAM certification site ACLAM certification site ACLAM 2021 Certification Examination Reference List : This reference list provides the sources of the majority of the examination Domain 1: Management of Spontaneous and Experimentally questions for the 2021 exam. Journal articles used for the exam are typically those published within the last 5years. Induced Diseases and Conditions 25% This list is not meant to be all‐inclusive (e.g., it does not indicate the only Domain 2: Management of Pain and Distress 10% resources candidates should use to prepare for an upcoming examination). Note that the ACLAM Series Blue Books are heavily referenced and are Domain 3: Research 20% considered core references. Journals Domain 4: Animal Care 24% • Comparative Medicine, bimonthly publication of the American Domain 5: Regulatory Responsibilities 19% Association for Laboratory Animal Science, Memphis, TN. • Institute for Laboratory Animal Research (ILAR) Journal, a quarterly Domain 6: Education 2% publication of ILAR, the National Academies, Washington, DC. • Journal of the American Association for Laboratory Animal Science (JAALAS), the bimonthly publication of the American Association for Laboratory Animal Science (AALAS), Memphis, TN. 5 6 1 The Journal Article Abstract : Problem/issue, findings Introduction : informative about species, models, diseases, etc. Pictures! plus captions and conclusions JAALAS Volume 59, Discussion Article may not meet requirements to be testable material but could jog your memory about something you should go back and review that is clearly in the RDD No1 Poll Everywhere setup/test 7 8 Blood Pressure Reference Intervals for Ketamine-sedated Influence of Dietary Vitamin A and Iron Deficiency on Rhesus Macaques Hematologic Parameters and Body Weight of Young Male Wistar Rats 103 male and female rhesus of various body condition and ages Why studied? Micronutrient deficiency prominent public health concern Reference Interval – usually central 95% of values Vit A and iron 2 devices at 3 anatomic sites Child development Hypothesis : vit a and iron must be taken together to ensure adequate weight Device –no difference (abstract vs discussion) gain and hematologic parameters Location BP arm<leg<tail General suggestions of influences on growth and parameters Age, sex, body condition as variables confound findings 5 feed groups high and low iron, 3 levels of Vit A Recommend standardizing placement and measurement device Suggest even with adequate iron in diet, insufficient Vit A can cause serum iron levels to decrease Direct catheterization is ‘gold standard’ Iron deficient diet showed reduced Hgb What are indirect methods? All groups showed significant differences in reticulocyte count compared to control Doppler ultrasound Both Vit A and iron are essential to adequate growth and weight gain Manual sphygmomanometry Oscillometry 9 10 Effect of Repeated Exposure to Isoflurane on Nest Building and Burrowing in Mice Developed a refined scoring method for nest-building activity Nest building and burrowing have been recommended as noninvasive tools in the assessment of welfare in mice Hypothesis : burrowing and nest building would decrease after The scoring system was based on Answer B exposure to any of 3 stressors and scoring method would have agreement between observers A)The amount of time the mouse stayed in the square and x spots B)The height and width of the nests C)The temperature achievable and firmness D)How well hidden the mice would be Found their system to be objective. Nest building decreased after iso exposure but not to a second exposure. Burrowing did not change. Questioned applicability to welfare assessment 11 12 2 Tell-tale TINT : Does the Time to Incorporate into Nest Test Utility of Automated Feeding Data to Detect Social Instability Evaluate Postsurgical Pain or Welfare in Mice in a Captive Breeding Colony of Rhesus Macaques: a Case study of Intrafamily Aggression Use of ethologic framework of behavioral time-budgets is a Captive breeding colonies in large outdoor multimale, multifemale practical strategy for identifying pain in mice. social groups of several matrilineal families. When mice fail to perform a highly motivated behavior, this suggests Automated feeders in use with RFID micro chips implanted in NHPs that they are budgeting energy toward more biologically essential Do females or males determine the dominance hierarchy in rhesus activities (sleep, eat, drink) macaques? TINT : when mouse retrieves new nesting material and incorporates it Hypothesis : that inappetance among socially housed macaques may into an existing nest within 10 minutes be indicative of increased social stress and give evidence before the onset of overt aggression Hypothesis that negative TINT would correlate with other measures of pain Findings suggestive that stress-induced inappetance and/or increased night-feeding in key individuals may help detect emerging instability Confounded by analgesia from anesthetic (Ketamine/xylazine) Caloric decrease in deposed subfamily Confounded by lack of response to rescue analgesia (buprenorphine) Caloric decrease predicted severity of trauma 13 14 Comparing Mouse Health Monitoring Between Soiled-bedding Sentinel and Exhaust Air Dust Surveillance Programs Several pathogens are unreliably detected with soiled bedding sentinels or poorly transmitted by soiled bedding What does this image show in use? Ans D Which was not one of the organisms in the comparison? Answer A A)Hepa Filter A) EDIM B) Rodentibacter C) Mouse Norovirus D) Helicobacter B)Temperature and humidity sensor C)Air freshener Soiled bedding yielded equivalent detection as Exhaust Air dust with which organism that was compared? Answer C D)Collection media A) EDIM B) Rodentibacter C) Mouse Norovirus D) Helicobacter 15 16 Comparing Mouse Health Monitoring Between Soiled-bedding Evaluation of 3 Alcohol-based Agents for Presurgical Skin Sentinel and Exhaust Air Dust Surveillance Programs preparation in Mice Three prep agents MNV is a non enveloped RNA virus in which family? Answer C Iodine povacrylex and isopropyl alcohol – denatures proteins and damages bacterial DNA A) Adenoviridae B) Parvoviridae C) Caliciviridae D) Herpesviridae Chlorhexidine gluconate in isopropyl alcohol – denatures protein and Mainly shed in feces disrupts bacterial membranes Helicobacter – gram negative, microaerophilic organism found in Chlorhexidine gluconate solution – disrupt bacterial membranes gastroinstestinal flora and mainly transmitted fecal-oral No additional loss of core body temperature Rodentibacter – was called Pasteurella pneumotropica No evidence of dermatitis or skin irritation Transmitted poorly through dirty bedding All three prep agents were as effective as triple application of Respiratory-Sendai, F. rodentium povidine and alcohol in alternating fashion Unstable and enveloped virus 17 18 3 Effect of Cranial Flexion of Pelvic Limbs on Interlaminar Length of the Lumbosacral Space in Sternally and Laterally Recumbent Juvenile Duroc and Adult Yucatan Pigs The images to the right depict multiplanar sagittal reconstruction of Null Hypotheses the lumbar spine of a Yucatan pig in lateral recumbency Cranial hyperflexion of pelvic limbs would not increase LSS and LSA of What is the orientation of the pelvic limbs in the bottom image? the pigs in sternal or right lateral recumbency Answer A Sternal positioning would not increase LSS and LSA compared to lateral A) Cranial hyper flexion Findings B) Caudal hyper flexion Hyperflexion of pelvic limbs increases LSS and LSA in sternally recumbent adult Yucatan, laterally recumbent adult Yucatan, and juvenile Duroc C) Neutral with leg in extension Hyperflexing the pelvic limbs of juveniles in sternal recumbency did not D) Neutral with leg flexed at knee increase LSS 19 20 Institutional Animal Care and Use Committee postapproval Monitoring Programs : A Proposed Comprehensive Classification
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