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Y Chromosome Biology 321 The inheritance patterns discovered by Mendel are true for genes that are located on autosomes What is an autosome? 1 The fly room at Columbia University ~ 1920 l to r: Calvin Bridges, A. sturtevant, Thomas Hunt Morgan th Early 20 century fly guys What do the inheritance patterns of sex-linked traits look like? First look at experiments done in the early 1900’s by a fruifly geneticist named Thomas Hunt Morgan 2 The fruit fly Drosophila melanogaster has been used extensively in genetic research because it is a good experimental organism: • small size (2mm) 12 day generation time large broods of progeny • external anatomy provides for all sorts of possibilities for interesting phenotypic variation The complete DNA sequence of the fly genome was completed in 2000 3 Morgan was doing a routine transfer of his wild- type stocks when he noted a white-eyed male fly in among a stock of wild-type red-eyed animals What do we mean by wild-type phenotype? 4 Wild-type phenotype: the phenotype observed in the standard lab stock or seen most commonly in the wild population In Drosophila, red eye color is the wild-type phenotype 5 Morgan retrieved this white-eyed fly and did a series of crosses: Male fruitflies have a stereotyped courtship display involving following and wing-extension and vibration: see link below form or info http://fire.biol.wwu.edu/trent/trent/fruitflymating.jpg 6 It’s not just about white vs red eyes or curled vs straight wings There are many mutant strains of Drosophila where the male courtship display is abnormal. The fruitless mutation (next page) causes males to court other males as well as females. 7 . Genetics. 1989 April; 121(4): 773–785. See also: http://fire.biol.wwu.edu/trent/trent/NYTgayfruitflies.pdf 8 Jargon check Statement: Fruitless homozygotes court both male and female fruitflies What we really mean: Male fruitflies that are homozygous for a loss-of-function mutation in the fruitless gene court both male and female fruitflies. Wild-type males court females only. 9 Using models to explore the genetic control of behavior A male Drosophila fruitfly performs a ‘wing threat’, typical aggression behaviour, towards a rival male. Liming Wang and David Anderson show that the volatile pheromone cVA promotes male-to-male aggression by activating olfactory sensory neurons expressing the receptor protein, Or67d. This work opens the study of aggressive behaviour to detailed genetic manipulation and investigation. Cover image: Liming Wang & Michael Maire, Caltech. 10 One way a male Drosophila shows aggression is by "lunging," in which it rears up on its hind legs and snaps down with its forelegs on its opponent. (Credit: Caltech/Liming Wang and Michael Maire) Want to know more about genes and behavior? Check out this link: http://www.nature.com/scitable/topicpage/Behavioral-Genomics-29093 11 MEANWHILE back to MORGAN’S experiments [we will work through the crosses on the board] These results differed from typical Mendelian results in two ways: 1. The results of reciprocal crosses were different 2. F2 progeny ratios not in quarters Remember that when Mendel performed reciprocal crosses between his various plant lines, he always go the same result: when he crossed yellow with green he always got yellow F1 regardless of whether the pollen came from the green-seeded plant or the yellow-seeded plant This will almost always be true if the gene for the trait is located on an autosome Morgan interpreted the results of these crosses using information that he had about the chromosome constitution of Drosophila Morgan knew that Drosophila females had 4 regular chromosome pairs but that Drosophila males had 3 regular chromosome pairs plus a heteromorphic pair What does heteromorphic mean? 12 prophase of meiosis I in the testis of a salamander Heteromorphic means literally different form: a heteromorphic chromosome pair is a chromosome pair in which there is some difference in size or shape between the two chromosomes that pair 13 A Drosophila male has an X and a Y chromosome These X and Y chromosomes synapse and segregate during meiosis I like autosomal homologues would. To explain his data, Morgan proposed that a gene for eye color in Drosophila was present on the X chromosome with no counterpart on the Y chromosome Thus females would have two copies of the gene and males would have one copy 14 Assigning allele symbols Mendel’s style of allele notation would use the letter R (dominant phenotype is red eyes) as the gene designation with R= red (dominant) allele r= white recessive allele Drosophila geneticists assign a name and letter symbol to the gene based on the mutant phenotype. • So the gene that differs in the white and red-eyed flies is designated the white (w) gene • In Drosophila, wild-type allele is often indicated as a “+ “superscripted. • w+ = wildtype (red) allele • w = mutant (white) allele Fill in the genotypes of the reciprocal crosses: use Xw+ for red, wild-type allele and Xw for white allele. The results of the reciprocal crosses are consistent with the eye color gene being on the X chromosome with no counterpart on the Y chromosome 15 The naming of genes: Drosophila style http://tinman.vetmed.helsinki.fi/eng/drosophila.html 16 pop culture quiz : question 1 Like many Drosophila genes, the tinman gene is named for its mutant phenotype. What structure is missing in a fly with a mutated tinman gene? 17 lots of genes are named for their “loss-of-function” phenotypes [OK, we’re all adults here] question 2: what structure(s) are missing in flies mutated in the ken and barbie gene? 18 Don’t believe there is a gene with this name? Check it out at the InteractiveFly: http://www.sdbonline.org/fly/genebrief/ken&barbie.htm 19 Gene names: clever, obscure and often downright bad 20 Conventions that you must adhere to with respect to designating allele symbols: • if you are using upper and lower case letters, the upper case always symbolizes the dominant allele • a (+) superscript, always symbolizes the wild-type allele -- assuming that you have a reference point that indicates what phenotype is wildtype and what is mutant a wild-type allele is often, BUT NOT always dominant make no a priori assumptions regarding the dominance of a wild-type allele 21 Nettie Stevens was a talented cytogeneticist who discovered heteromorphic chromosome pair in insects. She was the first to propose that the X and Y -bearing sperm determined the sex of the zygote. 22 The Drosophila heteromorphic pair consists of the X and the Y chromosome: They synapse and segregate during meiosis like autosomal homologs Implicit in our analysis of Morgan’s crosses is the idea that sex chromosomes segregate into different gametes as paired homologs would But Morgan suggested that these chromosomes do not carry the same genes -- so why or how do they pair in meiosis? 23 Sex chromosomes can be divided into two regions Pairing: region of genetic homology where pairing occurs during meiosis Differential region: non-homologous region genes in this region have no counterpoint on the other sex chromosome 24 Hemizygous: genes located in the differential region of the X chromosome are hemizygous in males because males only have one copy of the gene Human X (left) and Y chromosomes Nature 423: 810 June 19, 2003 Tales of the Y chromosome 25 Gene and DiseaseMapView of autosomes, X & Y chromosomes http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=gnd&part=A272 26 View of Homo sapiens genome http://www.ncbi.nlm.nih.gov/projects/mapview/map_search.cgi?taxid=9606 Gene and DiseaseMapView of autosomes, X & Y chromosomes http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=gnd&part=A272 27 Chromosomal Sex-determining Mechanisms Shaded cells = diploid animals Organism Female Male Comments • Mammals XX XY Males produce two • Some different types of sperm: amphibians and 50% carry an X reptiles chromosome and 50% a • Many insects Y chromosome such as the fruitfly Drosophila • Some plants with male and female sexes 28 Organism female male Comments • Some insects XX XO Y chromosome is (including absent. Males have a spiders) single X chromosome • Some and produce two roundworms different types of (such as sperm: 50% bearing Caenorhabditis an X chromosome and elegans) 50% with no sex chromosome Pattern of sex linkage same as XX, XY species 29 Organism female male Comments • Birds ZW ZZ By convention, Z and W • Some insects are used to indicate the (such as moths sex chromosomes in and butterflies) these species. The Z • Some chromosome is amphibians and equivalent to the X reptiles such as chromosome. Females KOMODO produce two different dragons types of eggs: 50% carry the Z chromosome and 50% carry the W chromosome. 30 Organism female male Comments Bee, wasps and diploid haploid Males usually develop ants from unfertilized eggs; females from fertilized eggs. There are no sex chromosomes per se 31 What about non-chromosomal sex-determining mechanisms? 32 Genes in the NRY, or nonrecombining region of the Y (blue in diagram), have helped reveal the evolutionary history of the X and the Y. The region is so named because it cannot recombine, or exchange DNA, with the X. Only genes that still work are listed. About half have counterparts on the X (red); some of these are “housekeeping” genes, needed for the survival of most cells. Certain NRY genes act only in the testes (purple), where they likely participate in male fertility. 33 The X & Y chromosomes originated a few hundred million years ago from the same ancestral autosome. Y then is the Y a shadow
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