Cemip (NM 030728) Mouse Tagged ORF Clone – MR217955
Total Page:16
File Type:pdf, Size:1020Kb
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for MR217955 Cemip (NM_030728) Mouse Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: Cemip (NM_030728) Mouse Tagged ORF Clone Tag: Myc-DDK Symbol: Cemip Synonyms: 12H19.01.T7; 6330404C01Rik; 9930013L23Rik; AY007814; Kiaa1199 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >MR217955 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCTTCTGGGAGGCACGATGTCTCCCTCAAGATTGTGCTGGCCACGGGCTGCCTCCTCCTGGCCA ACTTCTCAGGGGCCTCATCTGCAGTGGCAACTGAGTGTCCTGATCAGAGTCCTGAGCTGCAGCCTTGGAG CCCTGGCCATAACAGAGACTATCAAGTACACATTGGCCATGGCAGGAAACTACTGCTCACCTCTTCTGCC ACAGTCCACTCCATCACTATCTCAGGGGGAGGAAAGCTTGTCATTAAAGACCACCATGAGCACATTGTGC TGCGTACCCGGTACATCCTGATTGATGACGGTGGAGAGCTGCATGCTGGGAGTGCCCTTTGCCCTTTTGA GGGCAATTTCAGTATTGTGCTGTATGGAAGGGCTGATGAAAACATCCTGCCAGACCCTTACTATGGCCTG AAGTACATCGGAGTAGACAAAGGAGGCACTCTTGAATTACATGGGCAGAAAAAGCTTTCCTGGACTTTTC TAAACAAGACCCTTCATCCTGGTGGCATGCAGGAGGGAGGATATTTTTTTGAAAGGAGCTGGGGCCACCG TGGAGTCATTGTCCATGTCATTGATGCCAAATTGGGCACAGTTGTCCATTCTGACCGGTTTGACACTTAC AGATCCAAAAAAGAGAGTGAACGTCTGGTCCAGTATTTGAATGCAGTGCCCGATGGCAGGATTCTTTCTG TCGCAGTGAATGATGAAGGTTCTAGAAACCTGGATGACACAGCCCGGAAGGCAATGACCAAACTGGGCAG CAAGCACTTCCTCCACCTTGGATTTAGACACCCTTGGAGCTTCATCACTGTGAAAGGAAATCCCTCCTCT TCAGTTGAAGACCACATTGAATACCATGGTCACAAAGGCTCGGCTGCTGCCCGGGTTTTCAAACTGTTCC AGACAGAGCATGGTGAACATTTCAATGTTTCTTCATCCAGCGAATGGGTTCAAGATGTGGAATGGACAGA GTGGTTTGACCATGATAAGGTGCCTCAGAGCAAAGGTGGGGAGAAAATTTCAGATCTGCGAGCAGCTTAC CCAGGAAAAATCTGCAATCGACCCATTGATATACAGGCCACAACAATGGATGGAGTCGCCCTCAGCACTG AGGTTGTCTACAAAAATGGCCAAGATTACAGGTTTGCTTGCTACACCCGGGGCAGAGCCTGCCGAAGCTA TCGAGTGCGATTCTTATGTGGAAAACCCGTGAGGCCCAAGCTCACGGTTAGCATCGACACCAACGTAAAC AGCACCATCCTGAGTCTGGTGGACAATGTGCGGTCATGGAGGCCTGGAGACACCCTGGTTGTTGCCAGCA CTGACTATTCGATGTACCAGGCAGAAGAGTTCCGGGTGCTCCCCTGCAAAGCTTGCACCTCTACACAGGT CAAGGTGGCAGGGAAACCACAGTACCTACATATTGGGGAGGAGATTGATGGTGTAGACATGCGGGCTGAG GTCGGACTCCTGACTCGGAACATTGTGGTGATGGGGGAGATGGAGGACAGATGCTACCCCTACACCAATC This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 4 Cemip (NM_030728) Mouse Tagged ORF Clone – MR217955 ACATCTGTGACTTCTTTGACTTCGATACCTTTGGGGGCCACATCAAGTTTGCACTGGGGTTTAAAGCAGC CCACTTGGAGGGCGTGGAGCTGAAGTACATGGGGCAGCAGCTGGTGGGCCAGTACCCAATTCACTTCCAC CTGGCTGGAGATTTGGATGAACAGGGAGGCTATGACCCCCCTACCTACATCAGGGACCTCTCCATTCATC ACACATTCTCCCGCTGCGTCACTGTCCATGGCTCCAACGGTCTGTTGATCAAGGATGTTGTGGGCTATAA TTCTTTGGGCCACTGCTTCTTCACTGAAGATGGGCCAGAGGAACGCAACACTTTTGACCACTGCCTTGGC CTCCTTGTTAAGTCAGGGACCCTTCTCCCATCCGATCGGGATAGCAGGATGTGCAAAGTGATCACAGAGG ACTCATACCCAGGGTACATCCCCAAGCCCAGGCAGGACTGCAATGCCGTGTCCACATTCTGGATGGCGAA TCCCAACAACAATCTCATCAACTGCGCAGCTGCAGGGTCCGAGGAAACTGGATTTTGGTTCATTTTTCAC CATGTGCCAACGGGCCCCTCTGTGGGAATGTACTCTCCAGGTTATTCTGAGCACATTCCACTGGGTAAAT TCTACAACAATCGAGCACATTCTAACTACCGGGCTGGCATGATCATAGACAATGGAGTCAAGACAACTGA GGCCTCAGCCAAGGATAAACGGCCCTTCCTCTCCATCATCTCTGCCAGGTACAGCCCTCACCAGGACGCA GACCCACTGAAACCTCGGGAACCAGCCATTATCCGACACTTTACTGCCTACAAGAACCAGGATCATGGAG CCTGGCTGCGGGGTGGGGATGTGTGGCTGGACAGCTGTAGGTTTGCTGATAATGGCATTGGTCTGACCCT GGCCAGTGGTGGAACCTTCCCATATGATGATGGCTCCAAGCAGGAGATAAAGAACAGCTTGTTTGTTGGC GAGAGTGGCAATGTGGGCACAGAAATGATGGACAACAGGATCTGGGGTCCAGGTGGCTTGGACCACAGTG GAAGGACCCTCCCTATTGGCCAGAATTTTCCCATTAGAGGAATTCAGTTCTACGATGGCCCCATCAACAT CCAGAACTGCACCTTCCGCAAGTTCGCGGCCCTCGAGGGCAGGCACACCAGTGCCTTGGCCTTCCGCCTA AACAATGCCTGGCAAAGCTGCCCTCACAACAATGTGACCAACATAGCCTTTGAGGATGTCCCGATTACTT CCAGAGTGTTCTTCGGAGAGCCCGGGCCCTGGTTCAACCAGCTGGACATGGACGGGGACAAGACATCTGT GTTCCATGATCTGGACGGCTCTGTGTCGGAGTACCCTGGCTCCTATCTCACTAAGGACGACAACTGGCTG GTCCGACACCCAGACTGCATCAATGTCCCAGACTGGAGAGGGGCCATCTGCAGTGGGCGCTATGCACAGA TGTACATCCAGGCCTACAAGAGCAGCAATCTGCGCATGAAGATCATTAAGAATGACTTCCCCAGCCACCC GCTCTACCTGGAGGGGGCCCTCACCAGGAGCACCCACTACCAGCAGTATCAGCCTGTCATCACCCTGCAG AAGGGCTATACTATCCACTGGGACCAGACAGCCCCCGCCGAGCTTGCCATCTGGCTCATCAACTTCAACA AGGGTGACTGGATCCGAGTGGGGCTCTGCTACCCAAGAGGCACCACCTTTTCCATCCTTTCAGATGTTCA CAATCGCCTGCTAAAGCAAACGTCCAAGACTGGGACCTTTGTGAGGACTCTGCAGATGGACAAAGTTGAG CAGAGCTATCCTGGCAGGAGCCACTACTACTGGGATGAGGATTCTGGGTTGCTCTTCTTGAAATTAAAAG CCCAGAACGAAAGGGAGAAATTTGCTTTCTGCTCCATGAAGGGCTGTGAGAGAATCAAGATTAAAGCTCT GCTCCCAAGAAATGCAGGCATCAGCGACTGCACAGCCACGGCTTACCCCAGGTTCACTGAGAGGGCCATA GTGGATGTACCAATGCCCAGGAAGCTCTTTGGGGCTCAGCTGAAGACAAAAGACCACTTCCTGGAAGTGA AGATGGAAAGTTCCAGGCAGCACTTCTTCCACCTTCGGAATGACTTCGCTTATATTGAAGTGGATGGGAG GAGGTACCCTTGCTCAGAAGATGGCATCCAGATAGTGGTGATTGATGGGAGTCGAGGTCACGTGGTGAGC CACGGCAGCTTCAGAAATGCCATTCTGCAGGGCATACCCTGGCAGCTCTTCAACTACGTGGCAGCTATCC CTGACAATTCCATAGTGCTCATGGCATCAAAGGGCAGATACATCACCAGAGGCCCATGGACCAGGGTGCT GGAAAAGCTTGGGGCAGACAAAGGTCTCAAGTTGAAAGAAAAGATGGCGTTCGTCGGCTTCAAAGGCAGC TTCCGACCCATCTGGGTGACTCTGGAGACTGAGGACCACAAAGCCAAAATCTTCCAAGTGGTGCCCATCC CTGTGGTGAGAAAGAAGAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 Cemip (NM_030728) Mouse Tagged ORF Clone – MR217955 Protein Sequence: >MR217955 protein sequence Red=Cloning site Green=Tags(s) MRASGRHDVSLKIVLATGCLLLANFSGASSAVATECPDQSPELQPWSPGHNRDYQVHIGHGRKLLLTSSA TVHSITISGGGKLVIKDHHEHIVLRTRYILIDDGGELHAGSALCPFEGNFSIVLYGRADENILPDPYYGL KYIGVDKGGTLELHGQKKLSWTFLNKTLHPGGMQEGGYFFERSWGHRGVIVHVIDAKLGTVVHSDRFDTY RSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDTARKAMTKLGSKHFLHLGFRHPWSFITVKGNPSS SVEDHIEYHGHKGSAAARVFKLFQTEHGEHFNVSSSSEWVQDVEWTEWFDHDKVPQSKGGEKISDLRAAY PGKICNRPIDIQATTMDGVALSTEVVYKNGQDYRFACYTRGRACRSYRVRFLCGKPVRPKLTVSIDTNVN STILSLVDNVRSWRPGDTLVVASTDYSMYQAEEFRVLPCKACTSTQVKVAGKPQYLHIGEEIDGVDMRAE VGLLTRNIVVMGEMEDRCYPYTNHICDFFDFDTFGGHIKFALGFKAAHLEGVELKYMGQQLVGQYPIHFH LAGDLDEQGGYDPPTYIRDLSIHHTFSRCVTVHGSNGLLIKDVVGYNSLGHCFFTEDGPEERNTFDHCLG LLVKSGTLLPSDRDSRMCKVITEDSYPGYIPKPRQDCNAVSTFWMANPNNNLINCAAAGSEETGFWFIFH HVPTGPSVGMYSPGYSEHIPLGKFYNNRAHSNYRAGMIIDNGVKTTEASAKDKRPFLSIISARYSPHQDA DPLKPREPAIIRHFTAYKNQDHGAWLRGGDVWLDSCRFADNGIGLTLASGGTFPYDDGSKQEIKNSLFVG ESGNVGTEMMDNRIWGPGGLDHSGRTLPIGQNFPIRGIQFYDGPINIQNCTFRKFAALEGRHTSALAFRL NNAWQSCPHNNVTNIAFEDVPITSRVFFGEPGPWFNQLDMDGDKTSVFHDLDGSVSEYPGSYLTKDDNWL VRHPDCINVPDWRGAICSGRYAQMYIQAYKSSNLRMKIIKNDFPSHPLYLEGALTRSTHYQQYQPVITLQ KGYTIHWDQTAPAELAIWLINFNKGDWIRVGLCYPRGTTFSILSDVHNRLLKQTSKTGTFVRTLQMDKVE QSYPGRSHYYWDEDSGLLFLKLKAQNEREKFAFCSMKGCERIKIKALLPRNAGISDCTATAYPRFTERAI VDVPMPRKLFGAQLKTKDHFLEVKMESSRQHFFHLRNDFAYIEVDGRRYPCSEDGIQIVVIDGSRGHVVS HGSFRNAILQGIPWQLFNYVAAIPDNSIVLMASKGRYITRGPWTRVLEKLGADKGLKLKEKMAFVGFKGS FRPIWVTLETEDHKAKIFQVVPIPVVRKKKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Restriction Sites: SgfI-MluI Cloning Scheme: This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 Cemip (NM_030728) Mouse Tagged ORF Clone – MR217955 Plasmid Map: ACCN: NM_030728 ORF Size: 4086 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_030728.3 RefSeq Size: 7114 bp RefSeq ORF: 4086 bp Locus ID: 80982 UniProt ID: Q5M9P4, Q8BI06 MW: 153 kDa Gene Summary: Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin- coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing. [UniProtKB/Swiss-Prot Function] This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 4.