Polyclonal Antibody to STRAD Beta / ILPIP 406-418) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP08962PU-N Polyclonal Antibody to STRAD beta / ILPIP 406-418) - Aff - Purified

Alternate names: ALS2CR2, CALS-21, PRO1038, Pseudokinase ALS2CR2, STE20-related kinase adapter protein beta, STRADB Quantity: 50 µg Concentration: 0.5 mg/ml Background: Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, is connected to transferase/kinase activity and ATP binding, it has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family. Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1 converting enzyme of Fas-induced apotosis. This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival, suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed. Uniprot ID: Q9C0K7 NCBI: NP_001193793.1 GeneID: 55437 Host: Goat Immunogen: Synthetic peptide from C-terminus of human ALS2CR2 AA Sequence: CDFPDEKDSYWEF Remarks: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Bovine, Dog (92%); Panda, Rabbit, Pig (85%) Format: State: Liquid purified Ig Purification: Immunoaffinity Chromatography Buffer System: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide Applications: ELISA: 1/32000. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml. Western Blot: 1 - 3 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody reacts to STE20-Related Kinase Adaptor Beta (STRADB/ALS2CR2) at aa 406-418. It is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP- beta (AAF71042.1). Species Reactivity: Tested: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Dog, Panda, Rabbit, Pig

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AP08962PU-N: Polyclonal Antibody to STRAD beta / ILPIP 406-418) - Aff - Purified

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: Heart: Formalin-Fixed, Paraffin- Embedded (FFPE)

HEK293 overexpressing ILPIP and probed with the antibody (mock transfection in first lane).

Antibody staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2