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Boophone Disticha
Micropropagation and pharmacological evaluation of Boophone disticha Lee Cheesman Submitted in fulfilment of the academic requirements for the degree of Doctor of Philosophy Research Centre for Plant Growth and Development School of Life Sciences University of KwaZulu-Natal, Pietermaritzburg April 2013 COLLEGE OF AGRICULTURE, ENGINEERING AND SCIENCES DECLARATION 1 – PLAGIARISM I, LEE CHEESMAN Student Number: 203502173 declare that: 1. The research contained in this thesis, except where otherwise indicated, is my original research. 2. This thesis has not been submitted for any degree or examination at any other University. 3. This thesis does not contain other persons’ data, pictures, graphs or other information, unless specifically acknowledged as being sourced from other persons. 4. This thesis does not contain other persons’ writing, unless specifically acknowledged as being sourced from other researchers. Where other written sources have been quoted, then: a. Their words have been re-written but the general information attributed to them has been referenced. b. Where their exact words have been used, then their writing has been placed in italics and inside quotation marks, and referenced. 5. This thesis does not contain text, graphics or tables copied and pasted from the internet, unless specifically acknowledged, and the source being detailed in the thesis and in the reference section. Signed at………………………………....on the.....….. day of ……......……….2013 ______________________________ SIGNATURE i STUDENT DECLARATION Micropropagation and pharmacological evaluation of Boophone disticha I, LEE CHEESMAN Student Number: 203502173 declare that: 1. The research reported in this dissertation, except where otherwise indicated is the result of my own endeavours in the Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg. -
Isolation and Identification of Bacterial Endophytes from Crinum Macowanii Baker
Vol. 17(33), pp. 1040-1047, 15 August, 2018 DOI: 10.5897/AJB2017.16350 Article Number: 6C0017758202 ISSN: 1684-5315 Copyright ©2018 Author(s) retain the copyright of this article African Journal of Biotechnology http://www.academicjournals.org/AJB Full Length Research Paper Isolation and identification of bacterial endophytes from Crinum macowanii Baker Rebotiloe F. Morare1*, Eunice Ubomba-Jaswa1,2 and Mahloro H. Serepa-Dlamini1 1Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, P. O. Box 17011 Doornfontein 2028, Johannesburg, South Africa. 2Water Research Commission, Lynnwood Bridge Office Park, Bloukrans Building, 4 Daventry Street, Lynnwood Manor, Pretoria, South Africa. Received 30 November, 2017; Accepted 4 July, 2018 The widespread distribution of Crinum macowanii across the African continent has entrenched the plant’s medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and Staphylococcus species C6 were isolated from fresh C. macowanii bulb and their phenotypic and genotypic profiles verified by Gram staining and 16S rRNA gene sequencing; respectively. The latter was used to construct a phylogenetic tree that showed similarities (higher than 50 bootstrap values) among the endophytic bacterial isolates. Chemical analysis of bacterial endophytes was done by extracting the crude extracts of each endophyte. Antibacterial activity of each endophyte was performed against a few selected bacterial pathogenic strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus cereus) using the disk diffusion method with Streptomycin used as a positive control. -
Sell Cut Flowers from Perennial Summer-Flowering Bulbs
SELL CUT FLOWERS FROM PERENNIAL SUMMER-FLOWERING BULBS Andy Hankins Extension Specialist-Alternative Agriculture, Virginia State University Reviewed by Chris Mullins, Virginia State University 2018 Commercial producers of field-grown flower cut flowers generally have a wide selection of crops to sell in April, May and June. Many species of annual and especially perennial cut flowers bloom during these three months. Many flower crops are sensitive to day length. Crops that bloom during long days such as larkspur, yarrow, peonies and gypsophila cannot be made to bloom after the summer equinox on June 21st. Other crops such as snapdragons may be day length neutral but they are adversely affected by the very warm days and nights of mid-summer. It is much more challenging for Virginia cut flower growers to have a diverse selection of flower crops for marketing from July to September when day length is getting shorter and day temperatures are getting hotter. Quite a few growers offer the same inventory of sunflowers, zinnias, celosia and gladiolas during the middle of the summer because everything else has come and gone. A group of plants that may offer new opportunities for sales of cut flowers during mid-summer are summer-flowering bulbs. Many of these summer-flowering bulbs are tropical plants that have only become available in the United States during the last few years. The first question that growers should ask about any tropical plant recommended for field planting is, " Will this species be winter hardy in Virginia?" Many of the bulb species described in this article are not very winter hardy. -
TELOPEA Publication Date: 13 October 1983 Til
Volume 2(4): 425–452 TELOPEA Publication Date: 13 October 1983 Til. Ro)'al BOTANIC GARDENS dx.doi.org/10.7751/telopea19834408 Journal of Plant Systematics 6 DOPII(liPi Tmst plantnet.rbgsyd.nsw.gov.au/Telopea • escholarship.usyd.edu.au/journals/index.php/TEL· ISSN 0312-9764 (Print) • ISSN 2200-4025 (Online) Telopea 2(4): 425-452, Fig. 1 (1983) 425 CURRENT ANATOMICAL RESEARCH IN LILIACEAE, AMARYLLIDACEAE AND IRIDACEAE* D.F. CUTLER AND MARY GREGORY (Accepted for publication 20.9.1982) ABSTRACT Cutler, D.F. and Gregory, Mary (Jodrell(Jodrel/ Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, England) 1983. Current anatomical research in Liliaceae, Amaryllidaceae and Iridaceae. Telopea 2(4): 425-452, Fig.1-An annotated bibliography is presented covering literature over the period 1968 to date. Recent research is described and areas of future work are discussed. INTRODUCTION In this article, the literature for the past twelve or so years is recorded on the anatomy of Liliaceae, AmarylIidaceae and Iridaceae and the smaller, related families, Alliaceae, Haemodoraceae, Hypoxidaceae, Ruscaceae, Smilacaceae and Trilliaceae. Subjects covered range from embryology, vegetative and floral anatomy to seed anatomy. A format is used in which references are arranged alphabetically, numbered and annotated, so that the reader can rapidly obtain an idea of the range and contents of papers on subjects of particular interest to him. The main research trends have been identified, classified, and check lists compiled for the major headings. Current systematic anatomy on the 'Anatomy of the Monocotyledons' series is reported. Comment is made on areas of research which might prove to be of future significance. -
Aβ) from South African Plants Using an Automated HPLC/SPE/HPLC Coupling System
Original Article Biomol Ther 19(1), 90-96 (2011) Rapid Identification of Bioactive Compounds Reducing the Production of Amyloid β-Peptide (Aβ) from South African Plants Using an Automated HPLC/SPE/HPLC Coupling System Hak Cheol Kwon1, Jin Wook Cha1, Jin-Soo Park1, Yoon Sun Chun2, Nivan Moodley3, Vinesh J. Maharaj3, Sung Hee Youn2, Sungkwon Chung2,* and Hyun Ok Yang1,* 1Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, 2Department of Physiology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea, 3Biosciences, CSIR, RSA, PO Box 395, Pretoria, 0001, South Africa Abstract Automated HPLC/SPE/HPLC coupling experiments using the Sepbox system allowed the rapid identifi cation of four bioactive principles reducing the production of amyloid β-peptide (Aβ) from two South African plants, Euclea crispa subsp. crispa and Cri- num macowanii. The structures of biologically active compounds isolated from the methanol extract of Euclea crispa subsp. crispa were assigned as 3-oxo-oleanolic acid (1) and natalenone (2) based on their NMR and MS data, while lycorine (3) and hamayne (4) were isolated from the dichloromethane-methanol (1:1) extract of Crinum macowanii. These compounds were shown to inhibit the production of Aβ from HeLa cells stably expressing Swedish mutant form of amyloid precursor protein (APPsw). Key Words: HPLC/SPE/HPLC, Alzheimer’s disease, Amyloid β-peptide, Euclea crispa subsp. crispa, Crinum macowanii INTRODUCTION of many novel therapeutic agents (John, 2009). However, the classical bioactivity-guided fractionation for the purifi cation As one of the most serious health problems worldwide, Al- and identifi cation of biologically active compounds from plants zheimer’s disease (AD) is the most common form of age-relat- extracts can be tedious process with long time frames (Clark- ed dementia (Lim et al., 2006). -
Networks in a Large-Scale Phylogenetic Analysis: Reconstructing Evolutionary History of Asparagales (Lilianae) Based on Four Plastid Genes
Networks in a Large-Scale Phylogenetic Analysis: Reconstructing Evolutionary History of Asparagales (Lilianae) Based on Four Plastid Genes Shichao Chen1., Dong-Kap Kim2., Mark W. Chase3, Joo-Hwan Kim4* 1 College of Life Science and Technology, Tongji University, Shanghai, China, 2 Division of Forest Resource Conservation, Korea National Arboretum, Pocheon, Gyeonggi- do, Korea, 3 Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, United Kingdom, 4 Department of Life Science, Gachon University, Seongnam, Gyeonggi-do, Korea Abstract Phylogenetic analysis aims to produce a bifurcating tree, which disregards conflicting signals and displays only those that are present in a large proportion of the data. However, any character (or tree) conflict in a dataset allows the exploration of support for various evolutionary hypotheses. Although data-display network approaches exist, biologists cannot easily and routinely use them to compute rooted phylogenetic networks on real datasets containing hundreds of taxa. Here, we constructed an original neighbour-net for a large dataset of Asparagales to highlight the aspects of the resulting network that will be important for interpreting phylogeny. The analyses were largely conducted with new data collected for the same loci as in previous studies, but from different species accessions and greater sampling in many cases than in published analyses. The network tree summarised the majority data pattern in the characters of plastid sequences before tree building, which largely confirmed the currently recognised phylogenetic relationships. Most conflicting signals are at the base of each group along the Asparagales backbone, which helps us to establish the expectancy and advance our understanding of some difficult taxa relationships and their phylogeny. -
Aloes and Lilies of Ethiopia and Eritrea
Aloes and Lilies of Ethiopia and Eritrea Sebsebe Demissew Inger Nordal Aloes and Lilies of Ethiopia and Eritrea Sebsebe Demissew Inger Nordal <PUBLISHER> <COLOPHON PAGE> Front cover: Aloe steudneri Back cover: Kniphofia foliosa Contents Preface 4 Acknowledgements 5 Introduction 7 Key to the families 40 Aloaceae 42 Asphodelaceae 110 Anthericaceae 127 Amaryllidaceae 162 Hyacinthaceae 183 Alliaceae 206 Colchicaceae 210 Iridaceae 223 Hypoxidaceae 260 Eriospermaceae 271 Dracaenaceae 274 Asparagaceae 289 Dioscoreaceae 305 Taccaceae 319 Smilacaceae 321 Velloziaceae 325 List of botanical terms 330 Literature 334 4 ALOES AND LILIES OF ETHIOPIA Preface The publication of a modern Flora of Ethiopia and Eritrea is now completed. One of the major achievements of the Flora is having a complete account of all the Mono cotyledons. These are found in Volumes 6 (1997 – all monocots except the grasses) and 7 (1995 – the grasses) of the Flora. One of the main aims of publishing the Flora of Ethiopia and Eritrea was to stimulate further research in the region. This challenge was taken by the authors (with important input also from Odd E. Stabbetorp) in 2003 when the first edition of ‘Flowers of Ethiopia and Eritrea: Aloes and other Lilies’ was published (a book now out of print). The project was supported through the NUFU (Norwegian Council for Higher Education’s Programme for Development Research and Education) funded Project of the University of Oslo, Department of Biology, and Addis Ababa University, National Herbarium in the Biology Department. What you have at hand is a second updated version of ‘Flowers of Ethiopia and Eritrea: Aloes and other Lilies’. -
The Naturalized Vascular Plants of Western Australia 1
12 Plant Protection Quarterly Vol.19(1) 2004 Distribution in IBRA Regions Western Australia is divided into 26 The naturalized vascular plants of Western Australia natural regions (Figure 1) that are used for 1: Checklist, environmental weeds and distribution in bioregional planning. Weeds are unevenly distributed in these regions, generally IBRA regions those with the greatest amount of land disturbance and population have the high- Greg Keighery and Vanda Longman, Department of Conservation and Land est number of weeds (Table 4). For exam- Management, WA Wildlife Research Centre, PO Box 51, Wanneroo, Western ple in the tropical Kimberley, VB, which Australia 6946, Australia. contains the Ord irrigation area, the major cropping area, has the greatest number of weeds. However, the ‘weediest regions’ are the Swan Coastal Plain (801) and the Abstract naturalized, but are no longer considered adjacent Jarrah Forest (705) which contain There are 1233 naturalized vascular plant naturalized and those taxa recorded as the capital Perth, several other large towns taxa recorded for Western Australia, com- garden escapes. and most of the intensive horticulture of posed of 12 Ferns, 15 Gymnosperms, 345 A second paper will rank the impor- the State. Monocotyledons and 861 Dicotyledons. tance of environmental weeds in each Most of the desert has low numbers of Of these, 677 taxa (55%) are environmen- IBRA region. weeds, ranging from five recorded for the tal weeds, recorded from natural bush- Gibson Desert to 135 for the Carnarvon land areas. Another 94 taxa are listed as Results (containing the horticultural centre of semi-naturalized garden escapes. Most Total naturalized flora Carnarvon). -
The Qualitative and Quantitative Phytochemical Investigation of Crinum Species in Ethiopia
International Journal of Photochemistry and Photobiology 2019; 3(1): 1-9 http://www.sciencepublishinggroup.com/j/ijpp doi: 10.11648/j.ijpp.20190301.11 ISSN: 2640-4281 (Print); ISSN: 2640-429X (Online) The Qualitative and Quantitative Phytochemical Investigation of Crinum Species in Ethiopia Asnakech Senbeta 1, *, Tesfaye Awas 2, Abdella Gure 3 1Crop & Horticulture Biodiversity Directorate, Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia 2Forest & Range Land Plants Biodiversity Directorate, Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia 3Department of Forestry, Wondo Genet College of Forestry & Natural Resources, Hawassa Universities, Wondo Genet, Ethiopia Email address: *Corresponding author To cite this article: Asnakech Senbeta, Tesfaye Awas, Abdella Gure. The Qualitative and Quantitative Phytochemical Investigation of Crinum Species in Ethiopia. International Journal of Photochemistry and Photobiology. Vol. 3, No. 1, 2019, pp. 1-9. doi: 10.11648/j.ijpp.20190301.11 Received : May 13, 2019; Accepted : June 13, 2019; Published : July 2, 2019 Abstract: Medicinal plants have a long history of use in most communities all over the world. Plants have the ability to synthesize a wide diversity of chemical compounds that uses to perform important biological functions. Many of Genus Crinum has been broadly used in traditional and ethno-medicines in the world wide. The aims of this study were to investigate the qualitative and quantitative phytochemicals constituents of the four species of the genus Crinum that exists in Ethiopia. All experiments were follow standard procedures. For the purpose of conducting phytochemical analyses on the four species each, three to six bulbs were collected from Field Gene Banks, Botanical Gardens and local fields. The bulb samples were cleaned, dried and crushed into powder. -
CBD Second National Report
FINAL VERSION Please provide the following details on the origin of this report. Contracting Party Lebanon National Focal Point Full name of the institution: Ministry of the Environment Name and title of contact officer: Ms. Lara Samaha CBD Focal Point Mailing address: 70-1091 Antelias Lebanon Telephone: +961-4-522-222 Ext. 455 Fax: +961-4-525-080 E-mail: [email protected] Contact officer for national report (if different) Full name of the institution: Ministry of Environment Name and title of contact officer: Dr. Berj Hatjian Director General Mailing address: 70-1091 Antelias Lebanon Telephone: +961-4-522-222 Ext. 500 Fax: +961-4-525-080 E-mail: [email protected] Submission Signature of officer responsible for submitting national report: Date of submission: EXECUTIVE SUMARY The Second National Report of Lebanon was developed to give a comprehensive perspective of Lebanon’s status in implementing the Convention on Biological Diversity. Taking into consideration the nature of the report template and the absence of a formal national body that monitors and guides biodiversity related activities in Lebanon, the authors decided to adopt a participatory approach for the completion of this report to include all stakeholders’ inputs and ensure a representative national perspective. The report includes documented and verbally relayed information, in addition, it reflects prevalent perspectives of 54 stakeholders who were interviewed from various sectors of the society and who currently constitute the bulk of individuals involved in biodiversity related activities in Lebanon. Lebanon is deficient in the following areas: taxonomy, monitoring, alien species, ex-situ, sustainable use, incentive measures, genetic resources, technology transfer, technical and scientific cooperation, biotechnology and information exchange. -
Activity of a Methanolic Extract of Zimbabwean Crinum Macowanii Against Exotic RNA Viruses in Vitro
PHYTOTHERAPY RESEARCH, VOL. 8, 121-122 (1994) SHORT COMM lJ NlCA TI0N Activity of a Methanolic Extract of Zimbabwean Crinum macowanii against Exotic RNA Viruses in vitro Zvitendo J. Duri Chemistry Department, University of Zimbabwe, PO Box MP 167, Mount Pleasant, Harare, Zimbabwe John P. Scovill* Toxicology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA John W. Huggins Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA The in uitro antiviral activity of a freeze-dried methanolic extract of the bulbs of Zimbabwean Crinurn mucowanii was determined in Vero cells infected with a number of exotic RNA viruses. At a concentration of 32 &mL, the crude methanolic extract reduced by 100% the viral cytopathic effect in Vero cells infected with yellow fever virus, and caused a 70% inhibition of the viral cytopathic effect in cells infected with Japanese encephalitis virus. No evidence of cytotoxicity was observed at this concentration. The freeze-dried extract inhibited the replication of the above mentioned viruses by 100% at a concentration of 10pg/mL. No cytotoxicity was seen at this concentration. Keywords: Crinum macowanii; yellow fever virus; Japanese encephalitis virus; Punta Toro virus; Venezuelan equine encephalitis virus. INTRODUCTION variety of amaryllis species. In this paper we report the initial observation of antiviral activity of a crude prep- aration of Crinum macowanii. Extracts of Crinum macowanii (vlei lily, dururu (Shona), umduze (Ndebele)) have a number of appli- cations in the traditional medicine of Zimbabwe. Infusions of the bulb of the plant are used to relieve MATERIALS AND METHODS backache, as an emetic, or to increase lactation in animal and human mothers. -
In Vitro Propagation of Haemanthus Pumilio and H. Albiflos (Amaryllidaceae) and the Population Genetics of H
In Vitro Propagation of Haemanthus pumilio and H. albiflos (Amaryllidaceae) and the population genetics of H. pumilio By Panashe Kundai Madzivire Thesis presented in partial fulfilment of the requirements for the degree of Master of Science in the Faculty of Science at Stellenbosch University Supervisor: Paul N Hills Co-supervisor: Léanne L. Dreyer March 2021 i Stellenbosch University https://scholar.sun.ac.za DECLARATION By submitting this thesis electronically, I declare that the entirety of the work contained therein is my own, original work, that I am the sole author thereof (save to the extent explicitly otherwise stated), that reproduction and publication thereof by Stellenbosch University will not infringe any third party rights and that I have not previously in its entirety or in part submitted it for obtaining any qualification Copyright © 2021 Stellenbosch University All rights reserved ii Stellenbosch University https://scholar.sun.ac.za ENGLISH - ABSTRACT Haemanthus albiflos and H. pumilio are members of the Amaryllidaceae. H. albiflos is a widespread evergreen plant, while H. pumilio is an endangered narrow endemic species with only two known populations remaining. These populations, remnants of the one recently transferred from Wellington to the Stellenbosch University Botanical gardens and another in the Duthie Reserve in Stellenbosch, present with vastly different morphologies. It is therefore vital to understand the phylogenetics and population genetics of H. pumilio as well as to design a method of in vitro propagation to increase the numbers of these plants. This study analysed the phylogenetics of H. pumilio using non-coding nuclear (Internal transcribed spacer), plastid (trnL-trnF intergenic spacer) and mitochondrial (nadi1477) gene regions and the population genetics using inter-simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphisms.