DOCSLIB.ORG

Micropropagation of Gloriosa Species and Their Potential As a Source of Colchicine

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Micropropagation of Gloriosa Species and Their Potential As a Source of Colchicine MICROPROPAGATION OF GLORIOSA SPECIES AND THEIR POTENTIAL AS A SOURCE OF COLCHICINE A thesis submitted for the degree of Doctor of Philosophy by J ayasiri Ranatunga School of Biochemistry and Molecular Genetics The University of New South Wales December, 1994 CERTIFICATE OF ORIGINALITY I hereby declare that this submission is my own work and that, to the best of my knowledge and belief, it contains no material previously published or written by another person nor material which to a substantial extent has been accepted for the award of any other degree or diploma of a university or other institute of higher learning, except where due acknowledgement is made in the text. (Signed) CONTENTS SUMMARY i ACKNOWLEDGEMENTS iii ABBREVIATIONS iv CHAPTER 1 - INTRODUCTION 1 1. 1 Genus Gloriosa 1 1. 2 Plant Tissue Culture 6 1. 2.1 Botanical Aspects of In Vitro Propagation 9 1.2.2 Methods of In Vitro Propagation 9 1.2.2.1 Callusing 9 1.2.2.2 Adventitious Bud Fonnation 10 1.2.2.3 Axillary Bud Fonnation 11 1. 2. 3 General In Vitro Procedure for Micropropagation 11 1.2.4 In Vitro Propagation of Monocotyledonous Plants 13 1.2.5 In Vitro Propagation of Gloriosa species 17 1. 3 Secondary Metabolites 19 1. 3. 1 Colchicine 26 1. 3. 1. 1 Occurrence in Plants 28 1. 3. 1. 2 Isolation, Purification and Quantitation of Colchicine From Plants 29 1.3.1.3 Biosynthesis of colchicine 31 1. 4 Project Aims 34 CHAPTER 2 - MATERIALS AND METHODS 35 2.1 Plant Materials 35 2.2 Chemicals 35 2.3 Sterile Technique 37 2.4 Aseptic Seed Gennination 37 2.5 Aseptic Tuber Sprouting 37 2.6 Callus Initiation 38 2.7 Proliferation and Maintenance of Callus 38 2.8 Root Cultures 39 2.9 Growth Rate of Callus 39 2.10 Initiation of Shoots from Tuber Explants 39 2.11 Initiation of Shoots from Shoot Bases 40 2.12 Initiation of Shoots from Root-Coleoptile Axis of Seedlings 41 2.13 Initiation of Shoots from Callus 42 2.14 Maintenance of Stock Cultures 42 2.15 Multiplication of Shoots 42 2.16 Replacement of NAA by IAA in Shoot Multiplication Medium 43 2.17 Alteration of Ammonium and Nitrate Concentration in Shoot Multiplication Medium 43 2.18 Alteration of Sucrose Concentration in Shoot Multiplication Medium 44 2.19 Development of Roots and Tubers 44 2.20 Effect of Sucrose on Development of Roots and Tubers 44 2.21 Effect of ABA and GA3 on Development of Roots and Tubers 45 2.22 Deflasking and Transferring to Soil 45 2.23 Micropropagation Growth Measurements 45 2.24 Statistical Analysis of Data 46 2.25 Extraction of Plant Tissues 46 2.26 Partial Purification of Colchicine 46 2.27 HPLC Analysis of Extracts 47 2.28 Mass Spectrometry 47 2.29 Thin Layer Chromatography 47 2.30 Counting of Radioactivity 48 2.31 Dry Weight Determination 48 2.32 Addition of Putative Colchicine Precursors in Liquid Shoot Multiplication Medium 48 2.33 Distribution of Colchicine in Mature Gloriosa simplex Tissues 48 2.34 Screening of High Yielding Cell Lines for Production of Colchicine and Conservation through Plant Regeneration 49 CHAPTER 3 - RESULTS - CALLUS 50 3. 1 Callus Cultures Derived From Mature Tuber Explants 50 3. 2 Callus Cultures Derived From Mature Seedling Ex plants 52 3. 3 Proliferation and Maintenance of Undifferentiated Callus 55 3.3.1 G. superba 55 3.3.2 G. simplex 57 3.4 Proliferation and Maintenance of Differentiated Callus 57 CHAPTER 4 - RESULTS - INITIATION OF SHOOTS 59 4.0 Introduction 59 4.1 Initiation of Shoots 60 4.1.1 Initiation of Shoots From Tuber Explants 60 4.1. 2 Initiation of Shoots From Shoot Base Explants 67 4.1.3 Initiation of Shoots From Root-Coleoptile Axes of Seedlings 71 4.2 Tuber Initiation From Tuber Explants 75 4.3 Tuber Initiation from Root-Coleoptile Axis Explants 80 CHAPTER 5 - RESULTS - SHOOT AND TUBER MULTIPLICATION 83 5. 0 Shoot and Tuber Multiplication 83 5. 1 G. rothschildiana 83 5. 1.1 Multiplication of Cultures Derived From Tuber Explants 83 5. 1. 2 The Effect of Altered Inorganic Nitrogen Concentration and Composition on Shoot and Tuber Multiplication 88 5. 1. 3 The Effect of Altered Sucrose Concentration on Shoot and Tuber Multiplication 91 5 .1.4 Multiplication of Cultures Derived From Shoot Base Explants 91 5 .1. 5 Multiplication of Cultures Derived From Root-Coleoptile Axes of Seedlings 92 5. 2 G. superba 94 5. 2.1 Multiplication of Cultures Derived From Tuber Explants 94 5.2.2 Multiplication of Cultures Derived From Shoot Base Ex plants 95 5.2.3 Multiplication of Culturres Derived From Root-Coleoptile Axes of Seedlings 96 5. 3. G. simplex 98 5.3.1 Multiplication of Cultures Derived From Tuber Explants 98 5.3.2 Multiplication of Cultures Derived From Shoot Base Explants 99 5.3.3 Multiplication of Cultures Derived From Root-Coleoptile Axis of Seedlings 100 5.3.4 Multiplication of Shoots Derived From Undifferentiated Callus 100 5 .4 G. verschuurii 102 5.4.1 Multiplication of Cultures Derived From Tuber Explants 102 5.5 G. richmondensis 103 5.5.1 Multiplication of Cultures Derived from Tuber Explants 103 CHAPTER 6 - RESULTS - ROOT AND TUBER DEVELOPMENT 104 6.0 Root and Tuber Development 104 6.1 Root and Tuber Development of Gloriosa species 104 6.1.1 The Effect of Sucrose Concentration on Root andTuber Development 106 6.1.2 The Effect of ABA and GA3 on Root and Tuber Development of G. rothschildiana 110 CHAPTER 7 - RESULTS - ESTABLISHMENT IN SOIL 111 7. 0 Establishment in Soil 111 7. 1 Survival Rates of In Vitro Generated Gloriosa Species in Soil 111 7. 2 Yield of Tubers After the First Growth Cycle under Glasshouse Conditions 112 7. 3 Yield of Tubers After Second and Third Growth Cycles under Glasshouse Conditions 113 7.4 The Effect of Potting Medium on Tuber Growth of G. superba. 115 7 .5 Micropropagation Protocol For Gloriosa Species 116 CHAPTER 8 - RESULTS - QUANTITATION OF COLCHICINE:DEVELOPMENT OF METHODS 118 8. 0 Extraction, Purification and Quantitation of Colchicine from Gloriosa 118 8. 1 Extraction and Purification from Plant Tissue 118 8. 2 Quantitation of Colchicine 123 8. 3 Purity of the Internal Standard ([3H]-Colchicine) 123 CHAPTER 9 - RESULTS - QUANTITATION OF COLCHICINE IN IN VITRO AND IN VIVO TISSUES 126 9.0 Colchicine Content of in viva and in vitro Tissues of Gloriosa species and in viva Tissues of Sandersonia aurantiaca 126 t 9 .1 Colchicine Con4ent of in viva Tissue 126 9 .1.1 Colchicine Content of Glasshouse Grown Gloriosa and Sandersonia aurantiaca 126 9 .1. 2 Distribution of Colchicine within a Mature G. simplex Vine and a Tuber 128 9.2 Colchicine Content of In Vitro Cultures 130 9.2.1 Colchicine Content of Shoot Initiation Cultures (Stage I) 130 9.2.2 Colchicine Content of Shoot Multiplication Cultures (Stage II) 131 d 9. 2. 3 Manipulation of Multiplication Medium for Increaset_Yield of Colchicine From G. rothschildiana Shoot Cultures 137 9.2.4 The Effect of Putative Colchicine Precursors on the Colchicine Content of Shoots and Tubers of G. superba 140 9. 2. 5 Colchicine Content of Root and Tuber Development Cultures (Stage III) 141 9. 3 Colchicine From In Vitro Generated Plants When Grown Under Glasshouse Conditions 145 9. 4 Colchicine from Undifferentiated and Redifferentiated Callus 14 7 9 .4.1 Colchicine Concentration of G. rothschildiana and G. simplex Differentiated Callus 147 9.4.2 Colchicine Concentrations of G. simplex Undifferentiated Cells 148 9. 5 A Protocol to Screen and Recover Cell Lines Highly Productive for Colchicine 149 CHAPTER 10 - DISCUSSION 151 Initiation and Maintenance of Callus Cultures 151 Micropropagation 154 Colchicine 168 Concluding Remarks 175 REFERENCES 177 APPENDICES 192 (i) SUMMARY Gloriosa species are increasing in popularity in the cut flower industry and they are also a potential source of the phannaceutical, colchicine. However their propagation on a commercial scale is hampered by the slow rate of vegetative multiplication. Furthermore, little information is available on the distribution of colchicine in the genus, except G. superba. This thesis describes studies on the in vitro micropropagation of G. rothschildiana, G. superba, G. simplex, G. verschuurii and the cultivar G. richmondensis. In addition colchicine was quantitated from in vivo and in vitro generated tissues of the above species, growing under identical conditions. A successful protocol for the rapid micropropagation of these species was developed which combined good shoot initiation, a high rate of multiplication and tuber initiation, followed by root and tuber development. Plantlets could then be directly transferred to soil without acclimatization. The effects of different explants, various auxins and cytokinins, and basal media on shoot and tuber initiation and subsequent multiplication were also determined. Juvenile tuber tissues made the best explants and Woody Plant medium was superior to that of Murashige and Skoog,. The best plant growth regulator (PGR) combination was 10 µM NAA and either 20µM or 40 µM BAP for shoot initiation and either 10 µM IAA and 40 µM BAP or 1 µM IAA and 20 µM BAP for multiplication. Root and tuber development was best in the absence of plant growth regulators. The in vitro cloned plants grew normally and produced flowers during the third growth cycle in soil. Increasing the sucrose concentration in root and tuber development medium from 2% to 4%, doubled the number of tubers per replicate for G. superba, while that for G. rothschildiana increased about 25%. Tuber weight per replicate doubled for both species. The optimum concentration of nitrogen was 30mM at a 1 :2 ratio of NH4+/NO3-, for both shoot and tuber multiplication of G.
Load more

Recommended publications
  • Boophone Disticha
    Micropropagation and pharmacological evaluation of Boophone disticha Lee Cheesman Submitted in fulfilment of the academic requirements for the degree of Doctor of Philosophy Research Centre for Plant Growth and Development School of Life Sciences University of KwaZulu-Natal, Pietermaritzburg April 2013 COLLEGE OF AGRICULTURE, ENGINEERING AND SCIENCES DECLARATION 1 – PLAGIARISM I, LEE CHEESMAN Student Number: 203502173 declare that: 1. The research contained in this thesis, except where otherwise indicated, is my original research. 2. This thesis has not been submitted for any degree or examination at any other University. 3. This thesis does not contain other persons’ data, pictures, graphs or other information, unless specifically acknowledged as being sourced from other persons. 4. This thesis does not contain other persons’ writing, unless specifically acknowledged as being sourced from other researchers. Where other written sources have been quoted, then: a. Their words have been re-written but the general information attributed to them has been referenced. b. Where their exact words have been used, then their writing has been placed in italics and inside quotation marks, and referenced. 5. This thesis does not contain text, graphics or tables copied and pasted from the internet, unless specifically acknowledged, and the source being detailed in the thesis and in the reference section. Signed at………………………………....on the.....….. day of ……......……….2013 ______________________________ SIGNATURE i STUDENT DECLARATION Micropropagation and pharmacological evaluation of Boophone disticha I, LEE CHEESMAN Student Number: 203502173 declare that: 1. The research reported in this dissertation, except where otherwise indicated is the result of my own endeavours in the Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg.
    [Show full text]
  • Autumn 2019 Beechill Bulbs Welcome to Our Autumn 2019 Collection
    Autumn 2019 Beechill Bulbs Welcome to our autumn 2019 collection. As always, bursting with colour and ideas to transform your areas into dazzling displayof warmth and vibrance at an otherwise cold and dull time of the year. Biodiversity & Sustainability This subject has gathered significant momentum now with everincreasing interest in more nature friendly varieties - Alliums,Crocus etc., as well as an increasing scrutiny on the methods ofgrowing and harvesting. This is reflected in the ongoing interest in the increasing amount of organic bulb Beechill Bulbs Ltd lines that are now available (pages 110 to 115). Ballyduff Mechanical planting Tullamore The mechanical planter has proven itself to be a worthwhile Co. Offaly companion on any suitable planting project. As a minimum damage, maximum impact method of planting it has P: +353-57-9322956 no match. Suitable for projects of 100m upwards, we’re F: +353-57-9322957 available to come on-site and give you a survey for suitability and a quote there and then. E: [email protected] Keukenhof trip W: www.bulbs.ie Our annual trip to the Keukenhof gardens has proven to be most popular as a training day in relation to the @beechillbulbs plant production and nurseries, as well as a networking opportunity, with some solid connections - even friendships @BeechillBulbs - formed over the years. beechillbulbs Weather Always a topic near the front of any gardeners mind, the Beast from the east wreaked havoc on flowering times and indeed plant health last year, followed by the drought from the south. This impacted on bulb sizes, yields and disease.
    [Show full text]
  • Hill View Rare Plants, Summer Catalogue 2011, Australia
    Summer 2011/12 Hill View Rare Plants Calochortus luteus Calochortus superbus Susan Jarick Calochortus albidus var. rubellus 400 Huon Road South Hobart Tas 7004 Ph 03 6224 0770 Summer 2011/12 400 Huon Road South Hobart Tasmania, 7004 400 Huon Road South Hobart Tasmania, 7004 Summer 2011/12 Hill View Rare Plants Ph 03 6224 0770 Ph 03 6224 0770 Hill View Rare Plants Marcus Harvey’s Hill View Rare Plants 400 Huon Road South Hobart Tasmania, 7004 Welcome to our 2011/2012 summer catalogue. We have never had so many problems in fitting the range of plants we have “on our books” into the available space! We always try and keep our lists “democratic” and balanced although at times our prejudices show and one or two groups rise to the top. This year we are offering an unprecedented range of calochortus in a multiplicity of sizes, colours and flower shapes from the charming fairy lanterns of C. albidus through to the spectacular, later-flowering mariposas with upward-facing bowl-shaped flowers in a rich tapestry of shades from canary-yellow through to lilac, lavender and purple. Counterpoised to these flashy dandies we are offering an assortment of choice muscari whose quiet charm, softer colours and Tulipa vvedenskyi Tecophilaea cyanocrocus Violacea persistent flowering make them no less effective in the winter and spring garden. Standouts among this group are the deliciously scented duo, M. muscarimi and M. macrocarpum and the striking and little known tassel-hyacith, M. weissii. While it has its devotees, many gardeners are unaware of the qualities of the large and diverse tribe of “onions”, known as alliums.
    [Show full text]
  • Isolation and Identification of Bacterial Endophytes from Crinum Macowanii Baker
    Vol. 17(33), pp. 1040-1047, 15 August, 2018 DOI: 10.5897/AJB2017.16350 Article Number: 6C0017758202 ISSN: 1684-5315 Copyright ©2018 Author(s) retain the copyright of this article African Journal of Biotechnology http://www.academicjournals.org/AJB Full Length Research Paper Isolation and identification of bacterial endophytes from Crinum macowanii Baker Rebotiloe F. Morare1*, Eunice Ubomba-Jaswa1,2 and Mahloro H. Serepa-Dlamini1 1Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, P. O. Box 17011 Doornfontein 2028, Johannesburg, South Africa. 2Water Research Commission, Lynnwood Bridge Office Park, Bloukrans Building, 4 Daventry Street, Lynnwood Manor, Pretoria, South Africa. Received 30 November, 2017; Accepted 4 July, 2018 The widespread distribution of Crinum macowanii across the African continent has entrenched the plant’s medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and Staphylococcus species C6 were isolated from fresh C. macowanii bulb and their phenotypic and genotypic profiles verified by Gram staining and 16S rRNA gene sequencing; respectively. The latter was used to construct a phylogenetic tree that showed similarities (higher than 50 bootstrap values) among the endophytic bacterial isolates. Chemical analysis of bacterial endophytes was done by extracting the crude extracts of each endophyte. Antibacterial activity of each endophyte was performed against a few selected bacterial pathogenic strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus cereus) using the disk diffusion method with Streptomycin used as a positive control.
    [Show full text]
  • Tracing History
    Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 911 Tracing History Phylogenetic, Taxonomic, and Biogeographic Research in the Colchicum Family BY ANNIKA VINNERSTEN ACTA UNIVERSITATIS UPSALIENSIS UPPSALA 2003 Dissertation presented at Uppsala University to be publicly examined in Lindahlsalen, EBC, Uppsala, Friday, December 12, 2003 at 10:00 for the degree of Doctor of Philosophy. The examination will be conducted in English. Abstract Vinnersten, A. 2003. Tracing History. Phylogenetic, Taxonomic and Biogeographic Research in the Colchicum Family. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 911. 33 pp. Uppsala. ISBN 91-554-5814-9 This thesis concerns the history and the intrafamilial delimitations of the plant family Colchicaceae. A phylogeny of 73 taxa representing all genera of Colchicaceae, except the monotypic Kuntheria, is presented. The molecular analysis based on three plastid regions—the rps16 intron, the atpB- rbcL intergenic spacer, and the trnL-F region—reveal the intrafamilial classification to be in need of revision. The two tribes Iphigenieae and Uvularieae are demonstrated to be paraphyletic. The well-known genus Colchicum is shown to be nested within Androcymbium, Onixotis constitutes a grade between Neodregea and Wurmbea, and Gloriosa is intermixed with species of Littonia. Two new tribes are described, Burchardieae and Tripladenieae, and the two tribes Colchiceae and Uvularieae are emended, leaving four tribes in the family. At generic level new combinations are made in Wurmbea and Gloriosa in order to render them monophyletic. The genus Androcymbium is paraphyletic in relation to Colchicum and the latter genus is therefore expanded.
    [Show full text]
  • The New Kirstenbosch Bulb Terrace
    - Growing indigenous Working with the seasons The new Kirstenbosch Bulb Terrace by Graham Duncan, Kirstenbosch Heavy winter rains, inadequately drained soils and insufficient winter light lev­ els experienced in many parts of Kirstenbosch preclude the display of a wide vari­ ety of our spectacular wealth of winter-growing bulbous plants in the garden itself. In addition, the depredations of molerats, and more importantly, marauding por­ cupines place further constraints on bulbs that can be displayed to the public. For these reasons the more fastidious species are cultivated under cover in the Kirstenbosch bulb nursery and displayed in containers, in season, inside the Kay Bergh Bulb House of the Botanical Society Conservatory. Although bulbous plants that are able to stand up to the rigours of general gar­ den cultivation are displayed in many parts of the garden, no section is specifical­ ly dedicated to bulbs. However, with the recent completion of the Centre for Home Gardening, an area known as the Bulb Terrace has been specifically provided for the display of both winter- and summer-growing bulbs. We hope these displays will draw attention to the many bulbous species suitable for home gardens. Passing through the Centre for Home Gardening towards the garden, the Bulb Terrace comprises eight broadly rectangular beds on either side of the sloping main bricked walkway adjacent to the new Kirstenbosch Tearoom. Four beds on each side of the walkway alternate with wooden benches. Quantities of heavy, poorly ABOVE: The dwarf Watsonia coccinea provides a brilliant splash of reddish-orange in mid-September. Photo Graham Duncan drained soil was removed from each bed.
    [Show full text]
  • Integrated Nutrient Management for Growth and Yield in Glory Lily (Gloriosa Superba L.)
    Vol. 7(43), pp. 3198-3202, 17 November, 2013 DOI: 10.5897/JMPR12.1127 ISSN 1996-0875 ©2013 Academic Journals Journal of Medicinal Plants Research http://www.academicjournals.org/JMPR Full Length Research Paper Integrated nutrient management for growth and yield in Glory Lily (Gloriosa superba L.) L. M. Gupta1*, Sandeep Kumar1, Meenakshi Gupta1 and Vikas Sharma2 1Division of Agroforestry, Faculty of Agriculture, Sher-e- Kashmir university of Agricultural Sciences and Technology, Main Campus, Chatha, Jammu (J&K) INDIA 180 009 2Division of Soil Science & Agricultural. Chemistry, Sher-e- Kashmir university of Agricultural Sciences and Technology, Main Campus, Chatha, Jammu (J&K) INDIA 180 009 Accepted 1 November, 2013 Gloriosa superba L. is an important medicinal plant species found in tropical and subtropical regions of India. Seeds and tubers contain colchicine which is used for treating gout, rheumatism and for producing polyploides. Due to diverse use, the species has been largely exploited from natural habitat leading to its threatened status. A field experiment was conducted to study the influence of organic and inorganic fertilizers on its growth and seed yield. Eight treatments including Control (T1), NPK at 120:50:75 kg/ha (recommended dose) (T2), FYM at 20 t/ha (T3), Vermicompost(VC) at 6 t/ha (T4), FYM at 10 t/ha + ½ T2 (T5), VC at 3 t/ha + ½ T2 (T6), FYM at 13.3 t/ha + ⅓T2 (T7), VC at 4 t/ha + ⅓T2 (T8) were followed to find their effect on growth and yield. The treatments were significantly different from each other and higher values were recorded for plant height (144.96 cm), number of leaves plant-1 (172.03), number of branches plant-1 (4.35), number of flowers plant-1 (30.50), number of fruits plant-1 (10.10), seed -1 -1 yield plant (4.47 g) and estimated seed yield hectare (197.50 kg) in the treatment VC at 4 t/ha + ⅓T2.
    [Show full text]
  • A Review on Phytochemical and Pharmacological Profile of Gloriosa
    Sachin Chaudhary et al. Int. Res. J. Pharm. 2019, 10 (4) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 – 8407 Review Article A REVIEW ON PHYTOCHEMICAL AND PHARMACOLOGICAL PROFILE OF GLORIOSA SUPERBA LINN Sachin Chaudhary 1, Abdel-Nasser El-Shorbagi 1, Bhawna Shridhar 2, Mandeep Kumar Gupta 2, Harish Chandra Verma 2* 1Department oF Medicinal Chemistry, College oF Pharmacy, University oF SharJah, SharJah-27272, United Arab Emirates 2Department oF Pharmaceutical Sciences, Moradabad Educational Trust Group oF Institutions, Faculty oF Pharmacy, Moradabad-244001, Uttar Pradesh, India *Corresponding Author Email: [email protected] Article Received on: 30/01/19 Approved for publication: 12/03/19 DOI: 10.7897/2230-8407.1004113 ABSTRACT The current review article target on taxonomical, phytochemical and medicinal beneFits of Gloriosa superba Linn. It is one oF the endangered species among the medicinal plants hence International Union for Conservation of Nature has placed it in ‘Red Data Book’. It is used as an analgesic, anti- inFlammatory, anti-thrombotic, anticoagulant, anticancer, antimicrobial, antiFungal, lipoXygenase inhibitor. In recent years, this plant is eXtensively utilized For the production of colchicine to treat gout. The prevalent clinical symptoms of poisoning due to ingestion of this plant are gastroenteritis, nausea, vomiting, diarrhea, dehydration and acute renal dysFunctioning. This review article illustrate the importance oF G. superba to retrieve the Future prospects. Key words: Gloriosa superba Linn, Phytochemical, Medicinal, Colchicine, Poisoning. INTRODUCTION TAXONOMIC CLASSIFICATION Gloriosa superba Linn., (Glory lily) is a medicinal plant The taXonomy oF Gloriosa superba is in the kingdom (Plantae), belonging to the Family Liliaceae. Gloriosa superba derives its order (Liliales), family (Liliaceae), genus (Gloriosa), division name Gloriosa from the word “Glorious”, which means (Magnoliophyta), class (Liliopsida), species (Superba).
    [Show full text]
  • Review of Ethnomedicinal Uses of Gloriosa Superba Plant of Nandurbar District, Maharashtra
    http://www.epitomejournals.com, Vol. 3, Issue 7, September 2017, ISSN: 2395-6968 REVIEW OF ETHNOMEDICINAL USES OF GLORIOSA SUPERBA PLANT OF NANDURBAR DISTRICT, MAHARASHTRA 1Sumia Fatima 2Jadhav Reena Girdharilal Dr.Rafiq Zakaria College for Women, Govt. Ashram Jr. College, Nawapada, Navkhanda Palace, Jubilee Park, Taluka.- Sakri, Dist.- Dhule (M.S.) India. Aurangabad, (M.S.) India. ABSTRACT Gloriosa superba is a famous Ethnomedicinal plant of Nandurbar district. Beautiful, multicolour flowers attracts the tribal of study area in the month of July - October to collect the V and L shaped underground tubers. Due to presence of Colchicines in large quantity the collection of tubers turns into commercial practice. This affects the status of the plant. Ethnomedicinal approach towards species Gloriosa superba is a small attempt to realize the ethnic glory of plant in present study area. Large number of diseases prevailing in Nandurbar district was treated with this ethno medicine. Its medicinal potential is known to tribal’s from long time and now the commercial over collection of tubers means starting of competition between pharmaceutical production of raw material based companies of other countries in our remote and tribal areas also. Gloriosa superba is widely distributed in India. But it becomes Endanger in present study area of Nandurbar district. KEYWORDS Gloriosa superba, Ethnomedicines, Nandurbar district 1 SF & JRG Impact Factor = 3.656 Dr. Pramod Ambadasrao Pawar, Editor-In-Chief ©Eijmr, All Rights Reserved. http://www.epitomejournals.com, Vol. 3, Issue 7, September 2017, ISSN: 2395-6968 RESEARCH PAPER Introduction: Gloriosa superba is known as the Kal-Kutti or Kal-lavi in the Nandurbar district.
    [Show full text]
  • Gloriosas-Apr11
    fresh focus By Bill McKinley AIFD and Bruce Wright There ’s only one word for these exotic beauties: glorious! Gloriosa Gloriosa superba THE VERY FEATURES THAT make Availability arch backward, following the At Oregon Coastal Farms near Tillamook the gloriosa lily so striking in ap - year-round petals’ path)? (www.calla-lily.com ), gloriosas can be pearance also make it a flower This is clearly not a bou - harvested from July through September (from Vase life that demands special considera - 5 to 10 days quet-maker’s flower. Typically other domestic growers, they may be available tion from designers. How do you reserved for weddings, special as early as April). The clambering vines re - work with a flower that hangs events, and dramatic floral quire a support structure to which they cling upside-down from a slender vine, its recurv - displays, the gloriosa requires space around with the tendrils that extend from the tips of ing petals pointing upward as though blown the bloom so that the flower head’s distinctive their leaves. The blooms open from the bottom back by a stiff breeze, each wavy-edged petal form can be appreciated; at the same time, the of the vine up and grow rich in color as they well separated from the others, and with five individual flower stems are relatively short, mature, so that in a red and yellow variety like prominent stamens perpendicular to the petals while longer sections of gloriosa vine require Rothschildiana, the youngest flowers may be (although, as the flower matures, they tend to external support.
    [Show full text]
  • Aβ) from South African Plants Using an Automated HPLC/SPE/HPLC Coupling System
    Original Article Biomol Ther 19(1), 90-96 (2011) Rapid Identification of Bioactive Compounds Reducing the Production of Amyloid β-Peptide (Aβ) from South African Plants Using an Automated HPLC/SPE/HPLC Coupling System Hak Cheol Kwon1, Jin Wook Cha1, Jin-Soo Park1, Yoon Sun Chun2, Nivan Moodley3, Vinesh J. Maharaj3, Sung Hee Youn2, Sungkwon Chung2,* and Hyun Ok Yang1,* 1Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, 2Department of Physiology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea, 3Biosciences, CSIR, RSA, PO Box 395, Pretoria, 0001, South Africa Abstract Automated HPLC/SPE/HPLC coupling experiments using the Sepbox system allowed the rapid identifi cation of four bioactive principles reducing the production of amyloid β-peptide (Aβ) from two South African plants, Euclea crispa subsp. crispa and Cri- num macowanii. The structures of biologically active compounds isolated from the methanol extract of Euclea crispa subsp. crispa were assigned as 3-oxo-oleanolic acid (1) and natalenone (2) based on their NMR and MS data, while lycorine (3) and hamayne (4) were isolated from the dichloromethane-methanol (1:1) extract of Crinum macowanii. These compounds were shown to inhibit the production of Aβ from HeLa cells stably expressing Swedish mutant form of amyloid precursor protein (APPsw). Key Words: HPLC/SPE/HPLC, Alzheimer’s disease, Amyloid β-peptide, Euclea crispa subsp. crispa, Crinum macowanii INTRODUCTION of many novel therapeutic agents (John, 2009). However, the classical bioactivity-guided fractionation for the purifi cation As one of the most serious health problems worldwide, Al- and identifi cation of biologically active compounds from plants zheimer’s disease (AD) is the most common form of age-relat- extracts can be tedious process with long time frames (Clark- ed dementia (Lim et al., 2006).
    [Show full text]
  • High-Performance Liquid Chromatographic Determination of Colchicine in Pharmaceutical Formulations and Biological Fluids
    Innovare International Journal of Pharmacy and Pharmaceutical Sciences Academic Sciences ISSN- 0975-1491 Vol 6, Issue 6, 2014 Original Article HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF COLCHICINE IN PHARMACEUTICAL FORMULATIONS AND BIOLOGICAL FLUIDS BABU GIRIYA GOWDA* Department of Chemistry, Maharani’s Science College for Women, Bangalore 560001, India. Email: [email protected] Received: 24 Apr 2014 Revised and Accepted: 23 May 2014 ABSTRACT Objective: A simple, rapid, precise and accurate high performance liquid chromatography (HPLC) method has been developed for the estimation of colchicine (COLC) in bulk drug, pharmaceutical formulations and biological fluids. Methods: The developed HPLC method involves the using of a mixture of acetonitrile : methanol : water (32:48:20 v/v) as a mobile phase. The pH of the mobile phase was adjusted to 5.2 with phosphoric acid. A CLC C18 column . The flow rate of the mobile phase was set to 1.2 ml/min. Injection volume was set at 20 l and the detection of the analyte was done at 254 nm. (5 μm, 25 cm x 4.6 mm i.d.) was used for the elution Results: The linear regression analysis data for calibration curve μshowed a good relationship with correlation coefficient of 0.9997. The concentration range was 7-130 g/ml. The percentage recovery of COLC was found to be 99.87 %. The limits of detection and quantification are also reported. This selective method is found to be accurate, precise and effectively used for the determination of COLC in various pharmaceutical formulations and biological fluidsμ with better chromatographic conditions. Conclusions: The method was successfully applied to the assay of COLC in pharmaceutical formulations and biological fluids and the results were statistically compared with those of the reference method by applying Student’s t-test and F-test.
    [Show full text]