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states withinthoseregionsadaptedthe amounts. EachEPA Regionandthe oftoxicpollutantsin the CleanWater Act’s prohibitionofthe Agency (USEPA) asameanstoenforce United StatesEnvironmentalProtection (WET) testsweredevelopedbythe During the1970’s WholeEffluent terrestrial invertebratespecies. and sedimentmaterialswithplant Bioassay testscanalsobeappliedtosoil lethal orsub-lethaleffects areobserved. of anaqueoussolutiontodetermineif are subjectedtovaryingconcentrations on aquaticorganisms. These studies theadverseeffects ofchemicals Aquatic isananalysiswhich Aquatic Toxicology toxicity issues. solving problemsforthose whohave meet theircompliancerequirements and We havealonghistoryofhelpingclients of theCleanWater Act (CWA) since 1987. services towastewaterclientsinsupport experts havebeenprovidingbioassay laboratories intheMid-Atlantic,our As oneofthepremieraquatictoxicology toxicology testinginplace. is requiredtohaveaprogramofaquatic Today everystateandterritoryoftheUS EPA protocolstomeetthoseobjectives. Environment Testing US? Why ChooseEurofins • • • • • healthy organismsfortesting. a continuousandreliablesupplyof cultured withinourfacility. This ensures of theorganismswetestwithare In-house Cultures: discharge compliancerequirements. interpret stateandfederalwaste assistance inordertoeffectively Compliance: Permit ReviewandRegulatory NELAP program. in statesthatdonotparticipatethe we maintaincertificationsorapprovals in theNELAP program.Inaddition, perform testsinanystateparticipating laboratory, wehavetheabilityto Credentials: help determineandsolvetoxicityissues. credible scientistscanbecalleduponto of experience,ourknowledgeableand Consulting: less thanone week. designed reports, our TAT istypically with theselayersofreview andcustom to calculateendpointsprecisely. Even employ thelateststatistical software and regulatoryreporting.We also to meetclient’s requirementsforinternal the client.Reportsarecustomdesigned QC checkspriortobeingsubmitted data goesthroughpeerandinternalQA/ Fast and Accurate Results: Withmorethan25years As aNELAC-accredited We areabletoprovide The majority All ofour

9555 0516 Our Capabilities • Field sampling We offer a broad range of aquatic • Completely customized testing toxicology testing including the design of All testing is conducted in our state- client-specific test protocols required to of-the-art facility designed specifically satisfy CWA specifications and other non- for aquatic toxicology testing. All of our wastewater needs. aquatic toxicology tests are performed Our testing capabilities include: in accordance with USEPA methods. • Acute and Chronic Whole If required, customized protocols are Toxicity testing with freshwater and employed for non-wastewater sources marine species to comply with other regulatory agency requirements. • /Soil Assays • Toxicity Identification Evaluations Our culture laboratory consists of systems (TIEs) designed for the purpose of breeding healthy organisms with genetic traceability. • Chlorophyll a analysis Monthly Standard Reference • Water Effect Ratios (WERs) (SRT) tests are performed on all organisms • Pure compound testing. cultured in house. Concurrent SRT tests are • surveys (biological/chemical) performed on organisms brought in from for impact and outside culture laboratories. All SRT data is studies graphed and evaluated for consistency.

Methods for Routine Bioassay Testing Species Method Pimephales promelas (Fathead Minnow) EPA 1000.0; 2000.0

Ceriodaphnia dubia (Water Flea) EPA 1002.0; 2002.0

Selenastrum capricornutum (Microalga) EPA 1003 Cyprinodon variegatus (Sheepshead Minnow) EPA 1004; 2004.0

Menidia beryllina (Inland Silverside) EPA 1006; 2006.0

Mysidopsis bahia (Mysid Shrimp) EPA 1007; 2007.0

Cyprinella leedsi (Bannerfin Shiner) EPA 2000.0

Daphnia pulex (Water Flea) EPA 2021.0

Hyalella azteca EPA 100.1 / 100.4 Chironomus dilutus (formerly tentans) EPA 100.2 / 100.5

Lumbriculus variegatus EPA 100.3 Other specialized testing on demand

Eurofins Environment Testing US - Centers of Excellence

Eurofins Air Toxics Eurofins Calscience Eurofins Eaton Eurofins Frontier Eurofins Lancaster Eurofins QC Eurofins Spectrum Folsom, CA Garden Grove, CA Analytical Global Sciences Laboratories Southampton, PA Analytical 800-985-5955 714-895-5494 Monrovia, CA Bothell, WA Environmental 215-355-3900 Agawam, MA 626-386-1100 425-686-1996 Lancaster, PA Horsham, PA 413-789-9018 South Bend, IN 717-656-2300 215-355-3900 North Kingston, RI 574-233-4777 401-732-3400 www.EurofinsUS.com/Env