DOCSLIB.ORG

Ameyamaea Chiangmaiensis Gen. Nov., Sp. Nov., an Acetic Acid Bacterium in the -Proteobacteria

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Ameyamaea Chiangmaiensis Gen. Nov., Sp. Nov., an Acetic Acid Bacterium in the -Proteobacteria 90070 (379) Biosci. Biotechnol. Biochem., 73, 90070-1–7, 2009 Ameyamaea chiangmaiensis gen. nov., sp. nov., an Acetic Acid Bacterium in the -Proteobacteria Pattaraporn YUKPHAN,1 Taweesak MALIMAS,1 Yuki MURAMATSU,2 Mai TAKAHASHI,2 Mika KANEYASU,2 Wanchern POTACHAROEN,1 Somboon TANASUPAWAT,3 Yasuyoshi NAKAGAWA,2 Koei HAMANA,4 Yasutaka TAHARA,5 Ken-ichiro SUZUKI,2 y Morakot TANTICHAROEN,1 and Yuzo YAMADA1; ,* 1BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand 2Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kisarazu 292-0818, Japan 3Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 4School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi 371-8514, Japan 5Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan Received January 27, 2009; Accepted July 8, 2009; Online Publication, October 7, 2009 [doi:10.1271/bbb.90070]Advance View Two isolates, AC04T and AC05, were isolated from Key words: Ameyamaea chiagmaiensis gen. nov., sp. the flowers of red ginger collected in Chiang Mai, nov.; acetic acid bacteria; 16S rRNA gene Thailand. In phylogenetic trees based on 16S rRNA sequences; 16S rRNA gene restriction anal- gene sequences, the two isolates were included within a ysis; Acetobacteraceae lineage comprised of the genera Acidomonas, Glucona- cetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, In acetic acid bacteria, several new genera have been Granulibacter, and Tanticharoenia, and they formed an reported for strains isolated from isolation sources independent cluster along with the type strain of obtained in Southeast Asia. The first was the genus Tanticharoenia sakaeratensis. The calculated pair-wise Asaia Yamada etProofs al. 2000, with a single species, Asaia sequence similarities of isolate AC04T were 97.8–92.5% bogorensis Yamada et al. 2000.2) Asaia siamensis to the type strains of the type species of the 11 genera Katsura et al. 2001,3) Asaia krungthepensis Yukphan of acetic acid bacteria. The DNA base composition et al. 2004,4) and Asaia lannensis Malimas et al. 20085,6) was 66.0–66.1 mol % G+C with a range of 0.1 mol %. were additionally described. The second and the third A single-stranded, labeled DNA from isolate AC04T were the genus Kozakia Lisdiyanti et al. 20027) and the presented levels of DNA-DNA hybridization of 100, 85, genus Neoasaia Yukphan et al. 2006,8,9) with Kozakia 4, and 3% respectively to DNAs from isolates AC04T baliensis Lisdiyanti et al. 2002 and Neoasaia chiang- and AC05 and the type strains of Tanticharoenia maiensis Yukphan et al. 2006 respectively. The fourth sakaeratensis and Gluconacetobacter liquefaciens. The was recently described as the genus Tanticharoenia two isolates were unique morphologically in polar Yukphan et al. 200810,11) with Tanticharoenia sakaer- flagellation and physiologically in intense acetate oxida- atensis Yukphan et al. 2008. tion to carbon dioxide and water and weak lactate In the course of studies on microbial diversity in the oxidation. The intensity in acetate oxidation almost natural environment of Thailand, a chance to isolate two equaled that of the type strain of Acetobacter aceti. The types of unique acetic acid bacteria was given on the two isolates had Q-10. Isolate AC04T was discriminated same day, September 20, 2002, from the same kind of from the type strains of the type species of the 11 genera isolation sources obtained at the same place, the campus by 16S rRNA gene restriction analysis using restriction of Chiang Mai University, Chiang Mai, in the northern endonucleases TaqI and Hin6I. The unique phylogenet- district of Thailand. One was the type strain of Neoasaia ic, genetic, morphological, physiological, and biochem- chiagmaiensis,8) and the others were two isolates ical characteristics obtained indicate that the two discussed below. isolates can be classified into a separate genus, and This paper proposes Ameyamaea chiangmaiensis Ameyamaea chiangmaiensis gen. nov., sp. nov. is pro- gen. nov., sp. nov. for the two isolates accommodated posed. The type strain is isolate AC04T (¼ BCC 15744T, to the family Acetobacteraceae Gillis and De Ley ¼ NBRC 103196T), which has a DNA G+C content of 1980.12) 66.0 mol %. y To whom correspondence should be addressed. Tel/Fax: +81-54-635-2316; E-mail: [email protected] * JICA Senior Overseas Volunteer, Japan International Cooperation Agency (JICA), Shibuya-ku, Tokyo 151-8558, Japan; Professor Emeritus, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan The DDBJ accession numbers of the 16S rRNA gene sequences are AB303366 and AB303367 for isolates AC04T and AC05 respectively.1) 90070-2 P. YUKPHAN et al. Materials and Methods ever, the phylogenetic distance between the isolates and the type strain of the Tanticharoenia species was much Isolates AC04T and AC05 were examined. They were isolated from longer than that between the type strains of the Asaia the flowers of red ginger (khing daeng in Thai, Alpinia purpurea) and Swaminathania species. In addition, the type strains collected in Chiang Mai, Thailand by an enrichment culture approach of Kozakia baliensis and Neoasaia chiangmaiensis gave using a sorbitol medium, which contained 2.0% D-sorbitol, 0.3% peptone, and 0.3% yeast extract (all by w/v) and was adjusted to a similar cluster, with a bootstrap value of 85%, to that pH 3.5, among the four media used.2–5,7,8,10,13,14) Acetobacter aceti of the isolates and the type strain of the Tanticharoenia NBRC 14818T (NBRC, NITE Biological Resource Center, Department species. In both clusters, the phylogenetic distances were of Biotechnology, National Institute of Technology and Evaluation, almost identical to each other. The cluster comprised of Kisarazu, Chiba, Japan), Gluconobacter oxydans NBRC 14819T, the two isolates and the type strain of the Tanticharoenia T Acidomonas methanolica NRIC 0498 (NRIC, NODAI Research species was independently connected to a large cluster Institute Culture Collection Center, Tokyo University of Agriculture, of the genera Asaia, Swaminathania, Kozakia, Neoasaia, Tokyo, Japan), Gluconacetobacter liquefaciens NBRC 12388T, Asaia bogorensis NBRC 16594T, Kozakia baliensis NBRC 16664T, Swami- Acidomonas, Granulibacter, and Gluconacetobacter, nathania salitolerans LMG 21291T (LMG, Laboratorium voor Micro- with a bootstrap value of 85%. biologie, Universiteit Gent, Ghent, Belgium), Saccharibacter floricola In a phylogenetic tree constructed by the maximum BCC 16445T (BCC, BIOTEC Culture Collection, National Center parsimony method, the cluster of the two isolates and the for Genetic Engineering and Biotechnology, National Science and type strain of the Tanticharoenia species had a bootstrap Technology Development Agency, Pathumthani, Thailand), Neoasaia value of 75%, connected itself with the cluster com- chiangmaiensis T Granulibacter bethesdensis BCC 15763 , ATCC BAA prised of the genera Kozakia, Neoasaia, Acidomonas, 1260T (ATCC, American Type Culture Collection, Rockville, MD, USA), and Tanticharoenia sakaeratensis BCC 15772T were used as Granulibacter, and Gluconacetobacter, with a bootstrap reference strains. value below 50%, and formed a large cluster together PCR amplification of the 16S rRNA genes was done, and the with the cluster of the genera Asaia and Swaminathania, amplified 16S rRNA genes were sequenced and analyzed as described with a bootstrap value of 53% (Fig. 1B). The phyloge- previously.4,8,15) Multiple sequence alignment was done with the netic distance between the two isolates and the type 16) programAdvance CLUSTAL X (version 1.8). Alignment gaps and View unidenti- Tanticharoenia strain of the species was almost identical fied bases were eliminated. Distance matrices for the aligned sequences were calculated by the two-parameter method of Kimura.17) Phyloge- to that between the type strains of the Kozakia and netic trees were constructed for 1,374 bases by the neighbor-joining,18) Neosasaia species and much longer than that between maximum parsimony,19) and maximum likelihood20) methods. The the type strains of the Asaia and Swaminathania species, robustness of individual branches was estimated by bootstrapping21) as found in the phylogenetic tree constructed by the with the program MEGA (version 4.0).22) Bootstrap values below 50% neighbor-joining method. were not shown. In constructing the phylogenetic tree by the maximum In the phylogenetic tree constructed by the maximum likelihood method, the program PHYLIP (version 3.6; J. Felsenstein, likelihood method, the cluster of the two isolates and the University of Washington) was used. Pair-wise sequence similarities were calculated in 16S rRNA gene sequences of 1,390 bases. type strain of the Tanticharoenia species was connected A computerized 16S rRNA gene restriction analysis was done using to a cluster comprised of the genera Gluconacetobacter, NEBcutter (version 2.0; New England BioLabs, Ipswich, MA, USA) Acidomonas, andProofsGranulibacter with a bootstrap value for isolate AC04T and the type strains of the type species of the 11 below 50%, and the resulting cluster formed a large genera of acetic acid bacteria. The 16S rRNA gene PCR products of cluster with the cluster of the genera Asaia, Swamina- isolate AC04T and the type strains were prepared and digested with 23) thania, Kozakia, and Neoasaia, with a bootstrap value
Load more

Recommended publications
  • Identification of Strains Isolated in Thailand and Assigned to the Genera Kozakia and Swaminathania
    JOURNAL OF CULTURE COLLECTIONS Volume 6, 2008-2009, pp. 61-68 IDENTIFICATION OF STRAINS ISOLATED IN THAILAND AND ASSIGNED TO THE GENERA KOZAKIA AND SWAMINATHANIA Jintana Kommanee1, Somboon Tanasupawat1,*, Ancharida Akaracharanya2, Taweesak Malimas3, Pattaraporn Yukphan3, Yuki Muramatsu4, Yasuyoshi Nakagawa4 and Yuzo Yamada3,† 1Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; 2Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; 3BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand; 4Biological Resource Center, Department of Biotechnology, National Institute of Technology and Evaluation, Kisarazu 292-0818, Japan; †JICA Senior Overseas Volunteer, Japan International Cooperation Agency, Shibuya-ku, Tokyo 151-8558, Japan; Professor Emeritus, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan *Corresponding author, e-mail: [email protected] Summary Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora („khem” in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5.
    [Show full text]
  • Ameyamaea Chiangmaiensis Gen. Nov., Sp. Nov., an Acetic Acid Bacterium in the -Proteobacteria
    Biosci. Biotechnol. Biochem., 73 (10), 2156–2162, 2009 Ameyamaea chiangmaiensis gen. nov., sp. nov., an Acetic Acid Bacterium in the -Proteobacteria Pattaraporn YUKPHAN,1 Taweesak MALIMAS,1 Yuki MURAMATSU,2 Mai TAKAHASHI,2 Mika KANEYASU,2 Wanchern POTACHAROEN,1 Somboon TANASUPAWAT,3 Yasuyoshi NAKAGAWA,2 Koei HAMANA,4 Yasutaka TAHARA,5 Ken-ichiro SUZUKI,2 y Morakot TANTICHAROEN,1 and Yuzo YAMADA1; ,* 1BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand 2Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kisarazu 292-0818, Japan 3Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 4School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi 371-8514, Japan 5Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan Received January 27, 2009; Accepted July 8, 2009; Online Publication, October 7, 2009 [doi:10.1271/bbb.90070] Two isolates, AC04T and AC05, were isolated from Key words: Ameyamaea chiagmaiensis gen. nov., sp. the flowers of red ginger collected in Chiang Mai, nov.; acetic acid bacteria; 16S rRNA gene Thailand. In phylogenetic trees based on 16S rRNA sequences; 16S rRNA gene restriction anal- gene sequences, the two isolates were included within a ysis; Acetobacteraceae lineage comprised of the genera Acidomonas, Glucona- cetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, In acetic acid bacteria, several new genera have been Granulibacter, and Tanticharoenia, and they formed an reported for strains isolated from isolation sources independent cluster along with the type strain of obtained in Southeast Asia. The first was the genus Tanticharoenia sakaeratensis.
    [Show full text]
  • Dissection of Exopolysaccharide Biosynthesis in Kozakia Baliensis Julia U
    Brandt et al. Microb Cell Fact (2016) 15:170 DOI 10.1186/s12934-016-0572-x Microbial Cell Factories RESEARCH Open Access Dissection of exopolysaccharide biosynthesis in Kozakia baliensis Julia U. Brandt, Frank Jakob*, Jürgen Behr, Andreas J. Geissler and Rudi F. Vogel Abstract Background: Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan. Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose. Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose- deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources. This indicated that both Kozakia strains additionally produce new classes of so far not characterized EPS. Results: By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified. As expected, complete ORFs coding for levansucrases could be detected in both Kozakia strains. In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680. Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthe- sis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus. A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
    [Show full text]
  • Kozakia Baliensis Gen. Nov., Sp. Nov., a Novel Acetic Acid Bacterium in The
    International Journal of Systematic and Evolutionary Microbiology (2002), 52, 813–818 DOI: 10.1099/ijs.0.01982-0 Kozakia baliensis gen. nov., sp. nov., a novel NOTE acetic acid bacterium in the α-Proteobacteria 1 Laboratory of General and Puspita Lisdiyanti,1 Hiroko Kawasaki,2 Yantyati Widyastuti,3 Applied Microbiology, 3 2 1 1 Department of Applied Susono Saono, Tatsuji Seki, Yuzo Yamada, † Tai Uchimura Biology and Chemistry, and Kazuo Komagata1 Faculty of Applied Bioscience, Tokyo University of Agriculture, Author for correspondence: Yuzo Yamada. Tel\Fax: j81 54 635 2316. 1-1-1 Sakuragaoka, e-mail: yamada-yuzo!mub.biglobe.ne.jp Setagaya-ku, Tokyo 156- 8502, Japan 2 The International Center Four bacterial strains were isolated from palm brown sugar and ragi collected for Biotechnology, Osaka in Bali and Yogyakarta, Indonesia, by an enrichment culture approach for University, 2-1 Yamadaoka, Suita, Osaka 565-0871, acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences Japan showed that the four isolates constituted a cluster separate from the genera 3 Research and Development Acetobacter, Gluconobacter, Acidomonas, Gluconacetobacter and Asaia with a Centre for Biotechnology, high bootstrap value in a phylogenetic tree. The isolates had high values of Indonesian Institute of DNA–DNA similarity (78–100%) between one another and low values of the Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong similarity (7–25%) to the type strains of Acetobacter aceti, Gluconobacter 16911, Indonesia oxydans, Gluconacetobacter liquefaciens and Asaia bogorensis. The DNA base composition of the isolates ranged from 568to572 mol% GMC with a range of 04 mol%. The major quinone was Q-10.
    [Show full text]
  • Open NAL Thesis V6.Pdf
    The Pennsylvania State University The Graduate School Department of Civil and Environmental Engineering CLASSIFICATION OF POLYPHOSPHATE-ACCUMULATING BACTERIA IN BENTHIC BIOFILMS A Thesis in Environmental Engineering by Nicholas Locke 2015 Nicholas Locke Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science August 2015 ii The thesis of Nicholas Locke was reviewed and approved* by the following: John Regan Professor of Environmental Engineering Thesis Advisor William Burgos Professor of Environmental Engineering Chair of Civil and Environmental Engineering Graduate Programs Anthony Buda Adjunct Assistant Professor of Ecosystem Science and Management *Signatures are on file in the Graduate School iii ABSTRACT Polyphosphate accumulating organisms (PAOs) are microorganisms known to store excess phosphorus (P) as polyphosphate (poly-P) in environments subject to alternating aerobic and anaerobic conditions. There has been considerable research on PAOs in biological wastewater treatment systems, but very little investigation of these microbes in freshwater systems. We hypothesize that putative PAOs in benthic biofilms of shallow streams where daily light cycles induce alternating aerobic and anaerobic conditions are similar to those found in EBPR. To test this hypothesis, cells with poly-P inclusions were isolated, classified, and described. Eight benthic biofilms taken from a first-order stream in Mahantango Creek Watershed (Klingerstown, PA) represented high and low P loadings from a series of four flumes and were found to contain 0.39 - 6.19% PAOs. A second set of eight benthic biofilms from locations selected by Carrick and Price (2011) were from third- order streams in Pennsylvania and contained 11-48% putative PAOs based on flow cytometry particle counts.
    [Show full text]
  • Swaminathania Salitolerans Gen. Nov., Sp. Nov., a Salt-Tolerant, Nitrogen-fixing and Phosphate-Solubilizing Bacterium from Wild Rice (Porteresia Coarctata Tateoka)
    International Journal of Systematic and Evolutionary Microbiology (2004), 54, 1185–1190 DOI 10.1099/ijs.0.02817-0 Swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (Porteresia coarctata Tateoka) P. Loganathan and Sudha Nair Correspondence M. S. Swaminathan Research Foundation, 111 Cross St, Tharamani Institutional Area, Chennai, Sudha Nair Madras 600 113, India [email protected] A novel species, Swaminathania salitolerans gen. nov., sp. nov., was isolated from the rhizosphere, roots and stems of salt-tolerant, mangrove-associated wild rice (Porteresia coarctata Tateoka) using nitrogen-free, semi-solid LGI medium at pH 5?5. Strains were Gram-negative, rod-shaped and motile with peritrichous flagella. The strains grew well in the presence of 0?35 % acetic acid, 3 % NaCl and 1 % KNO3, and produced acid from L-arabinose, D-glucose, glycerol, ethanol, D-mannose, D-galactose and sorbitol. They oxidized ethanol and grew well on mannitol and glutamate agar. The fatty acids 18 : 1v7c/v9t/v12t and 19 : 0cyclo v8c constituted 30?41 and 11?80 % total fatty acids, respectively, whereas 13 : 1 AT 12–13 was found at 0?53 %. DNA G+C content was 57?6–59?9 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that these strains were related to the genera Acidomonas, Asaia, Acetobacter, Gluconacetobacter, Gluconobacter and Kozakia in the Acetobacteraceae. Isolates were able to fix nitrogen and solubilized phosphate in the presence of NaCl. Based on overall analysis of the tests and comparison with the characteristics of members of the Acetobacteraceae, a novel genus and species is proposed for these isolates, Swaminathania salitolerans gen.
    [Show full text]
  • Novel Fructans from Acetic Acid Bacteria
    TECHNISCHE UNIVERSITÄT MÜNCHEN Lehrstuhl für Technische Mikrobiologie Novel fructans from acetic acid bacteria Frank Jakob Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.-Prof. Dr. S. Scherer Prüfer der Dissertation: 1. Univ.-Prof. Dr. R. F. Vogel 2. Univ.-Prof. Dr. W. Liebl 3. apl. Prof. Dr. P. Köhler Die Dissertation wurde am 23.01.2014 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 15.04.2014 angenommen. VORWORT Die vorliegende Arbeit wurde durch Fördermittel des Bundesministeriums für Ernährung, Landwirtschaft und Verbraucherschutz (BMELV) über die Bundesanstalt für Landwirtschaft und Ernährung (BLE) unterstützt (Projekt 28-1-63.001-07). Mein besonderer Dank gilt meinem Doktorvater Prof. Dr. Rudi F. Vogel für die Möglichkeit, diese Dissertation an seinem Institut durchzuführen. Zudem möchte ich mich für seine konstruktiven Anregungen zu dieser Arbeit, sein entgegengebrachtes Vertrauen, seinen ständigen Einsatz für meine Weiterbeschäftigung an seinem Institut und für seine Unterstützung, mich wissenschaftlich weiter entwickeln zu können, bedanken. Mein außerordentlicher Dank gilt ihm außerdem für sein entgegengebrachtes Verständnis in schwierigen Phasen. Bei Dr. Daniel Meißner und Dr. Susanne Kaditzky möchte ich mich für die hilfreiche und angenehme Betreuung und bei Maria Hermann für die gute Zusammenarbeit im Projekt bedanken. Mein besonderer Dank gilt zudem Stefan Steger für die Durchführung von Backversuchen. Bei Dr. Andre Pfaff und Dr. Ramon Novoa-Carballal möchte ich mich für die entspannte Kooperation, die Durchführung von NMR-Messungen und die Bereitstellung von aufgenommenen Spektren bedanken.
    [Show full text]
  • Microbiology of Vinegar: from Isolation, Phenetic Characterization and Detection of Acetic Acid Bacteria to Microbial Profiling of an Industrial Production
    Microbiology of Vinegar: from Isolation, Phenetic Characterization and Detection of Acetic Acid Bacteria to Microbial Profiling of an Industrial Production João Nuno Serôdio de Melo Thesis to obtain the Master of Science Degree in Microbiology Supervisor(s): Prof. Rogério Paulo de Andrade Tenreiro Prof. Nuno Gonçalo Pereira Mira Examination Committee: Chairperson: Prof. Jorge Humberto Gomes Leitão Supervisor: Prof. Rogério Paulo de Andrade Tenreiro Member of the Committee: Prof. Rodrigo da Silva Costa December, 2016 Acknowledgements I would like to express my sincere gratitude to my supervisor, Professor Rogério Tenreiro, for giving me this opportunity and for all his support and guidance throughout the last year. I have truly learned immensely working with you. Also, I would like to show appreciation to my supervisor from IST, Professor Nuno Mira, for his support along the development of this thesis. I would also like to thank Professor Ana Tenreiro for all her time and help, especially with the flow cytometry assays. Additionally, I’d like to thank Professor Lélia Chambel for her help with the Bionumerics software. I would like to thank Filipa Antunes, from the Lab Bugworkers | M&B-BioISI, for the remarkable organization of the lab and for all her help. I would like to express my gratitude to Mendes Gonçalves, S.A. for the opportunity to carry out this thesis and for providing support during the whole course of this project. Special thanks to Cristiano Roussado for always being available. Also, a big thank you to my colleagues and friends, Catarina, Sofia, Tatiana, Ana, Joana, Cláudia, Mariana, Inês and Pedro for their friendship, support and for this enjoyable year, especially to Ana Marta Lourenço for her enthusiastic Gram stainings.
    [Show full text]
  • Tanticharoenia Sakaeratensis Gen. Nov., Sp. Nov., a New Osmotolerant Acetic Acid Bacterium in the -Proteobacteria
    70319 (090) Biosci. Biotechnol. Biochem., 72, 70319-1–5, 2008 Tanticharoenia sakaeratensis gen. nov., sp. nov., a New Osmotolerant Acetic Acid Bacterium in the -Proteobacteria Pattaraporn YUKPHAN,1 Taweesak MALIMAS,1 Yuki MURAMATSU,2 Mai TAKAHASHI,2 Mika KANEYASU,2 Somboon TANASUPAWAT,3 Yasuyoshi NAKAGAWA,2 Ken-ichiro SUZUKI,2 y Wanchern POTACHAROEN,1 and Yuzo YAMADA1; ,* 1BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand 2Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kisarazu 292-0818, Japan 3Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand Received May 22, 2007; Accepted November 19, 2007; Online Publication, March 7, 2008 [doi:10.1271/bbb.70319] AdvanceTanticharoenia sakaeratensis gen. nov., sp. View nov. is 1980,1) 10 genera of acetic acid bacteria are presently proposed for three strains isolated from soil collected in recognized: Acetobacter Beijerinck 1898,2) Glucono- Thailand. The three strains, AC37T, AC38, and AC39, bacter Asai 1935,2) Acidomonas Urakami et al. 1989,3,4) were included within a lineage comprising the genera Gluconacetobacter Yamada et al. 1998,5,6) Asaia Asaia, Kozakia, Swaminathania, Neoasaia, Acetobacter, Yamada et al. 2000,7) Kozakia Lisdiyanti et al. 2002,8) Gluconobacter, and Saccharibacter in a phylogenetic tree Swaminathania Loganathan and Nair 2004,9) Sacchari- based on 16S rRNA gene sequences, but formed a quite bacter Jojima et al. 2004,10) Neoasaia Yukphan et al. different, independent cluster. Pair-wise sequence sim- 2006,11,12) and Granulibacter Greenberg et al. 2006.13) ilarities of strain AC37T were 96.5–92.1% to the type Of these 10, the genera Asaia, Kozakia, and Neoasaia strains of Acetobacter aceti, Gluconobacter oxydans, were described forProofs strains isolated from isolation sources Acidomonas methanolica, Gluconacetobacter liquefa- collected in Thailand and Indonesia, Southeast Asia.
    [Show full text]
  • Multi-Marker Molecular Characterization of Ciliates and Their Endosymbionts
    Facoltà di Scienze Matematiche, Fisiche e Naturali Corso di Laurea Magistrale in Conservazione ed Evoluzione Tesi di Laurea Magistrale Multi-marker molecular characterization of ciliates and their endosymbionts Candidato Vittorio Boscaro Relatore Dott. Giulio Petroni Correlatori Prof. Alberto Castelli Prof. Sergei Fokin Anno Accademico 2010/2011 "!#$%&'()*+&$(%#!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!, "!"!#+$-$.&/0#.%)#&1/$'#/%)(0234$(%&0#!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!5 !"!"!"#$%&'#(%)*$#%+#$,&-.%$.$#""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""/ !"!"0"#1'+')23#4'256)'$#2+*#$,$5'&25.7$#%4#7.3.25'$#""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""8 !"!"9"#'7%3%1,#%4#7.3.25'$#""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""": !"!"/"#-275').23#'+*%$,&-.%+5$#%4#7.3.25'$#"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""; "!6!#3(-/+*-.'#3.'7/'0#!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!"6 !"0"!"#5<'#&635.=&2)>')#7<2)275').?25.%+#"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""!0 !"0"0"#+673'2)#))+2#1'+'#$'@6'+7'$#""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""!8 !"0"9"#7%A!#1'+'#$'@6'+7'#""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""!B
    [Show full text]
  • Tistrella Mobilis Gen. Nov., Sp. Nov., a Novel Polyhydroxyalkanoate-Producing Bacterium Belonging to Α-Proteobacteria
    J. Gen. Appl. Microbiol., 48, 335–343 (2002) Full Paper Tistrella mobilis gen. nov., sp. nov., a novel polyhydroxyalkanoate- producing bacterium belonging to a-Proteobacteria Bin-Hai Shi, Vullapa Arunpairojana,1 S. Palakawong,1 and Akira Yokota* Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113–0032, Japan 1 Thailand Institute of Scientific and Technological Research, 196, Phahonyothin Road, Chatuchak, Bangkok 10900, Thailand (Received October 2, 2001; Accepted November 13, 2002) Strain IAM 14872, isolated from wastewater in Thailand, is capable of producing polyhydrox- yalkanoate. This bacterium is Gram-negative, rod-shaped, strictly aerobic and highly motile with a single polar flagellum. Both oxidase and catalase activities are positive. The G؉C content of DNA is 67.5% and Q-10 is the major quinone. The major cellular fatty acids are C18:1w7c, 2-OH C18:0 and 3-OH C14:0. On the basis of the 16S rDNA sequence analysis and phenotypic properties, it is proposed that the strain IAM 14872 be classified in a new genus as Tistrella mobilis gen. .(TISTR 1108T؍) nov., sp. nov. The type strain is IAM 14872T Key Words——PHA and polyhydroxyalkanoate; a-Proteobacteria; 16S rDNA; taxonomy; Tistrella mo- bilis gen. nov., sp. nov. Introduction used for the microbial transformation of carbohydrate feedstock via PHA into chiral depolymerization prod- Polyhydroxyalkanoates (PHAs) are known to be pro- ucts (Seebach and Zuger, 1985) or small-molecule or- duced as intracellular granules by a variety of bacteria, ganic chemicals by pyrolysis (Anderson and Dawes, such as Alcaligenes eutrophus, Pseudomonas oleovo- 1990). So far, these biodegradable PHAs, however, rans, Rhodospirillum rubrum, etc.
    [Show full text]
  • 16S Rrna Gene Sequences Analysis of Acetic Acid Bacteria Isolated from Thailand
    Microbiol. Cult. Coll. 2（1）：135 ─ 20, 2009 16S rRNA gene sequences analysis of acetic acid bacteria isolated from Thailand Yuki Muramatsu1)*, Pattaraporn Yukphan2), Mai Takahashi1), Mika Kaneyasu1), Taweesak Malimas2), Wanchern Potacharoen2), Yuzo Yamada2), Yasuyoshi Nakagawa1), Morakot Tanticharoen2) and Ken-ichiro Suzuki1) 1) Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan 2) BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC) Pathumthani 12120, Thailand We determined the 16S rRNA gene sequences of 302 strains of acetic acid bacteria isolated from Thailand. The isolates were divided into 35 sequence groups based on differences in 16S rRNA gene sequences. A phylogenetic tree constructed from these sequences showed that 5 strains should be classified into new genera in the family Acetobacteraceae. The other 297 strains were assigned to 33 sequence groups of 4 known genera Acetobacter, Asaia, Gluconacetobacter, and Gluconobacter. Seventeen strains belonging to Acetobacter were divided into 8 sequence groups (AB1-AB8). Seven groups except for AB2 were closely related to known Acetobacter species. AB2 was remote from known Acetobacter species. We sorted 150 strains of Asaia into 11 sequence groups (AS1-AS11). AS11 was distinct from all known Asaia species. Nine strains assigned to Gluconacetobacter showed 100% sequence similarity to Gluconacetobacter liquefaciens NBRC 12388T. Further, 121 strains of Gluconobacter were divided into 13 sequence groups (GB1-GB13). Of these, GB1 and GB6 seemed to constitute 2 distinct lineages in the genus. Based on these results, 155 Thai strains were deposited from the BIOTEC culture collection (BCC) in the NITE Biological Resource Center (NBRC) for public use.
    [Show full text]