Is Honey the Same As Sugar?
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Utilization of Cellulose Oligosaccharides by Cellvibrio Gilvus MARION L
JOURNAL OF BACTERIOLOGY, Jan., 1965 Vol. 89, No. 1 Copyright © 1965 American Society for Microbiology Printed in U.S.A. Utilization of Cellulose Oligosaccharides by Cellvibrio gilvus MARION L. SCHAFER' AND KENDALL W. KING Department of Biochemistry and Nutrition, Virginia Polytechnic Institute, Blacksburg, Virginia Received for publication 19 August 1964 ABSTRACT SCHAFER, MARION L. (Virginia Polytechnic Institute, Blacksburg), AND KENDALL W. KING. Utilization of cellulose oligosaccharides by Cellvibrio gilvus. J. Bacteriol. 89: 113-116. 1965.-The hypothesis that oligosaccharides of the cellulose polymer series can be absorbed by cellulolytic bacteria, prior to hydrolysis to the level of glucose or cello- biose, has been tested. Resting-cell suspensions of Cellvibrio gilvus removed oligosac- charides of one to six monomer units from solution at a rate providing the cells with 37 X 106 to 42 X 106 molecules of glucose per cell per minute. There was no concurrent extracellular hydrolysis of the oligosaccharides. The fact that the rate of up- take was constant indicates that an active absorption system is involved. Filtrates from washed-cell suspensions before or after exposure to the oligosaccharides were in- capable of hydrolyzing the sugars. In media where the carbohydrate concentration was growth-limiting, the larger members of the oligosaccharide series supported greater final cell densities than the smaller sugars, but there were no recognizable differences in the growth rates during the logarithmic-growth phase. Recent reviews of microbial utilization of phosphorylase (Ayers, 1958; Sih and McBee, cellulose as an energy and carbon source have 1955; Hulcher and King, 1958a, b). considered primarily the extracellular events In the present report, the ability of C. -
Carbohydrates Carbohydrates Are One of the Main Macronutrients
Carbohydrates Carbohydrates are one of the main macronutrients. They provide an essential source of energy. They are mainly found in plants, where they are manufactured by photosynthesis. Photosynthesis Photosynthesis is the process by which green plants use sunlight to make sugar (glucose) from carbon dioxide and water. How photosynthesis occurs Plant roots absorb water (H2O) from the soil. Leaves take in carbon dioxide (CO2) from the air. Chlorophyll (green pigment) in leaves absorbs energy from the sun. Result Glucose (sugar) (C6H12O6) is formed. Oxygen (O2) is released into the air. Equation for photosynthesis light energy 6CO2 + 6H2O — C6H12O6 + 6O2 chlorophyll Carbon + dioxide water — glucose + oxygen Elemental composition of carbohydrates Carbohydrates are made up of three elements: carbon (C), hydrogen (H) and oxygen (O). Monosaccharides Structure Chemical formula Examples Sources A simple sugar that C6H12O6 Glucose Fruit contains one simple Fructose Fruit and honey sugar unit. It is the Galactose Digested milk smallest unit of a carbohydrate. Chemical structure Disaccharides Structure Chemical formula Examples Sources Formed when two C12H22O11 Maltose Barley monosaccharides (glucose + glucose) Table sugar join, resulting in the Sucrose Milk loss of a water (H2O) (glucose + fructose) molecule Lactose (condensation (glucose + galactose) reaction). Chemical structure Polysaccharides Structure Chemical formula Examples Sources Formed when three (C6H10O5)n Starch Cereals and potatoes or more (n refers to the Pectin Fruit monosaccharides join number of Glycogen Meat together, resulting in monosaccharides Gums Plants and seaweed the loss of a water joined together) Cellulose Skins of fruit and (H2O) molecule with (dietary fibre) vegetables each new link Nuts (condensation reaction). Chains can be straight or branched. -
Metabolism of Monosaccharides and Disaccharides Glucose Is the Most Common Monosaccharide Consumed by Humans
Metabolism of Monosaccharides and disaccharides Glucose is the most common monosaccharide consumed by humans. Two other monosaccharides that occur in significant amounts in the diet are fructose and galactose. Galactose is an important component of cell structural carbohydrates. Catabolism of fructose and galactose are essential pathways of energy metabolism in the body (both illustrated with blue in the adjacent diagram). About 15-20% of calories in the diet are supplied by fructose (55 g/day). The major source of fructose is the disaccharide sucrose. Entry of fructose is not dependent on insulin. Galactose is an important component Of cell structural carbohydrates. Fructose needs to be phosphorylated to enter the pathway either by hexokinase or fructokinase. Hexokinase has low affinity towards fructose (high Km) therefore unless high concentrations of fructose exist very little fructose will be converted to Fructose 6-P. Fructokinase provides the main mechanism of phosphorylation to fructose 1-P, Fructose 1-P does not convert to Fructose 1,6 bisphosphate but is metabolized to Glyceraldehyde and DHAP by aldolase B. DHAP can enter glycolysis or gluconeogenesis while Glyceraldehyde can be metabolized by a number of pathways. The rate of fructose metabolism is more rapid than that of glucose because trioses formed from fructose 1-phosphate bypass PFK1, the rate limiting step in glycolisis. What disorders are associated with fructose metabolism? Where? First lets summarize the various routes of Fructose metabolism in the diagram. Disorders of fructose metabolism can result from excessive fructose consumption. An increase in fructose 1-P due to rapid phosphorylation. This accumulation leads to sequestering of phosphate (A & B). -
Relationships Among Impurity Components, Sucrose, and Sugarbeet Processing Quality
2 Journal of Sugar Beet Research Vol. 52 Nos. 1 & 2 Relationships Among Impurity Components, Sucrose, and Sugarbeet Processing Quality L. G. Campbell and K.K. Fugate USDA-ARS Northern Crop Science Laboratory, Fargo, ND 58102-2765 Corresponding author: Larry Campbell ([email protected]) DOI: 10.5274/jsbr.52.1.2 ABSTRACT Sodium, potassium, amino-nitrogen, and invert sugar are nat- urally-occurring constituents of the sugarbeet root, referred to as impurities, which impede sucrose extraction during rou- tine factory operations. Three germplasm lines selected for low sodium, potassium, or amino-nitrogen and a line selected for high amino-nitrogen concentration from the same parental population and two lines selected from another source, one for high and the other for low amino-nitrogen concentration, were the basis for examining relationships among the impurity components and between the impurity components and sucrose concentration, sucrose loss to mo- lasses, and sucrose extraction rate. Concentrations of the three impurity components were altered through selection; however, in no case did this result in a consistent significant increase in sucrose concentration or estimates of the propor- tion of the sucrose that would be extracted. Correlation analyses indicated a larger role for sodium than for potas- sium or amino-nitrogen in determining relative sucrose con- centration. Selection for low sodium concentration, however, did not increase the percent extractable sucrose, relative to the parental population. The probability of significant im- provement in the processing quality of elite germplasm by re- ducing the concentration of individual impurity components appears to be low, based upon the populations examined in this study. -
Field Guide to Common Macrofungi in Eastern Forests and Their Ecosystem Functions
United States Department of Field Guide to Agriculture Common Macrofungi Forest Service in Eastern Forests Northern Research Station and Their Ecosystem General Technical Report NRS-79 Functions Michael E. Ostry Neil A. Anderson Joseph G. O’Brien Cover Photos Front: Morel, Morchella esculenta. Photo by Neil A. Anderson, University of Minnesota. Back: Bear’s Head Tooth, Hericium coralloides. Photo by Michael E. Ostry, U.S. Forest Service. The Authors MICHAEL E. OSTRY, research plant pathologist, U.S. Forest Service, Northern Research Station, St. Paul, MN NEIL A. ANDERSON, professor emeritus, University of Minnesota, Department of Plant Pathology, St. Paul, MN JOSEPH G. O’BRIEN, plant pathologist, U.S. Forest Service, Forest Health Protection, St. Paul, MN Manuscript received for publication 23 April 2010 Published by: For additional copies: U.S. FOREST SERVICE U.S. Forest Service 11 CAMPUS BLVD SUITE 200 Publications Distribution NEWTOWN SQUARE PA 19073 359 Main Road Delaware, OH 43015-8640 April 2011 Fax: (740)368-0152 Visit our homepage at: http://www.nrs.fs.fed.us/ CONTENTS Introduction: About this Guide 1 Mushroom Basics 2 Aspen-Birch Ecosystem Mycorrhizal On the ground associated with tree roots Fly Agaric Amanita muscaria 8 Destroying Angel Amanita virosa, A. verna, A. bisporigera 9 The Omnipresent Laccaria Laccaria bicolor 10 Aspen Bolete Leccinum aurantiacum, L. insigne 11 Birch Bolete Leccinum scabrum 12 Saprophytic Litter and Wood Decay On wood Oyster Mushroom Pleurotus populinus (P. ostreatus) 13 Artist’s Conk Ganoderma applanatum -
Florida Blueberry Pollination Factsheet
Florida Blueberry PROJECT ICP Pollination Blueberries Require Pollination Blueberries need to be cross-pollinated with another cultivar of the same species (rabbiteye or southern highbush blueberry) in order to produce fruit. Cross-pollination allows for better fruit set, berry size, and earlier ripening. Most growers bring in managed European honey bee hives or commercial bumble bees for pollination. Several types of wild bees are also effective and abundant pollinators of Florida blueberries. All of these different kinds of bees visit blueberry flowers to collect pollen and nectar to feed their young. Integrated Crop Pollination: combining strategies to improve pollination Having many different species of pollinators can help ensure reliable pollination. Different species of bees tend to visit flowers at different times of the day and are active at different times throughout the bloom season; having a diverse set of bees active in your fields can ensure consistent pollination from the beginning to the end of crop bloom. Honey bee abundance and wild bee diversity are both important contributors to southeastern US blueberry pollination. Cool, rainy, and windy spring weather can lead to poor pollination. When multiple pollinator species are active, more flowers are likely to be visited on poor weather days. Large-bodied bees, including all three types of wild bees that visit Florida blueberry flowers, stay more active under Pollination is essential for blueberry production. On the left, a blueberry cluster that was enclosed in a mesh bag during cool and cloudy conditions than do honey bees and can help bloom to exclude pollinators. On the right, a blueberry cluster pollinate the crop in variable spring weather. -
FOOD ANALYSIS: Carbohydrate Analysis
FOOD ANALYSIS: Carbohydrate Analysis B. Pam Ismail [email protected] FScN 146 612 625 0147 FOOD ANALYSIS: Carbohydrate Analysis The following is/are a carbohydrate(s): A. Pectin B. Cellulose C. Lignin D. A & B E. B & C F. A & C G. All of the above H. None of the above 1 FOOD ANALYSIS: Carbohydrate Analysis The following is/are a carbohydrate(s): A. Pectin B. Cellulose C. Lignin D. A & B E. B & C F. A & C G. All of the above H. None of the above FOOD ANALYSIS: Carbohydrate Analysis The method used to determine starch gelatinization could be used to determine starch retrogradation A. True B. False 2 FOOD ANALYSIS: Carbohydrate Analysis Importance of Carbohydrates Importance of Analyzing Carbohydrates FOOD ANALYSIS: Carbohydrate Analysis Carbohydrate Classification CH2OH CH2OH HO O O OH OH OH Monosaccharides O Di and oligosaccharides (2-10 units) OH OH Polysaccharides CH2OH HOH C O O 2 OH o Starch HO O HO CH2OH o Dietary fiber OH OH CH2OH CH2OH O O OH OH O O O OH OH CH2OH CH2OH CH2OH CH2 CH2OH CH2OH O O O O OH OH O O OH OH OH OH O O O O O O O OH OH OH OH OH OH 3 FOOD ANALYSIS: Carbohydrate Analysis General Sample Preparation Drying o Vacuum oven Fat extraction o Soxhlet FOOD ANALYSIS: Carbohydrate Analysis Total Carbohydrate Analysis H (research purposes) HO O Phenol-sulfuric acid method O What happens to glycosidic linkages under acidic conditions? strongly acidic conditions furans monosaccharides (heat) CH2OH CH2OH O O enolizations, OH OH OH dehydrating reactions H O HO O OH OH O 4 FOOD ANALYSIS: Carbohydrate Analysis Total Carbohydrate -
Dietary Glycemic Index and Load and the Risk of Type 2 Diabetes: Assessment of Causal Relations
nutrients Review Dietary Glycemic Index and Load and the Risk of Type 2 Diabetes: Assessment of Causal Relations Geoffrey Livesey 1,* , Richard Taylor 1, Helen F. Livesey 1, Anette E. Buyken 2, David J. A. Jenkins 3,4,5,6, Livia S. A. Augustin 4,7, John L. Sievenpiper 3,4,5,6, Alan W. Barclay 8, Simin Liu 9, Thomas M. S. Wolever 3,4, Walter C. Willett 10, Furio Brighenti 11 , Jordi Salas-Salvadó 12,13 , Inger Björck 14, Salwa W. Rizkalla 15, Gabriele Riccardi 16, Carlo La Vecchia 17 , Antonio Ceriello 18, Antonia Trichopoulou 19, Andrea Poli 20, Arne Astrup 21 , Cyril W. C. Kendall 3,4,22, Marie-Ann Ha 23 , Sara Baer-Sinnott 24 and Jennie C. Brand-Miller 25 1 Independent Nutrition Logic Ltd, 21 Bellrope Lane, Wymondham NR180QX, UK; [email protected] (R.T.); [email protected] (H.F.L.) 2 Institute of Nutrition, Consumption and Health, Faculty of Natural Sciences, Paderborn University, 33098 Paderborn, Germany; [email protected] 3 Departments of Nutritional Science and Medicine, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada; [email protected] (D.J.A.J.); [email protected] (J.L.S.); [email protected] (T.M.S.W.); [email protected] (C.W.C.K.) 4 Clinical Nutrition and Risk Factor Modification Centre, St. Michael’s Hospital, Toronto, ON M5C 2T2, Canada; [email protected] 5 Division of Endocrinology and Metabolism, Department of Medicine, St. Michael’s Hospital, Toronto, ON M5C 2T2, Canada 6 Li Ka Shing Knowledge Institute, St. -
FOOD ALLERGEN LABELING All Food Labels Printed After Jan
FOOD ALLERGEN LABELING All food labels printed after Jan. 1, 2006 Must comply with the Food Allergen Labeling and Consumer Protection Act of 2004 (FALCPA) • Eight foods or food groups have been identified as causing 90% of all documented food allergies & represent the foods most likely to result in severe or life-threatening reactions. • These 8 are: peanuts, tree nuts (e.g., walnuts, hazel nuts, almonds), soybeans, wheat, eggs, milk, fish (e.g., bass, flounder, cod), & crustacean shellfish (e.g., crab, lobster, shrimp). Tree nuts, fish, and crustacean shellfish must be listed by individual name, not as group term such as “tree nuts”. • FALCPA requires that food manufacturers label food products that contain an ingredient that is one of the eight major allergens or that contains protein from a major food allergen in one of the following ways: 1. The ingredient statement lists by name all of the allergenic ingredients in the food, for instance: INGREDIENTS: WHOLE WHEAT FLOUR, WATER, PARTIALLY HYDROGENATED SOYBEAN OIL, MILK, EGGS, YEAST, SALT. or 2. The name of the food source is listed in parenthesis following the common or usual name of the major food allergen in the list of ingredients when the name of the food source of the major allergen isn’t a required part of the ingredient name. INGREDIENTS: ENRICHED FLOUR (WHEAT FLOUR, MALTED BARLEY, NIACIN, REDUCED IRON, THIAMIN MONONITRATE, RIBOFLAVIN, FOLIC ACID), SUGAR, PARTIALLY HYDROGENATED SOYBEAN OIL, AND/OR COTTONSEED OIL, HIGH FRUCTOSE CORN SYRUP, WHEY (MILK), ALBUMIN (EGGS), VANILLA, NATURAL AND ARTIFICIAL FLAVORING), SALT, LEAVENING (SODIUM ACID PYROPHOSPHATE, MONOCALCIUM PHOSPHATE), SOY LECITHIN, MONO-AND DIGLYCERIDES (EMULSIFIER). -
Is There Hidden Sugar in Your Drink?
Is There Hidden Sugar in Your Drink? Anjali Shankar 9th Grade Moravian Academy Upper School June 5th, 2020 Motivation - I have a big passion for the medical field, showed by last year’s project. - Food labels and nutrition have caught my eye and are important when eating. How do glucose levels Research in different drinks change after adding Question an invertase enzyme? Given that the invertase enzyme breaks down sucrose, glucose levels will rise after adding the enzyme because the sucrose will convert to Hypothesis glucose and fructose. Coca Cola will have the most glucose because it has the most calories of each drink. Glucose - Chemical compound in the body - C6H12O6 - Comes from food and drink - Generally rich in sugars/carbohydrates - Used for many purposes: - Used to make energy (ATP) in cellular respiration - Stores energy - Used to build carbohydrates Chemical Reaction - A chemical reaction transfers a set of compounds into another - Reactants: Enter into a chemical reaction - Products: Compounds produced by the reaction - Catalyst: Speeds up the rate of a chemical reaction - Enzyme: Biological catalysts; usually proteins The formula for this experiment is: Invertase Sucrose + Water Glucose + Fructose Invertase C12H22O11 + H20 C6H12O6 + C6H12O6 In the Body - The most common sugar is eaten as sucrose. - Also known as table sugar - It is broken down in the body into glucose and fructose through a chemical reaction during digestion. - Fructose: Contains the same elements as glucose, but has a different chemical construction - Often used to make more glucose - The reaction is catalyzed by an enzyme named sucrase. - Modeled by invertase in experiment - The pancreas monitors blood sugar, or amount of glucose in the body. -
Beta-Glucan Effects on Pasting Properties and Potential Health Benefits of Flours from Different Oat Lines Yanjun Liu Iowa State University
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2010 Beta-glucan effects on pasting properties and potential health benefits of flours from different oat lines Yanjun Liu Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Nutrition Commons Recommended Citation Liu, Yanjun, "Beta-glucan effects on pasting properties and potential health benefits of flours from different oat lines" (2010). Graduate Theses and Dissertations. 11303. https://lib.dr.iastate.edu/etd/11303 This Thesis is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Beta-glucan effects on pasting properties and potential health benefits of flours from different oat lines by Yanjun Liu A thesis submitted to the graduate faculty in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Major: Food Science and Technology Program of Study Committee: Pamela J. White, Major Professor Terri Boylston Theodore B. Bailey Iowa State University Ames, Iowa 2010 Copyright © Yanjun Liu, 2010. All rights reserved. ii TABLE OF CONTENTS CHAPTER 1 GENERAL INTRODUCTION 1 Introduction 1 Literature Review 3 Origin of Oats 3 Oat Grain 3 Health Benefits of Oats 6 Oat Milling and Processing -
GRAINS of TRUTH for DIABETES by Jeanne Brown, RD,CDE, LD
GRAINS OF TRUTH FOR DIABETES By Jeanne Brown, RD,CDE, LD Avoiding pasta to lower your blood sugar? Wondering how to identify whole grains? Does the glycemic index (GI) of grains matter? Here are some grains of truth for diabetes. Whole grains provide fiber, vitamins, minerals, antioxidants and in general have a lower GI which reduces the risk of diabetes. Researchers have found a 30 % lower risk of diabetes with high fiber diets from grains. People who eat at least 2 servings per week of brown rice have a 11% lower risk of Type 2 Diabetes than those that eat less. The risk of getting Type 2 Diabetes was 2 ½ times greater for people who had the highest glycemic load and lowest fiber intake in the study. The GI measures how a carbohydrate- containing food raises blood sugar. The GI ranges from 0 to 100. The higher the number, the more likely it will raise blood sugars. The GI is also affected by how the food is processed, how it is cooked and the ripeness of fruit. Pasta cooked just until it is firm and chewy has a lower GI than overcooked pasta. A ripe banana will spike blood sugar more than an unripe banana. Tips: Look for the word “WHOLE” grain. “Whole” ensures they used the entire grain which includes the fiberous bran, and nutrient dense germ. “Wheat bread” or “wheat flour” is not necessarily whole grain unless it states “whole wheat" in the beginning of the ingredient list. Products such as: 100% whole wheat bread, shredded wheat cereal and Triscuit crackers are 100 % whole grains.