Identifying the Misidentification Problems

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Identifying the Misidentification Problems

Henry Malnick, November 2012 The Pitfalls of Identification Chadwick, P.R ., et al. (1995) Haemophilus paraphrophilus infection: a pitfall in laboratory diagnosis. J. Infect (1995) 30: 67-69. Gram-negative catalase negative oxidase positive bacteria Cardiobacterium hominis Eikenella corrodens Kingella denitrificans Kingella kingae Suttonella (Kingella) indologenes Neisseria elongata ss nitroreductans Aggregatibacter aphrophilus (Haemophilus aphrophilus/paraphrophilus) ONPG Lysine Ornithine NO3 NO2 Gas from indole β-haem red. red. NO2 red. E. corrodens + + + - - - - - N. elongate ss - - - + + - - - nitroreductans Kingella - - - + + + - - denitrificans Kingella kingae - - - - - - - + Cardiobacterium - - - - - - + - spp. * Suttonella - - - - - - + - indologenes* Aggregatibacter V V V + - - V V spp. *Cardiobacterium produce acid from sorbitol/mannitol, Suttonella are negative Actinobacillus sp. Aggregatibacter aphrophilus Haemophilus influenzae Haemophilus parainfluenzae Haemophilus sp. Kingella sp. Moraxella sp. Pasteurella multocida, Pasteurella sp. Kingella kingae Misleading clues . Colony morphology . Gram stain . Cell morphology . Atypical biochemical test results Gram-positive bacteria submitted as gram-negative bacteria Arcanobacterium haemolyticum Aerobic spore bearers (Brevibacillus, Paenibacillus) Clostridium tertium Leifsonia (Corynebacterium) aquaticum Exiguobacterium acetylicum Microbacterium spp. Propionibacterium acnes Streptococcus spp. Capnocytophaga Leptotrichia spp. Vancomycin Gram-positive resistant rods Lactobacillus, Erysipelothrix Gram-negative sensitive rods/coccobacilli Moraxella, Acinetobacter Aerobic spore bearers identifying as Sphingomonas paucimobilis A Case of Mistaken Identity Burkholderia pseudomallei Pseudomonas aeruginosa Pseudomonas fluorescens Burkholderia cepacia complex Chromobacterium violaceum Containment level 2 organisms identifying as containment level 3 Morganella morganii and Aggregatibacter actinomycetemcomitans identifies as Burkholderia mallei Chryserobacterium spp. identifies as Francisella tularensis Acknowledgement for the Lactobacillus photographs Shila Seaton, Elizabeth Fagan (QAL) Acknowledgement for the Bacillus photographs Ijeoma Nwafor, Obioma Mpamugo, Nafeesa Hussain (Bacillus Reference Unit, HPA Colindale) .

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BD-CS-057, REV 0 | AUGUST 2017 | Page 1
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