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The Cell Cycle Abstracts of papers presented at the 2010 meeting on THE CELL CYCLE May 18–May 22, 2010 CORE Metadata, citation and similar papers at core.ac.uk Provided by Cold Spring Harbor Laboratory Institutional Repository Cold Spring Harbor Laboratory Cold Spring Harbor, New York Abstracts of papers presented at the 2010 meeting on THE CELL CYCLE May 18–May 22, 2010 Arranged by Sue Biggins, Fred Hutchinson Cancer Research Center Nicholas Dyson, Massachusetts General Hospital Cancer Center Johannes Walter, Harvard Medical School Cold Spring Harbor Laboratory Cold Spring Harbor, New York This meeting was funded in part by the National Cancer Institute, a branch of the National Institutes of Health. Contributions from the following companies provide core support for the Cold Spring Harbor meetings program. Corporate Sponsors Agilent Technologies AstraZeneca BioVentures, Inc. Bristol-Myers Squibb Company Genentech, Inc. GlaxoSmithKline Hoffmann-La Roche Inc. Life Technologies (Invitrogen & Applied Biosystems) Merck (Schering-Plough) Research Laboratories New England BioLabs, Inc. OSI Pharmaceuticals, Inc. Sanofi-Aventis Plant Corporate Associates Monsanto Company Pioneer Hi-Bred International, Inc. Foundations Hudson-Alpha Institute for Biotechnology _________________________________________________________ Cover: Artwork by Sergiy I. Borysov, University of South Florida. THE CELL CYCLE Tuesday, May 18 – Saturday, May 22, 2010 Tuesday 7:30 pm Keynote Speaker 1 Chromosome Cohesin and Condensation Wednesday 9:00 am 2 G1 and Cdk Regulation Wednesday 2:00 pm 3 Poster Session I Wednesday 4:30 pm Wine and Cheese Party * Wednesday 7:30 pm 4 S-Phase Thursday 9:00 am 5 The DNA Damage Response Thursday 2:00 pm 6 Poster Session II Thursday 7:30 pm 7 Cell Cycle Regulatory Pathways Friday 9:00 am 8 Chromosome Segregation Friday 2:00 pm 9 Metaphase / Anaphase Friday 6:00 pm Concert 7:00 pm Banquet Saturday 9:00 am 10 Proteolysis Poster sessions are located in Bush Lecture Hall * Airslie Lawn, weather permitting Mealtimes at Blackford Hall are as follows: Breakfast 7:30 am-9:00 am Lunch 11:30 am-1:30 pm Dinner 5:30 pm-7:00 pm Bar is open from 5:00 pm until late Abstracts are the responsibility of the author(s) and publication of an abstract does not imply endorsement by Cold Spring Harbor Laboratory of the studies reported in the abstract. These abstracts should not be cited in bibliographies. Material herein should be treated as personal communications and should be cited as such only with the consent of the author. Please note that recording of oral sessions by audio, video or still photography is strictly prohibited except with the advance permission of the author(s), the organizers, and Cold Spring Harbor Laboratory. Printed on 100% recycled paper. PROGRAM TUESDAY, May 18—7:30 PM SESSION 1 CHROMOSOME COHESIN AND CONDENSATION Chairperson: D. Morgan, University of California, San Francisco J.-M. Peters, Research Institute of Molecular Pathology, Vienna, Austria KEYNOTE SPEAKER Douglas Koshland University of California, Berkeley “Lessons learned from trying to answer to a higher order" Cohesin and sororin are recruited to chromatin by distinctly different mechanisms Jane Song, Andrea L. Lafont, Susannah Rankin. Presenter affiliation: Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma. 1 Identification and characterization of a novel cohesin proteinase mediating sister chromatid segregation Anil K. Panigrahi, Debananda Pati. Presenter affiliation: Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas. 2 Cohesin subunit Scc1 promotes caspase activation and cell death in mitosis Laura A. Diaz-Martinez, Zemfira Karamysheva, Soonjoung Kim, Hongtao Yu. Presenter affiliation: Howard Hughes Medical Institute and UT Southwestern Medical Center, Dallas, Texas. 3 Condensin and microtubule tension drive de-catenation by topoisomerase II through stable modification of DNA topology Jonathan Baxter, Jorge B. Schvartzman, John F. Diffley, Luis Aragon. Presenter affiliation: MRC Clincial Sciences Centre, London, United Kingdom. 4 v Regulation of sister chromatid cohesion and chromatin structure by cohesin Jan-Michael Peters. Presenter affiliation: Research Institute of Molecular Pathology, Vienna, Austria. 5 WEDNESDAY, May 19—9:00 AM SESSION 2 G1 AND CDK REGULATION Chairperson: S. Haase, Duke University, Durham, North Carolina J. Lees, Massachusetts Institute of Technology, Cambridge Cycling without cyclins—CDKs are modulators and effectors of a transcription network oscillator Steven B. Haase, Laura A. Simmons Kovacs, David A. Orlando, Michael B. Mayhew, Sayan Mukherjee. Presenter affiliation: Duke University, Durham, North Carolina. 6 Cell cycle commitment Andreas Doncic, Jan M. Skotheim. Presenter affiliation: Stanford University, Stanford, California. 7 A sequence of counteracting phosphorylations restricts an asymmetric gene expression program to early G1 Emily Mazanka, Eric L. Weiss. Presenter affiliation: Northwestern University, Evanston, Illinois. 8 Different levels of cyclin-Cdk order mitotic events in S. cerevisiae Catherine Oikonomou, Frederick Cross. Presenter affiliation: Rockefeller University, New York, New York. 9 Bistability may allow for wave-like propagation of mitotic activity and maintain cell synchronization Jeremy B. Chang, James E. Ferrell Jr. Presenter affiliation: Stanford University, Stanford, California. 10 vi The DNA replication checkpoint promotes E2F-dependent cell- cycle transcription in response to a DNA replication block Cosetta Bertoli, Tatyana I. Kalashnikova, Clare H. McGowan, Curt Wittenberg, Robertus A. de Bruin. Presenter affiliation: MRC Laboratory for Molecular Cell Biology, UCL, London, United Kingdom. 11 The MuvB complex differentially regulates the expression of early and late cell cycle genes Subhashini Sadasivam, Yong Zhang, Velmurugan Soundarapandian, X. Shirley Liu, James A. DeCaprio. Presenter affiliation: Dana-Farber Cancer Institute, Boston, Massachusetts. 12 E2F regulation is dispensable for growth control mediated by pRB Matthew J. Cecchini, Frederick A. Dick. Presenter affiliation: London Regional Cancer Program, Children’s Health Research Institute, London, Canada. 13 Dissecting the unique role of the retinoblastoma tumor suppressor during cellular senescence Agustin Chicas, Xiaowo Wang, Chaolin Zhang, Zhen Zhao, Mila McCurrach, Scott W. Lowe. Presenter affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 14 The retinoblastoma protein regulates fate choice and lineage commitment Eliezer Calo, Jacqueline Lees. Presenter affiliation: David H. Koch Institute of Integrative Cancer Research, MIT, Cambridge, Massachusetts. 15 WEDNESDAY, May 19—2:00 PM SESSION 3 POSTER SESSION I Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase Bungo Akiyoshi, Sue Biggins. Presenter affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington. 16 vii Max1 links MBF-dependent transcription to completion of S phase Gómez-Escoda Blanca, Ivanova Tsvetomira, Calvo A. Isabel, Alves- Rodrigues Isabel, Hidalgo Elena, Ayté José. Presenter affiliation: Universitat Pompeu Fabra, Barcelona, Spain. 17 Role of ATR and CHK1 haploinsufficiency in replication and chromosomal fragility Hayat Arzouk, Zeina Majed, Sandie Tuduri, Helene Fontaine, Charles Theillet, Arnaud Coquelle. Presenter affiliation: Cancer Research Institute of Monptellier (IRCM), Montpellier, France. 18 GCP6, a component of the gamma-TuRC is required for centrosome duplication Ramona Bahtz, Marc Arnold, Ingrid Hoffmann. Presenter affiliation: German Cancer Research Center, Heidelberg, Germany. 19 The molecular chaperone Hsp90 is required for cell cycle exit in Drosophila Jennifer L. Bandura, Huaqi Jiang, Bruce A. Edgar. Presenter affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington. 20 Protein-protein interactions link transcription factor network to Clb regulation in the yeast cell cycle Matteo Barberis, Christian Linke, Hans Lehrach, Edda Klipp, Sylvia Krobitsch. Presenter affiliation: Humboldt University Berlin, Berlin, Germany; Max Planck Institute for Molecular Genetics, Berlin, Germany. 21 Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins in budding yeast Matteo Barberis, Christian Linke, Miquel A. Adrover, Hans Lehrach, Francesc Posas, Sylvia Krobitsch, Edda Klipp. Presenter affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany; Humboldt University Berlin, Berlin, Germany. 22 The Skp2 promoter integrates signaling through the NF-κB/IKK, p53 and Akt/GSK3β pathways to regulate autophagy and apoptosis in response to DNA damage Benjamin Barré, Neil D. Perkins. Presenter affiliation: Centre Paul Papin, Angers, France. 23 viii Regulation of Aurora B kinase by Cullin3 mediated ubiquitination Jochen Beck, Sarah Maerki, Markus Posch, Jason R. Swedlow, Izabela Sumara, Matthias Peter. Presenter affiliation: ETH Zurich, Zurich, Switzerland. 24 Tyrosine phosphorylation of p27Kip1 serves as a cyclin D-cdk4 ON/OFF switch Melissa James, Nuria Ferrandiz, Weizhen Ye, Stacy W. Blain. Presenter affiliation: SUNY Downstate Medical Center, Brooklyn, New York. 25 Regulation of S. cerevisiae Glc7 (PP1) cell cycle function by Shp1 Stefanie Böhm, Alexander Buchberger. Presenter affiliation: University of Würzburg, Würzburg, Germany. 26 Defects in cell cycle checkpoint functions are specific to breast cancer molecular subtypes Jacquelyn J. Bower, Leah D. Vance, William K. Kaufmann. Presenter affiliation: Lineberger Comprehensive Cancer Center, Chapel
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