Evaluation of Saflufenacil on Glyphosate-Resistant And
Total Page:16
File Type:pdf, Size:1020Kb
ABSTRACT EVALUATION OF SAFLUFENACIL ON GLYPHOSATE- RESISTANT AND GLYPHOSATE-PARAQUAT RESISTANT HAIRY FLEABANE (Conyza bonariensis) Hairy fleabane [Conyza bonariensis (L.) Cronq.] is a problematic weed in crop and non-crop areas of California. This problem has been further aggravated by the discovery of herbicide-resistant biotypes. Three experiments were conducted to determine the efficacy of saflufenacil (TreevixTM), a fairly new herbicide, on glyphosate-susceptible (GS), glyphosate-resistant (GR), and glyphosate-paraquat-resistant (GPR) hairy fleabane plants. The studies evaluated the efficacy of saflufenacil when applied alone or in combination with glyphosate at: a) three growth stages (5- to 8-leaf seedling, rosette, and bolting); b) three temperature regimes (15/10ºC, 25/20ºC, 35/30ºC at day/night); and c) three light regimes (100%, 50%, 30%, 0% of full sun). Results differed between experiments conducted in the spring and fall. Saflufenacil-alone was more effective in the fall than in spring. All the GS, GR and GPR plants were controlled by saflufenacil- alone at the 5-to 8-leaf seedling and rosette stage but level of control declined at the bolting stage. Better control was obtained at the 15/10ºC and 25/20ºC than at the 35/30ºC temperature regime. Light regime had no effect on the efficacy of saflufenacil. Efficacy of saflufenacil-alone was inconsistent in spring and varied between the biotypes. Therefore, saflufenacil-alone can provide excellent control of hairy fleabane plants prior to the bolting stage in the fall; but in spring, it will be more effective when applied with glyphosate. Michelle Dennis May 2015 EVALUATION OF SAFLUFENACIL ON GLYPHOSATE- RESISTANT AND GLYPHOSATE-PARAQUAT RESISTANT HAIRY FLEABANE (Conyza bonariensis) by Michelle Dennis A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Plant Science in the Jordan College of Agricultural Sciences and Technology California State University, Fresno May 2015 © 2015 Michelle Dennis APPROVED For the Department of Plant Science: We, the undersigned, certify that the thesis of the following student meets the required standards of scholarship, format, and style of the university and the student's graduate degree program for the awarding of the master's degree. Michelle Dennis Thesis Author Anil Shrestha (Chair) Plant Science John Bushoven Plant Science Kurt Hembree University of California, Cooperative Extension For the University Graduate Committee: Dean, Division of Graduate Studies AUTHORIZATION FOR REPRODUCTION OF MASTER’S THESIS X I grant permission for the reproduction of this thesis in part or in its entirety without further authorization from me, on the condition that the person or agency requesting reproduction absorbs the cost and provides proper acknowledgment of authorship. Permission to reproduce this thesis in part or in its entirety must be obtained from me. Signature of thesis author: ACKNOWLEDGMENTS I would like to dedicate this thesis to my two children, Rio Renee and Ty “Ox” Dennis. If fate ever leads you to read this when you are grown, realize that it is not the printed words providing clarity to well thought-out studies that created this document. It is all of the mistakes that were made, the trials that were thrown out, and the pages of gibberish that needed to be rewritten that brought me to the completion of this document and the true discoveries. To my husband, Scott, thank you for supporting my journey. For the first time in our 15 years of marriage, I truly can’t find the words. To my parents, thank you for having faith in me every step of the way. To my committee, especially my advisor, Anil Shrestha, thank you for not accepting anything less than 100%. Your insights, questions and critiques during this process have given me more confidence in my own abilities going forward. The world of research was foreign to me and now I have a roadmap to follow. To my fellow graduate student, Sonia Rios, I couldn’t have finished this project without the help and constant motivation that you provided to everyone in the weed science program. I wish you nothing but success in your future. I would also like to thank all of the students, faculty and staff of the Jordan College of Agricultural Sciences and Technology at California State University, Fresno that contributed to this study and the California State University Agricultural Research Institute for funding this project. TABLE OF CONTENTS Page LIST OF TABLES ................................................................................................. vii INTRODUCTION .................................................................................................... 1 LITERATURE REVIEW ......................................................................................... 2 Hairy Fleabane Biology and Dispersal ............................................................. 2 Evolution of Herbicide Resistant Hairy Fleabane in the Central Valley .......... 3 Need for Immediate Alternative Herbicides ..................................................... 5 PPO Inhibitors ................................................................................................... 6 Interaction of Herbicides with Plant Physiology and Environmental Factors .................................................................................................... 9 Objectives ........................................................................................................ 11 EXPERIMENT 1 – EFFECT OF GROWTH STAGE OF HAIRY FLEABANE ON THE EFFICACY OF ALTERNATIVE HERBICIDES ............................................................................................. 13 Methods and Materials .................................................................................... 13 Results and Discussion .................................................................................... 16 EXPERIMENT 2 – TEMPERATURE ................................................................... 27 Methods and Materials .................................................................................... 27 Discussion ....................................................................................................... 31 EXPERIMENT 3 – LIGHT INTENSITY .............................................................. 40 Methods and Materials .................................................................................... 40 Results and Discussion .................................................................................... 43 CONCLUSION ...................................................................................................... 51 REFERENCES ....................................................................................................... 54 LIST OF TABLES Page Table 1. Transplanting and herbicide application dates of the different targeted growth stages of glyphosate-susceptible, glyphosate+paraquat resistant, and glyphosate-resistant hairy fleabane plants in 2012 and 2013. ......................................................................................................... 14 Table 2. Mortality of glyphosate-susceptible (GS), glyphosate+paraquat resistant (GPR), glyphosate-resistant (GS), and aboveground biomass (mean of all three biotypes) of hairy fleabane plants treated with herbicides at the 5- to 8-leaf stage in Fall 2012 and Spring 2013. .......... 18 Table 3. Mortality and aboveground biomass of glyphosate-susceptible (GS), glyphosate+paraquat resistant (GPR), glyphosate-resistant (GS) of hairy fleabane plants treated with herbicides at the rosette stage. ........... 21 Table 4. Mortality of glyphosate-susceptible (GS), glyphosate+paraquat resistant (GPR), glyphosate-resistant (GS) in fall 2012 and spring 2013, and aboveground biomass (mean of all three biotypes) in fall 2012 and each biotype in spring 2013 of hairy fleabane plants treated with herbicides at the bolting stage. ......................................................... 23 Table 5. Transplanting and herbicide application dates of glyphosate- susceptible, glyphosate-resistant, and glyphosate+paraquat resistant hairy fleabane plants in 2012 and 2013. .................................................. 28 Table 6. Analysis of variance (ANOVA) table showing the main effects and interactions for plant mortality and aboveground plant biomass of the glyphosate-susceptible, glyphosate-resistant, and glyphosate-paraquat resistant populations of hairy fleabane exposed to three different temperature regimes and treated with various herbicides in 2012 and 2013. ......................................................................................................... 32 Table 7. Plant mortality and aboveground biomass of hairy fleabane plants (averaged for glyphosate-susceptible, glyphosate-resistant, and glyphosate+paraquat resistant biotypes) after treatment and exposure to 15/10˚C temperature. ........................................................................... 34 Table 8. Plant mortality and aboveground biomass of hairy fleabane plants (averaged for glyphosate-susceptible, glyphosate-resistant, and glyphosate+paraquat resistant biotypes) after treatment and exposure to 25/20˚C temperature. ........................................................................... 35 viii viii Page Table 9. Plant mortality of glyphosate-susceptible (GS), glyphosate-resistant (GR), and glyphosate+paraquat resistant (GPR) hairy fleabane plants in 2012 and 2013 (averaged for the three biotypes)