DOCSLIB.ORG

Profiling and Characterization of the Micrornas and Their Targets in Important Vegetables of Solanaceae

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Profiling and Characterization of the Micrornas and Their Targets in Important Vegetables of Solanaceae Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae PhD Thesis (Botany) Submitted By Muhammad Din PhD Scholar Department of Botany University of Balochistan, Quetta Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae PhD Thesis (Botany) Supervised by Dr. Muhammad Younas Khan Barozai Associate Professor Department of Botany University of Balochistan, Quetta Submitted by Muhammad Din PhD Scholar Department of Botany University of Balochistan, Quetta Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae Submitted by Muhammad Din PhD Scholar Department of Botany University of Balochistan, Quetta Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae Thesis submitted for the requirement of the degree of Doctor of Philosophy (PhD) in Botany, University of Balochistan, Quetta Supervised By Dr. Muhammad Younas Khan Barozai Associate Professor Department of Botany, University of Balochistan, Quetta Submitted by Muhammad Din PhD Scholar Department of Botany University of Balochistan, Quetta CERTIFICATE This is to certify that Mr. Muhammad Din who was registered in Doctor of Philosophy (PhD) (Registration No. 1993/UB-2013/R-215) in Botany, Department of Botany, University of Balochistan, Quetta, under the supervision of Dr. Muhammad Younas Khan Barozai, has successfully completed course work of eighteen (18) credit hours and research work under the title “Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae”. He may be allowed to submit the thesis on the above cited topic to the University of Balochistan for the fulfillment of PhD (Botany) degree. Dr. Muhammad Younas Khan Barozai Prof. Dr. Atta Muhammad Sarangzai Research Supervisor Chairperson Associate Professor Department of Botany, Department of Botany, University of Balochistan, Quetta University of Balochistan, Quetta . Dean External Examiner Faculty of Life Sciences University of Balochistan, Quetta I CERTIFICATE It is certified that the said dissertation is based on research work under the title “Profiling and Characterization of the MicroRNAs and Their Targets in Important Vegetables of Solanaceae” and its result carried out by me. This is written and compiled by me. This research work has not been submitted for higher studies in any other institution. MUHAMMAD DIN PhD Scholar Department of Botany, University of Balochistan, Quetta II ACKNOWLEDGEMENT First of all I am thankful to Almighty Allah for giving me such contentment and vision for accomplishment to my research work and compilation of this Thesis. I am sincerely obliged to my supervisor Dr. Muhammad Younas Khan Barozai, Associate Professor, Department of Botany, University of Balochistan, Quetta, for his precious guidance, proper supervision and appreciation during the period of research work. I wish to express my gratitude to Chairperson and all faculty members, Department of Botany, University of Balochistan, Dean, Faculty of Life Sciences, University of Balochistan, Quetta, for their significant guidance, encouragement, helpful suggestions and full cooperation. I would like to thank my humble colleagues for their moral support and helpful suggestions. III DEDICATION This thesis is dedicated to my beloved parents who have supported me all the way since the beginning of my studies and to my supervisor Dr. Muhammad Younas Khan Barozai who has been a great source of motivation and inspiration. Also, this thesis is dedicated to my wife and children (Ashfaq Ahmed and Irshad Ahmed). Finally, this thesis is dedicated to all those who believe in the richness of learning. IV INDEX CONTENT PAGE Certificate I Acknowledgment III Abbreviations X Abstract 2 1. Introduction 4 2. Review of Literature 9 2.1. Solanaceae 9 2.1.1. Tomato (Solanum lycopersicum L.) 9 2.1.2. Potato (Solanum tuberosum L.) 10 2.1.3. Eggplant (Solanum melongena L.) 10 2.1.4. Chilli (Capsicum annuum L.) 11 2.2. MicroRNA (miRNA) 12 2.2.1. History of miRNA 12 2.2.2. Biogenesis of miRNA 13 2.2.3. Function of miRNA 14 2.2.4. Mechanism of miRNA Action 15 2.2.4.1. Cleavage of mRNA directed by miRNA 15 2.2.4.2. Translational repression directed by miRNA 16 2.2.5. Cluster of miRNA 16 2.2.6. Secondary structure of miRNA 17 2.2.7. Convergence and divergence of miRNA in plants 18 2.2.8. Nomenclature of miRNA 19 2.3. Profiling and characterization strategies for miRNA 20 V 2.3.1. Computer-based (bioinformatics) 20 2.3.2. Experimental approach 22 2.4. Computer-based Databases (dbs) and tools 22 2.4.1. Databases (dbs) 22 2.4.1.1. The miRBase 22 2.4.1.2. Plant miRNA database (PMRD) 23 2.4.1.3. GenBank Database (dbGenBank) 24 2.4.1.4. Express Sequence Tags (ESTs)/Database (dbEST) 25 2.4.2. Computer-based tools 25 2.4.2.1. Mfold RNA folding server 25 2.4.2.2. RNAhybrid for targets prediction 26 2.4.2.3. BLAST (Basic Local Alignment Search Tool) 27 2.4.2.4. ClustalW 28 2.4.2.5. WebLogo (A Sequence Logo Generator) 29 3. Materials and Methods 31 3.1. Reference microRNA (miRNA) fetching 31 3.2. ESTs from EST Database (dbEST) 33 3. 3. Profiling initial potential miRNAs 33 3.4. Creation of single tone EST 33 3.5. Removal of protein coding sequences through BLASTx 34 3.6. Generation of stem-loop secondary structures 34 3.7. Physical scrutinization 35 3.8. Reverse Transcription PCR (RT-PCR) validation 35 VI 3.9. Gel visualization 35 3.10. Conservation analysis 38 3.11. Phylogenetic studies 38 3.12. Prediction of miRNA targets 39 3.12.1. A plant small RNA target analysis server (psRNATarget) 39 3.12.2. BLAST and RNAhybrid approach 40 3.13. Gene Ontology (GO) analysis 40 4. Results 42 4.1. Tomato miRNAs 42 4.1.1. Tomato new conserved miRNAs 42 4.1.2. Tomato miRNAs characterization 43 4.1.3. Tomato miRNAs experimental validation by RT-PCR 54 4.1.4. Tomato miRNAs convergence and phylogenetic investigation 54 4.1.5. Tomato potential miRNAs targets 55 4.2. Potato miRNAs 63 4.2.1. Potato new conserved miRNAs 63 4.2.2. Potato miRNAs characterization 68 4.2.3. Potato miRNAs experimental validation by RT-PCR 69 4.2.4. Potato sense/antisense miRNAs 73 4.2.5. Potato cluster miRNAs gene 74 4.2.6. Potato miRNAs convergence and phylogenetic investigation 75 4.2.7. Potato potential miRNAs targets 76 4.3. Eggplant miRNAs 82 VII 4.3.1. Eggplant new conserved miRNAs 82 4.3.2. Eggplant miRNAs characterization 82 4.3.3. Eggplant miRNAs experimental validation by RT-PCR 87 4.3.4. Eggplant miRNAs convergence and phylogenetic investigation 87 4.3.5. Eggplant potential miRNAs targets 87 4.4. Chilli miRNAs 98 4.4.1. Chilli new conserved miRNAs 98 4.2.2. Chilli miRNAs characterization 102 4.4.3. Chilli miRNAs validation through BLASTx 103 4.4.4. Chilli pre-miRNA cluster 108 4.4.5. Chilli miRNAs convergence and phylogenetic investigation 109 4.4.6. Chilli potential miRNAs targets 111 5. Discussion 118 6. Conclusion 133 7. References 135 8. Publications from the dissertation 151 TABLES Table 1. Solanum plant species miRNAs primers for RT-PCR expression analysis 36 Table 2. Tomato miRNAs characterization 44 Table 3. Tomato miRNAs targets 57 Table 4. Potato miRNAs characterization 64 Table 5. Potato miRNAs targets 77 Table 6. Eggplant miRNAs characterization 84 Table 7. Eggplant miRNAs targets 94 Table 8. Chilli miRNAs characterization 99 VIII Table 9. Chilli miRNAs targets 112 FIGURES Figure 1. Flow-chart for the computational identification of tomato, potato, eggplant 32 and chilli putative miRNAs using all known matures and precursors miRNAs in plants as a reference miRNAs Figure 2. Tomato pre-miRNA secondary structures 49 Figure 3. Tomato miRNA RT-PCR expressional validation 54 Figure 4. Tomato miRNA convergence investigation 56 Figure 5. Tomato miRNA phylogenetic investigation 56 Figure 6. Potato pre-miRNA secondary structures 70 Figure 7. Experimental validation of potato miRNAs by RT-PCR 73 Figure 8. Potato sense/antisense miRNA gene 73 Figure 9. Potato pre-miRNA cluster 74 Figure 10. Potato miRNA convergence study 75 Figure 11. Potato miRNA phylogenetic study 76 Figure 12. Eggplant pre-miRNA secondary structures 88 Figure 13. RT-PCR expressional validation for eggplant miRNAs 93 Figure 14. Eggplant miRNAs conservation studies 93 Figure 15. Eggplant miRNAs phylogenetic analysis 93 Figure 16. Chilli pre-miRNA secondary structures 105 Figure 17. Chilli pre-miRNA clusters 108 Figure 18. Chilli miRNA convergence study 110 Figure 19. Chilli miRNA phylogenetic study 110 IX ABBREVIATIONS Abscisic acid (ABA) Minimum Free Energy (MFE) Anthocyanin synthase (ANS) Myeloblastosis (MYB) Arabidopsis lyrata (aly) National Center for Biotechnology Information (NCBI) Arabidopsis thaliana (ath) Nicotiana tabacum (nta) ATP-binding cassette (ABC) Nucleotide-binding site-leucine rich repeat (NBS-LRR) Base pair (bp) Nucleotides (nt) Basic local alignment search tool Number of mismatches (NM) (BLAST) Brachypodium distachyon (bdi) Opposite strand of miRNA (miRNA*) Brassica rapa (bra) Organ of expression (OE) cAMP response element binding (CREB) Orthophosphate (Pi) Capsicum annuum (can) Oryza sativa (osa) Carotenoid cleavage dioxygenase (CCD) Phenyl ammonium lyase (PAL) Citrus sinensis (csi) Plant small RNA target (psRNATarget) Complementary DNA (cDNA) Populus trichocarpa (ptc) Database of EST (dbEST) Precursor miRNA length (PL) Dicer-like Enzyme1 (DCL1) Precursor of miRNAs (pre-miRNAs) Double-stranded RNA (dsRNA) Primary transcripts of mature miRNAs (pri-miRNAs) Expressed Sequence
Load more

Recommended publications
  • The Bone Marrow Niche in the Pathogenesis of Multiple Myeloma a Role for Wnt Signaling and Adrenomedullin
    A role for Wnt signaling and Adrenomedullin and signaling Wnt for A role Myeloma Multiple of niche in the pathogenesis marrow The bone The bone marrow niche in the pathogenesis of Multiple Myeloma A role for Wnt signaling and Adrenomedullin pracownia DTP i grafiki www.przygotowalniaDTP.pl ISBN 978-83-63861-16-2 Kinga Anna Kocemba okladka_nowa.indd 1-3 2014-01-20 11:57:46 The bone marrow niche in the pathogenesis of Multiple Myeloma A role for Wnt signaling and Adrenomedullin The bone marrow niche in the pathogenesis of Multiple Myeloma A role for Wnt signaling and Adrenomedullin Kinga Anna Kocemba The research described in this thesis was funded by the Dutch Cancer Society Editing Przygotowalnia Pracownia DTP i Grafiki Graphic design and typesetting Przygotowalnia Pracownia DTP i Grafiki Cover: Antelope Canyon, Arizona, USA by Kinga Anna Kocemba © Copyright by Kinga Anna Kocemba ISBN 978-83-63861-16-2 Amsterdam–Kraków 2014 Pracownia DTP i Grafiki ul. Łużycka 71c/9 30–693 Kraków [email protected] www.przygotowalniaDTP.pl Moim rodzicom To my parents The bone marrow niche in the pathogenesis of Multiple Myeloma A role for Wnt signaling and Adrenomedullin academisch proefschrift ter verkrijging van de graad van doctor aan de Universiteit van Amsterdam op gezag van de Rector Magnificus prof. dr. D.C. van den Boom ten overstaan van een door het college voor promoties ingestelde commissie, in het openbaar te verdedigen in de Agnietenkapel op donderdag 6 maart 2014, te 14:00 uur door Kinga Anna Kocemba geboren te Oświęcim, Polen Promotiecommissie promotor Prof.
    [Show full text]
  • Public Sector Development Programme 2019-20 (Original)
    GOVERNMENT OF BALOCHISTAN PLANNING & DEVELOPMENT DEPARTMENT PUBLIC SECTOR DEVELOPMENT PROGRAMME 2019-20 (ORIGINAL) Table of Contents S.No. Sector Page No. 1. Agriculture……………………………………………………………………… 2 2. Livestock………………………………………………………………………… 8 3. Forestry………………………………………………………………………….. 11 4. Fisheries…………………………………………………………………………. 13 5. Food……………………………………………………………………………….. 15 6. Population welfare………………………………………………………….. 16 7. Industries………………………………………………………………………... 18 8. Minerals………………………………………………………………………….. 21 9. Manpower………………………………………………………………………. 23 10. Sports……………………………………………………………………………… 25 11. Culture……………………………………………………………………………. 30 12. Tourism…………………………………………………………………………... 33 13. PP&H………………………………………………………………………………. 36 14. Communication………………………………………………………………. 46 15. Water……………………………………………………………………………… 86 16. Information Technology…………………………………………………... 105 17. Education. ………………………………………………………………………. 107 18. Health……………………………………………………………………………... 133 19. Public Health Engineering……………………………………………….. 144 20. Social Welfare…………………………………………………………………. 183 21. Environment…………………………………………………………………… 188 22. Local Government ………………………………………………………….. 189 23. Women Development……………………………………………………… 198 24. Urban Planning and Development……………………………………. 200 25. Power…………………………………………………………………………….. 206 26. Other Schemes………………………………………………………………… 212 27. List of Schemes to be reassessed for Socio-Economic Viability 2-32 PREFACE Agro-pastoral economy of Balochistan, periodically affected by spells of droughts, has shrunk livelihood opportunities.
    [Show full text]
  • Investigation of Candidate Genes and Mechanisms Underlying Obesity
    Prashanth et al. BMC Endocrine Disorders (2021) 21:80 https://doi.org/10.1186/s12902-021-00718-5 RESEARCH ARTICLE Open Access Investigation of candidate genes and mechanisms underlying obesity associated type 2 diabetes mellitus using bioinformatics analysis and screening of small drug molecules G. Prashanth1 , Basavaraj Vastrad2 , Anandkumar Tengli3 , Chanabasayya Vastrad4* and Iranna Kotturshetti5 Abstract Background: Obesity associated type 2 diabetes mellitus is a metabolic disorder ; however, the etiology of obesity associated type 2 diabetes mellitus remains largely unknown. There is an urgent need to further broaden the understanding of the molecular mechanism associated in obesity associated type 2 diabetes mellitus. Methods: To screen the differentially expressed genes (DEGs) that might play essential roles in obesity associated type 2 diabetes mellitus, the publicly available expression profiling by high throughput sequencing data (GSE143319) was downloaded and screened for DEGs. Then, Gene Ontology (GO) and REACTOME pathway enrichment analysis were performed. The protein - protein interaction network, miRNA - target genes regulatory network and TF-target gene regulatory network were constructed and analyzed for identification of hub and target genes. The hub genes were validated by receiver operating characteristic (ROC) curve analysis and RT- PCR analysis. Finally, a molecular docking study was performed on over expressed proteins to predict the target small drug molecules. Results: A total of 820 DEGs were identified between
    [Show full text]
  • Noelia Díaz Blanco
    Effects of environmental factors on the gonadal transcriptome of European sea bass (Dicentrarchus labrax), juvenile growth and sex ratios Noelia Díaz Blanco Ph.D. thesis 2014 Submitted in partial fulfillment of the requirements for the Ph.D. degree from the Universitat Pompeu Fabra (UPF). This work has been carried out at the Group of Biology of Reproduction (GBR), at the Department of Renewable Marine Resources of the Institute of Marine Sciences (ICM-CSIC). Thesis supervisor: Dr. Francesc Piferrer Professor d’Investigació Institut de Ciències del Mar (ICM-CSIC) i ii A mis padres A Xavi iii iv Acknowledgements This thesis has been made possible by the support of many people who in one way or another, many times unknowingly, gave me the strength to overcome this "long and winding road". First of all, I would like to thank my supervisor, Dr. Francesc Piferrer, for his patience, guidance and wise advice throughout all this Ph.D. experience. But above all, for the trust he placed on me almost seven years ago when he offered me the opportunity to be part of his team. Thanks also for teaching me how to question always everything, for sharing with me your enthusiasm for science and for giving me the opportunity of learning from you by participating in many projects, collaborations and scientific meetings. I am also thankful to my colleagues (former and present Group of Biology of Reproduction members) for your support and encouragement throughout this journey. To the “exGBRs”, thanks for helping me with my first steps into this world. Working as an undergrad with you Dr.
    [Show full text]
  • Identification of QTL for Resistance Against Two Fungal Pathogens, Pyrenophora Teres F
    Fluturë Novakazi Institut für Resistenzforschung und Stresstoleranz Identification of QTL for resistance against two fungal pathogens, Pyrenophora teres f. teres and Bipolaris sorokiniana, in a barley (Hordeum vulgare L.) diversity set Dissertationen aus dem Julius Kühn-Institut Julius Kühn-Institut Bundesforschungsinstitut für Kulturpflanzen Kontakt | Contact: Fluturë Novakazi Beethovenstraße 24 18069 Rostock Die Schriftenreihe ,,Dissertationen aus dem Julius Kühn-lnstitut“ veröffentlicht Doktorarbeiten, die in enger Zusammenarbeit mit Universitäten an lnstituten des Julius Kühn-lnstituts entstan- den sind. The publication series „Dissertationen aus dem Julius Kühn-lnstitut“ publishes doctoral disser- tations originating from research doctorates and completed at the Julius Kühn-Institut (JKI) either in close collaboration with universities or as an outstanding independent work in the JKI research fields. Der Vertrieb dieser Monographien erfolgt über den Buchhandel (Nachweis im Verzeichnis liefer- barer Bücher - VLB) und OPEN ACCESS unter: https://www.openagrar.de/receive/openagrar_mods_00005667 The monographs are distributed through the book trade (listed in German Books in Print - VLB) and OPEN ACCESS here: https://www.openagrar.de/receive/openagrar_mods_00005667 Wir unterstützen den offenen Zugang zu wissenschaftlichem Wissen. Die Dissertationen aus dem Julius Kühn-lnstitut erscheinen daher OPEN ACCESS. Alle Ausgaben stehen kostenfrei im lnternet zur Verfügung: http://www.julius-kuehn.de Bereich Veröffentlichungen. We advocate open access to scientific knowledge. Dissertations from the Julius Kühn-lnstitut are therefore published open access. All issues are available free of charge under http://www.julius-kuehn.de (see Publications). Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verzeichnet diese Publikation In der Deutschen Nationalbibliografie: detaillierte bibliografische Daten sind im lnternet über http://dnb.d-nb.de abrufbar.
    [Show full text]
  • Appendix 2. Significantly Differentially Regulated Genes in Term Compared with Second Trimester Amniotic Fluid Supernatant
    Appendix 2. Significantly Differentially Regulated Genes in Term Compared With Second Trimester Amniotic Fluid Supernatant Fold Change in term vs second trimester Amniotic Affymetrix Duplicate Fluid Probe ID probes Symbol Entrez Gene Name 1019.9 217059_at D MUC7 mucin 7, secreted 424.5 211735_x_at D SFTPC surfactant protein C 416.2 206835_at STATH statherin 363.4 214387_x_at D SFTPC surfactant protein C 295.5 205982_x_at D SFTPC surfactant protein C 288.7 1553454_at RPTN repetin solute carrier family 34 (sodium 251.3 204124_at SLC34A2 phosphate), member 2 238.9 206786_at HTN3 histatin 3 161.5 220191_at GKN1 gastrokine 1 152.7 223678_s_at D SFTPA2 surfactant protein A2 130.9 207430_s_at D MSMB microseminoprotein, beta- 99.0 214199_at SFTPD surfactant protein D major histocompatibility complex, class II, 96.5 210982_s_at D HLA-DRA DR alpha 96.5 221133_s_at D CLDN18 claudin 18 94.4 238222_at GKN2 gastrokine 2 93.7 1557961_s_at D LOC100127983 uncharacterized LOC100127983 93.1 229584_at LRRK2 leucine-rich repeat kinase 2 HOXD cluster antisense RNA 1 (non- 88.6 242042_s_at D HOXD-AS1 protein coding) 86.0 205569_at LAMP3 lysosomal-associated membrane protein 3 85.4 232698_at BPIFB2 BPI fold containing family B, member 2 84.4 205979_at SCGB2A1 secretoglobin, family 2A, member 1 84.3 230469_at RTKN2 rhotekin 2 82.2 204130_at HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2 81.9 222242_s_at KLK5 kallikrein-related peptidase 5 77.0 237281_at AKAP14 A kinase (PRKA) anchor protein 14 76.7 1553602_at MUCL1 mucin-like 1 76.3 216359_at D MUC7 mucin 7,
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2014/0304845 A1 Loboda Et Al
    US 201403.04845A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2014/0304845 A1 Loboda et al. (43) Pub. Date: Oct. 9, 2014 54) ALZHEMIERS DISEASE SIGNATURE Publicationublication ClassificatiClassification MARKERS AND METHODS OF USE (51) Int. Cl. (71) Applicant: MERCKSHARP & DOHME CORP, CI2O I/68 (2006.01) Rahway, NJ (US) AOIK 67/027 (2006.01) (52) U.S. Cl. (72) Inventors: SIES yet.sS); CPC .......... CI2O I/6883 (2013.01); A0IK 67/0275 Icnaei NepoZnyn, Yeadon, (2013.01) le.East,Italia; David J. Stone, Wyncote, USPC .............. 800/12:536/23.5:536/23.2:506/17 (US); Keith Tanis, Quakertown, PA (US); William J. Ray, Juniper, FL (US) (57) ABSTRACT (21) Appl. No.: 14/354,622 Methods, biomarkers, and expression signatures are dis 1-1. closed for assessing the disease progression of Alzheimer's (22) PCT Filed: Oct. 26, 2012 disease (AD). In one embodiment, BioAge (biological age), NdStress (neurodegenerative stress), Alz (Alzheimer), and (86). PCT No.: PCT/US12A62218 Inflame (inflammation) are used as biomarkers of AD pro S371 (c)(1), gression. In another aspect, the invention comprises a gene (2), (4) Date: Apr. 28, 2014 signature for evaluating disease progression. In still another Related U.S. Application Data abiliticises in (60) Provisional application No. 61/553,400, filed on Oct. used to identify animal models for use in the development and 31, 2011. evaluation of therapeutics for the treatment of AD. Patent Application Publication Oct. 9, 2014 Sheet 1 of 16 US 2014/0304845 A1 Y : : O O O O O O O O D O v v CN On cy Patent Application Publication Oct.
    [Show full text]
  • Supp Table 6.Pdf
    Supplementary Table 6. Processes associated to the 2037 SCL candidate target genes ID Symbol Entrez Gene Name Process NM_178114 AMIGO2 adhesion molecule with Ig-like domain 2 adhesion NM_033474 ARVCF armadillo repeat gene deletes in velocardiofacial syndrome adhesion NM_027060 BTBD9 BTB (POZ) domain containing 9 adhesion NM_001039149 CD226 CD226 molecule adhesion NM_010581 CD47 CD47 molecule adhesion NM_023370 CDH23 cadherin-like 23 adhesion NM_207298 CERCAM cerebral endothelial cell adhesion molecule adhesion NM_021719 CLDN15 claudin 15 adhesion NM_009902 CLDN3 claudin 3 adhesion NM_008779 CNTN3 contactin 3 (plasmacytoma associated) adhesion NM_015734 COL5A1 collagen, type V, alpha 1 adhesion NM_007803 CTTN cortactin adhesion NM_009142 CX3CL1 chemokine (C-X3-C motif) ligand 1 adhesion NM_031174 DSCAM Down syndrome cell adhesion molecule adhesion NM_145158 EMILIN2 elastin microfibril interfacer 2 adhesion NM_001081286 FAT1 FAT tumor suppressor homolog 1 (Drosophila) adhesion NM_001080814 FAT3 FAT tumor suppressor homolog 3 (Drosophila) adhesion NM_153795 FERMT3 fermitin family homolog 3 (Drosophila) adhesion NM_010494 ICAM2 intercellular adhesion molecule 2 adhesion NM_023892 ICAM4 (includes EG:3386) intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)adhesion NM_001001979 MEGF10 multiple EGF-like-domains 10 adhesion NM_172522 MEGF11 multiple EGF-like-domains 11 adhesion NM_010739 MUC13 mucin 13, cell surface associated adhesion NM_013610 NINJ1 ninjurin 1 adhesion NM_016718 NINJ2 ninjurin 2 adhesion NM_172932 NLGN3 neuroligin
    [Show full text]
  • Human Induced Pluripotent Stem Cell–Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation
    BASIC RESEARCH www.jasn.org Human Induced Pluripotent Stem Cell–Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation † Sazia Sharmin,* Atsuhiro Taguchi,* Yusuke Kaku,* Yasuhiro Yoshimura,* Tomoko Ohmori,* ‡ † ‡ Tetsushi Sakuma, Masashi Mukoyama, Takashi Yamamoto, Hidetake Kurihara,§ and | Ryuichi Nishinakamura* *Department of Kidney Development, Institute of Molecular Embryology and Genetics, and †Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan; ‡Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan; §Division of Anatomy, Juntendo University School of Medicine, Tokyo, Japan; and |Japan Science and Technology Agency, CREST, Kumamoto, Japan ABSTRACT Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator–like effector nuclease-mediated homologous recombination, we generated human iPS cell lines that express green fluorescent protein (GFP) in the NPHS1 locus, which encodes nephrin, and we show that GFP expression facilitated accurate visualization of nephrin-positive podocyte formation in
    [Show full text]
  • In: O Guru, O Iniciador E Outras Variações
    \ FREDRIK BARTH [organização: Tomke Lask 1 ColeçãokjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBAOypog1'apbos Direção:Antonio Carlos deSouza Lima I. Processos e escolhas: estudos de sociologia política Elisa Pereira Reis 2. O guru, o iniciador e outras variações antropológicas o guru) O iniciador Frtdrik Barth [organizarão: Tomk, LaskPONMLKJIHGFEDCBA1 e outras variações antropológicas 3. Criatividade social, subjetividade coletiva ;]A e a modernidade brasileira contemporânea !~f.,<if+J l/;.-, v [os! Maurício Domingues UNIDADE ..L J... t; I /.:z..r:tJ~J. NA CHAMADA 501,''-=+' "( t-I"f G? <.:\,;.:.\ " ..•". '.'''l..,. "r,y T V __ .__ ~EX ;~~~1K'8~ C~ DO PRECO ~ ~2. DATA 170 - O l: Tradução l-l'-I'I 6 q ~ t John Cunha Comeiford :11"10107l~403 1"" "1 «., r.IE 301.28282g /,,- c· ~~fp ( c. lJi\liCAlP ~~~SiJ.;!-!rlç.~Cl! .•. Copyrighc Cl 2000. Fredrik Barrh SumáriokjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA TrtHiUfio Jobn Cunba Comerjord IMutrc t Oouloundo pelo PPGAS/Muscu N~.:ionJIJ R,yistio ütnic« Apresentação - Tomke Lask 7 Antonio Csrlo: deSouza Lima Projeto Cr4fieo l prtp.,llfio . Os grupos étnicos e suas fronteiras 25 Contra Capa Aidentidade pathan esuamanutenção 69 Processos étnicos na fronteira entre os Pathan e os Baluchi 95PONMLKJIHGFEDCBA BARTH. FredrikA J O guru. o iniciador e outras variações antropolôgicas. - Fredrik Buth. A análise da cultura nas sociedades complexas 107 Tradução de John Cunha Comerford. Rio de [aneiro: Contra C'P' Livrari•. 2000. O guru e o iniciador: transações de conhecimento e [lj'pographos n" 2] - 2H p.: 1+ • 21 cm. moldagem da cultura no sudeste da Ásia e na Melanésia 141 Por um maior naturalismo na conceptualização das sociedades 167 Inclui Bibliografi a.
    [Show full text]
  • On SARS-Cov-2, Tropical Medicine and Bioinformatics: Analysis of the SARS-Cov-2 Molecular Features and Epitope Prediction for Antibody Or Vaccine Development
    ACTA SCIENTIFIC MICROBIOLOGY (ISSN: 2581-3226) Volume 4 Issue 1 January 2021 Research Article On SARS-CoV-2, Tropical Medicine and Bioinformatics: Analysis of the SARS-Cov-2 Molecular Features and Epitope Prediction for Antibody or Vaccine Development Joshua Angelo Hermida Mandanas* Received: November 24, 2020 Tropical Medicine Scientist and Immunobiologist, Philippine Medical Technology Published: December 08, 2020 Professional/National Lecturer, University of the Philippines Manila, Philippines © All rights are reserved by Joshua Angelo *Corresponding Author: Joshua Angelo Hermida Mandanas, Tropical Medicine Hermida Mandanas. Scientist and Immunobiologist, Philippine Medical Technology Professional/National Lecturer, University of the Philippines Manila, Philippines. Abstract Introduction: SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the cause of COVID-19 which is the pandemic as of the current time. It is a global public health emergency and is still on the loose of spreading more infections and deaths. Bioinformat- ics offer extensive visualization and analysis of combined molecular, cellular, biochemical and immunobiologic aspects of SARS-CoV-2 which is indeed vital for antibody and vaccine development. Objectives: This paper presents the SARS-CoV-2 molecular virologic, biochemical, cellular and immunobiologic features and gives a basic B cell linear epitope prediction of the SARS-CoV-2 spike (S) glycoprotein using bioinformatics which both can serve as a guide for development of vaccines or antibody-based treatments. Methods: Several bioinformatic methods were used, from sequence analysis (Uniprot), structural correlation (PDB), molecular mod- elling (UCSF Chimera) and illustration (Biorender), B cell linear epitope prediction tools, conservancy analysis and search of related epitopes (IEDB) together with determination of disordered regions (GlobPlot and DisEMBL).
    [Show full text]
  • Technical Issues in Measuring Dropout in Primary Schools in Quetta Aamara Bibi Mrs Amir Bano Mr. Abdul Wahab Abstract
    1 Bi-Annual Research Journal “Journal of Education & Humanities Research” ISSN: 2415-2366 Institute of Education and Research (IER), UOB, Quetta Pakistan Vol.6.NO 2, 2018 Technical Issues in Measuring Dropout In Primary Schools In Quetta Aamara Bibi M.Phil. Scholar. Balochistan Study Center, University of Balochistan, Quetta. Mrs Amir Bano Assistant Professor. IER, University of Balochistan, Quetta. Mr. Abdul Wahab Lecturer, IER, University of Balochistan, Quetta. Abstract: The issue of Primary school dropouts has produced expanded interest among Teachers, policymakers and Researchers especially in last few years. many technical issued are being faced in measuring this social phenomenon This Quantitative study on technical issues in measuring dropout looks at the technical issues faced into comprehend and understand this social and instructive issue which has direct impacts on society. To figure out the issue two areas have been focused for covering the data through self-constructed questionnaire , first random sampling with (50) fifty primary school Head teachers and second with stratified random sampling by educational administrators in Quetta. The data analysis tool based on Statistical Package for the Social Sciences (SPSS) which is assisted in measuring the data in descriptive and cluster form. Results of the study with discussion identified crucial finding especially for the future research and helpful paper for educational administration of Balochistan to revisit the policies regarding measuring method of dropout. The recommendations and implications are also discussed at the end of this study. Key Words. Technical issues. Dropout. Primary schools. Quetta. 2 Introduction Education with no doubt universally is considered as key for development in socio-economic conscious countries.
    [Show full text]